A method is described for determination of the volume of fibroblasts, grown in suspension, in normal medium, in medium supplemented with sucrose (Su) 0,04 and 0.08 M. A 4 days permanence in these last two mediums causes an obvious cytoplasmic vacuolation and an increase in cell volume (Tab.1) that reaches its maximum for cells grown in Su 0.08M (87,2%) increase in comparison with control cells). The cell volume determination was performed utilizing a Coulter ZBI Counter coupled with a P64 pulse height analyzer and X-Y recorder. The volume and number of cells for every counting channel of P64 were manually determined from recorder plot (Fig. 1). These data were then elaborated using a desk computer to obtain the mean cell volume, the coefficients of skewness and kurtosis (algorithm for grouped data).
Cells, Cultured Culture Media