Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[EX Q]: Many different cellular pathways have evolved to protect the genome from the deleterious effects of DNA damage that result from exposure to chemical and physical agents. Among these is a process called transcription-coupled repair (TCR) that catalyzes the removal of DNA lesions from the transcribed strand of expressed genes, often resulting in a preferential bias of damage clearance from this strand relative to its non-transcribed counterpart. Lesions subject to this type of repair include cyclobutane pyrimidine dimers that are normally repaired by nucleotide excision repair (NER) and thymine glycols (TGs) that are removed primarily by base excision repair (BER). While the mechanism underlying TCR is not completely clear, it is known that its facilitation requires proteins used by other repair pathways like NER. It is also believed that the signal for TCR is the stalled RNA polymerase that results when DNA damage prevents its translocation during transcription elongation. While there is a clear role for some NER proteins in TCR, the involvement of BER proteins is less clear. To explore this further, we studied the removal of 7-methylguanine (7MeG) and 3-methyladenine (3MeA) from the dihydrofolate reductase (dhfr) gene of murine cell lines that vary in their repair phenotypes. 7MeG and 3MeA constitute the two principal N-methylpurines formed in DNA following exposure to methylating agents. In mammalian cells, alkyladenine DNA alkyladenine glycosylase (Aag) is the major enzyme required for the repair of these lesions via BER, and their removal from the total genome is quite rapid. There is no observable TCR of these lesions in specific genes in DNA repair proficient cells; however, it is possible that the rapid repair of these adducts by BER masks any TCR. The repair of 3MeA and 7MeG was examined in cells lacking Aag, NER, or both Aag and NER to determine if rapid overall repair masks TCR. The results show that both 3MeA and 7MeG are removed without strand bias from the dhfr gene of BER deficient (Aag deficient) and NER deficient murine cell lines. Furthermore, repair of 3MeA in this region is highly dependent on Aag, but repair of 7MeG is equally efficient in the repair proficient, BER deficient, and NER deficient cell lines. Strikingly, in the absence of both BER and NER, neither 7MeG nor 3MeA is repaired. These results demonstrate that NER, but not TCR, contributes to the repair of 7MeG, and to a lesser extent 3MeA.
[EX A]: Which gene strand is targeted by transcription-coupled repair (TCR)?

[EX Q]: The water droplets of fog form from water vapor in the air. Fog disappears when the water droplets change back to water vapor. These changes are examples of changes of state. A change of state occurs whenever matter changes from one state to another. Common states of matter on Earth are solid, liquid, and gas. Matter may change back and forth between any two of these states. Changes of state are physical changes in matter. They are reversible changes that do not change matters chemical makeup or chemical properties. For example, when fog changes to water vapor, it is still water and can change back to liquid water again. Several processes are involved in common changes of state. They include melting, freezing, sublimation, deposition, condensation, and evaporation. The Figure 1.1 shows how matter changes in each of these processes. Q: Which two processes result in matter changing to the solid state? A: The processes are deposition, in which matter changes from a gas to a solid, and freezing, in which matter changes from a liquid to a solid. Suppose that you leave some squares of chocolate candy in the hot sun. A couple of hours later, you notice that the chocolate has turned into a puddle like the one pictured in the Figure 1.2. Q: What happened to the chocolate? A: The chocolate melted. It changed from a solid to a liquid. In order for solid chocolate to melt and change to a liquid, the particles of chocolate must gain energy. The chocolate pictured in the Figure 1.2 gained energy from sunlight. Energy is the ability to cause changes in matter, and it is always involved in changes of state. When matter changes from one state to another, it either absorbs energyas when chocolate meltsor loses energy. For example, if you were to place the melted chocolate in a refrigerator, it would lose energy to the cold air inside the refrigerator. As a result, the liquid chocolate would change to a solid Q: Why is energy always involved in changes of state? A: The energy of particles of matter determines the matters state. Particles of a gas have more energy than particles of a liquid, and particles of a liquid have more energy than particles of a solid. Therefore, in order for matter to change from a solid to a liquid or from a liquid to a gas, particles of matter must absorb energy. In order for matter to change from a gas to a liquid or from a liquid to a solid, particles of matter must lose energy.
[EX A]: which process does not involve matter in the liquid state?

[EX Q]: The circumsporozoite protein (CS protein) on the malaria parasites in mosquitoes plays an important role in sporogony in mosquitoes. The RTS,S/AS01 malaria vaccine candidate, which has shown significant efficacy against clinical malaria in a large Phase 3 trial, targets the Plasmodium falciparum CS protein, but the ability of serum from vaccinated individuals to inhibit sporogony in mosquitoes has not been evaluated. Previously a double-blind, randomized trial of RTS,S/AS01 vaccine, as compared with rabies vaccine, in five- to 17-month old children in Tanzania was conducted. In this study, polyclonal human antibodies were purified from the pools of sera taken one month after the third vaccination. IgGs were purified from four pools of sera from 25 RTS,S/AS01 vaccinated children each, and two pools of sera from 25 children vaccinated with rabies vaccine each. The ability of antibodies to inhibit P. falciparum oocyst formation and/or sporogony in the mosquito host was evaluated by a standard membrane-feeding assay. The test antibodies were fed on day 0 (at the same time as the gametocyte feed), or on days 3 or 6 (serial-feed experiments). The oocyst and sporozoite counts were performed on days 8 and 16, respectively. In addition, two human anti-CS monoclonal antibodies (mAb) and a control mAb were also evaluated. Polyclonal anti-CS IgG preparations from RTS,S-vaccinated children tested at concentrations of 149-210 ELISA units (EU)/ml did not show significant inhibition in oocyst and sporozoite formation when the antibodies were fed with gametocytes at the same time, or later (serial-feed experiments). Similarly, anti-CS mAbs tested at 6,421 or 7,122 EU/ml did not show reduction in oocyst and sporozoite formation. This study does not support the concept that anti-CS antibodies induced by the RTS,S/AS01 vaccines in humans noticeably reduce malaria transmission by blocking P. falciparum sporozoite development or salivary gland invasion in mosquitoes when taken up during feeding.
[EX A]:
RTS S AS01 vaccine was developed to prevent which disease?

