tokens: Transient transfection assays using P19 cells revealed that expression of NDRF / NeuroD2 increased the transactivation of the rat insulin promoter element 3 ( RIPE3 ) enhancer up to approximately 12-fold and that co expression of catalytically active form of PKN , but not kinase deficient derivative , resulted in a further threefold increase of NDRF / NeuroD2 mediated transcription .
folded_tokens: transient transfection assay use p19 cell reveal that expression of ndrf / neurod2 increase the transactivation of the rat insulin promoter element 3 ( ripe3 ) enhancer up to approximately 12-fold and that co expression of catalytically active form of pkn , but not kinase deficient derivative , result in a further threefold increase of ndrf / neurod2 mediate transcription .