preganglionic nerve fibers preganglionic nerve fibers in the autonomic nervous system, fibers from the cns to the ganglion are known as preganglionic fibers. all preganglionic fibers, whether they are in the sympathetic division or in the parasympathetic division, are cholinergic (that is, these fibers use acetylcholine as their neurotransmitter) and they are myelinated. preganglionic nerve fibersautonomic nervous system innervation, showing the sympathetic and parasympathetic (craniosacral) systems, in red and blue, respectivelydetailsidentifierslatinneurofibrae preganglionicaeta98a14.2.00.009fma76568anatomical terms of neuroanatomy sympathetic preganglionic fibers tend to be shorter than parasympathetic preganglionic fibers because sympathetic ganglia are often closer to the spinal cord than are the parasympathetic ganglia. another major difference between the two ans (autonomic nervous systems) is divergence. whereas in the parasympathetic division there is a divergence factor of roughly 1:4, in the sympathetic division there can be a divergence of up to 1:20. this is due to the number of synapses formed by the preganglionic fibers with ganglionic neurons.