끓인 추출물 30마이크로그램은 10% 또는 12% SDS-PAGE 겔로 분리되었으며, 분리된 단백질은 폴리비닐리덴 불소막으로 전달되었다.
Thirty micrograms of boiled extract was separated with 10% or 12% SDS-PAGE gels, and the separated proteins were transferred to polyvinylidene fluoride membranes.