OBJECTIVE: To investigate bacterial culture practices in veterinary clinics, with an emphasis on laboratory biosafety and on quality of laboratory practices.DESIGN: Survey-based prospective study.SAMPLE POPULATION: 166 veterinarians.PROCEDURES: Veterinarians were recruited through the Veterinary Information Network (an Internet-based network restricted to veterinary personnel). All Network-registered veterinarians were eligible to participate. A standardized questionnaire regarding bacterial culture practices in veterinary clinics was completed electronically by study participants.RESULTS: 720 veterinarians completed the survey; 166 (23%) indicated that bacterial culture was performed in his or her clinic. Clinic practices ranged from preliminary aerobic bacterial culture only with submission of isolates to a diagnostic laboratory for further testing (93/160 [58%]) to bacterial culture, identification, and antimicrobial susceptibility testing (19/160 [12%]). Most commonly, urine samples were cultured (151/162 [93%] clinics). Several problematic practices were identified regarding quality and quality control, including inadequate facilities, equipment, supervision, interpretation of data, and culture methods. Biosafety infractions were also common, including inadequate laboratory location, lack of biosafety protocols, and dangerous disposal practices. Ninety-four percent of respondents stated that continuing education regarding culture practices and laboratory safety would be useful.CONCLUSIONS AND CLINICAL RELEVANCE: Data confirmed that bacterial culture was commonly performed in clinics, but that major deficiencies in laboratory methods were widespread. These could result in negative effects on testing quality and increased risk of laboratory-acquired infections among clinic personnel. Veterinary practices in which bacterial cultures are performed must ensure that adequate equipment, facilities, personnel, and training are provided to enable accurate and safe sample testing.
Animals Bacteriological Techniques