tokens: As an application of reverse genetics , we introduced one nucleotide change ( UCU to ACU ) to immediate downstream of the RNA editing site of the V gene in the full-length hPIV2 cDNA and were able to obtain infectious viruses [ rPIV2V ( - ) ] from the cDNA .
folded_tokens: as a application of reverse genetics , we introduce one nucleotide change ( ucu to ACU ) to immediate downstream of the rna editing site of the v gene in the full-length hpiv2 cdna and be able to obtain infectious virus [ rpiv2v ( - ) ] from the cdna .