OBJECTIVE: To design oligonucleotide probes for microarray detection of dengue virus.METHODS: By analyzing the cDNAs of dengue viruses of 4 different serotypes with BLAST program, a group of specific sequences for the candidate probes was acquired. Oligo6.0 software was applied to analyze the candidates to select the probes with high specificity, identical length and similar melting temperature (Tm).RESULT: Altogether 48 oligonucleotide probes were designed, and deposited on oligonucleotide chips as the microarray for dengue virus detection.CONCLUSION: BLAST program and Oligo6.0 software are simple and effective means for designing the oligonucleotide probes.
Base Sequence Computational Biology