Study design and participants
infection, musculoskeletal injury, asthma
DISEASE, VASCULAR PROBLEM, INFECTION, ASTHMA, HYPERTENSION, DISEASES
The trial (IR.RAZI.REC.1400.001) was approved by the Ethics Committee of the Kermanshah Razi University and registered in the Iranian Clinical Trial Registration Center under the code IRCT20210414050965N1; written informed consent was obtained from all participants, including agreement of the patients to participate as volunteers and feasibility to leave the study.This study is quasi-experimental with pre-test and posttest design and placebo groups. The statistical population of this study was non-athlete men aged 20 to 30 years in Kermanshah. To select the subjects, invitations for participating in the clinical trials were posted on Telegram, Instagram, and WhatsApp. Forty-eight individuals volunteered, among which 26 individuals who met the inclusion criteria were selected as subjects. Inclusion criteria include; Vit D deficiency (25 levels of hydroxyvitamin D below 50 nmol/L), lack of underlying disease, musculoskeletal injury, regular physical activity in the last 6 months, infection with COVID-19, any kind of supplementation, and smoking. One day before the start of the study, the subjects were given the necessary explanations about the research conduction and signed the informed consent of participation in the research. Also, the three-day estimated food record, nutrition (including dietary history, food frequency, eating habits, and behaviours), and health questionnaires were filled. Health questionnaires include; history of previous diseases (hypertension, asthma, vascular problem), family history of diseases, medical history, assessment of physical activity level (activity level, duration of activity), and anthropometric characteristics (weight, height, waist). Subjects were then randomly divided into two groups: Experimental (Exp; n = 13) and Control (C; n = 13); the lottery was used to assign the subjects in the groups. It should be noted that one individual in each group refused to continue the study protocol. Consort flow diagram for the study