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The missing variable , according to Robert Klitgaard , is the socio-cultural setting , which acts as the " symbolic soil " in which development takes place . As to the DePaul Clinic , it argued that two of the sample cases ( Federal Sample Nos . Counting House Trust 's principle activities involved opposing plans to construct a new Courthouse on the site of 50 South Main Street , and negotiating a purchase and sale agreement with the State of Rhode Island for this property . As she prepares for the season opener on Friday against North Carolina , a top-five team , Tucker says the No. 1 priority is getting healthy . The mutant enzyme , Ivy K308D , was cloned into a pet24a expression vector and transformed into BL21 cells .