2.4. Microbiome Analysis
16Sv4 amplicons generated from fecal samples collected in OMNIgene-Gut kits (DNA Genotek) were sequenced on a MiSeq platform (Illumina, San Diego, CA, USA) at Microbiome Insights (Vancouver, BC, Canada). MiSeq-generated Fastq files were quality-filtered and clustered into 97% similarity operational taxonomic units (OTUs) using the mothur software package and the Greengenes v13.8 database [