To establish a model for 1 , 25- ( OH ) 2D3 regulation of human monocyte monokine synthesis , three human cell lines ( U-937 , THP-1 , and HL-60 ) were examined for : 1 ) the presence of functional 1 , 25- ( OH ) 2D3 receptors ; 2 ) the accumulation of interleukin-1 beta ( IL-1 beta ) mRNA and IL-1 beta protein in response to lipopolysaccharide ( LPS ) ; and 3 ) the regulation of this response by 1 , 25- ( OH ) 2D3 .
to establish a model for 1 , 25- ( oh ) 2d3 regulation of human monocyte monokine synthesis , three human cell lines ( u-937 , thp-1 , and hl-60 ) were examined for : 1 ) the presence of functional 1 , 25- ( oh ) 2d3 receptors ; 2 ) the accumulation of interleukin-1 beta ( il-1 beta ) mrna and il-1 beta protein in response to lipopolysaccharide ( lps ) ; and 3 ) the regulation of this response by 1 , 25- ( oh ) 2d3 .
O O O O O B-lipid I-lipid I-lipid I-lipid I-lipid I-lipid I-lipid O O B-other_name I-other_name I-other_name I-other_name O O B-cell_line I-cell_line I-cell_line O B-cell_line O B-cell_line O O B-cell_line O O O O O O O O O O O B-lipid I-lipid I-lipid I-lipid I-lipid I-lipid I-lipid O O O O O O O B-protein_molecule I-protein_molecule O B-protein_molecule I-protein_molecule O O O B-protein_molecule I-protein_molecule O O O O B-lipid O B-lipid O O O O O O O O O O O B-lipid I-lipid I-lipid I-lipid I-lipid I-lipid I-lipid O