24시간 동안 37℃, 5% C2에서 배양한 후, 배지를 제거하고, 여러 농도의 각 시료가 포함된 새로운 배지를 넣었다.
After culturing at 37°C and 5% C2 for 24 hours, the medium was removed, and a new medium containing each sample of various concentrations was added.