Statistical analysis
REGRESSION, ADHESION, PLAQUE
Statistical analyses were performed using IBM SPSS Statistics Ver 23.0 (IBM, Tokyo, Japan). Corresponding t-tests were used to compare the number of bacteria in saliva at the time of plaque adhesion and at the time of plaque removal. Statistical analyses of bacterial counts before brushing, after brushing, and after wiping or rinsing were performed using the paired-samples t-test, followed by multiple comparisons using the Holm–Bonferroni method. Patient factors affecting the Total Bacterial count before brushing were examined using the Mann–Whitney U test, Spearman's rank correlation coefficient, and multiple regression analysis.