{"action":"created","issue":{"url":"https://api.github.com/repos/pombase/fypo/issues/2896","repository_url":"https://api.github.com/repos/pombase/fypo","labels_url":"https://api.github.com/repos/pombase/fypo/issues/2896/labels{/name}","comments_url":"https://api.github.com/repos/pombase/fypo/issues/2896/comments","events_url":"https://api.github.com/repos/pombase/fypo/issues/2896/events","html_url":"https://github.com/pombase/fypo/issues/2896","id":191352943,"number":2896,"title":"ntr:normal movement along microtubules","user":{"login":"ValWood","id":7359272,"avatar_url":"https://avatars.githubusercontent.com/u/7359272?v=3","gravatar_id":"","url":"https://api.github.com/users/ValWood","html_url":"https://github.com/ValWood","followers_url":"https://api.github.com/users/ValWood/followers","following_url":"https://api.github.com/users/ValWood/following{/other_user}","gists_url":"https://api.github.com/users/ValWood/gists{/gist_id}","starred_url":"https://api.github.com/users/ValWood/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ValWood/subscriptions","organizations_url":"https://api.github.com/users/ValWood/orgs","repos_url":"https://api.github.com/users/ValWood/repos","events_url":"https://api.github.com/users/ValWood/events{/privacy}","received_events_url":"https://api.github.com/users/ValWood/received_events","type":"User","site_admin":false},"labels":[{"id":232758130,"url":"https://api.github.com/repos/pombase/fypo/labels/community%20curation","name":"community curation","color":"c7def8","default":false}],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":14,"created_at":"2016-11-23T18:47:53Z","updated_at":"2016-11-28T10:32:59Z","closed_at":null,"body":"https://curation.pombase.org/pombe/curs/f2b0ffda72370b22/ro\r\n\r\nnormal protein transport on microtubule tip\r\nnormal protein transport by microtubule polymerization\r\nsituation where the rate of protein transport on a polymerising microtubule tip is the same as wild type\r\n\r\nhttps://github.com/geneontology/go-ontology/issues/12822"},"comment":{"url":"https://api.github.com/repos/pombase/fypo/issues/comments/263237097","html_url":"https://github.com/pombase/fypo/issues/2896#issuecomment-263237097","issue_url":"https://api.github.com/repos/pombase/fypo/issues/2896","id":263237097,"user":{"login":"JackyVH","id":13981032,"avatar_url":"https://avatars.githubusercontent.com/u/13981032?v=3","gravatar_id":"","url":"https://api.github.com/users/JackyVH","html_url":"https://github.com/JackyVH","followers_url":"https://api.github.com/users/JackyVH/followers","following_url":"https://api.github.com/users/JackyVH/following{/other_user}","gists_url":"https://api.github.com/users/JackyVH/gists{/gist_id}","starred_url":"https://api.github.com/users/JackyVH/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/JackyVH/subscriptions","organizations_url":"https://api.github.com/users/JackyVH/orgs","repos_url":"https://api.github.com/users/JackyVH/repos","events_url":"https://api.github.com/users/JackyVH/events{/privacy}","received_events_url":"https://api.github.com/users/JackyVH/received_events","type":"User","site_admin":false},"created_at":"2016-11-28T10:32:59Z","updated_at":"2016-11-28T10:32:59Z","body":"Hi Val\n\nIs there any evidence that makes transport of tea1 actually on the tip by polymerization (as opposed to tea2 keeping it at the tip by its motor activity) a possibility.\n\n\n\nIn PMID1203477, they show that the rate of tea1GFP movement on microtubules is the same in wild type and tea2 Delta strains suggesting tea2 is not moving tea1GFP Fig2B, and they also show that tea1GFP co-localises with the growing microtubule ends.  I think the  cell tip localisation is indirect and the accumulation of tea1 in the vicinity of the nucleus is indirect because tea2  affects the length of the microtubules.  Does this help I'll also have a look at PMID 11018050\n\nJacky\n\nFrom: Val Wood <notifications@github.com<mailto:notifications@github.com>>\nReply-To: pombase/fypo <reply@reply.github.com<mailto:reply@reply.github.com>>\nDate: Friday, 25 November 2016 at 11:40\nTo: pombase/fypo <fypo@noreply.github.com<mailto:fypo@noreply.github.com>>\nCc: \"Jacqueline.Hayles@crick.ac.uk<mailto:Jacqueline.Hayles@crick.ac.uk>\" <Jacqueline.Hayles@crick.ac.uk<mailto:Jacqueline.Hayles@crick.ac.uk>>, Comment <comment@noreply.github.com<mailto:comment@noreply.github.com>>\nSubject: Re: [pombase/fypo] ntr:normal movement along microtubules (#2896)\n\n\nHi @jvhayles\n\nI had another look at these papers:\n\nConclusions of PMID:11018050\nIn the absence of Tea2p, Tea1p localizes along the short cytoplasmic microtubules characteristic of tea2delta cells. Therefore, although Tea1p has an affinity for microtubules in the absence of Tea2p, proper localization of Tea1p to the cell tip requires Tea2p. One possibility is that Tea2p transports Tea1p along microtubules and deposits it at the cell tip. A second possibility is that Tea1p uses an- other microtubule-mediated mechanism to get to the tip of the cell, and the absence of a normal array of cytoplasmic microtubules in tea2delta cells results in the mislocalization of Tea1p.\n\nSo I don't think this paper says that tea1 is transported on the tip.\nIt says\nTea2 is not required for localization to microtubules\nTea2 is required for localization to the tip.\nIt doesn't say anything about the mechanism from this paper, and probably stick with the localization phenotypes?\n\nThe later paper on tea2/tip1 appears to have similar phenotypes to the Tea2/tea1\nbut also looks at movement along microtubule and concludes that\nTea2 transports tip1 to the tip.\n\nIs there any evidence that makes transport of tea1 actually on the tip by polymerization (as opposed to tea2 keeping it at the tip by its motor activity) a possibility.\n\nOtherwise, we might have created a GO term for something which is not mechanistically possible....\n\n-\nYou are receiving this because you commented.\nReply to this email directly, view it on GitHub<https://github.com/pombase/fypo/issues/2896#issuecomment-262939114>, or mute the thread<https://github.com/notifications/unsubscribe-auth/ANVVaMle_mIo1SHReJg_XkMw_pHbpiOcks5rBskbgaJpZM4K67ju>.\n\nThe Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 215 Euston Road, London NW1 2BE.\n"}}
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