protamine protamine protamines are small, arginine-rich, nuclear proteins that replace histones late in the haploid phase of spermatogenesis and are believed essential for sperm head condensation and dna stabilization. they may allow for denser packaging of dna in the spermatozoon than histones, but they must be decompressed before the genetic data can be used for protein synthesis. however, in humans and maybe other primates, 10-15% of the sperm's genome is packaged by histones thought to bind genes that are essential for early embryonic development. protamine 1identifierssymbolprm1ncbi gene5619hgnc9447omim182880refseqnm_002761uniprotp04553other datalocuschr. 16 p13.13search forstructuresswiss-modeldomainsinterpro protamine 2identifierssymbolprm2ncbi gene5620hgnc9448omim182890refseqnm_002762uniprotp04554other datalocuschr. 16 p13.13search forstructuresswiss-modeldomainsinterpro protamine and protamine-like (pl) proteins are collectively known as the sperm-specific nuclear basic proteins (snbps). the pl proteins are intermediate in structure between protamine and histone h1. the c-terminal domain of pl could be the precursor of vertebrate protamine. spermatogenesis alterations to the epigenome post-fertilization. the upper part of the image shows replacement of protamines with histones in paternal pronucleus shortly after fertilization. dna packaged with protamines forms toroid-shaped structures, shown at the top left corner of the image.during the formation of sperm, protamine binds to the phosphate backbone of dna using the arginine-rich domain as an anchor. dna is then folded into a toroid, an o-shaped structure, although the mechanism is not known. a sperm cell can contain up to 50,000 toroid-shaped structures in its nucleus with each toroid containing about 50 kilobases. before the toroid is formed, histones are removed from the dna by transition nuclear proteins, so that protamine can condense it. the effects of this change are 1) an increase in sperm hydrodynamics for better flow through liquids by reducing the head size 2) decrease in the occurrence of dna damage 3) removal of the epigenetic markers that occur with histone modifications. the structure of the sperm head is also related to protamine levels. the ratio of protamine 2 to protamine 1 and transition nuclear proteins has been found to change the sperm head shape in various species of mice, by altering the expression of protamine 2 via mutations in its promoter region. a decrease in the ratio has been found to increase the competitive ability of sperm in mus species. however, further testing is required to determine how this ratio influences the shape of the head and whether monogamy influences this selection. in humans, studies show that men who have unbalanced prm1/prm2 are subfertile or infertile. protamine 2 is encoded as a longer protein that needs its n-terminal cleaved before becoming functional. human and chimp protamine has undergone rapid evolution. medical uses