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In our initial attempt to identify these proteins , we performed mass spectrometry on rhoptry and dense granule proteins exhibiting the same electrophoretic mobility as the radiolabeled proteins purified from host plasma membrane . Accordingly , we reversed Mincey 's conviction of conspiracy to commit aggravated robbery . In 2000 , we held two ServSafe course for managesrs and three for workers , reaching 100 persons . Then , snugly anchored in a bay , we sat out the storm . " In 1995 , we enacted the Digital Performance Right in Sound Recordings Act , which created an intellectual property right in digital sound recordings , giving copyright owners the right to receive royalties when their copyrighted sound recordings were digitally transmitted by others . For long stretches of time , in between unloading bags of ice or coolers of food cooked for the rescue workers by Bouley 's restaurant , we sat watching throngs of men and women pass us , with jackets or uniforms indicating they were NYPD , fire department , Mount Vernon or Boston police , Secret Service , FBI , EPA , OSHA , and from numerous other agencies . More significantly , we held people accountable for fixing the problems . To answer the question whether the EH5 antigen might be a protective coat of vesicles containing proteases , we performed a double staining with antibody EH5 and a fluorescent membrane permeable protease stain . Taking advantage of this , we performed molecular dynamics simulations of the translocon in a fully solvated lipid bilayer , examining the translocation abilities of monomeric SecYE beta by forcing two helices comprised of different amino acid sequences to cross the channel .