The levels of cAMP-dependent protein kinase and Rap1 were assessed by Western blot analysis , immunostaining , and computer-assisted imaging .
the levels of camp-dependent protein kinase and rap1 were assessed by western blot analysis , immunostaining , and computer-assisted imaging .
O O O B-protein_family_or_group I-protein_family_or_group I-protein_family_or_group O B-protein_molecule O O O O O O O O O O B-other_name I-other_name O