When isolated PBMC were first cultured for 20 h in the presence of IL-10 on Teflon to prevent adherence , washed to remove IL-10 and then further cultured in plastic dishes for an additional 20 h in the presence of LPS or IL-1beta , an enhanced release of TNF was observed .
when isolated pbmc were first cultured for 20 h in the presence of il-10 on teflon to prevent adherence , washed to remove il-10 and then further cultured in plastic dishes for an additional 20 h in the presence of lps or il-1beta , an enhanced release of tnf was observed .
O O B-cell_type O O O O O O O O O O B-protein_molecule O B-inorganic O O O O O O O B-protein_molecule O O O O O O O O O O O O O O O O B-lipid O B-protein_molecule O O O O O B-protein_family_or_group O O O