For this purpose , we transiently transfected Jurkat T cells with the reporter plasmid p [ ( TRE ) 3-tk-Luc ] and found that CCK-8 was able to dose-dependently induce luciferase expression related to activator protein-1 ( AP-1 ) activation with a maximal response identical to that obtained with compounds known to activate AP-1 complex ( quantitatively , the same level of induction was obtained with 1 nM 12-O-tetradecanoylphorbol-13-acetate , 100 microM diacylglycerol , or 4 nM epidermal growth factor ) .
for this purpose , we transiently transfected jurkat t cells with the reporter plasmid p [ ( tre ) 3-tk-luc ] and found that cck-8 was able to dose-dependently induce luciferase expression related to activator protein-1 ( ap-1 ) activation with a maximal response identical to that obtained with compounds known to activate ap-1 complex ( quantitatively , the same level of induction was obtained with 1 nm 12-o-tetradecanoylphorbol-13-acetate , 100 microm diacylglycerol , or 4 nm epidermal growth factor ) .
O O O O O O O B-cell_line I-cell_line I-cell_line O O B-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule O O O B-peptide O O O O O B-protein_molecule O O O B-protein_molecule I-protein_molecule O B-protein_molecule O O O O O O O O O O O O O O O B-protein_molecule O O O O O O O O O O O O O O B-other_organic_compound O O O B-other_organic_compound O O O O B-other_organic_compound I-other_organic_compound I-other_organic_compound O O