2.8. Caco-2 Cells Viability
The viability of Caco-2 cells was determined using the colorimetric MTS assay. Caco-2 cells were seeded in triplicate into 96-well microtiter plates at a density of 8000 cells/well. After being cultured in the presence of high-glucose DMEM supplemented with 1% L-glutamine and sodium pyruvate, 10% fetal bovine serum, 1% L-alanyl-L-glutamine, and 1% non-essential amino acids for 72 h, the cells were treated with LPS (0, 30, 60, and 120 pg/mL) or with a polyphenol extract (0, 25, 50, and 100 µg/mL).The plates were incubated at 37 °C in a humidified, 5% COAfter 48 h of treatment, the conditioned medium was removed and 20 μL MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium) was added to 100 μL of fresh culture medium and added to each well and incubated for 3 h at 37 °C in a humidified, 5% CO