Sample size calculation and randomization
Using the G-power version 3.1.9 computer program, the sample size was calculated. The study’s power had been set at 80%, and the alpha level was 0.05. The estimated minimum sample size(n) was 15 patients for each group, then it was increased to 20 patients per group. Block randomization was done by an independent researcher to assign sample numbers equally to each group using a1:1:1 allocation ratio and a six-block size by a randomization software (Sealed Envelope Ltd.2021 (