In contrast to freshly isolated T cells , in vitro-cultured T cells did not require costimulation for cytokine secretion in response to anti-CD3 alone .
in contrast to freshly isolated t cells , in vitro-cultured t cells did not require costimulation for cytokine secretion in response to anti-cd3 alone .
O O O B-cell_type I-cell_type I-cell_type I-cell_type O O B-cell_line I-cell_line I-cell_line O O O O O B-protein_family_or_group O O O O B-protein_molecule O O