Objective To investigate the influence of different fluid shear stress (FSS) on Wnt/â signaling pathway in human umbilical vein endothelial cells (HUVECs) and its relationship with atherosclerosis. Methods HUVECs were subjected to different FSS (0, 1, 15 dynes/cm2) for various durations (6, 12, 18, 24 hours) using a shear stress device. Subsequently, real-time quantitative PCR was used to observe the mRNA expression levels of dishevelled 2 (Dvl2) and â-catenin in the Wnt signaling pathway, and immunofluorescence technology to observe their protein expression and localization. Results The low FSS promoted the expression and recruitment of Dvl2 as well as the translocation of â-catenin into the nucleus at the early stage of loading low FSS. Conversely, laminar FSS inhabited their expression and nuclear translocation of â-catenin. Conclusion Wnt signaling pathway participates in the stress response of vascular endothelial cells to low shear stress.
Cells, Cultured Dishevelled Proteins