Treatment with E2 or Prog increased survival and prevented apoptosis induced by TNF-alpha in both undifferentiated and macrophage-like PMA-differentiated U937 cells , as assessed by trypan blue exclusion cell counting , thymidine incorporation , AnnexinV labeling , followed by flow cytometry and DNA fragmentation studies .
treatment with e2 or prog increased survival and prevented apoptosis induced by tnf-alpha in both undifferentiated and macrophage-like pma-differentiated u937 cells , as assessed by trypan blue exclusion cell counting , thymidine incorporation , annexinv labeling , followed by flow cytometry and dna fragmentation studies .
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