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As a first proof of the method 's effectiveness , and in order to empirically determine a reasonable default cutoff for obtaining the final list of candidates , we analyzed whether genes with the highest ranks in the single organism lists are actually enriched of orthologous sequences . Besides being useful to other researchers to confirm the published results , the deposited datasets can be used as a substrate for new analysis , aimed at discovering functional modules by searching for related expression profiles . Public repositories of microarray data contain an incredible amount of information that is potentially relevant to explore functional relationships among genes by meta-analysis of expression profiles . Finally , to assess the redundancy of the information provided by the different probes , i.e. whether they represent different experiments and display similar / different expression profiles , the program calculates , for every pair of probes belonging to the same UniGene cluster , the number of common experiments and the Pearson correlation coefficient between their expression profiles . For example , the expression profiles of tumors reflect the transgenic oncogene [ 5 ] ; moreover , those tumors induced by genes that activate the Ras signaling pathway ( for example , polyoma middle T antigen , ErbB2 / Neu , and H-Ras ) are more similar to each other than to tumors induced by components of the Wnt signaling pathway ( Wnt1 , β - catenin , and c-Myc ) with respect both to gene expression patterns ( [ 5 ] , S. Huang et al ., in preparation ) and to cellular composition and histology [ 6-9 ] . In parallel , the human dataset was ranked for each human EST , and lists of candidates corresponding to the top 1 % ranks where obtained ( Single organism ) . Though useful in many cases to reduce the experimental noise , this procedure could result in a significant loss of information in the case of genes expressing different isoforms with distinct expression patterns [ 15 ] : on one hand the isoform-specific expression profiles would not be detected ; on the other hand , the average expression profile would be artificial and non-informative . The manner in which we determined medication gap size was based on periodic ( every 6 months ) and systematic reviews of medical records using an abstraction form developed for this study . Newspaper advertisements , daily radio programs and flyers informed the population about and helped them to understand the real purpose of the assessment ( at first rumors had spread that the research team would provide free medication for mentally disturbed individuals and created unreal expectations ) . Whiskers are the range .