4.7. Immunohistochemical Analyses Applied to TLR4, TRIF, TBK1, IRF3, IFN-γ, CX3CR1, FOXP3, PD-L1, CTLA4, and iNOS in Bladder Biopsies, Both before and after OncoTherad
Both pre- and post-treatment bladder biopsy samples (Subsequently, the immunolocalization of antigens such as TLR4, TRIF, TBK1, IRF3, IFN-γ, CX3CR1, FOXP3, PD-L1, CTLA4 and iNOS was performed using specific primary antibodies (Ten (10) fields (at 400× magnification) were selected for each patient and antibody to assess antigen immunoreactivity intensity in bladder tissue. Immunostaining results were analyzed in Image J software version 1.54f. Macro profile analysis was used to quantify positive cells [