Single-cell reverse transcriptase -polymerase chain reaction ( RT-PCR ) analysis was applied to this culture system to investigate gene expression of diverse receptors , markers of differentiation , and transcription factors ( EKLF , GATA-1 , GATA-2 , p45 NF-E2 , PU . 1 , and SCL/Tal1 ) at discrete stages of erythropoietic development .
single-cell reverse transcriptase -polymerase chain reaction ( rt-pcr ) analysis was applied to this culture system to investigate gene expression of diverse receptors , markers of differentiation , and transcription factors ( eklf , gata-1 , gata-2 , p45 nf-e2 , pu . 1 , and scl/tal1 ) at discrete stages of erythropoietic development .
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