natrialbales natrialbales natrialbales is an order of halophilic, chemoorganotrophic archaea within the class haloarchaea. the type genus of this order is natrialba. natrialbales scientific classification domain: archaea kingdom: euryarchaeota class: halobacteria order: natrialbalesgupta et al. 2015 genus natrialbaceae halobiforma halopiger halostagnicola haloterrigena halovivax natrarchaeobaculum natrarchaeobius natrialba natrinema natronobacterium natronobiforma natronococcus natronolimnobius natronolimnohabitans natronorubrum salinadaptatus salinarchaeum saliphagus the name natrialbales is derived from the latin term natriabla, referring to the type genus of the order and the suffix "-ales", an ending used to denote an order. together, natrialbales refers to an order whose nomenclatural type is the genus natrialba. biochemical characteristics and molecular signatures members are halophilic chemoorganotrophs and are mainly isolated from high-salt environments such as saline lakes, soda lakes and salted hides. some members are motile. morphology is variable, including rod, coccus or pleomorphic shapes. majority of the class are able to grow optimally in alkaline ph and do not possess gas vesicles. the dna g+c content for this order ranges between 60-70 mol%. this order can be reliably distinguished from other orders within the phylum euryarchaeota by the presence of eight conserved signature proteins (csps) and two conserved signature indels (csis) present in the ribosomal operon protein and small gtp-binding protein. historical systematics and taxonomy as of 2021, natrialbales contains one family, natrialbaceae. members of this order was demarcated from the class halobacteria, previously a large phylogenetically unrelated group of species with distinct biochemical characteristics and different ecological niches. 16s rrna based phylogenetic trees and morphological/physiological characteristics were not sufficient to clarify the evolutionary relationship above the genus level within the class halobacteria. in 2015, gupta et al. proposed the division of the class halobacteria into halobacteriales, haloferacales and natrialbales based on comparative genomic analyses and the branching pattern of various phylogenetic trees constructed from several different datasets of conserved proteins and 16s rrna sequences. molecular markers, specifically conserved signature indels, specific to this order were also identified as evidence supporting the division independent of phylogenetic trees. phylogeny the currently accepted taxonomy is based on the list of prokaryotic names with standing in nomenclature (lpsn) and national center for biotechnology information (ncbi). 16s rrna-based ltp_01_2022 53 marker proteins based gtdb 07-rs207 halorubellus * haloarchaeobius * natronoarchaeum * salinarchaeum natrialbaceae halovivax 2 natronobiforma sorokin et al. 2019 saliphagus yin et al. 2017 halovarius mehrshad et al. 2015 * natribaculum liu et al. 2015 * natronobacterium tindall et al. 1984 halobiforma hezayen et al. 2002 halovivax castillo et al. 2006 natronococcus tindall et al. 1984 natrarchaeobaculum sorokin et al. 2020 natronolimnohabitans sorokin et al. 2020 natronolimnobius itoh et al. 2005 salinadaptatus xue et al. 2020 halopiger gutierrez et al. 2007 natrarchaeobius halalkaliphilus natronorubrum xu et al. 1999 natrialba kamekura & dyall-smith 1996 (incl. natrarchaeobius) natrinema 2 halostagnicola castillo et al. 2006 haloterrigena 2 haloterrigena ventosa et al. 1999 natrinema mcgenity et al. 1998 natrialbaceae halorubellus haloarchaeobius halovivax saliphagus halostagnicola natronococcus halopiger goleimassiliensis natrarchaeobaculum salinadaptatus natrarchaeobius corrig. sorokin et al. 2020 halopiger halopiger djelfimassiliensis natronobacterium halobiforma natronolimnobius natrialba natronolimnohabitans haloterrigena natronorubrum natrinema note: * paraphyletic halobacteriaceae