OBJECTIVE: Evaluation of serum beta-2-microglobulin (beta2M) in an automated analyzer.DESIGN AND METHODS: The DakoCytomation beta2M kit is an antibody based reagent intended for quantitative determination of beta2M in serum and plasma by rate nephelometry.RESULTS: The limit of blank is 0.16 mg/L. The method is linear up to 17.9 mg/L. The imprecision ranged from 2.1% to 7.9% at the concentrations of 1.77 and 7.19 mg/L, respectively. Method comparison yielded slope=1.009, r=0.998. No interference was observed from hemolytic or icteric specimens. Reference interval of a healthy population was 1.13 mg/L to 3.04 mg/L.CONCLUSION: The DakoCytomation reagent is acceptable to measure serum beta2M.
Adult Aged