The hypothalamus of conscious, freely moving rats was superfused with artificial CSF through push-pull cannulae and the release of endogenous histamine was determined radioenzymatically in the superfusate. Superfusion with potassium chloride enhanced the release rate of histamine. The effect of potassium chloride was abolished by alpha-fluoromethylhistidine. Noradrenaline (alpha 1- and alpha 2-agonist) and clonidine (alpha 2-agonist) decreased the release rate of histamine and inhibited the potassium-induced histamine release. In preliminary experiments, yohimbine (alpha 2-antagonist) seemed to increase the release rate of histamine in the superfusate. beta-Agonists and antagonists (isoprenaline, salbutamol, propranolol) did not influence the release of histamine.
Animals Catecholamines