Author contributions
ZXY, WLC, TH, HW, and JC concepted and designed the study.ZXY, DL, WLC, QYQ and JC analyzed and interpreted the data. WLC, DL, TH and JC prepared cfDNA and tissue samples. ZXY, WLC, TH, XZL, CHL, XXL, HW and JC conducted ultradeep NGS assays and bioinformatic analysis. ZXY, WG, AF, TH, HW, XZL, CHL and JC drafted and revised the manuscript. ZXY, CHL, XZL, and DL conducted statistical analysis. JC, ZXY and HW provided administrative, technical or material support. All authors read and approved the final manuscript.