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I had students use Minitab to generate samples of sizes 10 , 30 , 100 , 300 , 1000 from a normal distribution . Prerequisites : junior or senior status , a 2.5 college G.P.A ., an average grade of 2.5 or higher in advanced writing courses taken ( English 245 , 265 , 300 , 355 ) , and permission of the English Department internship supervisor . Fasted tilapia were given 0 , 100 , 150 , 200 , 250 , 300 , or 350 mg / kg STZ iv . Severe hyperglycemia was seen in 20 , 80 , and 100 % of fish receiving 250 , 300 , and 350 mg / kg doses ; however , sections of islets showed only partial degranulation with no evidence of beta cell necrosis .