2.4. Measurement of Hepatic Fat Content and Composition
BEST
On day 15, at 7:15 a.m. after an overnight fast, the hepatic lipid content and lipid composition (the fraction of hepatic SFA, MUFA, and PUFA) were determined by 1H-MRS on a 3T MR system (Achieva 3T-X Philips Healthcare, Best, The Netherlands) using a STEAM sequence (repetition time (TR) of 4500 ms/echo time (TE) of 20 ms/mixing time (TM) of 16 ms, spectral bandwidth of 2000 Hz, and 2048 data points) and fat content and fat composition was determined as described earlier [