of jeffrey gafoor of the murder of lynette white in the united kingdom on 4 july 2003. dna evidence was matched to gafoor's nephew, who at 14 years old had not been born at the time of the murder in 1988. it was used again in 2004 to find a man who threw a brick from a motorway bridge and hit a lorry driver, killing him. dna found on the brick matched that found at the scene of a car theft earlier in the day, but there were no good matches on the national dna database. a wider search found a partial match to an individual; on being questioned, this man revealed he had a brother, craig harman, who lived very close to the original crime scene. harman voluntarily submitted a dna sample, and confessed when it matched the sample from the brick. as of 2011, familial dna database searching is not conducted on a national level in the united states, where states determine how and when to conduct familial searches. the first familial dna search with a subsequent conviction in the united states was conducted in denver, colorado, in 2008, using software developed under the leadership of denver district attorney mitch morrissey and denver police department crime lab director gregg laberge. california was the first state to implement a policy for familial searching under then-attorney general jerry brown, who later became governor. in his role as consultant to the familial search working group of the california department of justice, former alameda county prosecutor rock harmon is widely considered to have been the catalyst in the adoption of familial search technology in california. the technique was used to catch the los angeles serial killer known as the "grim sleeper" in 2010. it wasn't a witness or informant that tipped off law enforcement to the identity of the "grim sleeper" serial killer, who had eluded police for more than two decades, but dna from the suspect's own son. the suspect's son had been arrested and convicted in a felony weapons charge and swabbed for dna the year before. when his dna was entered into the database of convicted felons, detectives were alerted to a partial match to evidence found at the "grim sleeper" crime scenes. david franklin jr., also known as the grim sleeper, was charged with ten counts of murder and one count of attempted murder. more recently, familial dna led to the arrest of 21-year-old elvis garcia on charges of sexual assault and false imprisonment of a woman in santa cruz in 2008. in march 2011 virginia governor bob mcdonnell announced that virginia would begin using familial dna searches. at a press conference in virginia on 7 march 2011, regarding the east coast rapist, prince william county prosecutor paul ebert and fairfax county police detective john kelly said the case would have been solved years ago if virginia had used familial dna searching. aaron thomas, the suspected east coast rapist, was arrested in connection with the rape of 17 women from virginia to rhode island, but familial dna was not used in the case. critics of familial dna database searches argue that the technique is an invasion of an individual's 4th amendment rights. privacy advocates are petitioning for dna database restrictions, arguing that the only fair way to search for possible dna matches to relatives of offenders or arrestees would be to have a population-wide dna database. some scholars have pointed out that the privacy concerns surrounding familial searching are similar in some respects to other police search techniques, and most have concluded that the practice is constitutional. the ninth circuit court of appeals in united states v. pool (vacated as moot) suggested that this practice is somewhat analogous to a witness looking at a photograph of one person and stating that it looked like the perpetrator, which leads law enforcement to show the witness photos of similar looking individuals, one of whom is identified as the perpetrator. critics also state that racial profiling could occur on account of familial dna testing. in the united states, the conviction rates of racial minorities are much higher than that of the overall population. it is unclear whether this is due to discrimination from police officers and the courts, as opposed to a simple higher rate of offence among minorities. arrest-based databases, which are found in the majority of the united states, lead to an even greater level of racial discrimination. an arrest, as opposed to conviction, relies much more heavily on police discretion. for instance, investigators with denver district attorney's office successfully identified a suspect in a property theft case using a familial dna search. in this example, the suspect's blood left at the scene of the crime strongly resembled that of a current colorado department of corrections prisoner. partial matches partial dna matches are the result of moderate stringency codis searches that produce a potential match that shares at least one allele at every locus. partial matching does not involve the use of familial search software, such as those used in the united kingdom and the united states, or additional y-str analysis and therefore often misses sibling relationships. partial matching has been used to identify suspects in several cases in both countries and has also been used as a tool to exonerate the falsely accused. darryl hunt was wrongly convicted in connection with the rape and the murder of a young woman in 1984 in north carolina. surreptitious dna collecting police forces may collect dna samples without a suspect's knowledge, and use it as evidence. the legality of the practice has been questioned in australia. in the united states, where it has been accepted, courts often rule that there is no expectation of privacy and cite california v. greenwood (1988), in which the supreme court held that the fourth amendment does not prohibit the warrantless search and seizure of garbage left for collection outside the curtilage of a home. critics of this practice underline that this analogy ignores that "most people have no idea that they risk surrendering their genetic identity to the police by, for instance, failing to destroy a used coffee cup. moreover, even if they do realize it, there is no way to avoid abandoning one's dna in public." the united states supreme court ruled in maryland v. king (2013) that dna sampling of prisoners arrested for serious crimes is constitutional. in the united kingdom, the human tissue act 2004 prohibits private individuals from covertly collecting biological samples (hair, fingernails, etc.) for dna analysis but exempts medical and criminal investigations from the prohibition. england and wales evidence from an expert who has compared dna samples must be accompanied by evidence as to the sources of the samples and the procedures for obtaining the dna profiles. the judge must ensure that the jury must understand the significance of dna matches and mismatches in the profiles. the judge must also ensure that the jury does not confuse the match probability (the probability that a person that is chosen at random has a matching dna profile to the sample from the scene) with the probability that a person with matching dna committed the crime. in 1996 r v. doheny juries should weigh up conflicting and corroborative evidence, using their own common sense and not by using mathematical formulae, such as bayes' theorem, so as to avoid "confusion, misunderstanding and misjudgment". presentation and evaluation of evidence of partial or incomplete dna profiles in r v bates, moore-bick lj said: we can see no reason why partial profile dna evidence should not be admissible provided that the jury are made aware of its inherent limitations and are given a sufficient explanation to enable them to evaluate it. there may be cases where the match probability in relation to all the samples tested is so great that the judge would consider its probative value to be minimal and decide to exclude the evidence in the exercise of his discretion, but this gives rise to no new question of principle and can be left for decision on a case by case basis. however, the fact that there exists in the case of all partial profile evidence the possibility that a "missing" allele might exculpate the accused altogether does not provide sufficient grounds for rejecting such evidence. in many there is a possibility (at least in theory) that evidence that would assist the accused and perhaps even exculpate him altogether exists, but that does not provide grounds for excluding relevant evidence that is available and otherwise admissible, though it does make it important to ensure that the jury are given sufficient information to enable them to evaluate that evidence properly. dna testing in the united states cbp chemist reads a dna profile to determine the origin of a commodity. there are state laws on dna profiling in all 50 states of the united states. detailed information on database laws in each state can be found at the national conference of state legislatures website. development of artificial dna in august 2009, scientists in israel raised serious doubts concerning the use of dna by law enforcement as the ultimate method of identification. in a paper published in the journal forensic science international: genetics, the israeli researchers demonstrated that it is possible to manufacture dna in a laboratory, thus falsifying dna evidence. the scientists fabricated saliva and blood samples, which originally contained dna from a person other than the supposed donor of the blood and saliva. the researchers also showed that, using a dna database, it is possible to take information from a profile and manufacture dna to match it, and that this can be done without access to any actual dna from the person whose dna they are duplicating. the synthetic dna oligos required for the procedure are common in molecular laboratories. the new york times quoted the lead author, daniel frumkin, saying, "you can just engineer a crime scene ... any biology undergraduate could perform this". frumkin perfected a test that can differentiate real dna samples from fake ones. his test detects epigenetic modifications, in particular, dna methylation. seventy percent of the dna in any human genome is methylated, meaning it contains methyl group modifications within a cpg dinucleotide context. methylation at the promoter region is associated with gene silencing. the synthetic dna lacks this epigenetic modification, which allows the test to distinguish manufactured dna from genuine dna. it is unknown how many police departments, if any, currently use the test. no police lab has publicly announced that it is using the new test to verify dna results. researchers at the university of tokyo integrated an artificial dna replication scheme with a rebuilt gene expression system and micro-compartmentalization utilizing cell-free materials alone for the first time. multiple cycles of serial dilution were performed on a system contained in microscale water-in-oil droplets. chances of making dna change on purpose overall, this study's artificial genomic dna, which kept copying itself using self-encoded proteins and made its sequence better on its own, is a good starting point for making more complex artificial cells. by adding the genes needed for transcription and translation to artificial genomic dna, it may be possible in the future to make artificial cells that can grow on their own when fed small molecules like amino acids and nucleotides. using living organisms to make useful things, like drugs and food, would be more stable and easier to control in these artificial cells. on july 7, 2008, the american chemical society reported that japanese chemists have created the world's first dna molecule comprised nearly completely of synthetic components. the results might lead to breakthroughs in gene therapy, nano-sized computers of the future, and other technological advancements. a nano-particle based artificial transcription factor for gene regulation: nano script is a nanoparticle-based artificial transcription factor that is supposed to replicate the structure and function of tfs. on gold nanoparticles, functional peptides and tiny molecules referred to as synthetic transcription factors, which imitate the various tf domains, were attached to create nano script. we show that nano script localizes to the nucleus and begins transcription of a reporter plasmid by an amount more than 15-fold. moreover, nano script can successfully transcribe targeted genes onto endogenous dna in a nonviral manner. three different fluorophores—red, green, and blue—were carefully fixed