The author describes a simple and entirely automatic technique for the estimation of triglycerides in human serum which, in acetic medium, are extracted by a mixture of nonane-isopropanol and saponified. The glycerol is oxidised into formaldehyde which is estimated by fluorimetry after Hantzsch' reaction. Successive stages are discussed together with the precision of the method. The results are in agreement with those obtained by Kessler and Lederer's method.
Autoanalysis Female