After the DNA fragment containing the CAG repeats is amplified by the polymerase chain reaction , a primer extension is carried out ; the extension of the end-labelled reverse primer adjacent to 3' end of CAG repeats stops at the first T after CAG repeats with the incorporation of dideoxy ATP in the reaction mixture .
after the dna fragment containing the cag repeats is amplified by the polymerase chain reaction , a primer extension is carried out ; the extension of the end-labelled reverse primer adjacent to 3' end of cag repeats stops at the first t after cag repeats with the incorporation of dideoxy atp in the reaction mixture .
O O B-DNA_molecule I-DNA_molecule O O B-DNA_domain_or_region I-DNA_domain_or_region O O O O B-other_name I-other_name I-other_name O O B-other_name I-other_name O O O O O O O O B-DNA_molecule I-DNA_molecule I-DNA_molecule O O B-DNA_domain_or_region I-DNA_domain_or_region O B-DNA_domain_or_region I-DNA_domain_or_region O O O O B-nucleotide O B-DNA_domain_or_region I-DNA_domain_or_region O O O O B-nucleotide I-nucleotide O O O O O