The in vivo phagocytosis test, as described by Dunn et al., allows only a limited number of measurements because the preparations do not last long. That's why it was tested, whether deepfreezing allows the evaluation of the preparations at a later time. The results show, that there were no differences between the counting of phagocytosed cells in fresh and in deep frozen macrophages, so that the experiments can be rationalized importantly by using the freezing technique.
Animals Escherichia coli