arthrobacter alkaliphilus arthrobacter alkaliphilus arthrobacter alkaliphilus is a gram-positive and non-spore-forming bacterium species from the genus of arthrobacter which has been isolated from filtrations from a volcanic rock in niigata in japan. arthrobacter alkaliphilus scientific classification domain: bacteria phylum: actinomycetota class: actinomycetia order: micrococcales family: micrococcaceae genus: arthrobacter species: a. alkaliphilus binomial name arthrobacter alkaliphilusding et al. 2009 type strain cctcc ab 206013, iam 15383, jcm 21827, lc6 cell morphology and features arthrobacter alkaliphilus is a non-motile, gram-positive bacteria that demonstrates a rod-coccus growth cycle. this was first observed upon culture of bacteria in genus arthrobacter where a young culture demonstrated an irregular rod shape which was replaced in older cultures by a coccoid form. when those older cultures were placed in a new media they again produced irregular rod shapes. it is this characteristic that makes arthrobacter bacteria unique and hard to identify. in a culture of an arthrobacter alkaliphilus colony scientists observed a round, convex, and glossy appearance with entire margins and a light yellow color. after 2–7 days the culture shows mostly coccoid cells that are 0.6-1.0 micrometers in diameter. in most arthrobacter bacteria the fatty acids found are primarily iso- and anteiso-branched which refers to a branch on the antepenultimate carbon atom of a saturated fatty acid chain. initial testing of bacteria found in volcanic rock showed that the major fatty acid was anteiso-c15 : 0 and the major amino acid present in the cell wall peptidoglycan was l-lysine a common amino acid used in the structure of many living organisms. biochemical characteristics were assessed by linxian ding, taketo hirose, and akira yokota showing results for alkaline and a number of enzymes including; acid phosphatase, catalase, esterase lipase c8, leucine arylamidase, valine arylamidase, typsin, naphthol-as-bi-phosphohydrolase, alpha- and beta-glucuronidase, alpha-glucosidase, alpha-mannosidase, pyrazinamidase, pyrrolidonyl arylamidase, and urease. phylogeny and genome evolution this bacteria belongs to phylum actinomycetota, class actinomycetia, order micrococcaceae, family micrococcaceae, and genus arthrobacter. shown below is the genome evolution for arthrobacter alkaliphilus. also shown is the 1457 bp sequence length and corresponding gc content which is 60 mol%. phylogenetic tree for the genus arthrobacter 1 tggctcagga tgaacgctgg cggcgtgctt aacacatgca agtcgaacga tgaagcctag 61 cttgctgggt ggattaggtg gcgaacgggt gagtaacacg tgagtaacct gcccttgact 121 ctgggataag cctgggaaac tgggtctaat accggatatg actgctccgc gcatgcggtg 181 gtggtggaaa gcttttgcgg ttttggatgg actcgcggcc tatcagcttg ttggtggggt 241 aatggcctac caaggcgacg acgggtagcc ggcctgagag ggtgaccggc cacactggga 301 ctgagacacg gcccagactc ctacgggagg cagcagtggg gaatattgca caatgggcgc 361 aagcctgatg cagcgacgcc gcgtgaggga tgacggcctt cgggttgtaa acctctttca 421 gtagggaaga agctttcggg tgacggtacc tgcagaagaa gcgccggcta actacgtgcc 481 agcagccgcg gtaatacgta gggcgcaagc gttatccgga attattgggc gtaaagagct 541 cgtaggcggt ttgtcgcgtc tgctgtgaaa gaccggggct caactccggt tctgcagtgg 601 gtacgggcag actagagtga tgtaggggag actggaattc ctggtgtagc ggtgaaatgc 661 gcagatatca ggaggaacac cgatggcgaa ggcaggtctc tgggcattaa ctgacgctga 721 ggagcgaaag catggggagc gaacaggatt agataccctg gtagtccatg ccgtaaacgt 781 tgggcactag gtgtggggga cattccacgt tttccgcgcc gtagctaacg cattaagtgc 841 cccgcctggg gagtacggcc gcaaggctaa aactcaaagg aattgacggg ggcccgcaca 901 agcggcggag catgcggatt aattcgatgc aacgcgaaga accttaccaa ggcttgacat 961 ggactagtaa gacgcagaaa tgtgttcccc tctttgaggc tggtttacag gtggtgcatg 1021 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctcg 1081 ttctatgttg ccagcggttc ggccggggac tcataggaga ctgccggggt caactcggag 1141 gaaggtgggg acgacgtcaa atcatcatgc cccttatgtc ttgggcttca cgcatgctac 1201 aatggccggt acaaagggtt gcgatactgt gaggtggagc taatcccaaa aagccggtct 1261 cagttcggat tggggtctgc aactcgaccc catgaagtcg gagtcgctag taatcgcaga 1321 tcagcaacgc tgcggtgaat acgttcccgg gccttgtaca caccgcccgt caagtcacga 1381 aagttggtaa cacccgaagc cggtggccta acccttgtgg agggagccgt cgaaggtggg 1441 accggcgatt gggacta metabolic details the bacdive is a worldwide database that uses cultured data demonstrating arthrobacter’s metabolism and physiology. arthrobacter is a gram-positive bacterium and it requires oxygen to fully utilize its carbon diet. it can consume glucose, lactose, maltose, mannitol, and ribose. arthrobacter alkaliphilus is a subtype of athrobacter that can degrade lindane and use it as a carbon source. it utilizes acid phosphatase, alkaline phosphatase, alpha-galactosidase, catalase, pyrazinamidase, and urease. it is commonly found in volcanic rock at an optimal ph of 8.5, 3-7% nacl, and a temperature of 30 °c. it cannot form spores for reproduction. relevance to broader system the genus arthrobacter is a member of the family micrococcaceae and compared to other genera of the family, it contains the highest number of species. this contributes to the fact that species within arthrobacter are metabolically versatile and can be found in diverse environments. understanding this genus is essential to understanding the impact each has on its environment. the primary impact each has is its ability to degrade different environmental pollutants. one such pollutant, lindane, has shown to accumulate in the adipose tissue of different organisms and is very toxic to aquatic organisms and somewhat toxic to mammals and birds. arthrobacter alkaliphilus’ ability to degrade lindane can then have a major impact on its ecosystem.