solibacillus solibacillus solibacillus is a genus of gram positive, rod shaped, spore-forming bacteria. solibacillus scientific classification domain: bacteria phylum: bacillota class: bacilli order: caryophanales family: caryophanaceae genus: solibacilluskrishnamurthi 2009 species s. cecembensis s. isronensis s. kalamii s. silvestris the first member of solibacillus was first isolated in 1999, and was originally called bacillus silvestris. however, further studies on b. silvestris found that the organism belonged in a separate genus. solibacillus silvestris was proposed, referring to its original designation as a member of bacillus, and the fact that the species was first isolated from soil. bacillus isronensis was later reclassified as solibacillus isronensis. s. isronensis was isolated from cryotubes that were used to collect air samples from high altitudes, and s. kalamii was found in an air filter from the international space station. in 2020, the family planococcaceae was merged with the family caryophanaceae due to nomenclature rules under the international code of nomenclature of prokaryotes. in addition, the family caryophanaceae underwent several taxonomic changes to account for the large number of phylogenetically unrelated species that were misclassified. using comparative genomic analyses, the study identified several unique molecular markers (specifically conserved signature indels) could serve as a reliable means to distinguish genera such as solibacillus within the family. molecular signatures 12 conserved signature indels (csis) were identified through genomic analyses for this genus in the following proteins: flagellar hook–basal body protein, aminodeoxychorismate lyase, voc family protein, dna topoisomerase iv subunit a, degv family protein, helicase–exonuclease addab subunit addb, multidrug resistance efflux transporter family protein, heme-dependent peroxidase, methionine abc transporter atp-binding protein, trna 4-thiouridine(8) synthase thii, and aaa family atpase. these csis provide a reliable molecular method for distinguishing solibacillus species from other genera within the family caryophanaceae and all other bacteria.