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( b ) The same shell partially decalcified . A total of 331 non-redundant ESTs were clustered using ClustalW and placed into one of five categories according to ( i ) the presence / absence of a signal sequence , whether they are ( ii ) a trafficking protein , ( iii ) a transcription factor , ( iv ) a signaling molecule or ( v ) have no identity with existing sequences in GenBank . ( c ) The mantle tissue that covers the majority of the dorsal surface of the animal is shaded grey . ( A ) The boxplots of Kendall 's rank correlation coefficient ( red ) show a weak anti-correlation between evolutionary rate and degree , which increases with subnet size . CLOE represents a simple and effective data mining approach that can be easily used for meta-analysis of cDNA microarray experiments characterized by very heterogeneous coverage . This dichotomy represents a demarcation between predominantly gray or white matter , the latter of which , as previously noted , is relatively poor in DHA . The choice of the most representative EST for the genes of interest represents a particularly critical aspect of our procedure . ( b ) The decalcified shell reveals a blue color within previous red fields ( * ) . In both cases , it retrieves a list of all probes found in the two datasets for the orthologous UniGene clusters . The average number of candidates produced by CLOE for each analyzed protein was approximately 17 , which represents a strong reduction if compared to the single organism approach ( 746 and 375 for the human and mouse datasets , respectively ) . In sum , our design could not determine the existence of a causal relationship between khat and psychosis – this would have been too ambitious a goal . The second is that , considering the widely different number of experiments and the relatively short phylogenetic distance between the two species , this represents a particularly severe test . As a control , we performed the same analysis on an equal number of randomly chosen ( and hence non-orthologous ) human-mouse EST pairs .