Microbiological analysis
non-lactose
FRANKLIN, BRAIN, STERILE, CAVITY, STERILE, HEART, CONJUNCTIVA
Swabs for microbiological evaluation were collected from the conjunctiva of the defective socket in both groups before the insertion of the prosthesis and again three months later, following the removal of the artificial eye using a suction cup. Prior to sample collection, patients were instructed not to use antibiotics, either topically or systemically, for at least five days before the sample collection and to retain from washing the area with any antiseptic solution other than saline.Sterile rayon swabs (Rayswab, Difco) were soaked in 9% sodium chloride from the inferior fornix of the anophthalmic cavity. Each swab was then cut off and placed in a tube (Becton, Dickinson & Company, Franklin Lakes, NJ, USA) containing 4 mL of sterile Brain Heart Infusion Broth (BHI). The tube was sealed, and cultures were promptly transported to the laboratory.Isolates were identified through microscopy, culture methods, and biochemical tests. The samples underwent the procedures of conventional microbiology diagnostic method [After 48 h incubation the colonies appeared will be subjected to biochemical tests to be identified. For isolates grew on blood agar only gram film from colonies was done and gram positive cocci was further identified using catalase test, bile esculine test, side and tube coagulase test.For isolates grew on blood agar and macck agar gram stained film was done to confirm that it is a gram negative bacilli and the biochemical tests were done (TSI triple sugar iron agar, urease test, citrate test, and MIO-test) for non-lactose fermenting colonies oxidase test was added.Gram stain film from colonies appeared on SDA was done. After the prescribed incubation period, the identification of the isolate was carried out through Gram staining, microscopy, and biochemical reactions.