vesamicol vesamicol vesamicol is an experimental drug, acting presynaptically by inhibiting acetylcholine (ach) uptake into synaptic vesicles and reducing its release. vesamicol may have applications for the treatment of adenocarcinoma in situ of the lung. vesamicollegal statuslegal status investigational identifiers iupac name 2-(4-phenyl-1-piperidyl)cyclohexan-1-ol cas number22232-64-0 ypubchem cid5662iuphar/bps4759chemspider5460unii3d416v0flmchemblchembl20730comptox dashboard (epa)dtxsid701009898 chemical and physical dataformulac17h25nomolar mass259.393 g·mol−13d model (jsmol)interactive image smiles oc1ccccc1n2ccc(cc2)c3ccccc3 mechanism of action vesamicol can be broadly categorized as a cholinergic physiological antagonist, because it reduces the apparent activity of cholinergic neurons, but does not act at the postsynaptic ach receptor. vesamicol causes a non-competitive and reversible block of the intracellular transporter vacht responsible for carrying newly synthesized ach into secretory vesicles in the presynaptic nerve terminal. this transport process is driven by a proton gradient between cell organelles and the cytoplasm. blocking of acetylcholine loading leads to empty vesicles fusing with neuron membranes, decreasing ach release.