The PFKFB3 gene encodes for a bifunctional protein that plays a critical role in regulating glycolysis through its dual enzymatic activity: it synthesizes and degrades fructose-2,6-bisphosphate (F2,6BP). Below is a detailed overview, including the additional information you requested regarding associated genes, aging-related aspects, drug interactions, and pathways.

### 1. Associated Genes
PFKFB3 is associated with several regulatory and metabolic genes, particularly those involved in glycolysis and cell cycle regulation. Some of these genes include:
- **PFKFB1**: Another gene encoding a bifunctional enzyme that also regulates fructose-2,6-bisphosphate levels.
- **PFKFB2**: Related to PFKFB1 and PFKFB3, involved in glycometabolism.
- **PKM**: Pyruvate kinase, implicated in the final steps of glycolysis.
- **CDK1**: Cyclin-dependent kinase 1, which is directly regulated by PFKFB3 and critical for cell cycle progression.
- **PTEN**: Phosphatase that plays a role in cellular signaling and metabolism.

### 2. Aging and Age-Related Diseases
PFKFB3 has been implicated in several aspects of aging and age-related diseases:
- **Cancer**: Altered expression of PFKFB3 has been observed in various cancers, indicating its role in tumor metabolism. Its regulation can lead to increased cell proliferation and resistance to apoptosis, traits commonly seen in cancer cells.
- **Metabolic Disorders**: As a regulator of glucose metabolism, PFKFB3 may be involved in conditions like diabetes and metabolic syndrome, which are associated with aging.
- **Tissue Repair and Regeneration**: Studies suggest that PFKFB3 may influence stem cell function and tissue regeneration, potentially affecting longevity and age-related functional decline.

### 3. Drug Interactions
Several drugs and drug classes may interact with pathways involving PFKFB3:
- **Metformin**: Known to influence cellular energy metabolism, it may indirectly affect PFKFB3 activity through pathways related to AMP-activated protein kinase (AMPK).
- **Anticancer drugs**: Compounds that target glycolytic pathways in tumor cells may interact with the pathways regulated by PFKFB3, providing a potential therapeutic angle in cancer treatment.
- **PKM2 inhibitors**: If targeted, they could aim to modify the glycolytic flux influenced by PFKFB3, particularly in cancer metabolism.

### 4. Pathways and Biological Processes
PFKFB3 is involved in various biological pathways and processes:
- **Glycolysis**: As a key regulator of fructose-2,6-bisphosphate, PFKFB3 directly impacts glycolytic flux and energy generation.
- **Cell Cycle Regulation**: Modulates cyclin-dependent kinase activity, critical for cell cycle progression.
- **Apoptosis**: Its activity influences survival pathways, particularly in cancer cells, where it helps to prevent programmed cell death.
- **Metabolic Signaling**: Involved in signaling pathways that respond to nutrient availability and stress conditions.

In summary, PFKFB3 serves as a crucial link between glucose metabolism and cell proliferation, with broader implications in aging, cancer biology, and metabolic health. Its interactions with other genes, drugs, and pathways highlight its importance as a potential target for therapeutic interventions in various diseases.