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Many of them demonstrated good activity against MurB in vitro and low MIC values against Gram-positive bacteria , particularly penicillin-resistant Streptococcus pneumoniae ( PRSP ) . There have been numerous reports of neurotransmitter perturbations in vitro and after short-term administration ( see Martin , 1986 ; Pertwee , 1988 ) . Michael examined the molecular composition of HLA-G in vitro and in vivo to determine if it occurred naturally in a form that would protect a fetus from a mother 's natural killer cells . To understand the relationship between env , microglial infection , and neurologic disease the Specific Aims of this proposal are ( 1 ) to identify the molecular features of CasBrE env which are responsible for receptor binding , amino acid transport inhibition , altered envelope processing , and defective viral replication in vitro and ( 2 ) to use the in vitro results to specifically reconstitute in the brain those viral replication events that are essential for inducing neurodegeneration .