A simple staining method for collagen in semi-thin sections of nerves is described. This technique consists of applying basic fuchsin (0.05%) solution for 2 min. to +/- 1.25 microns plastic resin-embedded sections after routine toluidine blue staining. This staining method clearly demonstrates the amount and orientation of collagenous connective tissue in the nerve, both in transverse and longitudinal sections.
Animals Collagen