Methods
coronary artery disease
BLOOD, CORONARY ARTERY DISEASE
This was a prospective, randomized study of patients undergoing elective coronary angiography on suspicion of coronary artery disease from October 2016 to September 2017. The methods have been published previously (Árnadóttir et al., Blood samples were drawn every 15 min for the first 3 h, followed by every 30 min for the next 3 h from a peripheral venous catheter in the cubital vein and collected in serum or plasma tubes. For this study EDTA plasma was used. Samples were centrifuged for 10 min at 3,000 g aliquoted and stored at − 80 °C until analysis. There were no additional freeze-thaw cycles prior to analysis.