Three days after establishing high-density PBMC cultures ( 1 . 5 x 10 ( 6 ) cells/cm2 ) , in serum-containing medium , small adherent colonies of tartrate resistant acid phosphatase positive ( TRAP+ ) cells emerge , amidst massive monocyte cell death .
three days after establishing high-density pbmc cultures ( 1 . 5 x 10 ( 6 ) cells/cm2 ) , in serum-containing medium , small adherent colonies of tartrate resistant acid phosphatase positive ( trap+ ) cells emerge , amidst massive monocyte cell death .
O O O O B-other_name B-cell_type O O O O O O O O O O O O O O O O O O B-cell_line I-cell_line O B-cell_line I-cell_line I-cell_line I-cell_line I-cell_line I-cell_line I-cell_line I-cell_line I-cell_line O O O B-other_name I-other_name I-other_name I-other_name O