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The biggest challenge was finding a way to collect water samples under the immense pressure of the deep sea , and then decompressing these samples so that they could be processed at normal atmospheric pressure inside the ESP 's robotic laboratory . The approach is hierarchical , beginning with the fitting of aliphatic groups ( methyl carbons , hydrogens , for instance ) , and then carrying these over into the determination of other groups . ENVIROTHON focuses on developing skills and understanding of the complexities of natural resource sciences and then putting these skills and concepts into action . When appropriate , we also fitted standard probability distributions to our data by using the method of maximum likelihood , in order to obtain parameter estimates , and then optimizing these estimates with the Levenberg-Marquardt algorithm .