4.6. Cell Production and Preparation
MYCOPLASMA
Three weeks prior to transplantation, subcutaneous abdominal adipose tissues were obtained using liposuction. This procedure was executed by a skilled plastic surgeon in the operating room, using local anesthesia. The liposuction yielded an average volume of 176 mL, with a range from 140 mL to 250 mL. Following the procedure, patients were observed for 4 h before discharge. The harvested liposuction samples were transported under controlled conditions to the GMP facility of CHA Biotech Co., Ltd. (Pangyo, Seongnam, Republic of Korea). The lipoaspirate underwent aseptic processing using established methodologies [After 12 h, the adhered cells were subjected to a 12-h serum starvation phase, followed by cultivation in DMEM supplemented with 10% FBS and 10 ng/mL recombinant matrilin-3 (Aviva Systems Biology, San Diego, CA, USA) for 5 days. The medium, including matrilin-3, was changed daily. The pretreated cells were subsequently cultured at a density of 3.0 × 10The formed ASC spheroids were gathered, vial-packaged, and meticulously examined for the absence of pathogens like viruses, bacteria, mycoplasma, and endotoxins. For transplantation, autologous ASC spheroids primed with matrilin-3 were transported to the operating room, standardized at 1.0 × 10