Tumor mutational burden
tumor
MINOR, TUMOR, SOMATIC MUTATIONS
TMB was defined as the number of non-synonymous somatic mutations (SNVs and small indels) per mega-base in coding regions (mut/Mb). Coding variants (except for intronic splicing ones, therefore exons-only which represent 1.59 Mb), without synonymous nor polymorphisms (> 0.1% minor allele frequency [MAF]) and recurrent variants covered enough (not tagged as Low_Depth) were considered in all those calculations. Because the median and range of mutational load have been shown to vary across tumor types, we focused on the top 10% patients with the highest TMB scores and determined the log-rank p value for difference in survival among the 10% top TMB and 90% bottom TMB subgroups of patients.