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How to identify a queen ant 
Look at the size of the ant. 
For many types of ants, queens are much larger than regular worker ants. If you stumble upon an ant that appears unusually large, there's a good chance this is the queen.

Available options:
[-] Queen ants are nocturnal, and may attack any worker ants in their webs. Queen ants have very small (0.8 in) egg sacs at the bottom of each ant's abdomen.
[-] You'll need to take a closer look at the ant to get to the perfect size for the queen ant. The queen ant has smaller arms and legs than regular worker ants, and even smaller feet to walk towards and across the grounds.
[-] Just to be certain, look for a queen ant with a large head. Take in as much detail as possible about the ant's head.
[-] The ant will be noticeably bigger than the surrounding ants, or other ants you have seen. Think about the type of ants you have as well.
Answer:
The ant will be noticeably bigger than the surrounding ants, or other ants you have seen. Think about the type of ants you have as well.