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After each cross back to maize inbreds , nearly all perennial attributes were lost , except for simple basal branching , and some vegetative persistence , but upon prolonged further generations of mass selection in isolations , perennialism could be restored , so that still another backcross to maize could be made . " Jury instructions must be viewed as a whole , and not in isolation . It [ Mathamma etc. ] should not be looked at in isolation . The creepiest thing about North Korea , for me , is not the nukes but the isolation - - it 's a factor that distinguishes NK strongly from the collapsing USSR . This section covers the biochemistry , physiology , pharmacology , toxicology , analysis , structure , properties , isolation , purification , tissue distribution , metabolism , and mechanisms of mammalian hormones , hormonal receptors , and hormone-like substances . Furthermore , the press shop has performed trials on press line compressed air isolations as part of their shutdown procedure . This is a highly dynamic area , at present , but tends to be done in isolation , hence , the integration of representations becomes an early issue . Other duties include working track-side on failures , derailments and other incidents including being responsible for electrical isolations . Embedded Best Practice process for access permits , plant isolations , tagout and clearance . In the absence of virus isolations and ( then unavailable ) genomic characterization it would be difficult to segregate the two .