BACKGROUND: Peroxisome proliferator-activated receptor ã (PPARã) is a member of the PPAR nuclear hormone receptor superfamily, which plays a crucial role in carcinogenesis. Wnt/â-Catenin signaling pathway has been well certified to contribute to the progression of gastric malignancies. â-Catenin mediates transcriptional regulation by forming a complex with LEF/TCF transcription factors, resulting in activation of downstream target genes such as TERT, ENAH. In this study, we aimed at detecting the effect of PPARã on TERT, ENAH and explaining the further mechanisms of PPARã on tumor suppression.METHODS: The pEGFP-N1-PPARã recombinant plasmid has already been constructed by researchers in our laboratory. We stably transfected it into three gastric cancer (GC) cell lines (MKN-28, SGC-7901 and BGC-823). CCK-8 and transwell assay were employed to analyze the capability of cell proliferation and metastasis. The mRNA and protein levels were evaluated by real-time PCR and western blot analysis.RESULTS: After transfected with PPARã overexpression plasmid, the ability of cell proliferation and migration declined significantly (p<0.05). The expression of PPARã increased (p<0.05) and â-Catenin was inhibited obviously (p<0.05) in the group of pEGFP-N1-PPARã plasmid transfection. Meanwhile, the mRNA or protein levels of TERT and ENAH were suppressed (p<0.05) in pEGFP-N1-PPARã plasmid transfection group compared with control groups.CONCLUSION: PPARã might inhibit the proliferation and migration of GC cell lines through suppressing the expression of TERT and ENAH. PPARã played an important role as a physiological regulator and might be a target for the treatment of GC.
Cell Line, Tumor Cell Movement