Frozen Sections (Cryo-section)
Cryo-sectioning is a technique for cutting frozen tissue samples which are then collected on glass microscope slides. The tissue is rapidly frozen by floating the sample above liquid nitrogen. Samples are cut at temperatures ranging between -
Frozen Sections (Cryo-section)
Cryo-sectioning is a technique for cutting frozen tissue samples which are then collected on glass microscope slides. The tissue is rapidly frozen by floating the sample above liquid nitrogen. Samples are cut at temperatures ranging between -24o C to -28o C. The thickness we cut at depends on the researchers needs, from 16µm to 30 µm. For large tissue such as mouse brains or adult fish trunk, the sections are collected singly. Smaller tissues such as fish embryo, are embedded in an in-house blend of agar/agarose. The tissue is then sectioned a