Soluble immunomodulatory factors
Plasma was prepared by centrifugation of the remaining EDTA blood and stored in aliquots at -80 °C. In a pilot experiment, EDTA plasma of eight non-vaccinated patients before treatment initiation with AI was investigated. Therefore, an Olink® assay was performed which used matched pairs of oligonucleotide-labelled antibodies that were subsequently quantified by standard real-time PCR (Olink AB, Uppsala, Sweden). This enabled the simultaneous analysis of 92 different immunomodulatory parameters (for a detailed list of all factors see Supplementary Figure S