kininogen 1 kininogen 1 kininogen-1 (kng1), also known as alpha-2-thiol proteinase inhibitor, williams-fitzgerald-flaujeac factor or the hmwk-kallikrein factor is a protein that in humans is encoded by the kng1 gene. kininogen-1 is the precursor protein to high-molecular-weight kininogen (hmwk), low-molecular-weight kininogen (lmwk), and bradykinin. kng1available structurespdbhuman uniprot search: pdbe rcsb list of pdb id codes1ny2, 2wok, 4asq, 4asr, 4ecb, 4ecc, 5i25identifiersaliaseskng1, bdk, bk, kng, kininogen 1, hmwk, hae6, hkexternal idsomim: 612358 mgi: 3027157 homologene: 88343 genecards: kng1 gene location (human)chr.chromosome 3 (human)band3q27.3start186,717,348 bpend186,744,410 bpgene location (mouse)chr.chromosome 16 (mouse)band16|16 b1start22,804,604 bpend22,848,232 bprna expression patternbgeehumanmouse (ortholog)top expressed inrenal medullaliverright lobe of liverkidney tubulekidneymetanephric glomeruluspancreatic ductal cellrectumprefrontal cortextibialis anterior muscletop expressed inkidneyrenal cortexproximal tubuleliverbone marrowmorulaplacentaesophagusquadriceps femoris musclezone of skinmore reference expression databiogpsmore reference expression datagene ontologymolecular function peptidase inhibitor activity heparin binding zinc ion binding protein binding signaling receptor binding cysteine-type endopeptidase inhibitor activity cellular component blood microparticle plasma membrane extracellular exosome platelet alpha granule lumen extracellular space endoplasmic reticulum lumen extracellular matrix extracellular region collagen-containing extracellular matrix biological process hemostasis blood coagulation, intrinsic pathway negative regulation of proteolysis negative regulation of cell adhesion negative regulation of peptidase activity negative regulation of blood coagulation positive regulation of cytosolic calcium ion concentration platelet degranulation vasodilation positive regulation of apoptotic process inflammatory response blood coagulation negative regulation of endopeptidase activity post-translational protein modification g protein-coupled receptor signaling pathway sources:amigo / quickgoorthologsspecieshumanmouseentrez3827385643ensemblensg00000113889ensmusg00000060459uniprotp01042n/arefseq (mrna)nm_001166451nm_000893nm_001102416nm_001102409nm_001102410nm_201375refseq (protein)np_000884np_001095886np_001159923n/alocation (ucsc)chr 3: 186.72 – 186.74 mbchr 16: 22.8 – 22.85 mbpubmed searchwikidataview/edit humanview/edit mouse expression the kng1 gene uses alternative splicing to generate two different proteins: high-molecular-weight kininogen (hmwk) and low-molecular-weight kininogen (lmwk). hmwk in turn is cleaved by the enzyme kallikrein to produce bradykinin. kng1 gene → low-molecular-weight kininogen (lmwk) protein (contains 427 amino acids) or high-molecular-weight kininogen (hmwk) protein (644 amino acids) hmwk protein → bradykinin peptide (9 amino acids) function hmwk is essential for blood coagulation and assembly of the kallikrein-kinin system. also, bradykinin, a peptide causing numerous physiological effects, is released from hmwk. in contrast to hmwk, lmwk is not involved in blood coagulation. kininogen-1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.