Hemoglobinuria occurs in rats, but not in mice, after i.p. application of Tropidechis carinatus venom. By gel filtration on Sephadex G-75 and G-50 and chromatography on SP-Sephadex C-25 and DEAE-cellulose, a coagulant factor and a phospholipase A were isolated from the venom which in combination only, produced hemoglobinuria.
Animals Chromatography, Gel