Using electrophoretic mobility shift assays ( EMSA ) , we now show that , in monocytes stimulated with LPS or TNF alpha , IL-10 inhibits nuclear stimulation of nuclear factor kappa B ( NF kappa B ) , a transcription factor involved in the expression of inflammatory cytokine genes . Several other transcription factors including NF- IL-6 , AP-1 , AP-2 , GR , CREB , Oct-1 , and Sp-1 are not affected by IL-10 .
using electrophoretic mobility shift assays ( emsa ) , we now show that , in monocytes stimulated with lps or tnf alpha , il-10 inhibits nuclear stimulation of nuclear factor kappa b ( nf kappa b ) , a transcription factor involved in the expression of inflammatory cytokine genes . several other transcription factors including nf- il-6 , ap-1 , ap-2 , gr , creb , oct-1 , and sp-1 are not affected by il-10 .
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