2.5. Outcomes
fasting glucose, GDM, weight gain
PRE-ECLAMPSIA, GESTATIONAL HYPERTENSION, GDM, VITAMIN D DEFICIENCY, DIABETES AND PREGNANCY
Primary outcomes included maternal serum 25OHD and lipid levels. Serum 25OHD levels were assessed using electrochemiluminescence technology for immunoassay (Roche Cobas E411, Switzerland) and classified as sufficient (25OHD ≥ 50 nmol/L), insufficient (25OHD 25 to < 50 nmol/L), and deficient (25OHD < 25 nmol/L). Currently, there are no standardised criteria for 25OHD levels. The UK Scientific Advisory Committee on Nutrition has proposed that a serum 25OHD level < 25 nmol/L indicates a concentration at which the risk of poor musculoskeletal health is increased at a population level and is therefore considered indicative of vitamin D deficiency [Secondary maternal outcomes included fasting glucose, 1 h post-load glucose, 2 h post-load glucose, GDM, gestational hypertension, pre-eclampsia, caesarean section, and total gestational weight gain. Plasma glucose levels were assessed by the Abbott Alinity c glucose enzymatic (Hexokinase) assay, Germany. GDM was diagnosed based on the International Association of Diabetes and Pregnancy Study Groups criteria [