Masson’s Trichrome (MT) staining
Connective and fibrotic tissues are stained blue, nuclei are stained deep brown to black, and the cytoplasm is stained red with MT staining. LV tissue was collected from HF animals with and without DS treatment and fixed in 10% neutral buffered formalin. The samples were sectioned and stained at the Translational Pathology Shared Resource at Vanderbilt University.