Introduction
CORONAVIRUS, CORONAVIRUS DISEASE 2019, SEVERE ACUTE RESPIRATORY SYNDROME
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a global health crisis that is driving efforts to identify alternative medicinal plants to be used in COVID-19 treatment. There is evidence that the Acanthaceae family member Liquid chromatography-tandem mass spectrometry (LC–MS/MS) is a powerful technique widely used in biological analysis, including in the screening of lead candidates, determination of metabolites, and pharmacokinetic investigationTaken together, the previous method was applicable for determining the four major diterpenoids (AP1, AP3, AP4, and AP6) in only plasma samplesThe present study is the first investigation of oral dosing of a high dose administration of 