Transient cotransfection of tat cDNA in sense orientation ( tat/S ) , together with a plasmid containing the c-fos promoter ( FC3 , from- 711 to +42 ) in front of the bacterial chloramphenicol acetyltransferase ( CAT ) gene significantly enhanced CAT activity in Jurkat cells activated by the addition of 15% fetal calf serum ( FCS ) or 5 micrograms/mL phytohemagglutinin plus 10 ( -7 ) mol/L phorbol myristate acetate ( PMA ) and U937 cells activated by 15% FCS or 10 ( -7 ) mol/L PMA .
transient cotransfection of tat cdna in sense orientation ( tat/s ) , together with a plasmid containing the c-fos promoter ( fc3 , from- 711 to +42 ) in front of the bacterial chloramphenicol acetyltransferase ( cat ) gene significantly enhanced cat activity in jurkat cells activated by the addition of 15% fetal calf serum ( fcs ) or 5 micrograms/ml phytohemagglutinin plus 10 ( -7 ) mol/l phorbol myristate acetate ( pma ) and u937 cells activated by 15% fcs or 10 ( -7 ) mol/l pma .
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