[word=Glycosylation] [□]
[word=Glycosylation] [□ | □]
[word=Glycosylation] [□ & □]
Proteomics analysis of protein glycosylation : Glycosylation is the most common and complex form of posttranslational modification in eukaryotes . Glycosylation is the most frequent and varied of post-translational modifications , and yet it is the least-understood and most-poorly characterized because of the difficulty of glycoprotein production and the complexity of the oligosaccharide . Glycosylation consists of the addition of sugar groups ( usually oligosaccharides ) and can dramatically affect the three-dimensional structure and thus , function of a protein . Glycosylation is an important post-translational modification that influences protein function . ( Glycosylation is almost a given with these two conditions met . Glycosylation differs between species ; hence transgenic proteins are bound to exhibit differences in glycosylation patterns from the natural protein . Glycosylation is a complex post-translational event which can be influenced by a variety of factors , such as the culture conditions , the protein and carbohydrate supplements in the medium , and the purification procedures .