Using site-directed mutagenesis , we found that two of these predicted alpha-helices are required for full transcriptional activation by CIITA .
using site-directed mutagenesis , we found that two of these predicted alpha-helices are required for full transcriptional activation by ciita .
O O O O O O O O O O O B-protein_substructure O O O O B-other_name I-other_name O B-protein_molecule O