Autophagy is a cellular process which occurs in eukaryotic cells. To study the mechanism regulating polyploid fish growth and development is of significance in genetic, because of its growth advantages and economic values. This study focused on triploid female rainbow trout (RBT) which discusses the effects of autophagy on gonadal development of polyploid fish. Autophagy-related genes of RBT lc3b, atg12, atg4b, gabarap1, and bcl2 were cloned, and autophagy gene expressions in gonads were analyzed at different developmental period. Gonadal ultrastructures were observed under transmission electron microscopy. To detect autophagy protein expression and localization, antibodies of RBT-LC3B and RBT-ATG12 were produced. Results showed clear evidence that autophagy-related genes were highly expressed during 200-300 days post fertilization (dpf), in which autophagosome structures were identified. In this stage, the conversion of LC3B-I to LC3B-II was greater than those in other stages. Immunolabeling-manifested autophagy occurred intensively in the cytoplasm of follicular cells. The morphology of follicular cells was gradually changed, leading to gonadal fibrosis and regression. This autophagic research is a new study area on gonadal development of polyploid fish.
Animals Antibodies