netropsin netropsin netropsin (also termed congocidine or sinanomycin) is a polyamide with antibiotic and antiviral activity. netropsin was discovered by finlay et al., and first isolated from the actinobacterium streptomyces netropsis. it belongs to the class of pyrrole-amidine antibiotics. netropsin names iupac name n--4--1-methyl-1h-pyrrole-2-carboxamide other names nt, congocidin, sinanomycin identifiers cas number 1438-30-8 y 3d model (jsmol) interactive image chembl chembl553984 n chemspider 4306 n echa infocard 100.162.288 pubchem cid 4461 unii 64b3o0rd7n y comptox dashboard (epa) dtxsid00879834 inchi inchi=1s/c18h26n10o3/c1-27-9-11(6-12(27)16(30)23-4-3-14(19)20)26-17(31)13-5-10(8-28(13)2)25-15(29)7-24-18(21)22/h5-6,8-9h,3-4,7h2,1-2h3,(h3,19,20)(h,23,30)(h,25,29)(h,26,31)(h4,21,22,24) nkey: idbiffksxlyuot-uhfffaoysa-n ninchi=1/c18h26n10o3/c1-27-9-11(6-12(27)16(30)23-4-3-14(19)20)26-17(31)13-5-10(8-28(13)2)25-15(29)7-24-18(21)22/h5-6,8-9h,3-4,7h2,1-2h3,(h3,19,20)(h,23,30)(h,25,29)(h,26,31)(h4,21,22,24)key: idbiffksxlyuot-uhfffaoyaw smiles /n=c(/ccnc(=o)c1cc(cn1c)nc(=o)c2cc(cn2c)nc(=o)cn=c(n)n)\n properties chemical formula c18h26n10o3 · 2hcl molar mass 503.39 g/mol appearance white powder except where otherwise noted, data are given for materials in their standard state (at 25 °c , 100 kpa). n verify (what is yn ?) infobox references dna binding properties netropsin binds to the minor groove of at-rich sequences of double stranded dna. in contrast, netropsin does not bind single stranded dna or double stranded rna. crystallographic structures of dna-bound netropsin have been obtained and elucidate details of how the drug binds in the minor groove. in the bound structure, the drug makes hydrogen bonding interactions with four subsequent base pairs of the dna duplex, locally displacing the water molecules of the spine of hydration. netropsin (shown in space-filling representation) bound to dna (shown as bonds) using gel mobility and analytical ultracentrifugation, it was shown that netropsin binding to dna increases the twist per base by similar to 9˚ per molecule bound. thus, it removes supercoils when interacting with positively supercoiled dna and introduces (additional) negative supercoils when binding to relaxed or negatively supercoiled dna. netropsin's effect on supercoiled dna was observed in detail on single molecules using a magnetic tweezers. antibiotic properties it has been shown that netropsin is active both against gram-positive bacteria and gram-negative bacteria.