tokens: To investigate the convergent role of the insulin / IGF-I effector pathway mediating bihormonal stimulation of LDL receptor promoter expression , transfected granulosa-luteal cells were pretreated for 30 min with two specific inhibitors of phophatidylinositol 3-kinase , wortmannin ( 100 nM ) and LY 294002 ( 10 microM ) , or of mitogen activated protein kinase kinase , PD 98059 ( 50 microM ) , U0126 ( 10 microM ) , or the latter 's inactive derivative , U0124 ( 10 microM ) .
folded_tokens: to investigate the convergent role of the insulin / igf-i effector pathway mediate bihormonal stimulation of ldl receptor promoter expression , transfect granulosa-luteal cell be pretreat for 30 min with two specific inhibitor of phophatidylinositol 3-kinase , wortmannin ( 100 nm ) and ly 294002 ( 10 microm ) , or of mitogen activate protein kinase kinase , pd 98059 ( 50 microm ) , u0126 ( 10 microm ) , or the latter 's inactive derivative , u0124 ( 10 microm ) .