Results from in vivo expression studies performed with these NF-kappa B p65 mutants revealed the following : 1 ) I kappa B/MAD-3 completely inhibits NF-kappa B p65 -dependent transcriptional activation mediated through the human immunodeficiency virus type 1 kappa B enhancer in human T lymphocytes , 2 ) the binding of I kappa B/MAD-3 to NF-kappa B p65 is sufficient to retarget NF-kappa B p65 from the nucleus to the cytoplasm , 3 ) selective deletion of the functional nuclear localization signal present in the Rel homology domain of NF-kappa B p65 disrupts its ability to engage I kappa B/MAD-3 , and 4 ) the unique C-terminus of NF-kappa B p65 attenuates its own nuclear localization and contains sequences that are required for I kappa B -mediated inhibition of NF-kappa B p65 DNA binding activity .
results from in vivo expression studies performed with these nf-kappa b p65 mutants revealed the following : 1 ) i kappa b/mad-3 completely inhibits nf-kappa b p65 -dependent transcriptional activation mediated through the human immunodeficiency virus type 1 kappa b enhancer in human t lymphocytes , 2 ) the binding of i kappa b/mad-3 to nf-kappa b p65 is sufficient to retarget nf-kappa b p65 from the nucleus to the cytoplasm , 3 ) selective deletion of the functional nuclear localization signal present in the rel homology domain of nf-kappa b p65 disrupts its ability to engage i kappa b/mad-3 , and 4 ) the unique c-terminus of nf-kappa b p65 attenuates its own nuclear localization and contains sequences that are required for i kappa b -mediated inhibition of nf-kappa b p65 dna binding activity .
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