In U-937 cells , optimum time and dose of pretreatment with 1 , 25- ( OH ) 2D3 were determined to be 12-24 h at a receptor saturating concentration of 1 , 25- ( OH ) 2D3 ( 10 nM ) .
in u-937 cells , optimum time and dose of pretreatment with 1 , 25- ( oh ) 2d3 were determined to be 12-24 h at a receptor saturating concentration of 1 , 25- ( oh ) 2d3 ( 10 nm ) .
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