Quantitative reverse transcriptase -polymerase chain reaction ( RT-PCR ) showed an increase in steady-state TNF-alpha mRNA after 3 to 4 hours of cross-linking .
quantitative reverse transcriptase -polymerase chain reaction ( rt-pcr ) showed an increase in steady-state tnf-alpha mrna after 3 to 4 hours of cross-linking .
O B-protein_molecule I-protein_molecule O O O O B-other_name O O O O O O B-protein_molecule O O O O O O O O O