Metabolites
Glucose and insulin were measured in plasma on the Cobas 6000 instrument (Roche Diagnostics, USA) using an enzymatic hexokinase assay or electrochemiluminescence, respectively. HbA1c was determined using the HPLC D10 instrument (Bio-Rad, USA). High sensitivity C-reactive protein (hsCRP) was measured in plasma with the immunoturbidometric method assay (Abbott Architect, USA). Fructosamine was measured in serum LDL = (1.06*Chol) – (1.03*HDLC) – (0.117*Trig) – (0.00047*(TRIG*(Chol-HDLC))) + (0.000062*(Trig*Trig)) – 9.44