{"action":"created","issue":{"url":"https://api.github.com/repos/sr320/course-fish546-2018/issues/7","repository_url":"https://api.github.com/repos/sr320/course-fish546-2018","labels_url":"https://api.github.com/repos/sr320/course-fish546-2018/issues/7/labels{/name}","comments_url":"https://api.github.com/repos/sr320/course-fish546-2018/issues/7/comments","events_url":"https://api.github.com/repos/sr320/course-fish546-2018/issues/7/events","html_url":"https://github.com/sr320/course-fish546-2018/issues/7","id":364585190,"node_id":"MDU6SXNzdWUzNjQ1ODUxOTA=","number":7,"title":"Project Ideas","user":{"login":"sr320","id":4442039,"node_id":"MDQ6VXNlcjQ0NDIwMzk=","avatar_url":"https://avatars1.githubusercontent.com/u/4442039?v=4","gravatar_id":"","url":"https://api.github.com/users/sr320","html_url":"https://github.com/sr320","followers_url":"https://api.github.com/users/sr320/followers","following_url":"https://api.github.com/users/sr320/following{/other_user}","gists_url":"https://api.github.com/users/sr320/gists{/gist_id}","starred_url":"https://api.github.com/users/sr320/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/sr320/subscriptions","organizations_url":"https://api.github.com/users/sr320/orgs","repos_url":"https://api.github.com/users/sr320/repos","events_url":"https://api.github.com/users/sr320/events{/privacy}","received_events_url":"https://api.github.com/users/sr320/received_events","type":"User","site_admin":false},"labels":[{"id":1061153455,"node_id":"MDU6TGFiZWwxMDYxMTUzNDU1","url":"https://api.github.com/repos/sr320/course-fish546-2018/labels/quiz","name":"quiz","color":"7ffc0a","default":false}],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":{"url":"https://api.github.com/repos/sr320/course-fish546-2018/milestones/2","html_url":"https://github.com/sr320/course-fish546-2018/milestone/2","labels_url":"https://api.github.com/repos/sr320/course-fish546-2018/milestones/2/labels","id":3694466,"node_id":"MDk6TWlsZXN0b25lMzY5NDQ2Ng==","number":2,"title":"Week 1","description":"","creator":{"login":"sr320","id":4442039,"node_id":"MDQ6VXNlcjQ0NDIwMzk=","avatar_url":"https://avatars1.githubusercontent.com/u/4442039?v=4","gravatar_id":"","url":"https://api.github.com/users/sr320","html_url":"https://github.com/sr320","followers_url":"https://api.github.com/users/sr320/followers","following_url":"https://api.github.com/users/sr320/following{/other_user}","gists_url":"https://api.github.com/users/sr320/gists{/gist_id}","starred_url":"https://api.github.com/users/sr320/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/sr320/subscriptions","organizations_url":"https://api.github.com/users/sr320/orgs","repos_url":"https://api.github.com/users/sr320/repos","events_url":"https://api.github.com/users/sr320/events{/privacy}","received_events_url":"https://api.github.com/users/sr320/received_events","type":"User","site_admin":false},"open_issues":4,"closed_issues":0,"state":"open","created_at":"2018-09-27T17:39:27Z","updated_at":"2018-09-28T16:02:30Z","due_on":"2018-10-05T07:00:00Z","closed_at":null},"comments":9,"created_at":"2018-09-27T17:47:03Z","updated_at":"2018-10-05T21:18:09Z","closed_at":null,"author_association":"OWNER","body":"As I mentioned, each student will have a \"project\" that results in a \"product\". Do you have any preference to what such project is involved with? What is one idea that comes to mind based on the reading and your interests. Please include any questions or concerns."},"comment":{"url":"https://api.github.com/repos/sr320/course-fish546-2018/issues/comments/427501481","html_url":"https://github.com/sr320/course-fish546-2018/issues/7#issuecomment-427501481","issue_url":"https://api.github.com/repos/sr320/course-fish546-2018/issues/7","id":427501481,"node_id":"MDEyOklzc3VlQ29tbWVudDQyNzUwMTQ4MQ==","user":{"login":"laurahspencer","id":17264765,"node_id":"MDQ6VXNlcjE3MjY0NzY1","avatar_url":"https://avatars2.githubusercontent.com/u/17264765?v=4","gravatar_id":"","url":"https://api.github.com/users/laurahspencer","html_url":"https://github.com/laurahspencer","followers_url":"https://api.github.com/users/laurahspencer/followers","following_url":"https://api.github.com/users/laurahspencer/following{/other_user}","gists_url":"https://api.github.com/users/laurahspencer/gists{/gist_id}","starred_url":"https://api.github.com/users/laurahspencer/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/laurahspencer/subscriptions","organizations_url":"https://api.github.com/users/laurahspencer/orgs","repos_url":"https://api.github.com/users/laurahspencer/repos","events_url":"https://api.github.com/users/laurahspencer/events{/privacy}","received_events_url":"https://api.github.com/users/laurahspencer/received_events","type":"User","site_admin":false},"created_at":"2018-10-05T21:18:09Z","updated_at":"2018-10-05T21:18:09Z","author_association":"NONE","body":"I want to learn how to process RNA sequence data with the end goal of differential expression analysis between my oyster groups.  The samples I sent Katherine are listed on the bottom of this [GitHub issue](https://github.com/RobertsLab/project-olympia.oyster-genomic/issues/34#event-1497598684):  \r\n\r\nMy samples' QuantSeq data is delayed a few weeks, so Katherine is sending me a couple things I can play with to learn and develop pipelines:  \r\n  - Raw RNA full transcriptomic data that was sequenced with the first few gonad samples I sent her. I can use this data to learn how to assemble a transcriptome, which I can use in my mapping step. She is also assembling using the same data, so I could just use hers once it's ready.  \r\n  - Raw RNA QuantSeq data from her projects to learn how to demultiplex, map, QA/QC, quantify, differential expression .  \r\n\r\nKatherine also offered to send me her assembled transcriptome.  I have no idea how much time it will take for me to do the above, so interested in your assessment of what's feasible and makes most sense.   "}}
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