2.8. Inflammatory Markers
tumor necrosis
TUMOR NECROSIS
All participants underwent overnight fasting, and blood samples were collected at baseline and endpoint. Enzyme-linked immunosorbent assay (ELISA) was utilized to analyze the levels of inflammatory markers, including interleukin 6, interleukin 10, and tumor necrosis factor-α. The Milliplex Map Human Cytokine/Chemokine Magnetic Bead kit (HCYTOMAG-code 60K, Millipore, Darmstadt, Germany) was employed for the accurate measurement of these markers in the blood samples.