Objective To investigate the changes of apoptosis-associated genes in human lung adenocarcinoma cell line A549 after treated by the recombinant human interleukin-24 (rhIL-24) combined with cisplatin (DDP). Methods The mRNA levels of Bax, Bcl-2, survivin, caspase-3, retinoblastoma (Rb) gene, p53 were analyzed by GenomeLabTM GeXP genetic analysis system after A549 cells were treated with 160 ng/mL rhIL-24 or 3 ìg/mL DDP or 160 ng/mL rhIL-24 combined with 3 ìg/mL DDP. Results The rhIL-24 up-regulated the levels of Bax, Rb, caspase-3 mRNAs and down-regulated Bcl-2, survivin mRNAs, but the change of p53 mRNA was not regular. The combination of rhIL-24 and DDP strengthened the above changes. Conclusion The rhIL-24 protein can induce the apoptosis of A549 cells by up-regulating Bax, caspase-3, Rb mRNAs and down-regulating Bcl-2, survivin mRNAs.
A549 Cells Apoptosis