euglobulin lysis time euglobulin lysis time the euglobulin lysis time (elt) is a test that measures overall fibrinolysis. the test is performed by mixing citrated platelet-poor plasma with acid in a glass test tube. this acidification causes the precipitation of certain clotting factors in a complex called the euglobulin fraction. the euglobulin fraction contains the important fibrinolytic factors fibrinogen, pai-1, tissue plasminogen activator (tpa), plasminogen, and to a lesser extent α2-antiplasmin. the euglobulin fraction also contains factor viii. euglobulin lysis timetest ofoverall fibrinolysis after precipitation, the euglobulin fraction is resuspended in a borate solution. clotting is then activated by the addition of calcium chloride at 37 °c. historically, subsequent amount of fibrinolysis was determined by eye, by observing the clot within the test tube at ten-minute intervals until complete lysis had occurred. newer automated methods have also been developed. these methods use the same principle as the older technique, but use a spectrophotometer to track clot lysis as a function of optical density.