Immunohistochemistry
tumor
TUMOR
IHC for Ki-67, CD3 and CD8 was performed by Mosaic Laboratories and quantification derived from an annotation, including all tumor and intervening stroma in the tumor nest. CD3 (mouse clone LN10) immunoglobulin (Ig)G1 antibody (catalog no. NCL-L-CD3-565) was purchased from Leica Biosystems. The CD8 (mouse clone C8/144B) IgG1κ antibody (catalog no. M7103) and the Ki-67 (mouse clone MIB-1) IgG1κ antibody (catalog no. M7240) were purchased from Dako. Antibodies were diluted per the manufacturer’s instructions. All antibodies were stored at 2–8 °C.