Generation of monocyte-derived DC (mDCs) and co-culture experiments
HIV Gag)
As a T cell functional analysis method established by our group, the S1-loaded mDCs were co-cultured with the autologous memory T cell subsets freshly purified from the same individual. In this co-culture method, the specificity of T cell responses against S1 protein was previously confirmed by comparing the results obtained with mDCs loaded with an unrelated protein antigen (HIV Gag) and with mDCs which were not loaded with a specific proteinPrior to the establishment of the co-cultures, the memory T cell subsets were purified from the peripheral blood samples freshly obtained from the volunteers enrolled to the study. CD19