tumor
TUMOR
Using the CanPatrol CTC‐enrichment method, CTCs were found in blood samples from 227 patients. Gene expression in mesenchymal and epithelial cells is indicated by red and green fluorescent signals, respectively. A leukocyte marker called CD45 is expressed and is shown by a white fluorescence signal. Thus, three subpopulations were identified: (1) epithelial circulating tumor cell (E‐CTC), (2) epithelial/mesenchymal hybrid circulating tumor cell (E/M‐CTC), and (3) mesenchymal circulating tumor cell (M‐CTC) (Figure Status of circulating tumor cells (CTCs) undergoing epithelial‐mesenchymal transition before surgical resection in patients with and without EHR. (A) CTC subpopulations and their relationship with survival. CTCs were stained for epithelial markers (EpCAM and CK8/18/19, red fluorescence) and mesenchymal markers (Vimentin and Twist, green fluorescence) and classified as epithelial CTCs (E‐CTCs), epithelial/mesenchymal hybrid CTCs (E/M‐CTCs), and mesenchymal CTCs (M‐CTCs). (B)The total CTC count and the percentage of the CTC in the 227 patients. (C) The total CTC positive rate of the 227patiets and the subgroups of patients with and without EHR. Comparison of total CTC counts (D), E‐CTC counts (E), E/M CTC counts (F), and M‐CTC counts (G) in patients with and without EHR.