methanofollis methanofollis in taxonomy, methanofollis is a genus of the methanomicrobiaceae. methanofollis scientific classification domain: archaea kingdom: euryarchaeota phylum: euryarchaeota class: methanomicrobia order: methanomicrobiales family: "methanofollaceae" genus: methanofolliszellner et al. 1999 type species methanofollis tationis(zabel, könig & winter 1986) zellner et al. 1999 species m. aquaemaris m. ethanolicus m. fontis m. formosanus m. liminatans "m. propanolicus" m. tationis description and significance methanofollis ("a methane-producing bag") is a non-motile, gram-negative, obligately anaerobic, mesophilic archaeon that produces methane. it grows between the temperatures 20–45 °c (optimum 34–40 °c), and at the ph of around 7. genome structure the genome of the archaeon has not yet sequenced. the g + c content of the dna is determined to be 60.0%. cell structure and metabolism the cells of methanofollis are highly irregular cocci, with diameter of 1.25–2.0 µm. the major polar lipids are phospholipids, glycolipids, and phosphoglycolipids. it utilizes h2/co2, formate, 2-propanol/co2, and 2-butanol/co2 for growth and methanogenesis. no growth has been observed on acetate, trimethylamine, methanol, ethanol, 2-propanol, isobutanol, or 2-butanol as catabolic substrates. ecology most species of the archaeon are isolated from anaerobic high-rate wastewater bioreactors or solfataric fields. for example, m. tationis was isolated from a solfataric field on mount tatio in the atacama desert in northern chile. phylogeny the currently accepted taxonomy is based on the list of prokaryotic names with standing in nomenclature (lpsn) and national center for biotechnology information (ncbi). 16s rrna-based ltp_01_2022 53 marker proteins based gtdb 07-rs207 m. liminatans (zellner et al. 1990) zellner et al. 1999 m. tationis (zabel, könig & winter 1986) zellner et al. 1999 m. fontis chen et al. 2020 m. ethanolicus imachi et al. 2009 m. aquaemaris lai & chen 2001 m. formosanus wu, chen & lai 2005 m. ethanolicus m. liminatans m. tationis