Second , by high stringency Northern blotting and in situ hybridization , TCF-1 expression was shown to be confined to the thymus and to the T cell areas of the spleen .
second , by high stringency northern blotting and in situ hybridization , tcf-1 expression was shown to be confined to the thymus and to the t cell areas of the spleen .
O O O B-other_name I-other_name I-other_name I-other_name O B-other_name I-other_name I-other_name O B-protein_molecule O O O O O O O O B-body_part O O O O O O O O B-body_part O