4.1. Chemicals and Reagents
All chemicals (analytical grade) used in the experiments, such as sodium carbonate, potassium persulfate and salicylic acid were purchased from Aladdin-Reagent Co., Ltd. (Beijing, China) and Sinopharm Chemical Reagent Co., Ltd., Shanghai (China), and deionized water was used. The in vitro scavenging capacity against MDA, SOD, GSH and T-AOC were determined using commercial kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).All the liquid high-fat loaded diet food-grade materials, casein, sucrose, lactose, maltodextrin and monoglyceride, were purchased from Henan Wanbang Industrial Co. Ltd. Butter for animal fat was purchased from Kaptein B.V. Rotterdam, Netherlands. Purified water was purchased from a Nanjing local supermarket.