Cultured mouse liver cells containing either eipzootic diarrhea of infant mice viral antigen or Nebraska calf diarrhea vaccine virus antigen were equally effective for detecting serum antibody to epizootic diarrhea of infant mice virus by immunofluorescence. The presence of rotavirus in infant mouse intestinal suspensions used as cell culture inocula was confirmed by polyacrylamide gel electrophoresis of the double-stranded RNA viral genome. When the immunofluorescence assay was tested in a limited comparison with an enzyme-linked immunosorbent assay using simian rotavirus SA 11 viral antigen, the fluorescence test was specific but less sensitive than the enzyme-linked immunosorbent assay.
Animals Animals, Suckling