These studies have been extended to show that ( i ) a mutant EBV strain lacking the gene encoding EBNA 2 fails to switch from Wp to Cp usage in primary B lymphocytes , although the virus contains a functional Cp ; ( ii ) a region from -429 to -245 base pairs upstream of Cp is essential for Cp activity in B lymphocytes , but only in the context of upstream and downstream sequences ; ( iii ) this region contains an EBNA 2 -dependent enhancer ; and ( iv ) DNase I protection employing nuclear extracts from B and T lymphocytes revealed a B-cell-specific footprint in the region of the EBNA 2 -dependent enhancer .
these studies have been extended to show that ( i ) a mutant ebv strain lacking the gene encoding ebna 2 fails to switch from wp to cp usage in primary b lymphocytes , although the virus contains a functional cp ; ( ii ) a region from -429 to -245 base pairs upstream of cp is essential for cp activity in b lymphocytes , but only in the context of upstream and downstream sequences ; ( iii ) this region contains an ebna 2 -dependent enhancer ; and ( iv ) dnase i protection employing nuclear extracts from b and t lymphocytes revealed a b-cell-specific footprint in the region of the ebna 2 -dependent enhancer .
O O O O O O O O O O O O B-virus I-virus I-virus O O O O B-protein_molecule I-protein_molecule O O O O B-DNA_substructure O B-DNA_domain_or_region O O B-cell_type I-cell_type I-cell_type O O O O O O O B-DNA_domain_or_region O O O O O O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O B-DNA_domain_or_region O O O B-DNA_domain_or_region O O B-cell_type I-cell_type O O O O O O O B-DNA_domain_or_region O B-DNA_domain_or_region I-DNA_domain_or_region O O O O O O O O B-protein_molecule I-protein_molecule O O O O O O O B-protein_molecule I-protein_molecule O O B-cell_component I-cell_component O O O O O O O B-DNA_domain_or_region I-DNA_domain_or_region O O O O O B-protein_molecule I-protein_molecule O O O