Using a recently developed transfection technique , we demonstrate that in primary human lymphocytes , stimulation of a 548 bp IL-2 promoter -luciferase reporter construct by phorbol ester and calcium ionophore is reversed by dexamethasone to a similar extent as in Jurkat T lymphoma cells transfected with a GRalpha expression vector .
using a recently developed transfection technique , we demonstrate that in primary human lymphocytes , stimulation of a 548 bp il-2 promoter -luciferase reporter construct by phorbol ester and calcium ionophore is reversed by dexamethasone to a similar extent as in jurkat t lymphoma cells transfected with a gralpha expression vector .
O O O O O O O O O O O B-cell_type I-cell_type I-cell_type O O O O B-DNA_molecule I-DNA_molecule B-DNA_domain_or_region I-DNA_domain_or_region B-protein_molecule O O O O O O O O O O O O O O O O O O O O O O O O O B-protein_molecule O O O