{"action":"created","issue":{"url":"https://api.github.com/repos/ndierckx/NOVOPlasty/issues/65","repository_url":"https://api.github.com/repos/ndierckx/NOVOPlasty","labels_url":"https://api.github.com/repos/ndierckx/NOVOPlasty/issues/65/labels{/name}","comments_url":"https://api.github.com/repos/ndierckx/NOVOPlasty/issues/65/comments","events_url":"https://api.github.com/repos/ndierckx/NOVOPlasty/issues/65/events","html_url":"https://github.com/ndierckx/NOVOPlasty/issues/65","id":369751586,"node_id":"MDU6SXNzdWUzNjk3NTE1ODY=","number":65,"title":"very few reads assembling","user":{"login":"jullee","id":11912259,"node_id":"MDQ6VXNlcjExOTEyMjU5","avatar_url":"https://avatars0.githubusercontent.com/u/11912259?v=4","gravatar_id":"","url":"https://api.github.com/users/jullee","html_url":"https://github.com/jullee","followers_url":"https://api.github.com/users/jullee/followers","following_url":"https://api.github.com/users/jullee/following{/other_user}","gists_url":"https://api.github.com/users/jullee/gists{/gist_id}","starred_url":"https://api.github.com/users/jullee/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/jullee/subscriptions","organizations_url":"https://api.github.com/users/jullee/orgs","repos_url":"https://api.github.com/users/jullee/repos","events_url":"https://api.github.com/users/jullee/events{/privacy}","received_events_url":"https://api.github.com/users/jullee/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2018-10-13T00:58:07Z","updated_at":"2018-10-13T01:17:28Z","closed_at":null,"author_association":"NONE","body":"I'm stumped over the results I'm getting from the program (vs. 2.7.2) when trying to assemble the chloroplast. \r\n\r\nI provide a reference chloroplast from the same species (151 000 bp) and use the rbcL gene as the seed. The program runs but produces only one very small contig and the output says only 22 reads have been assembled. The reads are untrimmed (only adaptor removed with Trimmomatic) fastq.gz files, and only reads with a pair have been provided (unpaired reads output from Trimmomatic were not included). Curious why this might be happening? We have decent coverage for the nuclear genome so I doubt its a coverage issue...\r\n\r\nMy log file is \r\n[log_Test_cp_ANN01.txt](https://github.com/ndierckx/NOVOPlasty/files/2474938/log_Test_cp_ANN01.txt)\r\nincluded. Any help would be greatly appreciated."},"comment":{"url":"https://api.github.com/repos/ndierckx/NOVOPlasty/issues/comments/429499623","html_url":"https://github.com/ndierckx/NOVOPlasty/issues/65#issuecomment-429499623","issue_url":"https://api.github.com/repos/ndierckx/NOVOPlasty/issues/65","id":429499623,"node_id":"MDEyOklzc3VlQ29tbWVudDQyOTQ5OTYyMw==","user":{"login":"ndierckx","id":15016981,"node_id":"MDQ6VXNlcjE1MDE2OTgx","avatar_url":"https://avatars3.githubusercontent.com/u/15016981?v=4","gravatar_id":"","url":"https://api.github.com/users/ndierckx","html_url":"https://github.com/ndierckx","followers_url":"https://api.github.com/users/ndierckx/followers","following_url":"https://api.github.com/users/ndierckx/following{/other_user}","gists_url":"https://api.github.com/users/ndierckx/gists{/gist_id}","starred_url":"https://api.github.com/users/ndierckx/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ndierckx/subscriptions","organizations_url":"https://api.github.com/users/ndierckx/orgs","repos_url":"https://api.github.com/users/ndierckx/repos","events_url":"https://api.github.com/users/ndierckx/events{/privacy}","received_events_url":"https://api.github.com/users/ndierckx/received_events","type":"User","site_admin":false},"created_at":"2018-10-13T01:17:28Z","updated_at":"2018-10-13T01:17:28Z","author_association":"OWNER","body":"You have put max memory to 1 so subsampled only 5 % of your data. You must have more than 1 GB of RAM available?"}}
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