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At CSC , we see RFID enabling the connection of the supply chain management ( SCM ) to the point-of-care . Using reverse transcriptase-polymerase chain reaction ( RT-PCR ) combined with nonisotopic RNase cleavage assay ( NIRCA ) , we found point mutations of the AML1 gene in 8 of 160 leukemia patients : silent mutations , heterozygous missense mutations , and biallelic nonsense or frameshift mutations in 2 , 4 , and 2 cases , respectively . METHODS : The cDNA of tomato profilin was amplified by reversely transcribed polymerase chain reaction ( RT-PCR ) from total RNA extracted from ripe tomato fruit . PCR detection should be used in combination with viral culture and immunocytologic and rapid antigen detection.A newly developed molecular diagnostic technique , multiplex reverse transcriptase-polymerase chain reaction ( MRT-PCR ) , permits rapid detection of RSV , adenoviruses , and PIV in appropriate respiratory tract secretions . Polymerase chain reaction ( PCR ) can identify specific translocations , such as t ( 11 ; 22 ) , in Ewing sarcoma and t ( 2 ; 13 ) in alveolar rhabdomyosarcoma , thus ruling out neuroblastoma.Neuroblastoma in bone marrow can be difficult to distinguish from other small , round , blue cell tumors of childhood.Histologic Findings : A biopsy usually is required to diagnose neuroblastoma . Therefore , antigen detection methods should be used in conjunction with cell culture for optimal diagnosis of viral infections.Gene amplificationPolymerase chain reaction ( PCR ) is a highly sensitive and specific technique for amplifying genes to detect the presence of a virus . NAA testing is essentially by polymerase chain reaction ( PCR ) techniques12 and has been progressively introduced in the different jurisdictions . Diagnosis is currently made by plasma activity assay for activated protein C ( APC ) resistance or polymerase chain reaction ( PCR ) - based DNA assay . The presence of immunoglobulin M antibodies indicates a recent infection.The diagnosis of Hantavirus infection is based on detection of Four Corners virus – specific antibody reactivities in serum and the detection of Four Corners virus ( FCV ) RNA in peripheral blood mononuclear cells by reverse transcriptase-polymerase chain reaction ( RT-PCR ) . Confronted with a highly competitive environment , supply chain management ( SCM ) is generally referred to as an effective means to help companies reduce costs , to be reactive and so on . Using oligonucleotide primers derived from the AML1 and ETO cDNAs , we were able to amplify a specific fusion transcript from 26 of 26 patients with t ( 8 ; 21 ) by a reverse transcriptase polymerase chain reaction ( PCR ) approach . Using reverse transcriptase-polymerase chain reaction ( RT-PCR ) followed by Southern blot , cloning , nucleotide sequencing and RT-in situ-PCR analyses , we have found that at least two CD44v3-containing isoforms , including one new species of CD44v2 , Dv3-10 ( Dv3 defined as a v3 exon lacking the first 24 base pairs ) and another previously reported CD44v3 ,8 -10 are preferentially expressed in human primary breast tumor and axillary nodal metastases but not in normal breast tissues . Commerce One 's Conductor helps companies speed the flow of such business processes as supply chain management ( SCM ) , customer relationship management ( CRM ) and enterprise resource planning ( ERP ) , making customer and partner interaction easier .