채취한 혈액은 30분간 상온에서 굳힌 뒤 3,000rpm에서 15분간 원심분리 후 상등액을 취해 혈청을 분리하였다.
The collected blood was solidified at room temperature for 30 minutes, centrifuged at 3,000 rpm for 15 minutes, and the supernatant was taken to separate the serum.