Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[Q]: In eukaryotic cells, there are two sub-pathways of nucleotide excision repair (NER), the global genome (gg) NER and the transcription-coupled repair (TCR). TCR can preferentially remove the bulky DNA lesions located at the transcribed strand of a transcriptional active gene more rapidly than those at the untranscribed strand or overall genomic DNA. This strand-specific repair in a suitable restriction fragment is usually determined by alkaline gel electrophoresis followed by Southern blotting transfer and hybridization with an indirect end-labeled single-stranded probe. Here we describe a new method of TCR assay based on strand-specific-PCR (SS-PCR). Using this method, we have investigated the role of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase-related protein kinases (PIKK) family, in the TCR pathway of UV-induced DNA damage. Although depletion of DNA-PKcs sensitized HeLa cells to UV radiation, it did not affect the ggNER efficiency of UV-induced cyclobutane pyrimidine dimers (CPD) damage. We postulated that DNA-PKcs may involve in the TCR process. To test this hypothesis, we have firstly developed a novel method of TCR assay based on the strand-specific PCR technology with a set of smart primers, which allows the strand-specific amplification of a restricted gene fragment of UV radiation-damaged genomic DNA in mammalian cells. Using this new method, we confirmed that siRNA-mediated downregulation of Cockayne syndrome B resulted in a deficiency of TCR of the UV-damaged dihydrofolate reductase (DHFR) gene. In addition, DMSO-induced silencing of the c-myc gene led to a decreased TCR efficiency of UV radiation-damaged c-myc gene in HL60 cells. On the basis of the above methodology verification, we found that the depletion of DNA-PKcs mediated by siRNA significantly decreased the TCR capacity of repairing the UV-induced CPDs damage in DHFR gene in HeLa cells, indicating that DNA-PKcs may also be involved in the TCR pathway of DNA damage repair. By means of immunoprecipitation and MALDI-TOF-Mass spectrometric analysis, we have revealed the interaction of DNA-PKcs and cyclin T2, which is a subunit of the human transcription elongation factor (P-TEFb). While the P-TEFb complex can phosphorylate the serine 2 of the carboxyl-terminal domain (CTD) of RNA polymerase II and promote transcription elongation. A new method of TCR assay was developed based the strand-specific-PCR (SS-PCR). Our data suggest that DNA-PKcs plays a role in the TCR pathway of UV-damaged DNA. One possible mechanistic hypothesis is that DNA-PKcs may function through associating with CyclinT2/CDK9 (P-TEFb) to modulate the activity of RNA Pol II, which has already been identified as a key molecule recognizing and initializing TCR.
[A]: Which gene strand is targeted by transcription-coupled repair (TCR)?


[Q]: After winning in a close game at home, the Steelers traveled to Cincinnati for Game 2 against the Bengals. The Bengals scored first in the first quarter when Randy Bullock kicked a 35-yard field goal to make it 3-0. They would then make it 10-0 when Andy Dalton found A.J. Green on an 8-yard pass. In the second quarter, Dalton and Green connected again on a 15-yard pass to make it 17-0. The Steelers managed to get on the board before halftime when Chris Boswell put a 30-yard field goal through to make the score 17-3. In the third quarter, the Steelers drew closer when Ben Roethlisberger found Le'Veon Bell on a 35-yard touchdown to make it 17-10. The Bengals moved back ahead by double digits when Randy Bullock kicked a 31-yard field goal to make it 20-10. In the fourth quarter, it was all Steelers when Boswell put up a 37-yard field goal to make it 20-13. Roethlisberger then found Antonio Brown on a 6-yard pass to tie the game up at 20-20. Finally, Boswell was able to seal the victory with a 38-yard field goal to make the final score 23-20. With their sixth straight win over the Bengals, the Steelers improved to 10-2. The victory marks the third time that the Steelers have recorded two different 7+ win streaks within consecutive and separate regular seasons (1975-76, 1994-95). The team also won their 8th straight game against a divisional opponent. With the win, the Steelers began 4-0 in their division for the first time since 2008. The 17-point comeback was the Steelers' largest since they came back from down 17 on December 20, 2015 against the Denver Broncos, and their largest on the road since their 21-point comeback on October 5, 1997 against the Baltimore Ravens.
[A]: Which player kicked the second-longest field goal?


[Q]: It was believed that Myoma Nyein committed suicide in 1955, aged 46, by walking in front of a lorry.
[A]:
In one word, how did Myoma Nyein die?


