list of coronavirus live isolates list of coronavirus live isolates this article aims at keeping an up-to-date list of coronavirus strains and subspecies successfully isolated and cultured in laboratory, a task which is often challenging. when relevant it shall include a few synthetic chimera as well as some strains that were only propagated in laboratory animals. alphacoronavirus 229e nl63 pedv (adding trypsin to vero cells), propagated in piglets, repeated passage in vero cells, using vero/tmprss2 and vero/mspl cells sads tegacovirus tgev prcv feline coronavirus canine coronavirus. a recent recombinant ccov-hupn-2018 including a human case betacoronavirus merbecovirus hku5 sars-s chimera hku5 and a mers pdf-2180-s chimera, with trypsin various mers hku4-rbd chimera hku4 mers embecovirus bovine coronavirus rabbit hku14 mhv oc43 (1967) hku1 parker rat coronavirus dromedary camel hku23 canine respiratory coronavirus (crcov) equine coronavirus sarbecovirus sars hku3 sars-rbd synthetic recombinant bat-sl-cov zc45 (in suckling rats) wiv1 rsshc014 (synthetic clone) wiv16 wiv1 rs4874-s synthetic chimera rs4874 and two synthetic chimera wiv1 rs7237-s and wiv1 rs4231-s sars-cov-2 banal-236 pangolin guangxi p2v rco319, rc-os20, rc-mk2, rc-kw8 (in vero with rc-ace2) wiv1 rs4081-s synthetic chimera (with trypsin). in the spike it is a member of clade 2 (including hku3). wiv1 rsshc014-s1 chimera two clade 1 viruses (using ace2) rsyn2012 and rsyn2016 and one clade 2 virus (not using ace2) rshub2019 in huh7 cells with trypsin. gammacoronavirus avian coronavirus (ibv) turkey coronavirus deltacoronavirus porcine deltacoronavirus hku15 including a human case parrot coronavirus