METHODS : Protein production in supernatants was assessed by ELISA , and mRNA expression was evaluated by semiquantitative RT- PCR .
methods : protein production in supernatants was assessed by elisa , and mrna expression was evaluated by semiquantitative rt- pcr .
O O B-other_name I-other_name O O O O O B-other_name O O B-RNA_family_or_group O O O O O O B-other_name O