Peripheral blood monocytes were isolated by density centrifugation methods with a purity of >90% and were cultured in RPMI 1640 medium .
peripheral blood monocytes were isolated by density centrifugation methods with a purity of >90% and were cultured in rpmi 1640 medium .
B-cell_type I-cell_type I-cell_type O O O O O O O O O O O O O O O O O O O