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He made his appeal while celebrating Mass in three native languages after a new Vatican decree approved their use.
The Pope also used the open-air service in San Cristobal de las Casas to warn about threats to the environment.
Throughout his five-day trip he has condemned the evils of forced emigration and drugs.
He has urged Mexico's leaders to provide "true justice" to suffering citizens.
But on Monday he drew attention to the suffering of indigenous people in his address in Chiapas state.
"On many occasions, in a systematic and organised way, your people have been misunderstood and excluded from society," the 79-year-old pontiff said after citing Popol Vuh, an ancient Mayan text.
"Some have considered your values, culture and traditions to be inferior. Others, intoxicated by power, money and market trends, have stolen your lands or contaminated them. How sad this is," he said.
"How worthwhile it would be for each of us to examine our conscience and  learn to say, 'Forgive me!'"
Correspondents say that while Chiapas is the country's least Roman Catholic state, tens of thousands of people attended an open-air Mass in a sports field in San Cristobal de las Casas.
Women wore colourfully embroidered dresses to deliver biblical readings and hymns in the Chol, Tzotzil and Tzeltal languages.
The pontiff highlighted the deep appreciation of indigenous people for nature and said their community had much to teach the rest of the world.
He also highlighted the need to care for the environment.
"We can no longer remain silent before one of the greatest environmental crises in world history," he said,
While Mexico is the world's second most populous Catholic nation after Brazil, only 58% are loyal to the Vatican in Chiapas in comparison to 82% in the rest of the country.
Mexicans hope papal visit brings change
On Tuesday, the Pope heads to the capital of the western state of Michoacan, a region scarred by drug violence.
The Pope concludes his five-day trip in Ciudad Juarez on the US border, a city which has also been blighted by drug-related murders. A Mass there will highlight the plight of migrants.
Pope Francis has asked Mexican indigenous people in Chiapas state for forgiveness over they way they have been excluded from society.

Sam James' converted try cancelled out Ma'a Nonu's opener to give the Sharks a 7-5 first-half advantage.
A Leigh Halfpenny penalty and Bryan Habana's score restored the lead for the three-time champions.
And although Rob Webber's try narrowed the gap, Jonathan Pelissie and Josua Tuisova went over in the last two minutes while Sale were down to 14 men.
Maximum points for the French side, coached by former Bath boss Mike Ford and Leicester director of rugby Richard Cockerill, takes the pressure off for their final pool game against reigning champions Saracens, who have already secured a quarter-final spot.
Winless Sale, meanwhile, have now lost their last 10 matches in all competitions and cannot progress in Europe, but they produced an impressive display against their Top 14 opponents.
The hosts needed only two minutes to get off the mark, as All Black Nonu burst through the defence to touch down, but Halfpenny missed the conversion.
The Sharks pulled themselves ahead when James chipped forward, capitalised on an awkward bounce and raced over the line, with Will Addison adding the extras.
Toulon then lost Halfpenny to the sin-bin for a deliberate knock-on, but he made amends when he kicked his side ahead in the second half.
Sale's hard work began to unravel when full-back Mike Haley's clearance was charged down by Habana and he crossed to score, with Halfpenny converting.
The visitors heaped the pressure back on Toulon after hooker Webber forced his way over the line, but Addison missed the extras, and they soon lost Kieran Longbottom to the sin-bin for a dangerous tackle.
Two quick Toulon breaks sent Pelissie and Tuisova over to make sure of the home win.
Sale head coach Steve Diamond:
"The scoreline didn't reflect the game. The first 60-65 minutes it was a very good game, but their bench made the difference in the end.
"Matt Giteau came on and made a big difference for them, but there was a lot of pride in our performance.  We had 21 English-qualified players in our matchday squad and we are proud of what we are doing at the club.
"A lot of young lads got some exposure to Champions Cup rugby, but what we need now is stability in the Premiership."
Toulon centre Mathieu Bastareaud:
"We're going to go at Saracens with a lot of enthusiasm. But firstly we've got to put the Sale game behind us and then we can start thinking about what is going to be a very big game at Allianz Park.
"We would have loved not to have had the suspense at the finish. We got the victory in the end, but it was a very tough one against a very good team that had nothing to lose."
Toulon: Leigh Halfpenny; James O'Connor, Mathieu Bastareaud, Ma'a Nonu, Bryan Habana; Pierre Bernard, Sebastien Tillous-Borde; Laurent Delboulbès, Guilhem Guirado, Marcel Van Der Merwe; Jocelino Suta, Samu Manoa; Liam Gill, Juan Fernandez Lobbe, Duane Vermeulen (capt)
Replacements: Jean-Charles Orioli, Florian Fresia, Levan Chilachava, Juan Smith, Matt Giteau, Josua Tuisova, Jonathan Pelissie, Mamuka Gorgodze
Sale Sharks: Mike Haley; Paolo Odogwu, Will Addison (capt), Mark Jennings, Josh Charnley; Sam James, Mike Phillips; Ross Harrison, Rob Webber, Halani Aulika; Bryn Evans, Jonathan Mills; Magnus Lund, David Seymour, Laurence Pearce.
Replacements: Neil Briggs, James Flynn, Kieran Longbottom, George Nott, Ben Curry, Tom Curry, James Mitchell, Dan Mugford.
For the latest rugby union news follow @bbcrugbyunion on Twitter.
Toulon's bonus-point win over Sale kept alive their hopes of a place in the last eight of the Champions Cup.

The small town holds great value to IS because of a prophecy of an apocalyptic battle, and features heavily in its propaganda.
The operation comes as US and Russian envoys meet in Switzerland to discuss possible routes to a new ceasefire.
But diplomats have played down hopes of any breakthrough at the talks.
Since a brief truce collapsed last month, Syrian government forces backed by Russian air strikes have intensified their bombardment of rebel-held areas in Aleppo.
Aid agencies say a 72-hour ceasefire is urgently needed to allow supplies in and civilians out of devastated areas in the east of the city.
Turkish President Tayyip Erdogan confirmed fighters were moving on Dabiq, which lies about 10km (6 miles) from the Turkish border.
Rebel fighters backed by Turkish airstrikes have been edging closer to the town for days, seizing villages around it and all but isolating it.
A bombardment was taking place as part of the offensive on Saturday, a monitoring group and a rebel commander said.
Dabiq is important to IS because it is named in Islamic apocalyptic prophecies as the site of an end-of-times showdown between Muslims and their enemies. The militant group named its magazine after the town.
The advance on Dabiq is part of a wider offensive launched by an alliance of Syrian rebel groups, supported by Turkish forces, in late August.
They are trying to drive IS militants and Syrian Kurdish fighters from an area along Turkey's border with Syria. Since it began, they have captured the key towns of Jarablus and al-Rai.
Meanwhile, US Secretary of State John Kerry and Russian Foreign Minister Sergei Lavrov are meeting delegates from Turkey, Saudi Arabia, Iran and Qatar in Lausanne to discuss ways to broker a new ceasefire.
But prospects for success looked bleak. On Friday, Mr Lavrov said he had no "special expectations" for the talks, while a US State Department official told Reuters he did not anticipate a major announcement.
"This is going to be, as it has been now for several years, a very difficult process," the agency quoted the US official as saying.
There can be no peace in Syria without the agreement of the men meeting in Switzerland today.
Bilateral talks between the US and Russia have brought no real solution. A ceasefire brokered last month collapsed after just a few days, since then Syrian government bombing of Aleppo, backed by Russia, has intensified.
On the ground Iranian troops are reportedly supporting Syrian forces. Saudi Arabia and the US have been supplying opposition groups with weapons and money.
Moscow insists rebel forces it regards as terrorists must be separated from more moderate opposition groups before the bombing of Aleppo can stop. Washington says the bombing is a war crime.
And so, even as the talks get under way, the message from the diplomats who could make peace was: don't expect too much.
Syria's President Bashar al-Assad has vowed to "keep cleaning" Aleppo of rebels and told a Russian newspaper that winning in the city would be a "springboard" to winning in the rest of the country.
Ahead of the talks, organisations including Save the Children, Oxfam, the Norwegian Refugee Council and the International Rescue Committee issued a plea "to establish a ceasefire of at least 72 hours in east Aleppo" to allow evacuations and delivery of aid.
There are now no safe areas left in rebel-held parts of the city, according to REACH, an organisation that contacts people there regularly in order to gather humanitarian reports.
About 275,000 people live in the besieged areas, and aid organisations have not been able to get to them since the siege resumed on 4 September.
More than 370 people, including nearly 70 children, have been killed in the bombardment of eastern Aleppo, according to the UK-based Syrian Observatory for Human Rights.
The monitor said dozens of civilians including children have also died in rebel bombardment of western Aleppo, which is controlled by the Syrian government.
A war that started with an uprising against President al-Assad has now split Syria into many parts. It has been going on for over five years and it has claimed 300,000 lives.

Turkish-backed rebels in Syria are advancing on Dabiq, a symbolically important stronghold of so-called Islamic State.

