{"action":"opened","issue":{"url":"https://api.github.com/repos/ParBLiSS/FastANI/issues/33","repository_url":"https://api.github.com/repos/ParBLiSS/FastANI","labels_url":"https://api.github.com/repos/ParBLiSS/FastANI/issues/33/labels{/name}","comments_url":"https://api.github.com/repos/ParBLiSS/FastANI/issues/33/comments","events_url":"https://api.github.com/repos/ParBLiSS/FastANI/issues/33/events","html_url":"https://github.com/ParBLiSS/FastANI/issues/33","id":391518592,"node_id":"MDU6SXNzdWUzOTE1MTg1OTI=","number":33,"title":"ERROR, skch::validateInputFiles","user":{"login":"vinisalazar","id":17276653,"node_id":"MDQ6VXNlcjE3Mjc2NjUz","avatar_url":"https://avatars2.githubusercontent.com/u/17276653?v=4","gravatar_id":"","url":"https://api.github.com/users/vinisalazar","html_url":"https://github.com/vinisalazar","followers_url":"https://api.github.com/users/vinisalazar/followers","following_url":"https://api.github.com/users/vinisalazar/following{/other_user}","gists_url":"https://api.github.com/users/vinisalazar/gists{/gist_id}","starred_url":"https://api.github.com/users/vinisalazar/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/vinisalazar/subscriptions","organizations_url":"https://api.github.com/users/vinisalazar/orgs","repos_url":"https://api.github.com/users/vinisalazar/repos","events_url":"https://api.github.com/users/vinisalazar/events{/privacy}","received_events_url":"https://api.github.com/users/vinisalazar/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2018-12-16T23:51:17Z","updated_at":"2018-12-16T23:51:17Z","closed_at":null,"author_association":"NONE","body":"I'm getting the following error when trying to run FastANI:\r\n```\r\n>>>>>>>>>>>>>>>>>>\r\nReference = [/Users/viniWS/Bio/gtdb/fasta/NC_006576.1.fasta, /Users/viniWS/Bio/gtdb/fasta/NC_007335.2.fasta, /Users/viniWS/Bio/gtdb/fasta/NC_007513.fasta]\r\nQuery = [Bio/gtdb/fasta/NC_005042.fna]\r\nKmer size = 16\r\nFragment length = 3000\r\nThreads = 1\r\nANI output file = Bio/gtdb/data/ani_test_out.txt\r\n>>>>>>>>>>>>>>>>>>\r\nERROR, skch::validateInputFiles, Could not open /Users/viniWS/Bio/gtdb/fasta/NC_007513.fasta\r\n```\r\n\r\nThis is the content for `head /Users/viniWS/Bio/gtdb/fasta/NC_007513.fasta`:\r\n```\r\n>NC_005042.1 Prochlorococcus marinus subsp. marinus str. CCMP1375 complete genome\r\nAAAGCTAGATGGCAGAAAGGTTTTTGAATAATTTCCACAGATTCCACAAGACCTACTACT\r\nACTGTATTAATTTCATATAATTAAATTAGAATTACTAGAAGAAGAGAAAACTTTTATTAA\r\nAGCTATGAAAACTTTTTGTTCCTTTTTTGGTATTTCGTAGTTATGTTGAACCGATGAAAC\r\nTTGTTTGTTCTCAAATTGAGCTCAATACAGCTCTTCAACTAGTTAGTAGAGCTGTAGCCA\r\nCTAGGCCTTCGCATCCAGTATTGGCAAATGTATTGCTTACTGCTGATGCGGGAACTGGAA\r\nAACTAAGCTTAACTGGATTTGATCTTAATTTAGGAATTCAAACATCGCTTAGTGCTTCTA\r\nTCGAAAGTAGTGGAGCAATTACAGTTCCCTCAAAACTTTTCGGAGAAATAATATCAAAAT\r\nTATCTAGTGAATCTTCTATAACTTTATCAACAGATGATTCTAGTGAACAAGTTAATTTAA\r\nAGAGTAAAAGTGGAAATTATCAGGTAAGAGCTATGAGTGCTGATGATTTTCCTGATTTGC\r\n```\r\n\r\nThis is the content of `Bio/gtdb/data/ani_test.txt`:\r\n```\r\n/Users/viniWS/Bio/gtdb/fasta/NC_006576.1.fasta\r\n/Users/viniWS/Bio/gtdb/fasta/NC_007335.2.fasta\r\n/Users/viniWS/Bio/gtdb/fasta/NC_007513.fasta\r\n```\r\n\r\nAny ideas why this might be happening? I did some test runs on small-ish fragments (1500 bp) and it worked fine, but when using 'real world' genomes, I always get this error.\r\n\r\n"}}
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