Analysis of the intraoral photographs using the DenTiUS Deep Plaque software
tooth
PLAQUE, DENTAL PLAQUE, PLAQUE
The digital quantification of the bacterial plaque in the photographic images was carried out using an image processing program of our design named DenTiUS Deep Plaque [DenTiUS Deep Plaque is an application based on digitally processing photographic images of dentition to quantify the bacterial plaque on tooth surfaces. Its use is indicated for analysing dental plaque evolution patterns and evaluating the effectiveness of different oral hygiene measures. The software allows the automatic determination of the levels of visible, non-visible, and total dental plaque (Fig. 
Determination of dental plaque levels by DenTiUS Deep Plaque software: graphical representation of plaque-free teeth, visible plaque, and non-visible plaqueA series of clinical indices can be calculated for each type of plaque, and the following are those that were evaluated in the present trial:
Plaque area: Percentage of the tooth surface with dental plaque.Plaque growth area: The growth rate of the dental plaque area per unit of time in hours (percentage area/hour).These indices were calculated absolutely on the analysed image without using a previous reference and relatively on the analysed image utilising the moment of professional dental cleaning as a reference (Figs. 
Quantification of visible plaque and non-visible plaque levels on day 4 (left) and day 8 (right) in an example patient in test group A
Quantification of visible plaque and non-visible plaque levels on day 4 (left) and day 8 (right) in an example patient in test group B