Despite this drastic effect in vivo , the in vitro immortalization of erythropoietin-dependent erythroid progenitors unexpectedly occurred at low frequency , and all four cell lines established expressed both erythrocytic ( globins ) and megakaryocytic markers ( glycoprotein IIb , platelet factor 4 ) as well as Spi-1 and Fli-1 transcripts at permissive temperature .
despite this drastic effect in vivo , the in vitro immortalization of erythropoietin-dependent erythroid progenitors unexpectedly occurred at low frequency , and all four cell lines established expressed both erythrocytic ( globins ) and megakaryocytic markers ( glycoprotein iib , platelet factor 4 ) as well as spi-1 and fli-1 transcripts at permissive temperature .
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