Our results demonstrated that : ( i ) PGN induced phosphorylation of the transcription factors ATF-1 and CREB ; ( ii ) ATF-1 and CREB bound DNA as a dimer and induced transcriptional activation of a CRE reporter plasmid , which was inhibited by dominant negative CREB and ATF-1 ; ( iii ) PGN induced phosphorylation of c-Jun , protein synthesis of JunB and c-Fos , and transcriptional activation of the AP-1 reporter plasmid , which was inhibited by dominant negative c-Fos ; and ( iv ) PGN -induced activation of CREB /ATF and AP-1 was mediated through CD14 .
our results demonstrated that : ( i ) pgn induced phosphorylation of the transcription factors atf-1 and creb ; ( ii ) atf-1 and creb bound dna as a dimer and induced transcriptional activation of a cre reporter plasmid , which was inhibited by dominant negative creb and atf-1 ; ( iii ) pgn induced phosphorylation of c-jun , protein synthesis of junb and c-fos , and transcriptional activation of the ap-1 reporter plasmid , which was inhibited by dominant negative c-fos ; and ( iv ) pgn -induced activation of creb /atf and ap-1 was mediated through cd14 .
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