The best medium of those tested was RPMI 1640 supplemented with 5 micrograms/ml each transferrin and insulin + 5 ng/ml sodium selenite +/- 0 . 1% bovine serum albumin .
the best medium of those tested was rpmi 1640 supplemented with 5 micrograms/ml each transferrin and insulin + 5 ng/ml sodium selenite +/- 0 . 1% bovine serum albumin .
O O O O O O O O O O O O O O B-protein_molecule O B-protein_molecule O O O B-other_organic_compound I-other_organic_compound O O O O B-protein_molecule I-protein_molecule I-protein_molecule O