Running the CyPath Lung assay pipeline
cancer, anomalies
CANCER, LUNG
Having developed the two stages of the CyPath Lung assay, we could now assemble the full pipeline, including quality control steps, determination of predictive variable values, and classification of samples (Fig. CyPath Lung data processing pipeline. The schematic represents the following main elements: QC measures (top two diamonds), retrieval of the data and running the model (two squares in the middle) and final determination whether the sample is likely to be cancer or not (bottom diamond)Sample quality assessment begins by ensuring that the data file for each collection tube is readable and that its encoded data matrix is complete. Next, the Time signature is used to examine fluorescence channels in each tube and to remove anomalies in the flow rate arising from bubbles or clogs during sample acquisition [The next step in the assay pipeline (Fig. The final step of the pipeline (Fig. CyPath Lung performance on LSRII samples. 