Measurement of serum inflammatory cytokines by ELSIA assay
STERILE
Venous blood of the CG and EG was collected in a sterile non-anticoagulant test tube before and after treatment. The immune transmission turbidimetry was used according to the procedure of CRP kit, and automatic biochemical analyzer was used to detect the level of CRP. The levels of serum cytokines were measured by ELISA (Elabscience) according to the manufacturer’s instructions.