Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

Input: Consider Input: Everywhere man is altering the balance of nature.He is facilitating the spread of plants and animals into new regions, sometimes deliberately, sometimes unconsciously.He is covering huge areas with new kinds of plants, or with houses, factories, slag-heaps and other products of his civilization.He exterminates some species on a large scale, but favours the multiplication of others.In brief, he has done more in five thousand years to alter the biological aspect of the planet than has nature in five million. Many of these changes which he has brought about have had unforeseen consequences.Who would have thought that the throwing away of a piece of Canadian waterweed would have caused half the waterways of Britain to be blocked for a decade, or that the provision of pot cacti for lonely settlers' wives would have led to Eastern Australian being overrun with forests of Prickly Pear? Who would have prophesied that the cutting down of forests on the Adriatic coasts, or in parts of Central Africa, could have reduced the land to a semidesert, with the very soil washed away from the bare rock? Who would have thought that improved communications would have changed history by the spreading of disease-sleeping sickness into East Africa, measles into Oceania, very possibly malaria into ancient Greece? These are spectacular examples; but examples on a smaller scale are everywhere to be found.We make a nature sanctuary for rare birds, prescribing absolute security for all species; and we may find that some common and hardy kind of bird multiplies beyond measure and ousts the rare kinds in which we were particularly interested.We see, owing to some little change brought about by civilization, the starling spread over the English country-side in hordes.We improve the yielding capacities of our cattle; and find that now they exhaust the pastures which sufficed for less exigent stock.

Output: What have spread diseases?


Input: Consider Input: The Bacillus anthracis toxin genes, cya, lef, and pag, can be viewed as a regulon, in which transcription of all three genes is activated in trans by the same regulatory gene, atxA, in response to the same signal, CO2. In atxA+ strains, toxin gene expression is increased 5- to 20-fold in cells grown in 5% CO2 relative to cells grown in air. CO2-enhanced toxin gene transcription is not observed in atx4-null mutants. Here, we used two independent techniques to obtain evidence for additional CO2-induced atxA-regulated genes. First, total protein preparations from atxA4+ and atxA isolates grown in 5% CO2 and in air were examined by two-dimensional electrophoresis. Comparison of the resulting protein patterns indicated that synthesis of non-toxin proteins is influenced by growth in elevated CO2 and the toxin gene regulator, atxA. Second, we generated random transcriptional lacZ fusions in B. anthracis with transposon Tn917-LTV3. Transposon-insertion libraries were screened for mutants expressing CO2-enhanced atxA-dependent beta-galactosidase activity. DNA sequence analysis of transposon insertion sites in 17 mutants carrying CO2- and atxA-regulated fusions revealed 10 mutants carrying independent insertions on the 185-kb toxin plasmid pXO1 which did not map to the toxin genes. The tcr-lacZ fusion mutants (tcr for toxin coregulated) were Tox+, indicating that these genes may not be involved in anthrax toxin gene activation. Our data indicate a clear association of atxA with CO2-enhanced gene expression in B. anthracis and provide evidence that atxA regulates genes other than the structural genes for the anthrax toxin proteins.

Output: Which metabolite activates AtxA?


Input: Consider Input: The Quai Saint-Michel and Notre-Dame is a 1901 oil on canvas painting by the French artist Maximilien Luce.


Output: What material was used for The Quai Saint-Michel and Notre-Dame?


