{"action":"created","issue":{"url":"https://api.github.com/repos/geneontology/go-ontology/issues/12431","repository_url":"https://api.github.com/repos/geneontology/go-ontology","labels_url":"https://api.github.com/repos/geneontology/go-ontology/issues/12431/labels{/name}","comments_url":"https://api.github.com/repos/geneontology/go-ontology/issues/12431/comments","events_url":"https://api.github.com/repos/geneontology/go-ontology/issues/12431/events","html_url":"https://github.com/geneontology/go-ontology/issues/12431","id":151635192,"number":12431,"title":"mitotic spindle elongation (again)","user":{"login":"ValWood","id":7359272,"avatar_url":"https://avatars.githubusercontent.com/u/7359272?v=3","gravatar_id":"","url":"https://api.github.com/users/ValWood","html_url":"https://github.com/ValWood","followers_url":"https://api.github.com/users/ValWood/followers","following_url":"https://api.github.com/users/ValWood/following{/other_user}","gists_url":"https://api.github.com/users/ValWood/gists{/gist_id}","starred_url":"https://api.github.com/users/ValWood/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ValWood/subscriptions","organizations_url":"https://api.github.com/users/ValWood/orgs","repos_url":"https://api.github.com/users/ValWood/repos","events_url":"https://api.github.com/users/ValWood/events{/privacy}","received_events_url":"https://api.github.com/users/ValWood/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":{"login":"ukemi","id":11067494,"avatar_url":"https://avatars.githubusercontent.com/u/11067494?v=3","gravatar_id":"","url":"https://api.github.com/users/ukemi","html_url":"https://github.com/ukemi","followers_url":"https://api.github.com/users/ukemi/followers","following_url":"https://api.github.com/users/ukemi/following{/other_user}","gists_url":"https://api.github.com/users/ukemi/gists{/gist_id}","starred_url":"https://api.github.com/users/ukemi/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ukemi/subscriptions","organizations_url":"https://api.github.com/users/ukemi/orgs","repos_url":"https://api.github.com/users/ukemi/repos","events_url":"https://api.github.com/users/ukemi/events{/privacy}","received_events_url":"https://api.github.com/users/ukemi/received_events","type":"User","site_admin":false},"milestone":null,"comments":1,"created_at":"2016-04-28T12:59:58Z","updated_at":"2016-04-29T10:25:10Z","closed_at":null,"body":"\r\nSorry David ! I think we can improve this.\r\nGO:0000022 mitotic spindle elongation\r\nThe cell cycle process in which the distance is lengthened between poles of the mitotic spindle. Mitotic spindle elongation begins during mitotic prophase and ends during mitotic anaphase B.\r\n\r\nThis paper is useful: PMID:9802907 (I should have done this one earlier)\r\n\r\nAbstract\r\nIn higher eukaryotic cells, the spindle forms along with chromosome condensation in mitotic prophase. In metaphase, chromosomes are aligned on the spindle with sister kinetochores facing toward the opposite poles. In anaphase A, sister chromatids separate from each other without spindle extension, whereas spindle elongation takes place during anaphase B. \r\n\r\nIntro\r\nearly mitotic to late anaphase events were observed in living fission yeast cells. S. pombe has three phases in spindle dynamics, spindle formation (phase 1), constant spindle length (phase 2), and spindle extension (phase 3). Sister centromere separation (anaphase A) rapidly occurred at the end of phase 2. The centromere showed dynamic movements throughout phase 2 as it moved back and forth and was transiently split in two before its separation\r\nDur- ing the early stages of mitosis, the SPBs separate from each other, the spindle forms between them, and cy- toplasmic microtubules disappear (e.g., Masuda et al., 1992). The interphase centromeres are known to be clustered and located near the SPB (Takahashi et al., 1992; Funabiki et al., 1993). In situ hybridization with the centromere DNA indicated that this interphase centromere clustering was disrupted on entry into mitosis, and that the individual centromeres appeared to interact with the short spindle (Funabiki et al., 1993; Saitoh et al., 1997).\r\n\r\nPhase 1 corre- sponded to the period of spindle formation (e.g., a prophase-like stage), in which the spindle length in- creased from 0 to 2.5 􏰈m; phase 2 represented the period of constant spindle length (e.g., metaphase–anaphase A); and phase 3 was the period of spindle extension from 2.5 to 12–15 􏰈m (anaphase B). These three distinct phases were found in all the wild-type cells examined, although the duration of each phase was strongly dependent on the temperature used (Figure 2, B–E).\r\n\r\n\r\nSo what this says is  the \"elongation\" which occurs during metaphase is actually \"(mitotic) spindle assembly\"\r\nand the  \"rapid elongation\" that occurs in anaphase B is \"(mitotic) spindle extension\"\r\n \r\nThis seems to hold from yeast to human, so I wonder if we should disambiguate like this  and have \"(mitotic) spindle  elongation as a broad synonym for both terms?\r\n\r\nWe need to separate because the 2  processes are quite different and at present there is a danger of lumping the annotation....\r\n\r\n\r\n"},"comment":{"url":"https://api.github.com/repos/geneontology/go-ontology/issues/comments/215678985","html_url":"https://github.com/geneontology/go-ontology/issues/12431#issuecomment-215678985","issue_url":"https://api.github.com/repos/geneontology/go-ontology/issues/12431","id":215678985,"user":{"login":"mah11","id":5196259,"avatar_url":"https://avatars.githubusercontent.com/u/5196259?v=3","gravatar_id":"","url":"https://api.github.com/users/mah11","html_url":"https://github.com/mah11","followers_url":"https://api.github.com/users/mah11/followers","following_url":"https://api.github.com/users/mah11/following{/other_user}","gists_url":"https://api.github.com/users/mah11/gists{/gist_id}","starred_url":"https://api.github.com/users/mah11/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mah11/subscriptions","organizations_url":"https://api.github.com/users/mah11/orgs","repos_url":"https://api.github.com/users/mah11/repos","events_url":"https://api.github.com/users/mah11/events{/privacy}","received_events_url":"https://api.github.com/users/mah11/received_events","type":"User","site_admin":false},"created_at":"2016-04-29T10:25:10Z","updated_at":"2016-04-29T10:25:10Z","body":"The spindle can get longer (defined as pole-to-pole distance) either microtubule polymerization or by midzone MTs sliding along each other to push the poles apart (thereby making the area of overlapping, interdigitated polar MTs smaller). So, questions:\r\n\r\n1) Is MT polymerization always \"spindle assembly\"? If not, where does assembly stop and elongation of an existing spindle by MT polymerization begin? This may depend on whether any MT polymerization takes place in \"phase 3\".\r\n\r\n2) Is the requested \"spindle extension\" identical to the MT-based movement that pushes the poles apart? \r\n\r\nI don't know the answers, but it looks key to defining the requested terms.\r\n\r\nIncidentally, I don't think using the word \"extension\" adds any distinction - in plain English it actually has a somewhat broader meaning than \"elongation\". If MT polymerization is always assembly in the spindle context, then we can use \"elongation\" for the movement-based part; if there is polymerization-mediated elongation, then we'll need long \"elongation by x\" terms. (Sorry.)"}}