2.7. Dot Blot Protocol for CCK and Ghrelin Analyses
Serum CCK and ghrelin levels were measured via Dot-blot. Briefly, five microliters of serum samples was spotted on a nitrocellulose membrane to then be processed via Western blotting. CCK and ghrelin levels were immunodetected using anti-ghrelin (Santa Cruz, CA, USA, dilution 1:250) and anti-cholecystokinin (Santa Cruz, CA, USA, dilution 1:250) anti-bodies.