Three new spirostane-type glycosides (1-3) were isolated from the whole plant of Allium flavum. Their structures were elucidated mainly by 2D NMR spectroscopic analysis and mass spectrometry as (20S,25R)-2á-hydroxyspirost-5-en-3â-yl O-â-D-xylopyranosyl-(1?3)-[â-D-galactopyranosyl-(1?2)]-â-D-galactopyranosyl-(1?4)-â-D-galactopyranoside (1), (20S,25R)-2á-hydroxyspirost-5-en-3â-yl O-â-D-xylopyranosyl-(1?3)-[â-D-glucopyranosyl-(1?2)]-â-D-galactopyranosyl-(1?4)-â-D-galactopyranoside (2), and (20S,25R)-spirost-5-en-3â-yl O-á-L-rhamnopyranosyl-(1?4)-[â-D-glucopyranosyl-(1?2)]-â-D-glucopyranoside (3). The three saponins were evaluated for cytotoxicity against a human cancer cell line (colorectal SW480).
Allium Antineoplastic Agents, Phytogenic