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In a typical structure of this type , most of the strand supply sources are mounted substantially co-axially within an elongated cradle , tube , cage , or flyer , and the strands are led to points away from the axis of such structure ( to clear other supply sources ) and thence along the structure toward a closing point which is beyond the confines of the flyer but located on the axis thereof . To test this , the team cloned rpsL PCR products containing the streptomycin-resistance marker into M13 bacteriophage " There was a marked favoritism for the double-strand DNA , " said Blaser , although the orientation of the clone did not play a role in the reaction in either the double strand or single strand case . However , long distance carriers have responded by drastically reducing corporate toll charges in an effort to maintain revenue and avoid stranded capacity on their installed networks . The term " twisting couple " as employed in this class defines that part of a spinning or twisting machine comprising a strand receiver and an element cooperating therewith to twist and wind the strand material .