DNA from herpes simplex virus type II (HSV-II)-infected cells isolated by using SDS, phenol, and pronase as well as by a new method of Pignatti et al. from triton X-100-NaCl-supernatant was shown to be capable to induce production of an early nonvirion (new) antigen in sensitive systems of Vero, RS-537 cells and others. It was also established that as a result of transfection of DNA recovered from 13 speciment of cancer tumors of the cervix and ovaries (9 tumors contained the new antigen and 4 were free from it), the new antigen associated with HSV-II was synthesized in 8 cases in the sensitive cells as early as 6 hours postinoculation whereas no viral antigens could be detected by this time. No viral antigens could be detected within 24 hours either, with the exception of 3 cases where CPE also developed by 24 hours indicating the appearance of the virus. DNA recovered from normal tissues of the cervix was incapable of inducing the new antigen.
Antigens Cell Line