PCR 사이클링 조건은 SureCycler 8800 Therm Cycler에서 35주기 동안, 98°C 30초, 50°C 30초, 72°C 40초였다.
The PCR cycling conditions were 98°C for 30s, 50°C for 30s, and 72°C for 40s, for 35 cycles in a SureCycler 8800 Thermal Cycler.