We have developed a novel assay to monitor the active , GTP-bound form of Rap1 based on the differential affinity of Rap1GTP and Rap1GDP for the Rap binding domain of RalGDS ( RBD ) .
we have developed a novel assay to monitor the active , gtp-bound form of rap1 based on the differential affinity of rap1gtp and rap1gdp for the rap binding domain of ralgds ( rbd ) .
O O O O O O O O O O O B-protein_molecule I-protein_molecule O B-protein_molecule O O O O O O B-protein_molecule O B-protein_molecule O O B-protein_domain_or_region I-protein_domain_or_region I-protein_domain_or_region O B-protein_domain_or_region O B-protein_domain_or_region O O