bleomycin bleomycin bleomycin is a medication used to treat cancer. this includes hodgkin's lymphoma, non-hodgkin's lymphoma, testicular cancer, ovarian cancer, and cervical cancer among others. typically used with other cancer medications, it can be given intravenously, by injection into a muscle or under the skin. it may also be administered inside the chest to help prevent the recurrence of a fluid around the lung due to cancer; however talc is better for this. bleomycinbleomycin a2clinical datatrade namesblenoxaneahfs/drugs.commonographmedlineplusa682125license data eu ema: by inn us dailymed: bleomycin us fda: bleomycin pregnancycategory au: d d routes ofadministrationintravenous, intramuscular, subcutaneous, intrapleuraldrug classglycopeptide antibioticatc codel01dc01 (who) legal statuslegal status uk: pom (prescription only) us: ℞-only pharmacokinetic databioavailability100% and 70% following intramuscular and subcutaneous administrations, respectively, and 45% following both intraperitoneal and intrapleural administrationselimination half-lifetwo hoursexcretionrenal (60–70%)identifiers iupac name (3--3-oxopropyl] -5-methylpyrimidin-4-yl}-13-oxy} -4,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2h-pyran-2-yl]oxy} (1h-imidazol-5-yl)methyl]-9-hydroxy-5--8,10-dimethyl-4,7,12,15-tetraoxo-3,6,11,14-tetraazapentadec-1-yl}-2,4'-bi-1,3-thiazol-4-yl)carbonyl]amino}propyl)(dimethyl)sulfonium cas number11056-06-7 ysulfate: 9041-93-4 ypubchem cid5460769drugbankdb00290 nchemspider4574226 nunii40s1vhn69bsulfate: 7dp3ntv15t ykeggd07535 nsulfate: c15773 nchebichebi:22907 nchemblchembl403664 ncomptox dashboard (epa)dtxsid1030862 chemical and physical dataformulac55h84n17o21s3molar mass1415.551 g·mol−13d model (jsmol)interactive image smiles cc1=c(n=c(n=c1n)(cc(=o)n)nc(c(=o)n)n)c(=o)n(c(c2=cn=cn2)o3((((o3)co)o)o)o4((((o4)co)o)oc(=o)n)o)c(=o)n(c)((c)c(=o)n((c)o)c(=o)nccc5=nc(=cs5)c6=nc(=cs6)c(=o)nccc(c)c)o inchi inchi=1s/c55h83n17o21s3/c1-20-33(69-46(72-44(20)58)25(12-31(57)76)64-13-24(56)45(59)82)50(86)71-35(41(26-14-61-19-65-26)91-54-43(39(80)37(78)29(15-73)90-54)92-53-40(81)42(93-55(60)88)38(79)30(16-74)89-53)51(87)66-22(3)36(77)21(2)47(83)70-34(23(4)75)49(85)63-10-8-32-67-28(18-94-32)52-68-27(17-95-52)48(84)62-9-7-11-96(5)6/h14,17-19,21-25,29-30,34-43,53-54,64,73-75,77-81h,7-13,15-16,56h2,1-6h3,(h13-,57,58,59,60,61,62,63,65,66,69,70,71,72,76,82,83,84,85,86,87,88)/p+1/t21-,22+,23+,24-,25-,29-,30+,34-,35-,36-,37+,38+,39-,40-,41-,42-,43-,53+,54-/m0/s1 nkey:oyvagsvqbohsss-uapagmarsa-o n ny (what is this?) (verify) common side effects include fever, weight loss, vomiting, and rash. a severe type of anaphylaxis may occur. it may also cause inflammation of the lungs that can result in lung scarring. chest x-rays every couple of weeks are recommended to check for this. bleomycin may cause harm to the baby if used during pregnancy. it is believed to primarily work by preventing the synthesis of dna. bleomycin was discovered in 1962. it is on the world health organization's list of essential medicines. it is available as a generic medication. it is made by the bacterium streptomyces verticillus. medical uses cancer bleomycin is mostly used to treat cancer. this includes testicular cancer, ovarian cancer, and hodgkin's disease, and less commonly non-hodgkin's disease. it can be given intravenously, by intramuscular injection, or under the skin. other uses it may also be put inside the chest to help prevent the recurrence of a pleural effusion due to cancer. however, for scarring down the pleura, talc appears to be the better option although indwelling pleural catheters are at least as effective in reducing the symptoms of an effusion(such as dyspnea). while potentially effective against bacterial infections, its toxicity prevents its use for this purpose. it has been studied in the treatment of warts but is of unclear benefit. side effects the most common side effects are flu-like symptoms and include fever, rash, dermatographism, hyperpigmentation, alopecia (hair loss), chills, and raynaud's phenomenon (discoloration of fingers and toes). the most serious complication of bleomycin, occurring upon increasing dosage, is pulmonary fibrosis and impaired lung function. it has been suggested that bleomycin induces sensitivity to oxygen toxicity and recent studies support the role of the proinflammatory cytokines il-18 and il-1beta in the mechanism of bleomycin-induced lung injury. any previous treatment with bleomycin should therefore always be disclosed to the anaesthetist prior to undergoing a procedure requiring general anaesthesia. due to the oxygen sensitive nature of bleomycin, and the theorised increased likelihood of developing pulmonary fibrosis following supplemental oxygen therapy, it has been questioned whether patients should take part in scuba diving following treatment with the drug. bleomycin has also been found to disrupt the sense of taste. lifetime cumulative dose bleomycin should not exceed a lifetime cumulative dose greater than 400 units. pulmonary toxicities, most commonly presenting as pulmonary fibrosis, are associated with doses of bleomycin greater than 400 units. mechanism of action bleomycin acts by induction of dna strand breaks. some studies suggest bleomycin also inhibits incorporation of thymidine into dna strands. dna cleavage by bleomycin depends on oxygen and metal ions, at least in vitro. the exact mechanism of dna strand scission is unresolved, but it has been suggested that bleomycin chelates metal ions (primarily iron), producing a pseudoenzyme that reacts with oxygen to produce superoxide and hydroxide free radicals that cleave dna. an alternative hypothesis states that bleomycin may bind at specific sites in the dna strand and induce scission by abstracting the hydrogen atom from the base, resulting in strand cleavage as the base undergoes a criegee-type rearrangement, or forms an alkali-labile lesion. in addition, these complexes also mediate lipid peroxidation and oxidation of other cellular molecules. therefore, bleomycin is used in combination with doxorubicin in hodgkins lymphoma, as they have additive and complementary effects on the dna, since doxorubicin acts by intercalating between dna strands, and also acts on topoisomerase ii enzyme thus relaxing the topoisomerase complexes. biosynthesis bleomycin is a nonribosomal peptide that is a hybrid peptide-polyketide natural product. the peptide/polyketide/peptide backbone of the bleomycin aglycon is assembled by the bleomycin megasynthetase, which is made of both nonribosomal peptide synthetase (nrps) and polyketide synthase (pks) modules. nonribosomal peptides and polyketides are synthesized from amino acids and short carboxylic acids by nrpss and pkss, respectively. these nrpss and pkss use similar strategies for the assembly of these two distinct classes of natural products. both nrps and type i pkss are organized into modules. the structural variations of the resulting peptide and polyketide products are determined by the number and order of modules on each nrps and pks protein. the biosynthesis of the bleomycin aglycon can be visualized in three stages: nrps-mediated formation of p-3a from ser, asn, his, and ala pks-mediated elongation of p-3a by malonyl coa and adomet to yield p-4 nrps-mediated elongation of p-4 by thr to p-5 that is further elongated by β-ala, cys, and cys to get p-6m. on the basis of the bleomycin structure and the deduced functions of individual nrps and pks domains and modules, a linear model for the bleomycin megasynthetase-templated assembly of the bleomycin peptide/polyketide/peptide aglycon was proposed from nine amino acids and one acetate. biosynthesis of bleomycin is completed by glycosylation of the aglycones. bleomycin naturally occurring-analogues have two to three sugar molecules, and dna cleavage activities of these analogues have been assessed, primarily by the plasmid relaxation and break light assays. history