When the wild-type hGR and hGR -Ile 729 were expressed in COS-1 cells and assayed for [ 3H ] -Dexamethasone binding , the dissociation constants were 0 . 799 +/- 0 . 068 and 1 . 54 +/- 0 . 06 nM ( mean +/- SEM ) ( P < 0 . 01 ) , respectively .
when the wild-type hgr and hgr -ile 729 were expressed in cos-1 cells and assayed for [ 3h ] -dexamethasone binding , the dissociation constants were 0 . 799 +/- 0 . 068 and 1 . 54 +/- 0 . 06 nm ( mean +/- sem ) ( p < 0 . 01 ) , respectively .
O O B-protein_molecule B-protein_family_or_group O B-protein_family_or_group O O O O O B-cell_line I-cell_line O O O B-lipid I-lipid I-lipid I-lipid O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O