To determine whether Nef affects the binding of cellular factors implicated in HIV-1 regulation , 32P-labeled oligonucleotides corresponding to the binding sites were incubated with nuclear extracts prepared from Nef-expressing T-cell lines that were not stimulated or were stimulated with T-cell mitogens .
to determine whether nef affects the binding of cellular factors implicated in hiv-1 regulation , 32p-labeled oligonucleotides corresponding to the binding sites were incubated with nuclear extracts prepared from nef-expressing t-cell lines that were not stimulated or were stimulated with t-cell mitogens .
O O O B-protein_family_or_group O O O O B-protein_family_or_group I-protein_family_or_group O O B-other_name I-other_name O B-polynucleotide I-polynucleotide O O O O O O O O B-cell_component I-cell_component O O B-cell_line I-cell_line I-cell_line O O O O O O O O B-protein_family_or_group I-protein_family_or_group O