A replication study with some extensions was made to confirm enhancement of ornithine decarboxylase (ODC) activity in murine L929 fibroblasts after radiofrequency (RF) field exposure reported in earlier studies. L929 cells purchased from two cell banks were exposed for 2, 8, or 24 h to continuous wave or DAMPS (burst modulated at 50 Hz, with 33% duty cycle) signals at specific absorption rate (SAR) levels of 2.5 or 6.0 W/kg. Exposures were carried out in Crawford and waveguide chambers, at frequencies 835 and 872 MHz, respectively. The results did not confirm findings of previous studies reporting increased ODC activity in RF-exposed cells. When Crawford cell exposure system was used, ODC activity was either not affected (in the case of 8 or 24 h exposures) or decreased after 2 h exposure at the highest SAR level (6 W/kg). The decrease was most pronounced when cooling with air flow was not used, and is most likely related to increased temperature. The minor methodological differences (use of antibiotics, increased sensitivity of ODC assay) are not likely to explain the inconsistency of the findings of the present and previous studies. Different results were obtained in experiments with the waveguide system that involves more efficient temperature control. In this exposure system, ODC activity was increased after 8 h exposure at 6 W/kg. Further studies are warranted to explore whether this finding reflects a true non-thermal effect. The present study did not provide evidence for modulation-specific effects reported in earlier studies.
Animals Cell Line