To investigate the molecular mechanism of the inhibitory effect on transcriptional or post-transcriptional regulation of IL-6 gene expression by IL-4 and IL-10 , we studied IL-6 production , expression level of IL-6 mRNA , IL-6 promoter activity , transcriptional activity of NF-kappaB and NF- IL-6 , and IL-6 mRNA stability in human monocytic cell lines , THP-1 and U937 , stimulated by PMA and LPS in the absence or the presence of IL-4 or IL-10 .
to investigate the molecular mechanism of the inhibitory effect on transcriptional or post-transcriptional regulation of il-6 gene expression by il-4 and il-10 , we studied il-6 production , expression level of il-6 mrna , il-6 promoter activity , transcriptional activity of nf-kappab and nf- il-6 , and il-6 mrna stability in human monocytic cell lines , thp-1 and u937 , stimulated by pma and lps in the absence or the presence of il-4 or il-10 .
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