Studies of the pig muscle lactate dehydrogenase (LDH) activity were permormed after incubating the enzyme solution with extracts of loach (Misgurnus fossilis) eggs and embryos, which were subjected to the influence of insulin hydrocortisone as well as to insulin combination with actinomycin D, cycloheximide or puromycin. The insulin alone is established to decrease the inactivating ability of the investigated extracts on the lactate dehydrogenase activity, when antibiotics removed to a considerable extent the influence of hormone. In the eggs and embryos there are proteins activating and stabilizing the LDH molecule. The level of LDH activation under the influence of the eggs and embryos extracts subjected to the insulin action was decreased. The addition of hydrocortisone to the medium for the incubation of eggs and embryos does not affect significantly the LDH-inactivating ability of their extracts.
Animals Cycloheximide