acholeplasma laidlawii acholeplasma laidlawii acholeplasma laidlawii are small bacteria which lack a cell wall. like other acholeplasma and mycoplasma, a. laidlawii has been identified as a common contaminant of growth media for cell culture. acholeplasma laidlawii scientific classification domain: bacteria phylum: mycoplasmatota class: mollicutes order: acholeplasmatales family: acholeplasmataceae genus: acholeplasma species: a. laidlawii binomial name acholeplasma laidlawii(sabin 1941) edward and freundt 1970 history a. laidlawii was first isolated from sewage in london in 1936 and was named after its discoverer, patrick laidlaw. genetics a. laidlawii has a relatively small genome comprising 1.5mbp. additionally its genome has a low gc-content of just 31%. the a. laidlawii genome has been sequenced. in research acholeplasma laidlawii may contaminate bovine serum and also occurs in serum-free cell culture media products. the presence of a. laidlawii in broth powders is a serious problem in routine biopharmaceutical operations where filtration is used as a sterilisation procedure. a. laidlawii may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media.