We overexpressed in the Jurkat T-cell line ZAP-70 mutated on Tyr-492 and Tyr-493 in the putative regulatory loop of its kinase domain .
we overexpressed in the jurkat t-cell line zap-70 mutated on tyr-492 and tyr-493 in the putative regulatory loop of its kinase domain .
O O O O B-cell_line I-cell_line I-cell_line B-protein_molecule O O B-amino_acid_monomer O B-amino_acid_monomer O O O O O O O B-protein_domain_or_region I-protein_domain_or_region O