urovysion urovysion urovysion is a fluorescence in situ hybridization assay that was developed for the detection of bladder cancer in urine specimens. it consists of fluorescently labeled dna probes to the pericentromeric regions of chromosomes 3 (red), 7 (green), and 17 (aqua) and to the 9p21 band (gold) location of the p16 tumor suppressor gene.