To determine the mechanism of action of Ro 09-2210 , we set up a transient expression system in Jurkat T cells using a variety of reporter gene constructs and showed effective inhibition of phorbol ester / ionomycin -induced NF-AT activation and anti-CD3 induced NF-AT with IC50 = 7 . 7 and 10 nM , respectively .
to determine the mechanism of action of ro 09-2210 , we set up a transient expression system in jurkat t cells using a variety of reporter gene constructs and showed effective inhibition of phorbol ester / ionomycin -induced nf-at activation and anti-cd3 induced nf-at with ic50 = 7 . 7 and 10 nm , respectively .
O O O O O O O B-other_organic_compound I-other_organic_compound O O O O O O O O O B-cell_line I-cell_line I-cell_line O O O O O O O O O O O O B-other_organic_compound I-other_organic_compound O B-other_organic_compound O B-protein_family_or_group O O O O B-protein_family_or_group O O O O O O O O O O O O