On addition of cytochrome c to a AOT reverse micellar solution, the percolation process usually observed at high temperatures and surfactant concentrations, occurs at room temperature. This is observed either at relatively high water content at a given cytochrome c concentration or at low content on increasing the cytochrome c concentration. On increasing the water content a phase transition is observed with two optically transparent phases. A similar phase transition is observed on solubilizing various enzymes. The temperature of the transition appears to be strongly dependent on the location of the macromolecule in the reserve micelle.
Chymotrypsin Cytochrome c Group