for this during structure determination, but modal methods for studying diffraction leave options open [@Quiney2011].

Since damage has been measured in nanocrystallography experiments, it is worth drawing a distinction between damage in crystals and in single molecules. In a crystal, damage ionizes and displaces ions differently in each unit cell, so that the diffraction contains an average over many different damage scenarios. For a single molecule, there is only one damage scenario per measurement and hence we expect a bigger standard deviation of diffraction of single molecules than of nanocrystals. Additionally, nanocrystals are much larger than single molecules, so that the rates at which electrons are trapped is different and the time it takes for a photoelectron to escape is longer. The water that surrounds a nanocrystal injected via a liquid jet [@DePonte2008] also contributes to the damage in the form of additional photoelectrons and secondary electrons. It is proposed to inject single molecules via aerosol injection [@Bogan2010], so that they are surrounded by vacuum, because the background water scattering from a liquid jet would dominate the diffraction from the molecule. For these reasons, damage experiments on single molecules, independent of those on crystals, are needed to draw conclusions for 