The results of the isolation, quantitation and biotyping of 120 strains of vaginal Lactobacillus are presented. The more effective culture media for isolation and different methods of pH measurement were investigated. Five different species of Lactobacillus were isolated in vaginal specimens (Lactobacillus acidophilus 88.8%). The size limits found in the 120 Lactobacillus acidophilus strains were 0.8 to 6,0 mu length and 0.3 to 0.8 mu width. The effort to correlate number of Lactobacillus to pH and weight of vaginal fluid did not give satisfactory results because of difficulties in collecting homogeneous samples. Biochemical investigation of 120 vaginal strains of Lactobacillus did not show any peculiar biotype. The A.A. suggested the "routine" detection of vaginal lactobacillar flora as a usefull marker of ecological status in the choice of the therapy.
Adolescent Adult