mycobacterium psychrotolerans mycobacterium psychrotolerans mycobacterium psychrotolerans is a rapidly growing mycobacterium first isolated from pond water near a uranium mine in spain. it was able to grow at 4°c and is therefore considered to be psychrotolerant. etymology: psychros, cold; tolerans, tolerating. mycobacterium psychrotolerans scientific classification domain: bacteria phylum: actinomycetota class: actinomycetia order: mycobacteriales family: mycobacteriaceae genus: mycobacterium species: m. psychrotolerans binomial name mycobacterium psychrotoleranstrujillo et al. 2004, dsm 44697 description microscopy gram-positive, acid-fast, non-spore-forming, non-motile short rods. colony characteristics smooth, entire, bright orange, scotochromogenic colonies appear after 2 days in gyea, bennett's and nutrient agars. physiology growth on lowenstein–jensen agar is moderate. no growth occurs on macconkey agar. grows at 4–37c and tolerates 7% nacl. the type strain is resistant to ampicillin, cefuroxime, cloxacillin, erythromycin, penicillin and polymyxin. sensitive to ciprofloxacin, gentamicin, neomycin and oxytetracycline. differential characteristics growth at 4°c. pathogenesis first isolated from an environmental source, not known to be pathogenic. type strain the type strain was isolated from a pond in salamanca, spain. strain wa101 = dsm 44697 = jcm 13323 = lmg 21953