We describe a method for relative quantification of specific mRNA using a nonradioactive assay based on DNA strand competition between identical sequences of biotin- and fluorescein-labeled amplicon ( probe ) and unlabeled amplicon ( target ) during hybridization .
we describe a method for relative quantification of specific mrna using a nonradioactive assay based on dna strand competition between identical sequences of biotin- and fluorescein-labeled amplicon ( probe ) and unlabeled amplicon ( target ) during hybridization .
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