tokens: We have shown previously that GH3 cells transfected with the rat GnRH receptor cDNA ( GGH3-1 ' cells ) support the expression of a cotransfected fusion gene composed of 797 base pairs of rat LHbeta gene 5 ' - flanking sequence and the first 5 base pairs of the 5 ' - untranslated region fused to a luciferase reporter ( -797 / +5 LHbetaLUC ) and respond to a GnRH agonist with a 10-fold stimulation of activity .
folded_tokens: we have show previously that gh3 cell transfect with the rat gnrh receptor cdna ( ggh3-1 ' cell ) support the expression of a cotransfect fusion gene compose of 797 base pair of rat lhbeta gene 5 ' - flanking sequence and the first 5 base pair of the 5 ' - untranslated region fuse to a luciferase reporter ( -797 / +5 lhbetaluc ) and respond to a gnrh agonist with a 10-fold stimulation of activity .