dianthovirus dianthovirus dianthovirus is a genus of viruses, in the family tombusviridae. dianthoviruses are plant viruses. there are three species in this genus. the virus probably has a worldwide distribution, and can be transmitted via nematodes, by mechanical inoculation, by grafting of plants and by contact between infected hosts with previously uninfected host. dianthovirus virus classification (unranked): virus realm: riboviria kingdom: orthornavirae phylum: kitrinoviricota class: tolucaviricetes order: tolivirales family: tombusviridae subfamily: regressovirinae genus: dianthovirus taxonomy the genus contains the following species: carnation ringspot virus red clover necrotic mosaic virus sweet clover necrotic mosaic virus structure viruses in dianthovirus are non-enveloped, with icosahedral and spherical geometries, a “hexagonal” appearance, and t=3 symmetry. the diameter is around 28-34 nm. genomes are linear and segmented, bipartite, around 11.3-1.4kb in length. the buoyant density in cscl of virions is between 1.363 and 1.366 g cm-3. they have a sedimentation coefficient of 126-132-135 s20w. the ph of their isoelectric point is 4.5. the virions become inactive from about 80-90 °c and are inactive above those temperatures. they are viable in vitro for about 50–70 days. treatment with ether either decreases or does not alter their infectivity. no lipids have so far been reported. genusstructuresymmetrycapsidgenomic arrangementgenomic segmentation dianthovirusicosahedralt=3non-envelopedlinearmonopartite life cycle viral replication is cytoplasmic. entry into the host cell is achieved by penetration into the host cell. replication follows the positive stranded rna virus replication model. positive stranded rna virus transcription, using the premature termination model of subgenomic rna transcription is the method of transcription. translation takes place by -1 ribosomal frameshifting. the virus exits the host cell by tubule-guided viral movement. plants serve as the natural host. transmission routes are mechanical, seed borne, and contact. genushost detailstissue tropismentry detailsrelease detailsreplication siteassembly sitetransmission dianthovirusplantsnoneviral movement; mechanical inoculationviral movementcytoplasmcytoplasmmechanical: contact; seed genome these viruses have segmented, bipartite genomes that are linear, positive-sense, single-stranded rna (1). these genomes are about 5300 nucleotides in length (1). they have a methylated cap at the 5'-end whose sequence type is m7gpppa (1). the genome also codes for non-structural proteins as well as structural proteins (1). three non-structural proteins have been found (1).