immunodiffusion immunodiffusion immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen. immunodiffusionmeshd005779 the commonly known types are: single diffusion in one dimension (oudin procedure) double diffusion in one dimension (oakley fulthorpe procedure) single diffusion in two dimensions (radial immunodiffusion or mancini method) double diffusion in two dimensions (ouchterlony double immunodiffusion) notes coleman, r. m.; kaufman, l. (1972). "use of the immunodiffusion test in the serodiagnosis of aspergillosis". applied microbiology. 23 (2): 301–308. doi:10.1128/am.23.2.301-308.1972. pmc 380335. pmid 4622826. "radial immunodiffusion". edvotek, inc. 2017. archived from the original (photograph) on 2017-08-07. retrieved 2017-08-07. photograph of precipitin circles in a petri dish during radial immunodiffusion. "diffusion patterns". immunodiffusion principles and application. archived from the original on 2019-12-11. retrieved 2017-05-19. photographs of ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non-identity.