Embryo morphology evaluation
Embryo morphology was assessed: (i) 16 to 18 hours post-ICSI ± 1hour, (ii)
44 hours ± 1hour post-ICSI, for day two evaluation (iii) 68 hours
± 1hour post-ICSI, for day three evaluation (iv) 116 hours ± 2hour
post-ICSI for day five evaluation and (v) 120 hour ± 2hour post-ICSI for
late day five evaluation (transfer time). Embryo morphology was performed using
an inverted Nikon Diaphot microscope (Eclipse TE 300; Nikon, Tokyo, Japan) with
a Hoffmann modulation contrast system under 400X magnification and was considere
to choose best embryos for transfer.