PRP platelet flow cytometry
EVENTS
Immunophenotyping of platelets in PRP was carried out in a NovoCyte Quanteon™ flow cytometer (Agilent Technologies). The samples were diluted tenfold with phosphate-buffered saline (PBS) and stained with CD61 (557291 BD Biosciences), CD63 (353011 BioLegend) and P-selectin (304910 BioLegend) at ambient temperature for 15 min. The gating strategy was aimed at counting proportions of CD63 and P-selectin positive events in CD61 + pools.