We reinvestigated the reaction of dGuo in the presence of nitrous acid by reversed phase HPLC (RPLC) analysis. When 10 mM deoxyguanosine (dGuo) was treated with 100 mM NaNO2 in 3.0 M acetate buffer (pH 3.7) at 37 degrees C, unknown peak appeared in large amount (yield 19.7% at 2 h, 28.7% at 6 h). The yield of this newly found compound, denoted as 1, arose with increasing H+ concentration and was maximal at 50 degrees C. 1 has small depurination rate constant. If no enzymatic repair system for 1 exists, 1 would have strong mutagenic ability.
Chromatography, High Pressure Liquid Deoxyguanosine