actin filaments[@claessens], mixture of actin and DNA [@Negishi] which are confined in small emulsion droplet, also confirm the segregation state and formation of a cortex close to the surface of the droplet under strong confinement; similar to the segregated chain at the periphery in our simulations, Fig. \[fig:N\_dens\](d).

The number density plots, Fig. \[fig:N\_dens\], show when the persistence length of the segment is greater than or comparable to the length of the segment, extra intra-stiffness has a minor effect as short-normal and short-stiff segments behave similarly. By increasing the degree of confinement, Fig. \[fig:N\_dens\](a-c), the flexible chain is pushed more towards the boundary due to the lowest entropic cost and interestingly, except for the strong confinement case, in all other cases its number density close to the center remains relatively unchanged. For strong confinement we also see that the number densities for shorter segments and flexible chains are similar except for the behavior close to the wall where the flexible chain is strongly suppressed, but there is still a finite probability for shorter segments to be at the periphery.

In order to make sure that the segregation is not artifact of the simulation and the polymer chains are not trapped 