4. Discussion
DOMS, muscular contractions, Muscle fatigue, fatigue
In this study, NPN_1 supplementation improved strength recovery, reduced fatigue and suppressed myostatin expression in a healthy male population following EIMD. Previously, we have shown that NPN_1 induced an increase in phosphorylated S6 with a concomitant decrease in atrophy-associated genes in vitro [Exploratory iAUC analysis showed a 54% improvement in performance of isokinetic leg extension with NPN_1 supplementation compared to placebo over the 72 h after EIMD. NPN_1 supplementation significantly increased muscle strength recovery (Muscle fatigue is a major symptom of DOMS. The fatigue index measurements at 48 and 72 h post-EIMD were calculated using the highest and lowest force recorded during these sets [Myokines are cytokines released by muscle cells in response to muscular contractions [As predicted using AI, the in vitro effects observed for NPN_1 [A limitation of the current study would be the comparison of NPN_1 to SMCC rather than an unhydrolysed 