This hybrid clone , designated NR2 , was characterized by several methods , including PCR , with eight pairs of oligonucleotides mapped to Chr 20 : D20S5 , D20S41 , D20S42 , D20S56 , D20S57 , D20S58 , adenosine deaminase ( ADA ) , and Prion protein ( PRIP ) ; Restriction Fragment Length Polymorphism ( RFLP ) analyses with four genomic anonymous probes ( D20S5 , cD3H12 , D20S17 , D20S18 ) ; and fluorescent in situ hybridization ( FISH ) with total human DNA and D20Z1 , a sequence specific to the human Chr 20 centromere , as probes .
this hybrid clone , designated nr2 , was characterized by several methods , including pcr , with eight pairs of oligonucleotides mapped to chr 20 : d20s5 , d20s41 , d20s42 , d20s56 , d20s57 , d20s58 , adenosine deaminase ( ada ) , and prion protein ( prip ) ; restriction fragment length polymorphism ( rflp ) analyses with four genomic anonymous probes ( d20s5 , cd3h12 , d20s17 , d20s18 ) ; and fluorescent in situ hybridization ( fish ) with total human dna and d20z1 , a sequence specific to the human chr 20 centromere , as probes .
O O O O O B-cell_line O O O O O O O O B-other_name O O O O O B-polynucleotide O O B-DNA_molecule I-DNA_molecule O B-DNA_domain_or_region O B-DNA_domain_or_region O B-DNA_domain_or_region O B-DNA_domain_or_region O B-DNA_domain_or_region O B-DNA_domain_or_region O B-protein_molecule I-protein_molecule O B-protein_molecule O O O B-protein_molecule I-protein_molecule O B-protein_molecule O O B-other_name I-other_name I-other_name I-other_name I-other_name B-DNA_domain_or_region O O O O B-DNA_family_or_group I-DNA_family_or_group I-DNA_family_or_group O B-DNA_molecule O B-DNA_molecule O B-DNA_molecule O B-DNA_family_or_group O O O B-other_name I-other_name I-other_name I-other_name O B-other_name O O B-DNA_family_or_group I-DNA_family_or_group I-DNA_family_or_group O B-DNA_domain_or_region O O O O O O B-DNA_domain_or_region B-DNA_molecule I-DNA_molecule O O O O O