In addition , two mutations between -93 and -76 and between -75 and -58 were utilized , since they affect the nuclear factor kappa B ( NF-kappa B ) - and Sp1 -binding sites and were expected to diminish viral replication .
in addition , two mutations between -93 and -76 and between -75 and -58 were utilized , since they affect the nuclear factor kappa b ( nf-kappa b ) - and sp1 -binding sites and were expected to diminish viral replication .
O O O O O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O O O O O O B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule O B-protein_molecule I-protein_molecule O O O B-protein_molecule O O O O O O O O O O