Following stimulation , TNF-alpha was measured in cell supernatants by ELISA , NF-kappaB activity by electrophoretic mobility shift assay , and IkappaB-alpha levels by Western blot .
following stimulation , tnf-alpha was measured in cell supernatants by elisa , nf-kappab activity by electrophoretic mobility shift assay , and ikappab-alpha levels by western blot .
O O O B-protein_molecule O O O O O O B-other_name O B-protein_complex O O B-other_name I-other_name I-other_name I-other_name O O B-protein_molecule O O B-other_name I-other_name O