To determine whether the functional defect of the patients' T cells was due to the absence or abnormal binding of transcription factors involved in cytokine gene expression , electrophoretic mobility shift assays were used to examine the DNA binding of AP-1 , Oct , CREB , SP1 , NF-kappa B and the nuclear factor of activated T cells ( NF-AT ) to their respective response elements in the promoter of the IL-2 gene .
to determine whether the functional defect of the patients' t cells was due to the absence or abnormal binding of transcription factors involved in cytokine gene expression , electrophoretic mobility shift assays were used to examine the dna binding of ap-1 , oct , creb , sp1 , nf-kappa b and the nuclear factor of activated t cells ( nf-at ) to their respective response elements in the promoter of the il-2 gene .
O O O O O O O O O B-cell_type I-cell_type O O O O O O O O O B-protein_family_or_group I-protein_family_or_group O O B-DNA_family_or_group I-DNA_family_or_group O O B-other_name I-other_name I-other_name I-other_name O O O O O O O O B-protein_molecule O B-protein_family_or_group O B-protein_family_or_group O B-protein_molecule O B-protein_complex I-protein_complex O O B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule B-cell_type I-cell_type O B-protein_molecule O O O O B-DNA_domain_or_region I-DNA_domain_or_region O O B-DNA_domain_or_region O O B-protein_molecule O O