Ritual sacrifice
Ritual sacrifice was the practice of killing a lifeform to serve as a religious offering.

Sacrifice of animals
In Klingon culture, the traditional method for making Var'Hama candles from targ shoulders required three of the animals from the Hamar Mountains to be ritually sacrificed at dawn. ()

A traditional Klingon wedding ceremony involved the ritual sacrifice of a targ before the wedding feast. ()

Sacrifice of sentient beings
The D'Arsay culture included ritual sacrifice. In their mythology, Ihat constantly sought to escape being put on Masaka's sacrificial slab. ()

On ancient Qo'noS, live sacrifices were offered to the tyrant Molor at his shrines, built at the seven entrances to the planet's underground volcano system. ()

The harvesting of Kelpiens by the Ba'ul in the mid-23rd century was codified in Kelpien culture as a ritual sacrifice and overseen by priests. ()

In 2267, when "Lord" Garth of Izar wondered if the unenthusiastic "heir apparent" James T. Kirk might want a larger role in his coronation ceremony, he suggested Human sacrifice instead. After Kirk graciously declined ("No, I wouldn't enjoy that at all."), saying Garth needed him alive, the insane man agreed, saying he had other candidates. ()

On Kelis' homeworld, the inhabitants once sacrificed a person every year in honor of winter. At some point, for forgotten reasons, the sacrifice was replaced with a , and the  in which the sacrifice took place was converted into a theater. ()

Child sacrifice 
Child sacrifice was the practice of killing a child and offering it to a god.

In 2376, attempting to dissuade B'Elanna Torres' plan to voluntarily return to the Barge of the Dead to see her mother again, Captain Kathryn Janeway explained that there was a limit to freedom of worship aboard , giving the hypothetical example of Torres wishing to sacrifice a child to the gods as a comparison. Torres found the analogy to be absurd. ()

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