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To start with, I will try seeding ~140K cells per well with the expectation, based on previous experiments with 12-well plates, that the cell count will quadruple in one day to 560K. ~80% confluence for a 12-well plate represented about 4.5 million cells per well, and we are estimating the area of a 96-well plate well to be 1/8 that of a 12-well plate well.\n+\n+### Transfection\n+\n+| Reagent                   | 12-well Volume (ul/well) | 96-well Volume (ul/well) | Dilution |\n+|---------------------------|--------------------------|--------------------------|----------|\n+| Plasmids                  | 1                        | 1                        | 1:8      |\n+| PHH                       | 1.5                      | 1.5                      | 1:8      |\n+| pCD                       | 0.67                     | 0.67                     | 1:8      |\n+| OPTIMEM + Lipofectamine MM | 252.5                    | 31.56                    | 1:1      |","path":"experiments/20161104-pol-assay-high-throughput/20161104-first-attempt.md","position":18,"original_position":18,"commit_id":"808d984c722637a49f8b161f8e75937d81c2b33e","original_commit_id":"808d984c722637a49f8b161f8e75937d81c2b33e","user":{"login":"ericmjl","id":2631566,"avatar_url":"https://avatars.githubusercontent.com/u/2631566?v=3","gravatar_id":"","url":"https://api.github.com/users/ericmjl","html_url":"https://github.com/ericmjl","followers_url":"https://api.github.com/users/ericmjl/followers","following_url":"https://api.github.com/users/ericmjl/following{/other_user}","gists_url":"https://api.github.com/users/ericmjl/gists{/gist_id}","starred_url":"https://api.github.com/users/ericmjl/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ericmjl/subscriptions","organizations_url":"https://api.github.com/users/ericmjl/orgs","repos_url":"https://api.github.com/users/ericmjl/repos","events_url":"https://api.github.com/users/ericmjl/events{/privacy}","received_events_url":"https://api.github.com/users/ericmjl/received_events","type":"User","site_admin":false},"body":"May want to note after the table that the concentrations in the 96-well plate volumes will result in reagents 8x as concentrated as they are in the 12-well plate. 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