{"action":"created","issue":{"url":"https://api.github.com/repos/rajewsky-lab/polyA/issues/66","repository_url":"https://api.github.com/repos/rajewsky-lab/polyA","labels_url":"https://api.github.com/repos/rajewsky-lab/polyA/issues/66/labels{/name}","comments_url":"https://api.github.com/repos/rajewsky-lab/polyA/issues/66/comments","events_url":"https://api.github.com/repos/rajewsky-lab/polyA/issues/66/events","html_url":"https://github.com/rajewsky-lab/polyA/issues/66","id":160586781,"number":66,"title":"shift in estimations from simulated data","user":{"login":"mschilli87","id":12913701,"avatar_url":"https://avatars.githubusercontent.com/u/12913701?v=3","gravatar_id":"","url":"https://api.github.com/users/mschilli87","html_url":"https://github.com/mschilli87","followers_url":"https://api.github.com/users/mschilli87/followers","following_url":"https://api.github.com/users/mschilli87/following{/other_user}","gists_url":"https://api.github.com/users/mschilli87/gists{/gist_id}","starred_url":"https://api.github.com/users/mschilli87/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mschilli87/subscriptions","organizations_url":"https://api.github.com/users/mschilli87/orgs","repos_url":"https://api.github.com/users/mschilli87/repos","events_url":"https://api.github.com/users/mschilli87/events{/privacy}","received_events_url":"https://api.github.com/users/mschilli87/received_events","type":"User","site_admin":false},"labels":[{"url":"https://api.github.com/repos/rajewsky-lab/polyA/labels/high%20priority","name":"high priority","color":"f9d0c4"}],"state":"open","locked":false,"assignee":{"login":"mschilli87","id":12913701,"avatar_url":"https://avatars.githubusercontent.com/u/12913701?v=3","gravatar_id":"","url":"https://api.github.com/users/mschilli87","html_url":"https://github.com/mschilli87","followers_url":"https://api.github.com/users/mschilli87/followers","following_url":"https://api.github.com/users/mschilli87/following{/other_user}","gists_url":"https://api.github.com/users/mschilli87/gists{/gist_id}","starred_url":"https://api.github.com/users/mschilli87/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mschilli87/subscriptions","organizations_url":"https://api.github.com/users/mschilli87/orgs","repos_url":"https://api.github.com/users/mschilli87/repos","events_url":"https://api.github.com/users/mschilli87/events{/privacy}","received_events_url":"https://api.github.com/users/mschilli87/received_events","type":"User","site_admin":false},"assignees":[{"login":"mschilli87","id":12913701,"avatar_url":"https://avatars.githubusercontent.com/u/12913701?v=3","gravatar_id":"","url":"https://api.github.com/users/mschilli87","html_url":"https://github.com/mschilli87","followers_url":"https://api.github.com/users/mschilli87/followers","following_url":"https://api.github.com/users/mschilli87/following{/other_user}","gists_url":"https://api.github.com/users/mschilli87/gists{/gist_id}","starred_url":"https://api.github.com/users/mschilli87/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mschilli87/subscriptions","organizations_url":"https://api.github.com/users/mschilli87/orgs","repos_url":"https://api.github.com/users/mschilli87/repos","events_url":"https://api.github.com/users/mschilli87/events{/privacy}","received_events_url":"https://api.github.com/users/mschilli87/received_events","type":"User","site_admin":false}],"milestone":null,"comments":31,"created_at":"2016-06-16T06:28:08Z","updated_at":"2016-07-14T10:57:41Z","closed_at":null,"body":"see https://github.com/rajewsky-lab/polyA/pull/63#issue-160419099:\r\n---\r\n - Simulating 400nt fragments only with poly(A) tail length of `42` results in expected value of `42` for estimated poly(A) tail length.\r\n - Simulating 90% 400nt fragments and 10% 399nt fragments with poly(A) tail length of `42` results in shift of the expected value for estimated poly(A) tail length to `43`.\r\n\r\nAny ideas why that could be?\r\n\r\n---\r\n\r\nNote that currently in the simulations all oligo-d(T)s anchor in the 3' end of the poly(A)-tail while the model assumes a uniform distribution across the whole poly(A)-tail.\r\n\r\nCould this be related?"},"comment":{"url":"https://api.github.com/repos/rajewsky-lab/polyA/issues/comments/232632815","html_url":"https://github.com/rajewsky-lab/polyA/issues/66#issuecomment-232632815","issue_url":"https://api.github.com/repos/rajewsky-lab/polyA/issues/66","id":232632815,"user":{"login":"mschilli87","id":12913701,"avatar_url":"https://avatars.githubusercontent.com/u/12913701?v=3","gravatar_id":"","url":"https://api.github.com/users/mschilli87","html_url":"https://github.com/mschilli87","followers_url":"https://api.github.com/users/mschilli87/followers","following_url":"https://api.github.com/users/mschilli87/following{/other_user}","gists_url":"https://api.github.com/users/mschilli87/gists{/gist_id}","starred_url":"https://api.github.com/users/mschilli87/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mschilli87/subscriptions","organizations_url":"https://api.github.com/users/mschilli87/orgs","repos_url":"https://api.github.com/users/mschilli87/repos","events_url":"https://api.github.com/users/mschilli87/events{/privacy}","received_events_url":"https://api.github.com/users/mschilli87/received_events","type":"User","site_admin":false},"created_at":"2016-07-14T10:57:41Z","updated_at":"2016-07-14T10:57:41Z","body":"Switching to `eps = 10e-20` resulted in 40s & 50s everywhere again."}}
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