To further explore the involvement of PI3-K in T cell activation , we created a set of potentially dominant negative PI3-K constructs comprising individual or tandem domains of the regulatory p85 subunit and tested their effect on downstream signaling events like Erk2 activation and transcription from an NFAT ( nuclear factor of activated T cells ) element taken from the interleukin-2 promoter .
to further explore the involvement of pi3-k in t cell activation , we created a set of potentially dominant negative pi3-k constructs comprising individual or tandem domains of the regulatory p85 subunit and tested their effect on downstream signaling events like erk2 activation and transcription from an nfat ( nuclear factor of activated t cells ) element taken from the interleukin-2 promoter .
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