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The posterior zone of the outer fold ( pzof ) , anterior zone of the outer fold ( azof ) , periostracal groove ( pg ) , inner fold ( if ) , and anterior edge of the outer fold ( aeof ) are indicated . Between the ventral most inner ( if ) and outer folds ( of ) lies the periostracal groove ( § ) . A cross section ( insert ) shows HasSom expression restricted to the dorsal surface of the outer fold ( white arrowhead ) , and to the dorsal surface of the periostracal groove ( black arrowhead ) . ( f ) Section through the mantle edge ( dorsal top , anterior right ) highlighting the periostracal groove ( § ) . Two of the six white matter regions investigated did not respond to treatments ( internal capsule and spinal cord [ includes white matter ] ) . In the present study , about 3 mm of gray matter was carefully removed from the underlying white corona radiation . In separate experiments , intracellular calcium stores were depleted by 1 μ M thapsigargin , which inhibits the Ca2 + ATPase pump of the endoplasmic reticulum . It has been shown from the analysis of multiple alignments and crystal structures that there is a high correlation between clusters of the most highly conserved residues in an alignment and functional sites ; this relationship has been observed to be strongest for enzyme catalytic sites , rather than protein-protein and protein-DNA interaction sites , and to apply best when the overall conservation observed in the alignment is low [ 38 ] . This dichotomy represents a demarcation between predominantly gray or white matter , the latter of which , as previously noted , is relatively poor in DHA . Although taxonomic filtering was used to minimize the bias introduced by the presence of multiple strains of the same species in the dataset , the resulting probabilities largely ignore the common ancestry of species with similar complements of proteins and should not be taken literally ; calculated " probabilities " are used only for comparison among different proteins from the same genome . Latent episomal DNA migrates much more slowly in these gels than linear replicating DNA [ 31,32 ] .