METHODS AND RESULTS : Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours , and secretion of MCP-1 and surface expression of ICAM-1 were determined by ELISA and flow cytometry , respectively .
methods and results : monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or adp-activated platelets for 6 hours , and secretion of mcp-1 and surface expression of icam-1 were determined by elisa and flow cytometry , respectively .
O O O O B-other_name O B-cell_type I-cell_type I-cell_type B-cell_type I-cell_type O O O O O B-cell_type I-cell_type O O O O O O O B-protein_molecule O B-other_name I-other_name O B-protein_molecule O O O B-other_name O B-other_name I-other_name O O O