mixed lineage kinase domain like pseudokinase mixed lineage kinase domain like pseudokinase mixed lineage kinase domain like pseudokinase (mlkl) is a protein that in humans is encoded by the mlkl gene. mlklavailable structurespdbortholog search: pdbe rcsb list of pdb id codes2msv, 4m67, 4mwiidentifiersaliasesmlkl, hmixed lineage kinase domain-like, mixed lineage kinase domain like pseudokinaseexternal idsomim: 615153 mgi: 1921818 homologene: 77416 genecards: mlkl gene location (human)chr.chromosome 16 (human)band16q23.1start74,671,855 bpend74,700,960 bpgene location (mouse)chr.chromosome 8 (mouse)band8|8 e1start112,038,429 bpend112,064,809 bprna expression patternbgeehumanmouse (ortholog)top expressed inmonocytebloodspleenupper lobe of left lungappendixright lungpancreatic epithelial cellbone marrowbone marrow cellscancellous bonetop expressed inbone marrowepithelium of stomachduodenumpaneth cellsubcutaneous adipose tissuecolonjejunumwhite adipose tissuecervixleft colonmore reference expression databiogpsn/agene ontologymolecular function nucleotide binding protein binding atp binding protein kinase binding protein kinase activity identical protein binding signal transducer activity protein-containing complex binding protein serine/threonine kinase activity jun kinase kinase kinase activity cellular component cytoplasm cytosol plasma membrane membrane biological process necroptosis protein homotrimerization programmed cell death protein phosphorylation intracellular signal transduction cell surface receptor signaling pathway mapk cascade sources:amigo / quickgoorthologsspecieshumanmouseentrez19725974568ensemblensg00000168404ensmusg00000012519uniprotq8nb16q9d2y4refseq (mrna)nm_001142497nm_152649nm_029005nm_001310613refseq (protein)np_001135969np_689862np_001297542np_083281location (ucsc)chr 16: 74.67 – 74.7 mbchr 8: 112.04 – 112.06 mbpubmed searchwikidataview/edit humanview/edit mouse function this gene belongs to the protein kinase superfamily. the encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. this protein plays a critical role in tumor necrosis factor (tnf)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (rip3), which is a key signaling molecule in necroptosis pathway. inhibitor studies and knockdown of this gene inhibited tnf-induced necrosis. influence in diseases high levels of this protein and rip3 are associated with inflammatory bowel disease in children. alternatively spliced transcript variants have been described for this gene. . some reports have linked it to unusual variants of multiple sclerosis