[header] How to denature a protein [title] Use heat. [step] Heat is one of the easiest ways and most common ways to denature a protein. When the protein in question is present in food, simply cooking the food will denature the proteins. 
Many proteins can be denatured by exposing them to a temperature of or above 100 ° c (212 ° f). This allows the proteins to coagulate and reduce their solubility.