Fragments of a 3 . 6 kb sequence of the human CRH gene promoter were amplified by PCR and ligated upstream of a CAT reporter .
fragments of a 3 . 6 kb sequence of the human crh gene promoter were amplified by pcr and ligated upstream of a cat reporter .
O O O O O O O O O O B-DNA_domain_or_region B-protein_molecule O O O O O O O O O O O B-DNA_family_or_group I-DNA_family_or_group O