The purpose of this study was to determine the role of cAMP in the growth of FRTL and FRTL5 cells, 2 continuous cultured thyroid lines. TSH, at concentrations similar to those reported to induce growth in primary dog thyroid cultures, played an essential role for growth. Stimulators of adenylate cyclase, cholera toxin and forskolin, and cAMP analogues, dibutyryl cAMP and 8-bromo cAMP, mimicked the effect of TSH in both groups of cultured cells. The present data confirm the role of TSH in controlling growth of both cell lines and suggest that cAMP is an essential intracellular mediator of TSH action.
1-Methyl-3-isobutylxanthine Animals