{"action":"created","issue":{"url":"https://api.github.com/repos/benjjneb/dada2/issues/576","repository_url":"https://api.github.com/repos/benjjneb/dada2","labels_url":"https://api.github.com/repos/benjjneb/dada2/issues/576/labels{/name}","comments_url":"https://api.github.com/repos/benjjneb/dada2/issues/576/comments","events_url":"https://api.github.com/repos/benjjneb/dada2/issues/576/events","html_url":"https://github.com/benjjneb/dada2/issues/576","id":366024827,"node_id":"MDU6SXNzdWUzNjYwMjQ4Mjc=","number":576,"title":"Using DADA2 with short barcode sequences","user":{"login":"apcamargo","id":22940964,"node_id":"MDQ6VXNlcjIyOTQwOTY0","avatar_url":"https://avatars2.githubusercontent.com/u/22940964?v=4","gravatar_id":"","url":"https://api.github.com/users/apcamargo","html_url":"https://github.com/apcamargo","followers_url":"https://api.github.com/users/apcamargo/followers","following_url":"https://api.github.com/users/apcamargo/following{/other_user}","gists_url":"https://api.github.com/users/apcamargo/gists{/gist_id}","starred_url":"https://api.github.com/users/apcamargo/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/apcamargo/subscriptions","organizations_url":"https://api.github.com/users/apcamargo/orgs","repos_url":"https://api.github.com/users/apcamargo/repos","events_url":"https://api.github.com/users/apcamargo/events{/privacy}","received_events_url":"https://api.github.com/users/apcamargo/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":8,"created_at":"2018-10-02T18:31:33Z","updated_at":"2018-10-08T21:51:28Z","closed_at":null,"author_association":"NONE","body":"I'm planning to use DADA2 to denoise sequencing data of a very short barcode sequence (20bp). Aside from changing the minLen parameter is there anything else I should do?\r\n\r\nThank you for your attention."},"comment":{"url":"https://api.github.com/repos/benjjneb/dada2/issues/comments/427990898","html_url":"https://github.com/benjjneb/dada2/issues/576#issuecomment-427990898","issue_url":"https://api.github.com/repos/benjjneb/dada2/issues/576","id":427990898,"node_id":"MDEyOklzc3VlQ29tbWVudDQyNzk5MDg5OA==","user":{"login":"apcamargo","id":22940964,"node_id":"MDQ6VXNlcjIyOTQwOTY0","avatar_url":"https://avatars2.githubusercontent.com/u/22940964?v=4","gravatar_id":"","url":"https://api.github.com/users/apcamargo","html_url":"https://github.com/apcamargo","followers_url":"https://api.github.com/users/apcamargo/followers","following_url":"https://api.github.com/users/apcamargo/following{/other_user}","gists_url":"https://api.github.com/users/apcamargo/gists{/gist_id}","starred_url":"https://api.github.com/users/apcamargo/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/apcamargo/subscriptions","organizations_url":"https://api.github.com/users/apcamargo/orgs","repos_url":"https://api.github.com/users/apcamargo/repos","events_url":"https://api.github.com/users/apcamargo/events{/privacy}","received_events_url":"https://api.github.com/users/apcamargo/received_events","type":"User","site_admin":false},"created_at":"2018-10-08T21:51:28Z","updated_at":"2018-10-08T21:51:28Z","author_association":"NONE","body":"Is there any possibility that DADA2 erroneously detected that my sequences are ASCII base 64 instead of base 33? Most of the sequences have Q=41 in all their bases (so, the FASTQ is full of \"J\"s). Maybe the algorithm is processing my sequences as if they are PHRED64, in which \"J\" would be Q=10.\r\n\r\nI've had this problem before with DADA2. It removed all my reads during filtering because they were precessed as bad quality reads."}}
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