{"action":"created","issue":{"url":"https://api.github.com/repos/samtools/hts-specs/issues/109","labels_url":"https://api.github.com/repos/samtools/hts-specs/issues/109/labels{/name}","comments_url":"https://api.github.com/repos/samtools/hts-specs/issues/109/comments","events_url":"https://api.github.com/repos/samtools/hts-specs/issues/109/events","html_url":"https://github.com/samtools/hts-specs/issues/109","id":117194333,"number":109,"title":"Suggestion: add RX and QX tags for molecular barcodes","user":{"login":"yfarjoun","id":2745554,"avatar_url":"https://avatars.githubusercontent.com/u/2745554?v=3","gravatar_id":"","url":"https://api.github.com/users/yfarjoun","html_url":"https://github.com/yfarjoun","followers_url":"https://api.github.com/users/yfarjoun/followers","following_url":"https://api.github.com/users/yfarjoun/following{/other_user}","gists_url":"https://api.github.com/users/yfarjoun/gists{/gist_id}","starred_url":"https://api.github.com/users/yfarjoun/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/yfarjoun/subscriptions","organizations_url":"https://api.github.com/users/yfarjoun/orgs","repos_url":"https://api.github.com/users/yfarjoun/repos","events_url":"https://api.github.com/users/yfarjoun/events{/privacy}","received_events_url":"https://api.github.com/users/yfarjoun/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"milestone":null,"comments":32,"created_at":"2015-11-16T18:59:24Z","updated_at":"2016-01-12T03:08:16Z","closed_at":null,"body":"I'd like to add standard tags RX and QX (to the SAM spec) for the bases of a molecular barcode and their qualities as read by the sequencer. \r\n\r\nTo be clear, this is *not* the sample barcode which can be used to demultiplex the sequence data, but rather a molecular barcode/index that can be used to identify the original molecule from which the sequence was generated. This can be used to disambiguate PCR from \"Biological\" duplicates (say in RNA-seq or other high depth technologies), or to identify the haplotype from whence many read pairs arose (as in 10X technology, e.g.)\r\n\r\nThere might be a need for other tags for the \"corrected\" barcode that is identified after comparing with a whitelist, but I'm not including that in this suggestion.\r\n\r\nSome discussion of this proposal has started here:  (https://github.com/broadinstitute/picard/issues/287)  \r\n\r\nI'd like to here what people think before submitting a PR here."},"comment":{"url":"https://api.github.com/repos/samtools/hts-specs/issues/comments/170769969","html_url":"https://github.com/samtools/hts-specs/issues/109#issuecomment-170769969","issue_url":"https://api.github.com/repos/samtools/hts-specs/issues/109","id":170769969,"user":{"login":"yfarjoun","id":2745554,"avatar_url":"https://avatars.githubusercontent.com/u/2745554?v=3","gravatar_id":"","url":"https://api.github.com/users/yfarjoun","html_url":"https://github.com/yfarjoun","followers_url":"https://api.github.com/users/yfarjoun/followers","following_url":"https://api.github.com/users/yfarjoun/following{/other_user}","gists_url":"https://api.github.com/users/yfarjoun/gists{/gist_id}","starred_url":"https://api.github.com/users/yfarjoun/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/yfarjoun/subscriptions","organizations_url":"https://api.github.com/users/yfarjoun/orgs","repos_url":"https://api.github.com/users/yfarjoun/repos","events_url":"https://api.github.com/users/yfarjoun/events{/privacy}","received_events_url":"https://api.github.com/users/yfarjoun/received_events","type":"User","site_admin":false},"created_at":"2016-01-12T03:08:16Z","updated_at":"2016-01-12T03:08:16Z","body":"Please note that I've put in a PR that specifies parts of this issue. please comment there (#119) if you have an opinion. "}}