tokens: Here we show that PKC and p44 / p42MAPK signalings are required for the HBx induced Sp1 mediated IGF-II P4 transcriptional activity since ( i ) PKC activation by PMA or PKC expression vector increases Sp1 phosphorylation and P4 activity in HBx transfected HepG2 cells ; ( ii ) PKC inhibition by PKC inhibitor Go6976 reduces Sp1 phosphorylation , P4 activity , and IGF-II mRNA in HBx transfected HepG2 cells ; and ( iii ) the inhibition of MEK activation by U0126 reduces Sp1 phosphorylation , P4 activity and IGF-II mRNA in HBx transfected HepG2 cells .
folded_tokens: here we show that pkc and p44 / p42mapk signaling be require for the hbx induce sp1 mediated igf-ii p4 transcriptional activity since ( i ) pkc activation by pma or pkc expression vector increase sp1 phosphorylation and p4 activity in hbx transfect hepg2 cell ; ( ii ) pkc inhibition by pkc inhibitor go6976 reduce sp1 phosphorylation , p4 activity , and igf-ii mrna in hbx transfect hepg2 cell ; and ( iii ) the inhibition of mek activation by u0126 reduce sp1 phosphorylation , p4 activity and igf-ii mrna in hbx transfect hepg2 cell .