2.6. Blood Biochemical Analysis
Fasting blood samples (5 mL) were collected, preoperatively, in vacuum tubes (Vacuette do Brasil Ltd.a, Campinas, Brazil) containing EDTA anticoagulant (Merck KGaA, Darmstadt, Germany) via cubital venipuncture for plasma preparation. Circulating levels of total cholesterol, HDL, LDL, triglycerides, and glucose were assessed using commercial kits (Gold Análise Diagnostica Ltd., MG, Brazil) for the SpectraMax i3 analysis system (Molecular Devices, Sunnyvale, CA, USA). The inflammatory markers leptin and adiponectin were assessed using commercial ELISA kits (R&D Systems, Minneapolis, MN, USA, and BioLegend, Sellex Inc., Washington, DC, USA), following manufacturer recommendations.