Our studies demonstrate that incubation of human T cells with low concentrations ( approximately 1-5 nM ) of calyculin A or okadaic acid , potent inhibitors of protein phosphatase type 1 ( PP-1 ) and type 2A ( PP-2A ) , induces the phosphorylation of I kappa B alpha even in the absence of any cellular stimulus .
our studies demonstrate that incubation of human t cells with low concentrations ( approximately 1-5 nm ) of calyculin a or okadaic acid , potent inhibitors of protein phosphatase type 1 ( pp-1 ) and type 2a ( pp-2a ) , induces the phosphorylation of i kappa b alpha even in the absence of any cellular stimulus .
O O O O O O B-cell_type I-cell_type I-cell_type O O O O O O O O O B-other_organic_compound I-other_organic_compound O B-other_organic_compound I-other_organic_compound O O O O O O O O O O O O O O O B-protein_family_or_group O O O O B-other_name O B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule O O O O O O O O O