Genetically lean and obese swine were used to investigate the control of preadipocyte growth in culture by porcine serum. Sera were collected from fetuses from obese and lean strains at 70, 90 and 110 d of gestation. Postnatal serum samples were collected from both lines of pigs at 23 to 27 kg. Rat preadipocytes were isolated and grown in culture. Preadipocyte and stromal-vascular cell proliferation was greater in cultures grown in sera obtained postnatally than in cultures grown in sera from fetuses. Sera from lean and obese fetuses were equipotent in promoting cell proliferation. Glycerol-phosphate dehydrogenase (GPDH) activity was higher in cultures fed serum from obese pigs and fetuses than in cultures fed serum from lean pigs and fetuses. Cultures grown in serum from obese fetuses and pigs had soluble protein levels similar to cultures grown with serum from lean pigs and fetuses. These results demonstrate that serum from genetically obese swine, in the pre-obese (fetal) and obese (postnatal) state, caused increased adipogenic activity in adipocytes in culture.
Adipose Tissue Animals