20α,22r-dihydroxycholesterol 20α,22r-dihydroxycholesterol 20α,22r-dihydroxycholesterol, or (3β)-cholest-5-ene-3,20,22-triol is an endogenous, metabolic intermediate in the biosynthesis of the steroid hormones from cholesterol. cholesterol ((3β)-cholest-5-en-3-ol) is hydroxylated by cholesterol side-chain cleavage enzyme (p450scc) to form 22r-hydroxycholesterol, which is subsequently hydroxylated again by p450scc to form 20α,22r-dihydroxycholesterol, and finally the bond between carbons 20 and 22 is cleaved by p450scc to form pregnenolone ((3β)-3-hydroxypregn-5-en-20-one), the precursor to the steroid hormones. 20α,22r-dihydroxycholesterol names iupac name (22r)-cholest-5-ene-3β,20,22-triol systematic iupac name (2r,3r)-2-phenanthren-1-yl]-6-methylheptane-2,3-diol identifiers cas number 596-94-1 n 3d model (jsmol) interactive image beilstein reference 2339391 chebi chebi:1294 chembl chembl560194 chemspider 4956189 kegg c05501 pubchem cid 6453841 comptox dashboard (epa) dtxsid00975007 inchi inchi=1s/c27h46o3/c1-17(2)6-11-24(29)27(5,30)23-10-9-21-20-8-7-18-16-19(28)12-14-25(18,3)22(20)13-15-26(21,23)4/h7,17,19-24,28-30h,6,8-16h2,1-5h3/t19-,20-,21-,22-,23-,24+,25-,26-,27+/m0/s1key: isbssbgeyibvto-tykwndpbsa-n smiles cc(c)cc((c)(1cc21(cc32cc=c43(cc(c4)o)c)c)o)o properties chemical formula c27h46o3 molar mass 418.652 g/mol except where otherwise noted, data are given for materials in their standard state (at 25 °c , 100 kpa). infobox references