The antioxidative activity of stobadine and vinpocetine was studied in vitro by measuring their inhibition effect on the depolymerization of the high-molecular-weight hyaluronan by hydroxyl radicals. The radicals were generated by the Cu(2+)-H2O2 system. Hyaluronan depolymerization was monitored by means of size exclusion chromatography. The antioxidative activity of stobadine and vinpocetine was compared to that of D-mannitol. A 50% inhibition of hyaluronan depolymerization was reached at stobadine and vinpocetine concentrations of 1.7 x 10(-6) and 3.0 x 10(-7) mol l-1, respectively, while a D-mannitol level of 2.6 x 10(-3) mol l-1 was needed to achieve the same inhibitory effect.
Antioxidants Carbolines