conformation–activity relationship conformation–activity relationship in biochemistry, the conformation–activity relationship is the relationship between the biological activity and the chemical structure or conformational changes of a biomolecule. this terminology emphasizes the importance of dynamic conformational changes for the biological function, rather than the importance of static three-dimensional structure used in the analysis of structure–activity relationships. the conformational changes usually take place during intermolecular association, such as protein–protein interaction or protein–ligand binding. a binding partner changes the conformation of a biomolecule (e.g. a protein) to enable or disable its biochemical activity. methods for analysis of conformation activity relationship vary from in silico or using experimental methods such as x-ray crystallography and nmr where the conformation before and after activity can be compared statically or using dynamic methods such as multi-parametric surface plasmon resonance, dual-polarisation interferometry or circular dichroism where the kinetics as well as degree of conformational change can be quantified. experimental techniques static static experimental techniques include x-ray crystallography and nmr. dynamic dynamic experimental techniques include multi-parametric surface plasmon resonance, dual-polarization interferometry, and circular dichroism.