dca agar dca agar dca agar (deoxycholate citrate agar) is a solid bacteriological growth medium used for isolation of enteric pathogens. a microbiological culture of salmonella sp. bacteria on dc agar culture medium uses it is particularly useful for the isolation of organisms that cause bacillary dysentery, salmonella strains that cause food poisoning and salmonella paratyphi. it is not so selective for salmonella typhi. this growth medium is inhibitory to most gut bacteria, in particular species of the genus proteus, although these species do survive on dca agar. it is therefore essential that suspected pathogens must be subcultured on a less inhibitory medium prior to identification. salmonella spp appear to be yellow or colourless colonies, often with a dark centre. as there are many bacteria that also look like salmonella on dca, it is widely recommended that more selective agars are used for the identification of salmonella, namely xylose lysine deoxycholate (xld) agar. this growth medium is heat-sensitive and should be poured and cooled as soon as possible after addition of the deoxycholate, otherwise it tends to become very soft and difficult to handle. it has a ph of approximately 7.3, and when poured and cooled, appears light to dark pink in colour. contents one liter of deoxycholate citrate agar contains: sodium citrate 20 grams agar 17 grams lactose 10 grams solids from meat infusion 10 grams peptic digest of animal tissue 10 grams sodium deoxycholate 5 grams ferric citrate 1 gram neutral red 20 milligrams