In homogenates of rat tissues hydrolysis of O-heptyl-O-orthonitrophenylmethyl phosphonate, O-pentyl-O-orthonitrophenylmethyl phosphonate and O-heptyl-O-isopentyl-O-orthonitrophenylphosphate was studied. Hydrolysis of the preparation was shown to be carried out mainly in liver tissue and slso in kidney and blood serum. The substances studied were cleaved only at the bond P-O-aryl. The structure of orthonitrophenyl phosphoorganic substances exhibited the distinct effect on Km and on the maximal rates of the enzymatic hydrolysis. In all the tisses studied O-heptyl-O-orthonitrophenylmethyl phosphonate was hydrolyzed with maximal rate.
Animals Brain