### RNF114 Gene Overview

**Gene Symbol:** RNF114  
**Location:** Cytosol and plasma membrane  
**Function:** E3 ubiquitin-protein ligase, enabling ubiquitin protein ligase activity.

### Additional Gene Associations

In addition to the key interactions mentioned in the summaries (such as with TNFAIP3 and TRAF6), RNF114 may also be associated with the following genes through its role in ubiquitination and cellular processes:

1. **MAVS (Mitochondrial Antiviral Signaling Protein)**: Known target for RNF114-promoted degradation.
2. **CDKN1A (p21)**: Ubiquitinated by RNF114, regulating cell cycle control.
3. **CDKN1B (p27)** and **CDKN1C (p57)**: Potentially involved in their ubiquitination and subsequent degradation.
4. **NF-kappa-B** signaling pathway components, where RNF114 helps regulate transcriptional responses.

### Aging and Age-Related Diseases

- RNF114's role in regulating apoptosis, immune function, and cell cycle progression is critical in the context of aging and age-related diseases, as dysregulation in these processes can lead to various age-related conditions, such as cancer, neurodegeneration, and immune senescence.
- Its involvement in cellular senescence-related mechanisms through the degradation of cell cycle inhibitors (e.g., CDKN1A) may contribute to age-related decline in tissue regeneration and function.
- Studies suggest a potential link between RNF114 expression levels and age-related cellular dysfunction, although specific findings and mechanisms remain an active area of research.

### Drug Interactions

As of the latest data available, there are no specific drugs or drug classes that have been conclusively shown to interact with RNF114. However, given its role in pathways involving inflammation and immune responses, drugs targeting NF-kappa-B signaling, interferon production, or cell cycle regulators may indirectly influence RNF114 activity. Future research may identify more direct pharmacological interactions.

### Pathways and Biological Processes

RNF114 is involved in various pathways and biological processes, including:

1. **Ubiquitin-Proteasome Pathway**: Essential for protein turnover and quality control.
2. **Cell Cycle Regulation**: By ubiquitinating CDK inhibitors (such as CDKN1A), it facilitates transitions from G1 to S phase and regulates cell proliferation.
3. **Apoptosis**: RNF114's role in regulating apoptosis contributes to cellular responses under stress and inflammatory conditions.
4. **Immunity**:
   - **Negative Regulation of NF-kappa-B**: Acts by stabilizing TNFAIP3, leading to modulation of inflammatory responses.
   - **T-cell Activation**: Functions as a negative regulator.
   - **Innate and Adaptive Immunity**: Involved in mechanisms to regulate immune responses, including targeting MAVS during viral challenges.

5. **Osteoclastogenesis**: Participation highlights a role in bone remodeling and diseases like osteoporosis.

In summary, RNF114 is a multifunctional E3 ubiquitin ligase with significant implications in male fertility, cellular regulation, and potential associations with aging and age-related conditions. Its intricate involvement in various biological pathways emphasizes its importance in health and disease. Further research will clarify its roles and potential therapeutic implications.