This effect was specifically due to Tat , since Jurkat and U937 cells cotransfected either with tat cDNA in antisense orientation ( tat/AS ) , tat carrying a mutation in the aminoacid cys22-gly22 ( tat 22/S ) or with the backbone vector alone ( pRPneo-SL3 ) did not show any significant difference in c-fos promoter activity as compared to cells transfected with FC3 plasmid alone .
this effect was specifically due to tat , since jurkat and u937 cells cotransfected either with tat cdna in antisense orientation ( tat/as ) , tat carrying a mutation in the aminoacid cys22-gly22 ( tat 22/s ) or with the backbone vector alone ( prpneo-sl3 ) did not show any significant difference in c-fos promoter activity as compared to cells transfected with fc3 plasmid alone .
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