{"action":"opened","issue":{"url":"https://api.github.com/repos/DerrickWood/kraken/issues/52","repository_url":"https://api.github.com/repos/DerrickWood/kraken","labels_url":"https://api.github.com/repos/DerrickWood/kraken/issues/52/labels{/name}","comments_url":"https://api.github.com/repos/DerrickWood/kraken/issues/52/comments","events_url":"https://api.github.com/repos/DerrickWood/kraken/issues/52/events","html_url":"https://github.com/DerrickWood/kraken/issues/52","id":173823881,"number":52,"title":"paired and unpaired reads","user":{"login":"helloworld44","id":9846408,"avatar_url":"https://avatars.githubusercontent.com/u/9846408?v=3","gravatar_id":"","url":"https://api.github.com/users/helloworld44","html_url":"https://github.com/helloworld44","followers_url":"https://api.github.com/users/helloworld44/followers","following_url":"https://api.github.com/users/helloworld44/following{/other_user}","gists_url":"https://api.github.com/users/helloworld44/gists{/gist_id}","starred_url":"https://api.github.com/users/helloworld44/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/helloworld44/subscriptions","organizations_url":"https://api.github.com/users/helloworld44/orgs","repos_url":"https://api.github.com/users/helloworld44/repos","events_url":"https://api.github.com/users/helloworld44/events{/privacy}","received_events_url":"https://api.github.com/users/helloworld44/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2016-08-29T17:13:23Z","updated_at":"2016-08-29T17:13:23Z","closed_at":null,"body":"Hi,\r\nI want to analyze a set of reads with kraken.\r\nAfter the trimming step of my paired-end data, a part of the reads lost their mates (mate was too short after quality trimming). I obtain a fastq file with the right mate, a fastq file with the left mate and a fastq file with singletons.\r\nPlease, could you tell me if is it possible to include all the reads (paired and singleton) in the kraken analysis ? Should I concatenate the files containing right/left paired reads with the file containing unpaired right/left mate or should I run kraken separately for the paired and unpaired reads ?\r\n\r\nThank you in advance for your reply,"}}
{"actor":{"display_login":"helloworld44"}}