2.10. Bioinformatic Processing and Statistics
bleeding
REGRESSION, BLEEDING, PLAQUE
Clinical data and plaque and bleeding index were compared between groups and sampling times by multiple linear regression and adjusted for baseline levels (ANCOVA) in R (version 4.2.3) using R studio (IDE version 2023.04.1 + 446).The supragingival microbiota was characterized and compared by relative abundance and visualized by principal component analysis (PCA), while statistically significant differences in microbial community compositions were tested by linear discriminant analysis effect size (LEfSe). Data on relative abundance were corrected for multiple dependent associations using Benjamini—Hochberg correction [Differences in cytokines, albumin, and enzyme activity between groups were analyzed by ANCOVA on log10-transformed data adjusted for baseline levels, using R (version 4.2.2) and R studio (IDE version 2023.06.2 Build 561). A 