By using a partial nucleotide sequence from the cloned TSC2 and polymerase chain reaction methodology , we constructed a digoxigenin-labeled complementary DNA probe to examine TSC2 gene expression in autopsy- or biopsy-derived human tissues by in situ hybridization .
by using a partial nucleotide sequence from the cloned tsc2 and polymerase chain reaction methodology , we constructed a digoxigenin-labeled complementary dna probe to examine tsc2 gene expression in autopsy- or biopsy-derived human tissues by in situ hybridization .
O O O B-DNA_N/A I-DNA_N/A I-DNA_N/A O O O B-DNA_domain_or_region O B-other_name I-other_name I-other_name I-other_name O O O O B-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule O O B-DNA_domain_or_region O O O O O O B-tissue I-tissue O B-other_name I-other_name I-other_name O