{"action":"created","issue":{"url":"https://api.github.com/repos/hammerlab/immune-infiltrate-explorations/issues/14","repository_url":"https://api.github.com/repos/hammerlab/immune-infiltrate-explorations","labels_url":"https://api.github.com/repos/hammerlab/immune-infiltrate-explorations/issues/14/labels{/name}","comments_url":"https://api.github.com/repos/hammerlab/immune-infiltrate-explorations/issues/14/comments","events_url":"https://api.github.com/repos/hammerlab/immune-infiltrate-explorations/issues/14/events","html_url":"https://github.com/hammerlab/immune-infiltrate-explorations/issues/14","id":192640011,"number":14,"title":"Generate data at MUSC","user":{"login":"hammer","id":15233,"avatar_url":"https://avatars.githubusercontent.com/u/15233?v=3","gravatar_id":"","url":"https://api.github.com/users/hammer","html_url":"https://github.com/hammer","followers_url":"https://api.github.com/users/hammer/followers","following_url":"https://api.github.com/users/hammer/following{/other_user}","gists_url":"https://api.github.com/users/hammer/gists{/gist_id}","starred_url":"https://api.github.com/users/hammer/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/hammer/subscriptions","organizations_url":"https://api.github.com/users/hammer/orgs","repos_url":"https://api.github.com/users/hammer/repos","events_url":"https://api.github.com/users/hammer/events{/privacy}","received_events_url":"https://api.github.com/users/hammer/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":{"login":"hammer","id":15233,"avatar_url":"https://avatars.githubusercontent.com/u/15233?v=3","gravatar_id":"","url":"https://api.github.com/users/hammer","html_url":"https://github.com/hammer","followers_url":"https://api.github.com/users/hammer/followers","following_url":"https://api.github.com/users/hammer/following{/other_user}","gists_url":"https://api.github.com/users/hammer/gists{/gist_id}","starred_url":"https://api.github.com/users/hammer/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/hammer/subscriptions","organizations_url":"https://api.github.com/users/hammer/orgs","repos_url":"https://api.github.com/users/hammer/repos","events_url":"https://api.github.com/users/hammer/events{/privacy}","received_events_url":"https://api.github.com/users/hammer/received_events","type":"User","site_admin":false},"assignees":[{"login":"hammer","id":15233,"avatar_url":"https://avatars.githubusercontent.com/u/15233?v=3","gravatar_id":"","url":"https://api.github.com/users/hammer","html_url":"https://github.com/hammer","followers_url":"https://api.github.com/users/hammer/followers","following_url":"https://api.github.com/users/hammer/following{/other_user}","gists_url":"https://api.github.com/users/hammer/gists{/gist_id}","starred_url":"https://api.github.com/users/hammer/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/hammer/subscriptions","organizations_url":"https://api.github.com/users/hammer/orgs","repos_url":"https://api.github.com/users/hammer/repos","events_url":"https://api.github.com/users/hammer/events{/privacy}","received_events_url":"https://api.github.com/users/hammer/received_events","type":"User","site_admin":false}],"milestone":null,"comments":54,"created_at":"2016-11-30T18:16:41Z","updated_at":"2016-12-14T16:21:42Z","closed_at":null,"body":"Some questions\r\n\r\n* Will FACS sorting disturb RNA?\r\n* How should we preserve the cells post-sorting and pre-RNA isolation?\r\n* How should we isolate RNA?\r\n* What markers should we sort on?\r\n"},"comment":{"url":"https://api.github.com/repos/hammerlab/immune-infiltrate-explorations/issues/comments/267079027","html_url":"https://github.com/hammerlab/immune-infiltrate-explorations/issues/14#issuecomment-267079027","issue_url":"https://api.github.com/repos/hammerlab/immune-infiltrate-explorations/issues/14","id":267079027,"user":{"login":"ChrystalPaulos","id":24443766,"avatar_url":"https://avatars.githubusercontent.com/u/24443766?v=3","gravatar_id":"","url":"https://api.github.com/users/ChrystalPaulos","html_url":"https://github.com/ChrystalPaulos","followers_url":"https://api.github.com/users/ChrystalPaulos/followers","following_url":"https://api.github.com/users/ChrystalPaulos/following{/other_user}","gists_url":"https://api.github.com/users/ChrystalPaulos/gists{/gist_id}","starred_url":"https://api.github.com/users/ChrystalPaulos/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ChrystalPaulos/subscriptions","organizations_url":"https://api.github.com/users/ChrystalPaulos/orgs","repos_url":"https://api.github.com/users/ChrystalPaulos/repos","events_url":"https://api.github.com/users/ChrystalPaulos/events{/privacy}","received_events_url":"https://api.github.com/users/ChrystalPaulos/received_events","type":"User","site_admin":false},"created_at":"2016-12-14T16:21:42Z","updated_at":"2016-12-14T16:21:42Z","body":"This 2009 paper by Mojgan, Rosenberg and crew gives a very different impression of PD-1 on the peripheral blood, tumor and normal tissue on T cells in cancer patients. Instead of 60% PD-1 it is maybe 6% PD-1 on CD8s. Not sure what to believe....\r\n\r\n[Mojgan PD-1 in blood, tumor and normal tissue.pdf](https://github.com/hammerlab/immune-infiltrate-explorations/files/652227/Mojgan.PD-1.in.blood.tumor.and.normal.tissue.pdf)\r\n\r\n"}}
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