In previous transfection analyses using the chloramphenicol acetyltransferase reporter gene system , we determined that linker substitution ( LS ) mutations between -201 and -130 ( relative to the transcription start site ) of the human immunodeficiency virus type 1 long terminal repeat ( LTR ) caused moderate decreases in LTR transcriptional activity in a T-cell line ( S . L . Zeichner , J . Y . H . Kim , and J . C . Alwine , J . Virol . 65 : 2436-2444 , 1991 ) .
in previous transfection analyses using the chloramphenicol acetyltransferase reporter gene system , we determined that linker substitution ( ls ) mutations between -201 and -130 ( relative to the transcription start site ) of the human immunodeficiency virus type 1 long terminal repeat ( ltr ) caused moderate decreases in ltr transcriptional activity in a t-cell line ( s . l . zeichner , j . y . h . kim , and j . c . alwine , j . virol . 65 : 2436-2444 , 1991 ) .
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