Given the task definition and input, reply with output. In this task, you are given a sentence. You are expected to write the name of the organism substances in the body mentioned in the input. Although there might be several correct answers, you need to write one of them.

 At 48 h post - transfection , cell lysates were prepared using 100 mul per well of lysis buffer ( 1 % Triton X - 100 , 1 mm dithiothreitol , 92 . 8 mm K2HPO4 , pH 7 . 8 , 9 . 2 mm KH2PO4 , pH 7 . 8 ) and centrifuged for 5 min at 4 degreesC .

cell lysates