Plasma membranes from insulin or insulin mimicker (hydrogen peroxide, anti-insulin receptor antibody, and concanavalin A) treated adipocytes showed an increase in glucose transport compared to control cells due to an increase in Vmax and not due to alteration in Km. Arrhenius plots showed no difference in the energy of activation between control and insulin or insulin mimicker stimulated glucose transport states. Glucose transport by plasma membranes from control or treated adipocytes was equally (percentage) inhibited by N-ethylmaleimide, reduced glutathione, or cytochalasin B. The data indicate that the increased transport resulted from addition of new transport sites similar to the sites existing in the basal state.
Adipose Tissue Animals