We now show that : 1 ) CVZ has more than one binding site in a second , independent , B-cell line , IM-9 ; 2 ) the antiglucocorticoid RU 38486 is able to block both CVZ 's higher and lower affinity sites ; 3 ) all of CVZ 's binding sites are on a protein immunologically indistinguishable from the human GR ; and 4 ) freshly isolated clones of CVZ -resistant cells have lost all binding sites for CVZ .
we now show that : 1 ) cvz has more than one binding site in a second , independent , b-cell line , im-9 ; 2 ) the antiglucocorticoid ru 38486 is able to block both cvz 's higher and lower affinity sites ; 3 ) all of cvz 's binding sites are on a protein immunologically indistinguishable from the human gr ; and 4 ) freshly isolated clones of cvz -resistant cells have lost all binding sites for cvz .
O O O O O O O B-other_organic_compound O O O O O O O O O O O O B-cell_line I-cell_line O B-cell_line O O O O B-other_name B-other_organic_compound I-other_organic_compound O O O O O B-other_organic_compound O O O O B-other_name O O O O O O B-other_organic_compound O O O O O O O O O O O O B-protein_family_or_group O O O O O O O O B-other_organic_compound O O O O O O O O B-other_organic_compound O