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Thus , an effort could be made to introduce standardized serum-free conditions for the growth of NTERA-2 and validate the reproducibility of its gene expression across laboratories in order to create an unlimited source of reference material for hESC . We examined the effects of THC on the replication of MHV68 and HVS , two rhadinoviruses related to KSHV and to lesser extent to EBV . Measures of identity and stability are generally of less concern to researchers growing hESCs than are the potency and undifferentiated state of the cells . This suggestion , for now , is based on the similar physical properties of PEP-CTERM and LPXTG transmembrane domains , the recurring association of EpsH genes with large exopolysaccharide biosynthesis loci , and the suggestion that the absolutely conserved residues Cys , Arg , and His found in the EpsH family are not merely fortuitously like the known catalytic triad His , Cys , and Arg ( a different order ) in the sortase family . To evaluate the presence of this connection , we determined by confocal immunocytochemistry the expression of ZO-1 and of F-actin , which associate with tight junction complexes .