Subsequent studies have revealed that ( i ) cytoplasmic RelA is stably associated not only with I kappa B alpha but also with other ankyrin motif-rich proteins including the products of the NF-kappa B2 ( p100 ) and NF-kappa B1 ( p105 ) genes ; ( ii ) in contrast to RelA -I kappa B alpha , RelA -p100 cytoplasmic complexes are not dissociated following tumor necrosis factor alpha activation ; ( iii ) p100 functions as a potent inhibitor of RelA -mediated transcription in vivo ; ( iv ) the interaction of RelA and p100 involves the conserved Rel homology domain of both proteins but not the nuclear localization signal of RelA , which is required for I kappa B alpha binding ; ( v ) p100 inhibition of RelA function requires the C-terminal ankyrin motif domain , which mediates cytoplasmic retention of RelA ; and ( vi ) as observed with I kappa B alpha , nuclear RelA stimulates p100 mRNA and protein expression .
subsequent studies have revealed that ( i ) cytoplasmic rela is stably associated not only with i kappa b alpha but also with other ankyrin motif-rich proteins including the products of the nf-kappa b2 ( p100 ) and nf-kappa b1 ( p105 ) genes ; ( ii ) in contrast to rela -i kappa b alpha , rela -p100 cytoplasmic complexes are not dissociated following tumor necrosis factor alpha activation ; ( iii ) p100 functions as a potent inhibitor of rela -mediated transcription in vivo ; ( iv ) the interaction of rela and p100 involves the conserved rel homology domain of both proteins but not the nuclear localization signal of rela , which is required for i kappa b alpha binding ; ( v ) p100 inhibition of rela function requires the c-terminal ankyrin motif domain , which mediates cytoplasmic retention of rela ; and ( vi ) as observed with i kappa b alpha , nuclear rela stimulates p100 mrna and protein expression .
O O O O O O O O B-protein_subunit B-protein_subunit O O O O O O B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule O O O O B-protein_family_or_group I-protein_family_or_group I-protein_family_or_group O O O O O O O O O O O O O O O O O O O O O O O O B-protein_subunit B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule O B-protein_subunit O O O O O O O B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule O O O O O B-protein_molecule O O O O O O B-protein_subunit O O O O O O O O O O O B-protein_subunit O B-protein_molecule O O B-protein_domain_or_region I-protein_domain_or_region I-protein_domain_or_region I-protein_domain_or_region O O O O O O B-protein_domain_or_region I-protein_domain_or_region I-protein_domain_or_region O B-protein_subunit O O O O O B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule O O O O O B-protein_molecule O O B-protein_subunit O O O B-protein_domain_or_region I-protein_domain_or_region I-protein_domain_or_region I-protein_domain_or_region O O O O O O B-protein_subunit O O O O O O O O B-protein_molecule I-protein_molecule I-protein_molecule I-protein_molecule O O B-protein_subunit O B-protein_molecule O O B-other_name I-other_name O