($\nabla$). []{data-label="f2"}](Fig4)

Effect of Energy Depletion
--------------------------

Next, we study how energy depletion of the protein solution affects the sequence of adsorption. In experiments the energy is controlled by adding sodium azide, or other depletion-energy chemical agents, to the protein solution [@Shapero2011]. Here, for sake of simplicity, we decrease $T$, reducing the kinetic energy of the solution, but neglecting possible effects of the protein stability (Fig. \[f2\]).

We find ($i$) that, although the surface affinity of Fib is stronger than that for IgG, the latter becomes the dominant protein adsorbed on the surface for long time scales; ($ii$) that, by changing $X_P$, the time scale of the process becomes longer, but the inversion of the protein concentration is always present. Hence, the energy depletion leads to an inversion of the Vroman effect.

By comparing the results at different energies, $k_BT$, and same $X_P$ (Fig. \[f1\]-\[f2\]), we observe only a week energy-dependence of the times at which each $C_i^S$ reaches its maximum. Hence, the time-scales of the process are mainly controlled by the total plasma concentration $X_P$, while the slowing-down due to the reduced diffusion seems to be less relevant.

![Same data at $T=120$ K as in Fig. \[f2\] now separated 