Feasible and reproducible synthesis of full-length Aâ peptides has been one of the major challenges in Alzheimer's disease research. By using dimethyl sulfoxide as an anti-aggregation solvent and as an agent to promote double-coupling of two phenylalanine that frequently experience residual deletion, we developed a reliable manual Fmoc solid phase peptide synthesis procedure to produce biologically active Aâ in large quantities at relatively high purity. The amyloidogenic activity of the synthesized Aâ was confirmed via thioflavin T assay, transmission electron microscopic analysis and electrophoresis.
Amino Acids Amyloid