OBJECTIVE: To investigate the role of NF-êB inhibitor in occurence and development of AML.METHODS: AML and normal bone marrow samples were collected from 8 AML patients and 8 normal persons. The expression of NF-êB signaling pathway genes was detected by NF-êB PCR array. Then, AML mouse model was constructed to test the role of NF-êB inhibitor in AML.RESULTS: The NF-êB signal pathway was activated in AML patients. The up-regulated genes, EDARADD, TNFSF14, could activate the NF-êB signal pathway, IL6 could regulate the inflammatory signal. The down-regulated genes, TNFRSF 10B, TNFRSF1A, could lead to cell apoptosis. the AML mouse model was constructed successfully. Then administration of NF-êB inhibitor reduced the inhibition of leukemia niche to the normal hematopoietic stem cells (HSCs), promoted the HSC to enter into cell cycle.CONCLUSION: The NF-êB signal pathway is activated in AML cells. AML mouse model is constructed successfully. NF-êB inhibitor has a potential to treat AML and promotes the HSC to enrter into cell cycle.
Animals Apoptosis