Transient transfection of endothelial cells with ICAM-1 promoter luciferase reporter gene ( ICAM-1LUC ) constructs indicated that deletion of upstream NF-kappa B site ( -533 bases from translation start site ) had no effect on thrombin responsiveness , whereas mutation/deletion of downstream NF-kappa B site ( -223 bases from the translation start site ) prevented the activation of ICAM-1 promoter , indicating that the downstream NF-kappa B site is critical for thrombin inducibility .
transient transfection of endothelial cells with icam-1 promoter luciferase reporter gene ( icam-1luc ) constructs indicated that deletion of upstream nf-kappa b site ( -533 bases from translation start site ) had no effect on thrombin responsiveness , whereas mutation/deletion of downstream nf-kappa b site ( -223 bases from the translation start site ) prevented the activation of icam-1 promoter , indicating that the downstream nf-kappa b site is critical for thrombin inducibility .
O O O B-cell_type I-cell_type O B-protein_molecule O O O O O O O O O O O O O B-protein_complex I-protein_complex O O B-DNA_domain_or_region I-DNA_domain_or_region O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O O O O B-protein_molecule O O O O O O B-protein_complex I-protein_complex O O B-DNA_domain_or_region I-DNA_domain_or_region O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O O O O B-protein_molecule O O O O O O B-protein_complex I-protein_complex O O O O B-protein_molecule O O