Clophen A-60 was isolated from trioleate by a modification of the method of Veierov and Aharonson. In comparison with the original method the change included the replacement of the glass set for separation of the organic phase with Hehner's cylinder, and in place of petrol ether n-hexane was used. The reproducibility of the method at two levels of fortification was high--the variability index at ICB level 0.200 mg/kg was 4.5% and at ICB level 1000 mg/kg was 6.8%. The calculated relative percent error was at the lower fortification was 3.6% on average, and 7.8% at the higher fortification. In view of the short time necessary for the test, low cost, very good degree of purification, high precision and accuracy this method may be used for separation of polychlorinated diphenyls from fats.
Fats Methods