The chromatographic behavior of a new HPLC-stationary phase with supramolecular selectors on the basis of calixarenes is described for the separation of nine tricyclic neuroleptics. The effects of different chromatographic conditions (buffer system, pH-value, type and content of organic modifier, injection volume) on the separation of the analytes were studied. Additionally, the effect of structural differences of the neuroleptic analytes was studied. The chemical structure and pKa of the neuroleptics highly influenced their separation on the calix[8]arene phase. The separation of all analytes on the investigated calixarene-bonded stationary phase was possible with a mobile phase of acetonitrile with 30 mM ammonium acetate buffer (pH 3.5) 30:70(v/v) using 1 ml/min flow rate.
Antidepressive Agents, Tricyclic Buffers