DHEA binding sites were examined using a whole-cell binding assay in a human T lymphoid cell line , PEER , revealing that a single class of high-affinity binding sites for DHEA ( dissociation constant = 7 . 4 +/- 0 . 53 nmol/L , mean +/- SE , n = 4 ) was greatly increased when treated with DHEA , phorbol-12-myristate-13-acetate , and the Ca2+ ionophore A23187 .
dhea binding sites were examined using a whole-cell binding assay in a human t lymphoid cell line , peer , revealing that a single class of high-affinity binding sites for dhea ( dissociation constant = 7 . 4 +/- 0 . 53 nmol/l , mean +/- se , n = 4 ) was greatly increased when treated with dhea , phorbol-12-myristate-13-acetate , and the ca2+ ionophore a23187 .
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