mycobacterium botniense mycobacterium botniense mycobacterium botniense is a slowly growing mycobacterium, which produces a yellow pigment. it was first isolated from a stream of water. m. botniense is most closely related to mycobacterium xenopi. etymology: botniense; of botnia, referring to the latin name of the province of finland from which the isolation was made. mycobacterium botniense scientific classification domain: bacteria phylum: actinomycetota class: actinomycetia order: mycobacteriales family: mycobacteriaceae genus: mycobacterium species: m. botniense binomial name mycobacterium botniensetorkko et al. 2000, atcc 700701 description microscopy gram-positive, nonmotile and acid-fast rods. colony characteristics colonies on löwenstein-jensen media and on middlebrook 7h11 agar are small, dysgonic and scotochromogenic, and produce yellow pigment. physiology visible growth from diluted inocula requires 5 to 8 weeks. growth occurs at 37 to 50 °c. the type strain is positive for 10-d arylsulfatase and pyrazinamidase. negative for 3-d arylsulfatase, urease, nitrate reductase, semi-quantitative catalase, heat-stable catalase, acid phosphatase, b-galactosidase and 5% nacl tolerance. tween 80 is not hydrolysed in 10 d. differential characteristics a phylogenetic tree based on the evaluation of 16s rdna sequences places m. botniense among the slow-growing mycobacteria, closest to m. xenopi. pathogenesis not known, but first isolated from an environmental source. type strain first isolated in finland from stream waters. strain e347 = atcc 700701 = ccug 47976 = cip 106753 = dsm 44537.