Using magnetic beads with attached DNA containing the Escherichia coli lac operator , fusion proteins comprising the DNA-binding lac repressor could be affinity-purified and recovered by gentle elution conditions , such as with a lactose analogue or by enzymatic means using either deoxyribonuclease ( DNase ) or restriction endonucleases .
using magnetic beads with attached dna containing the escherichia coli lac operator , fusion proteins comprising the dna-binding lac repressor could be affinity-purified and recovered by gentle elution conditions , such as with a lactose analogue or by enzymatic means using either deoxyribonuclease ( dnase ) or restriction endonucleases .
O B-inorganic I-inorganic O O B-DNA_N/A O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O B-protein_family_or_group I-protein_family_or_group O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O O O O O O O O O O O O O B-carbohydrate I-carbohydrate O O O O O O B-protein_molecule O B-protein_molecule O O B-protein_molecule I-protein_molecule O