Transfection of trans-dominant negative expression vectors of ras and raf , together with AP-1 -dependent reporter constructs , as well as Western blot analysis using anti-ERK ( extracellular signal-regulated kinase ) antibodies , indicated that the Ras/Raf/ERK pathway did not appear to mediate the effect of the antioxidant .
transfection of trans-dominant negative expression vectors of ras and raf , together with ap-1 -dependent reporter constructs , as well as western blot analysis using anti-erk ( extracellular signal-regulated kinase ) antibodies , indicated that the ras/raf/erk pathway did not appear to mediate the effect of the antioxidant .
O O B-DNA_molecule I-DNA_molecule I-DNA_molecule I-DNA_molecule O B-DNA_domain_or_region O B-DNA_domain_or_region O O O B-protein_molecule O O O O O O O B-other_name I-other_name I-other_name O B-protein_family_or_group I-protein_family_or_group B-protein_molecule I-protein_molecule I-protein_molecule O O O O O O O O O O O O O O O O O O O