Lotus seed epicarp (LSE) and lotus seed pot (LSP) were characterized, and a total of 5 and 7 proanthocyanidins (PAs) were identified in purified LSE and LSP extract, respectively. Purified LSE and LSP PAs significantly suppressed the body weight and weight gain of white adipose tissue (WAT) and decreased the WAT cell size in high-fat diet-induced obese mice regardless of the daily food intake. LSE or LSP administration significantly lowered the serum leptin level and improved the serum and liver lipid profiles (including total cholesterol (TC), triglycerides (TG), high-density lipoprotein-cholesterol (HDL-C), and low-density lipoprotein-cholesterol (LDL-C) levels), increased activities of antioxidant enzymes (superoxide dismutase (SOD) and glutathione transferase (GST)) and reduced glutathione (GSH) concentration, and suppressed lipid peroxidation in hepatic tissue. LSP PAs were generally more effective than LSE PAs. Both extracts ameliorated obesity, insulin resistance, and oxidative damage in obese mice, suggesting they are good candidates for value-added functional food and nutraceutical ingredients.
Animals Anti-Obesity Agents