희석액 190 μL에 검액 10 μL를 가하여 vortex로 충분히 혼합한 후 암상태의 실온에서 2시간 동안 반응시켰다.
10 μL of the sample solution and 190 μL of the diluted solution were sufficiently mixed with a vortex and reacted for 2 hours at room temperature in a dark state.