Addition of the -176 to -96 sequence to the 4X NF-AT-Oct construct did not impart the ability to be affected by anergy , but addition of the -236 to -96 sequence did , demonstrating that anergy is an active inhibitory process and that more than the presence of the -150 AP-1 binding site ( -152 to -147 ) is required to mediate the effect .
addition of the -176 to -96 sequence to the 4x nf-at-oct construct did not impart the ability to be affected by anergy , but addition of the -236 to -96 sequence did , demonstrating that anergy is an active inhibitory process and that more than the presence of the -150 ap-1 binding site ( -152 to -147 ) is required to mediate the effect .
O O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O B-DNA_domain_or_region I-DNA_domain_or_region O O O O O O O O O O O O O O O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O O O O O O O O O O O O O O O O O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O B-DNA_domain_or_region I-DNA_domain_or_region I-DNA_domain_or_region O O O O O O O O