Using the technique of reverse transcription-polymerase chain reaction ( PCR ) with RNA isolated from lymphoid cell lines of the patient and of controls , no evidence was found that IVS1-10TC abnormally disrupted mRNA splicing or caused the absence of BRCA1 mRNA .
using the technique of reverse transcription-polymerase chain reaction ( pcr ) with rna isolated from lymphoid cell lines of the patient and of controls , no evidence was found that ivs1-10tc abnormally disrupted mrna splicing or caused the absence of brca1 mrna .
O O O O B-other_name I-other_name I-other_name I-other_name O B-other_name O O O O O B-cell_line I-cell_line I-cell_line O O O O O O O O O O O O B-other_name I-other_name O B-RNA_family_or_group O O O O O O B-DNA_domain_or_region O O