### WASHC2C Gene Overview

**Gene Symbol:** WASHC2C  
**Location:** Cytosol, early endosome, nucleolus  
**Function:** WASHC2C is a crucial component of the WASH (Wiskott-Aldrich Syndrome protein and SCAR homolog) complex, operating mainly as a nucleation-promoting factor at the surface of endosomes. It facilitates actin polymerization by recruiting the Arp2/3 complex, which is essential for vesicle scission during endosomal sorting.

### Gene Associations

1. **Genes Associated with WASHC2C:**
   - **VPS35:** A subunit of the retromer complex that interacts with WASHC2C to aid in endosomal transport.
   - **DNAJC13, ENTR1, ANKRD50, COMMD1, CCDC93, and VPS35L:** Genes involved in the recruitment and functioning of various endosomal complexes that interact with WASHC2C.
   - **SNX27:** A member of the sorting nexins linked to retromer-dependent cargo trafficking.
  
  These associations highlight the role of WASHC2C in cargo trafficking and sorting processes within the endosomal system.

### Aging and Age-Related Diseases

- **Aging:** The WASH complex, including WASHC2C, has been implicated in the regulation of cellular trafficking, which is crucial in maintaining cellular homeostasis. Proper endosomal function and vesicle trafficking are necessary for processes such as nutrient uptake, membrane recycling, and synaptic function.
- **Age-Related Diseases:** Dysregulation of WASHC2C and the endosomal pathways it influences could be linked to neurodegenerative diseases (e.g., Alzheimer's disease) and conditions like diabetes, where GLUT1 trafficking is affected.

### Drug Interactions

As of now, specific drug interactions with WASHC2C are not directly documented. However, potential drugs affecting the retromer complex or modulating actin polymerization (such as certain chemotherapeutics or small molecules targeting cytoskeletal dynamics) might indirectly influence the functional pathways involving WASHC2C. Further investigation into specific compounds that target the pathways associated with WASHC2C may reveal more pertinent interactions.

### Pathways and Biological Processes

- **Pathways:**
  - **Endosomal Transport:** WASHC2C is involved in the sorting and transport of cargo from endosomes to the plasma membrane.
  - **Actin Polymerization:** By promoting actin nucleation, WASHC2C plays a role in cytoskeletal dynamics, particularly relevant during vesicle scission.
  
- **Biological Processes:**
  - **Regulation of Substrate Adhesion-Dependent Cell Spreading:** Mediating cellular responses to the extracellular environment.
  - **Endosome-to-Plasma Membrane Trafficking:** Essential for recycling integral membrane proteins and maintaining cellular signaling.
  - **Negative regulation of barbed-end actin filament capping:** Influences actin dynamics, necessary for various cellular processes, including motility and division.

In summary, WASHC2C plays a vital role in cellular trafficking dynamics, particularly in endosomal pathways, and is connected to processes affecting aging and neurodegenerative diseases. Further studies are warranted to clarify its potential as a therapeutic target.