The recent development of High Throughput Sequencing technology has boosted the study of small regulatory RNA populations. A critical step prior to cloning and sequencing is purification of small RNA populations. Here, we report the optimization of an anion-exchange chromatography procedure in order to purify small regulatory RNAs bound on proteins. We developed this procedure to make it less time-consuming since our improved method no longer requires specific equipment and can easily be performed at the bench. We believe that our procedure will increase the robustness and accuracy of small RNA libraries in the future.
Animals Base Sequence