The biological degradability (Zahn-Wellens test) of ethylenediamine derivatives with carboxymethyl and 2-hydroxyethyl groups was investigated. Mixed bacterial culture (activated sludge) was used as inoculum (non-adapted sludge and sludge adapted at different mean biomass retention time, the so-called sludge age). Biodegradability of ethylene(propylene)di(tri)amine-based complexing agents depends on the character and number of substituents and nitrogen atoms in the molecule. Tetra(penta)substituted derivatives with two or more tertiary nitrogen atoms and carboxymethyl or 2-hydroxyethyl groups in the molecule (EDTA, DTPA, PDTA, HEDTA) are very stable from an environmental point of view. On the contrary, disubstituted derivatives with two secondary nitrogen atoms in the molecule (e.g., EDDA) are potentially degradable.
Bacteria Biodegradation, Environmental