[header] How to perform an absorption based assay [title] Read the assay instruction sheet before starting the experiment. [substeps] Gather all materials and equipment needed to perform the experiment. [title] Plan out how much of each material will be needed to obtain the desired concentrations and which wells of the microplate to put it in. 
[substeps] Follow the instruction sheet in order to create the right mixtures of chemicals. [title] Add in the necessary reagents (enzymes, buffer solution, etc.