tympanocentesis tympanocentesis tympanocentesis is the drainage of fluid from the middle ear usually caused by otitis media, by using a small-gauge needle to puncture the tympanic membrane, also known as the eardrum. tympanocentesisspecialtyotolaryngology main indication for tympanocentesis is failed treatment with antibiotics it is sometimes referred to as a "tap" and, when conducted twice as part of a clinical trial of medication, a "double tap."