{"action":"created","issue":{"url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561","repository_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List","labels_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561/labels{/name}","comments_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561/comments","events_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561/events","html_url":"https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/561","id":301759950,"node_id":"MDU6SXNzdWUzMDE3NTk5NTA=","number":561,"title":"Experimental identification of target for Series 3","user":{"login":"holeung","id":10951570,"node_id":"MDQ6VXNlcjEwOTUxNTcw","avatar_url":"https://avatars3.githubusercontent.com/u/10951570?v=4","gravatar_id":"","url":"https://api.github.com/users/holeung","html_url":"https://github.com/holeung","followers_url":"https://api.github.com/users/holeung/followers","following_url":"https://api.github.com/users/holeung/following{/other_user}","gists_url":"https://api.github.com/users/holeung/gists{/gist_id}","starred_url":"https://api.github.com/users/holeung/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/holeung/subscriptions","organizations_url":"https://api.github.com/users/holeung/orgs","repos_url":"https://api.github.com/users/holeung/repos","events_url":"https://api.github.com/users/holeung/events{/privacy}","received_events_url":"https://api.github.com/users/holeung/received_events","type":"User","site_admin":false},"labels":[{"id":39186424,"node_id":"MDU6TGFiZWwzOTE4NjQyNA==","url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/labels/Collaboration%20Request","name":"Collaboration Request","color":"fbca04","default":false},{"id":78262189,"node_id":"MDU6TGFiZWw3ODI2MjE4OQ==","url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/labels/Series%203","name":"Series 3","color":"2EFE64","default":false}],"state":"open","locked":false,"assignee":{"login":"holeung","id":10951570,"node_id":"MDQ6VXNlcjEwOTUxNTcw","avatar_url":"https://avatars3.githubusercontent.com/u/10951570?v=4","gravatar_id":"","url":"https://api.github.com/users/holeung","html_url":"https://github.com/holeung","followers_url":"https://api.github.com/users/holeung/followers","following_url":"https://api.github.com/users/holeung/following{/other_user}","gists_url":"https://api.github.com/users/holeung/gists{/gist_id}","starred_url":"https://api.github.com/users/holeung/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/holeung/subscriptions","organizations_url":"https://api.github.com/users/holeung/orgs","repos_url":"https://api.github.com/users/holeung/repos","events_url":"https://api.github.com/users/holeung/events{/privacy}","received_events_url":"https://api.github.com/users/holeung/received_events","type":"User","site_admin":false},"assignees":[{"login":"holeung","id":10951570,"node_id":"MDQ6VXNlcjEwOTUxNTcw","avatar_url":"https://avatars3.githubusercontent.com/u/10951570?v=4","gravatar_id":"","url":"https://api.github.com/users/holeung","html_url":"https://github.com/holeung","followers_url":"https://api.github.com/users/holeung/followers","following_url":"https://api.github.com/users/holeung/following{/other_user}","gists_url":"https://api.github.com/users/holeung/gists{/gist_id}","starred_url":"https://api.github.com/users/holeung/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/holeung/subscriptions","organizations_url":"https://api.github.com/users/holeung/orgs","repos_url":"https://api.github.com/users/holeung/repos","events_url":"https://api.github.com/users/holeung/events{/privacy}","received_events_url":"https://api.github.com/users/holeung/received_events","type":"User","site_admin":false}],"milestone":null,"comments":49,"created_at":"2018-03-02T13:04:58Z","updated_at":"2018-06-26T20:52:33Z","closed_at":null,"author_association":"MEMBER","body":"I received a small grant to support experimental, biochemical testing of potential targets for Series 3 (#549) through partnerships with the wonderful core labs (NMR, mass spectrometry, X-ray crystallography, protein expression) of the University of Kansas.\r\n\r\nCurrent approaches that we discussed include:\r\n1. Recombinant expression of candidate kinase/kinase-like targets as discussed in #549. We would prefer to start with those with published expression protocols and that can be expressed in E. coli. We would test for inhibitor binding by saturation transfer difference NMR. We would start by testing our top 5 candidates.\r\n\r\n2. Screening of inhibitors against fractions of Plasmodium lysate or enriched lysate, preferably from a non-infectious Plasmodium species or sterilized P. falciparum. We would screen by STD NMR, affinity mass spectrometry, or by something like DARTS ([https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442491/](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442491)). Anyone know of a source of Plasmodium lysate?\r\n\r\n3. Your idea here! Note that target identification is considered difficult and non-routine.\r\n\r\nI will keep this sub-project open and under the umbrella and spirit of OSM."},"comment":{"url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/comments/400458285","html_url":"https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/561#issuecomment-400458285","issue_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561","id":400458285,"node_id":"MDEyOklzc3VlQ29tbWVudDQwMDQ1ODI4NQ==","user":{"login":"mpollastri","id":38247259,"node_id":"MDQ6VXNlcjM4MjQ3MjU5","avatar_url":"https://avatars2.githubusercontent.com/u/38247259?v=4","gravatar_id":"","url":"https://api.github.com/users/mpollastri","html_url":"https://github.com/mpollastri","followers_url":"https://api.github.com/users/mpollastri/followers","following_url":"https://api.github.com/users/mpollastri/following{/other_user}","gists_url":"https://api.github.com/users/mpollastri/gists{/gist_id}","starred_url":"https://api.github.com/users/mpollastri/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mpollastri/subscriptions","organizations_url":"https://api.github.com/users/mpollastri/orgs","repos_url":"https://api.github.com/users/mpollastri/repos","events_url":"https://api.github.com/users/mpollastri/events{/privacy}","received_events_url":"https://api.github.com/users/mpollastri/received_events","type":"User","site_admin":false},"created_at":"2018-06-26T20:52:32Z","updated_at":"2018-06-26T20:52:32Z","author_association":"NONE","body":"The primary issue with CA is one of selectivity versus host. It’s tricky sometimes. Not that this compound should be avoided or dropped, but go into it with eyes open!\r\n\r\nFrom: Chris Swain [mailto:notifications@github.com]\r\nSent: Tuesday, June 26, 2018 4:11 PM\r\nTo: OpenSourceMalaria/OSM_To_Do_List <OSM_To_Do_List@noreply.github.com>\r\nCc: Pollastri, Michael <M.Pollastri@northeastern.edu>; Mention <mention@noreply.github.com>\r\nSubject: Re: [OpenSourceMalaria/OSM_To_Do_List] Experimental identification of target for Series 3 (#561)\r\n\r\n\r\n@MFernflower<https://github.com/MFernflower> Indeed, and there are a number of more recent publications suggesting CA as a good target. This together with the extensive availability of known CA inhibitors raises the question what is the stumbling block. @PaulWillisMMV<https://github.com/PaulWillisMMV> @mattodd<https://github.com/mattodd> is there a particular problem with this approach?\r\n\r\n—\r\nYou are receiving this because you were mentioned.\r\nReply to this email directly, view it on GitHub<https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/561#issuecomment-400445600>, or mute the thread<https://github.com/notifications/unsubscribe-auth/AkebW7FpIRTz6GvaVi9MnJZ_qqWVsHcHks5uApVNgaJpZM4SZ3Um>.\r\n"}}
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