Using the reverse transcription-polymerase chain reaction ( RT ) assay , the limit of detection was 10 ( -5 ) to 10 ( -6 ) for PML/RAR alpha transcript and 10 ( -4 ) to 10 ( -5 ) for the AML1/ETO transcript .
using the reverse transcription-polymerase chain reaction ( rt ) assay , the limit of detection was 10 ( -5 ) to 10 ( -6 ) for pml/rar alpha transcript and 10 ( -4 ) to 10 ( -5 ) for the aml1/eto transcript .
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