hyperpolarized gas mri hyperpolarized gas mri hyperpolarized gas mri, also known as hyperpolarized helium-3 mri or hphe-3 mri, is a medical imaging technique that uses hyperpolarized gases to improve the sensitivity and spatial resolution of magnetic resonance imaging (mri). this technique has many potential applications in medicine, including the imaging of the lungs and other areas of the body with low tissue density. the current standard for diagnosing and monitoring treatment of pulmonary diseases is spirometric pulmonary function testing (pfts). however, these tests only assess the lung on a global basis and are generally not sensitive enough to detect functional changes in the small airways and gas exchange regions. this lack of sensitivity has led these regions to be known as the "silent zone." additionally, pft metrics largely rely on the effort of the subject, leading to significant measurement uncertainty and variability. as a result, current therapy is largely based on patients' symptoms and survival. given the high burden on the healthcare system and the increasing prevalence of pulmonary disease, there is a need for improved diagnostic tools and quantitative metrics to better diagnose and quantify pulmonary disease progression and accurately measure response to therapy. the basic principle of hyperpolarized gas mri is similar to that of conventional mri, which uses powerful magnetic fields and radio waves to create detailed images of the body's internal structures. in conventional mri, the magnetic moments of hydrogen atoms (protons) in the body's water and fat molecules are aligned with the magnetic field and then subjected to a radiofrequency pulse. this causes the protons to absorb energy and become excited, and when the radiofrequency pulse is turned off, the protons relax and release their energy in the form of a detectable signal. this signal is used to construct an image of the body's tissues. overcoming challenges of traditional mri traditional mr imaging of the lungs is difficult because conventional scanners are designed to excite hydrogen protons, which are present in water molecules. however, the lungs have only a very low density of hydrogen protons compared to other structures, and their long relaxation time means that the signal available for imaging is minimal. in addition, the inhomogeneous magnetic environment of the lungs introduces susceptibility artifacts that further complicate mr acquisitions. these challenges are not faced by external gaseous contrast media like 3he or 129xe, which image the airways and airspaces within the lungs rather than the surrounding tissues. this greatly reduces the problems of unfavorable longitudinal and transverse relaxation times faced by hydrogen mris in the lung. however, mr imaging of a gas is challenging because its density is typically about 4 orders of magnitude lower than that of protons. to overcome this limitation, a process called hyperpolarization is used to increase the magnetization of these gases by about 5 orders of magnitude. this makes mr-based imaging of inhaled gases feasible within a single breath hold. to improve the ability to detect early lung disease, it is necessary to use imaging techniques that provide regional information. hyperpolarized gas magnetic resonance imaging (hp gas mri) is a non-invasive, radiation-free method that can image the structure and function of the lungs. while 3he was originally used extensively in hp gas mri, its recent scarcity and increase in price has led to a shift towards the cheaper and more abundant 129xe. the advantage of using 129xe is that it is soluble in pulmonary tissues, providing two additional signal sources in addition to the xenon in the airspaces. these three 129xe resonances can provide quantitative regional information about the fundamental function of the lungs: gas exchange. history and safety in 1994, the first studies on hyperpolarized (hp) gas magnetic resonance imaging (mri) were carried out using the noble gas isotope 129-xenon (129xe). in 1997, mugler and colleagues used 129xe to conduct the first studies in humans. however, these studies were limited by relatively low 129xe polarizations (1-2%), which resulted in low signal intensities. this issue led to a shift in research interest to helium (3he), which has a larger gyromagnetic ratio than 129xe and offers a simpler and more mature polarization technology (30%) and corresponding larger signal intensities. 3he also does not have any physiological side effects, making it a better starting point for clinical imaging. in 1996, 3he mr imaging entered clinical research and expanded to multi-center clinical studies. the results of the ventilation studies showed a significant correlation to conventional pulmonary function tests in patients with chronic obstructive pulmonary disease, asthma, and cystic fibrosis. the main problem with 3he hp mr imaging is the limited supply of 3he, which comes from the decay of tritium, a byproduct of nuclear weapons production. this has driven up costs significantly to around $800–2000 per liter depending on academic versus commercial use. due to these higher costs and lower availability, 3he hp mr imaging is not economically sustainable. recent advances in 129xe polarization technology have led to the reintroduction of 129xe mr imaging in humans. xenon has a long history of safe use as a contrast agent in computed tomography lung imaging studies, which was confirmed in safety studies on inhaling hyperpolarized 129xe. with the development of more efficient polarizers, resulting in improved 129xe polarization, it is expected that better image quality can be achieved with a lower volume of xenon. a second safety study showed that inhalation of only 0.5-liter volumes caused subjects to experience few or no symptoms. physics of hyperpolarization the basic principle of hyperpolarized gas mri is similar to that of conventional mri, which uses powerful magnetic fields and radio waves to create detailed images of the body's internal structures. in conventional mri, the magnetic moments of hydrogen atoms (protons) in the body's water and fat molecules are aligned with the magnetic field and then subjected to a radiofrequency pulse. this causes the protons to absorb energy and become excited, and when the radiofrequency pulse is turned off, the protons relax and release their energy in the form of a detectable signal. this signal is used to construct an image of the body's tissues. in hyperpolarized gas mri, the gases used are noble gases, such as 3he or 129xe, which have large nuclear magnetic moments but low natural abundance and therefore produce very weak signals. to increase the nuclear spin polarization of either 3he or 129xe, two processes are involved: 1) optical pumping and 2) spin exchange. hyperpolarized gas mri is a technique that uses the alignment of nuclear spins in certain gases, such as 3he or 129xe, to create detailed images of the body's internal structures. in order for the nuclear spins to be used for imaging, they must be aligned in the same direction, or polarized. under normal conditions, the nuclear spins within the gas volume are randomly aligned, leading to a zero signal. once the nuclear spins have been polarized, they can be placed in a large magnetic field, such as that of a 1.5t or 3.0t scanner. this will cause slightly more spins to align with the field than against it. however, this difference is not sufficient for imaging dilute gases like 3he or 129xe. therefore, hyperpolarization techniques are used to add angular momentum to the system and align all of the nuclear spins in the same direction, resulting in a strong signal that can be used to create detailed images of the body's tissues. optical pumping hyperpolarization is the process of aligning the nuclear spins in a gas, such as 3he or 129xe, in the same direction to create a strong signal for imaging. to accomplish this, angular momentum is added to the system through the use of circularly polarized laser light. since nuclei cannot directly absorb laser light, an intermediary is used to absorb the light and transfer its angular momentum to the nuclei. this intermediary is typically an alkali metal atom, such as rubidium, whose outer-shell valence electron is aligned by the laser light. only atoms with electron spins that are down can absorb the light, so illuminating the alkali vapor with circularly polarized resonant light will convert the entire sample to the spin up direction. once a valence electron spin has been flipped up, it remains aligned until collisions cause it to depolarize. however, it can simply absorb another photon and return to the aligned state. this process, known as optical pumping, allows for the efficient alignment of nuclear spins in the gas. spin exchange the alignment of the valence electron is then transferred to the noble-gas nuclei through collisions with polarized electron spins of the rubidium. this process is called spin exchange. the rubidium electrons are then aligned again by absorbing additional laser light and continue to build polarization in the noble-gas nuclei. current techniques using optical pumping and spin exchange can achieve polarizations of around 40-80% for 3he and 10-40% for 129xe. recently, very high peak polarization levels for 129xe have been demonstrated in diluted mixtures. mechanism of hyperpolarization the process of optical pumping uses rubidium (rb) contained in a glass optical cell. this cell is placed in an oven with two helmholtz coils that generate a small, but homogenous 20 g magnetic field. the rb is heated to around 150 °c to produce a vapor pressure of about 1ppm of the total gas density in the cell. circularly polarized laser light is then directed at the cell, which is tuned to the d1 transition of rubidium. this light is absorbed by the rb vapor, polarizing the valence electron spins on the rb atoms. spin exchange is a process that begins when a mixture of 1% 129xe, 89% 4he and 10% n2 is directed to flow through an optical cell that contains optically pumped rubidium (rb) atoms. the buffer gases, helium and nitrogen, serve to broaden the rb absorption cross section, allowing a large fraction of laser light to be absorbed and used to polarize the valence electron spins of the rb atoms. through a combination of binary collisions and the formation of transient van der waals complexes, the electron spin polarization is transferred to the 129xe nuclei. the gas flow rate is regulated to ensure that the 129xe emerges from the cell with a high level of polarization. to separate the 129xe from the helium and nitrogen, it is cryogenically accumulated in a cold finger immersed in liquid nitrogen. since xenon has a higher freezing point than the other gases, it is frozen out and separated from them. once a sufficient amount of xenon has been accumulated, it is thawed and dispensed into a perfluoropolymer bag. the xenon polarization is then measured using a low-field nmr-based system and delivered to the patient for use in mri imaging. commercially available systems can produce liters of xenon polarized to 10-15% within an hour. advances in polarization physics are expected to improve both the production rate and polarization of 129xe in the future. in order to obtain images of the subject's lung tissue, the polarized xenon gas is inhaled through a tube connected to a mouthpiece. the subject is instructed to take a deep breath and exhale fully twice before inhaling the gas. the typical scan uses a mixture of 200-1000 ml of 129xe and a buffer gas such as helium or nitrogen. this mixture is inhaled by the subject and used to create detailed images of the lung tissue. applications ventilation imaging hp 3he gas mr imaging of the lungs has been confirmed to be effective in multiple clinical studies since 1997. this technique is mainly used to create images of gas distribution in the lungs, allowing for the identification of ventilation defects. these defects can be caused by blocked airways or destruction of lung tissue. the mr signal intensities in the ventilation images can be grouped into four clusters for analysis. low or absent signal within the lungs corresponds well with ventilation defects and allows for the detection and quantification of functional ventilation impairment in conditions such as asthma, copd, and cystic fibrosis. data acquisition for this technique is completed in a single breath-hold, providing static ventilation information. dynamic ventilation properties, such as gas flow, are more difficult to measure but progress has been made in this area. traditionally, hp 3he provided better image quality due to its larger polarization compared to 129xe. however, recent improvements in polarization technology and mr acquisition have allowed 129xe