The ORF was expressed in bacteria , and the 30-kD protein product was shown to bind DNA in a sequence-specific manner by mobility-shift assays , Southwestern blot analysis , and methylation interference .
the orf was expressed in bacteria , and the 30-kd protein product was shown to bind dna in a sequence-specific manner by mobility-shift assays , southwestern blot analysis , and methylation interference .
O B-DNA_domain_or_region O O O B-mono_cell O O O B-protein_molecule I-protein_molecule I-protein_molecule O O O O B-DNA_N/A O O B-other_name I-other_name O B-other_name I-other_name O B-other_name I-other_name I-other_name O O B-other_name I-other_name O