The objective of the study was to evaluate the concentration of fluoride in human enamel before and after bleaching using 10-15% carbamide peroxide and after application of the fluoride varnish. Microsamples of enamel were collected using acid biopsy and the concentration of fluoride in the superficial and underlying layer was assayed. No significant differences were observed in the concentration of fluoride in both layers of enamel before and after bleaching as well as after varnish application. Bleaching with 10-15% carbamide peroxide has no influence on the concentration of fluoride in enamel.
Biopsy Carbamide Peroxide