2.5. Identification and Quantifications of Serum Proteins by Mass Spectrometry
The identification and quantifications of serum proteins from our cohort were performed exactly as previously described [The mass spectrometry data were obtained using the All-Encompassing Data Collection (DIA) mode with Version of Thermo Xcalibur software 4.3.73.11. Internal calibration was conducted utilizing the lock mass function with the siloxane ion with a mass-to-charge ratio of 445.12003. The spray voltage was adjusted to 2100 V, while maintaining a constant heated capillary temperature of 275 °C. Full scan mass spectra were recorded in the Orbitrap, ranging from 350 to 1000 The initial step involved converting the raw data files into mzML format using MSConvert version 3.0.21188. Signal extraction and quantification were then conducted through DIA-NN version 1.8 [