All conserved cysteine and arginine residues in this domain were mutated by site-directed mutagenesis , and the mutant proteins were characterized by blocking reactions , ligand-binding experiments , transactivation assays , and protease mapping .
all conserved cysteine and arginine residues in this domain were mutated by site-directed mutagenesis , and the mutant proteins were characterized by blocking reactions , ligand-binding experiments , transactivation assays , and protease mapping .
O O B-amino_acid_monomer O B-amino_acid_monomer I-amino_acid_monomer O O O O B-other_name I-other_name I-other_name I-other_name O O O B-protein_family_or_group I-protein_family_or_group O O O B-other_name I-other_name O B-other_name I-other_name O B-other_name I-other_name O O B-protein_family_or_group O O