In a plot that follows the conventions of the late nineteenth century tragic mulatto genre, Iola struggles to elude the intentions of her various owners to use her sexually. After she is freed by the Union Army during the war, she seeks to find her scattered family members. Embracing her African heritage, she works to improve the social and economic condition of blacks in the United States.
Iola is supported in her struggle by people who relate to various aspects of her complicated life: a devoted former Leroy family slave, Tom Anderson, rescued Iola from a lecherous master. Her brother Harry Leroy joins her in refusing to "pass" as white, although that would make life easier for them. (Note: Both Leroys have a majority of white ancestry.) She meets a newfound uncle, Robert Johnson, who introduces her to her dark-skinned maternal grandmother Harriet, of mostly African descent.