tokens: The mutant receptors , as well as sBMPR-IA , were expressed as fusion proteins with thioredoxin in Escherichia coli , and purified using reverse phase high performance liquid chromatography ( RP-HPLC ) after digestion with enterokinase .
folded_tokens: the mutant receptor , as well as sbmpr-ia , be express as fusion protein with thioredoxin in escherichia coli , and purify use reverse phase high performance liquid chromatography ( rp-hplc ) after digestion with enterokinase .