Procedures
prostate
PROSTATE
Leukapheresis was performed 14 days prior to CryoIT followed by monocyte enrichment using the ELUTRATM System (Supplementary S2). Autologous iDCs were produced according to protocol (Supplementary S2).Cryoablation of the prostate was performed under general anesthesia and ultrasound guidance as a cyclic freeze–thaw process (Supplementary S3). Directly prior to the first freezing cycle, eight core prostate biopsies were sampled transrectally. Succeeding cryoablation, the pre-specified number of viable iDCs was injected intratumorally into the prostate. All patients received cyclophosphamide, 300 mg/m2 intravenously three days prior to CryoIT, and low oral doses (50–100 mg/day) metronomically every other week from week 2 to week 26 post-treatment to avoid Treg increases that might diminish incipient immune responses (Supplementary S5, Fig. 