As NF-kappa B is a critical transcription factor in the control of IL-2 expression , we next analysed its nuclear translocation in neonatal and adult T cells using the electrophoretic mobility shift assay and , because induction of reactive oxygen intermediates ( ROI ) is required for the activation of NF-kappa B , we also analysed levels of intracellular ROI in these cells using the ROI -reactive fluorochrome DCFH-DA and flow cytometry .
as nf-kappa b is a critical transcription factor in the control of il-2 expression , we next analysed its nuclear translocation in neonatal and adult t cells using the electrophoretic mobility shift assay and , because induction of reactive oxygen intermediates ( roi ) is required for the activation of nf-kappa b , we also analysed levels of intracellular roi in these cells using the roi -reactive fluorochrome dcfh-da and flow cytometry .
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