5. Limitations
Our conditioning paradigm introduced a colour clue (coloured meal during and after conditioning versus non-coloured meal pre-conditioning), but we do not know whether conditioned postprandial dissatisfaction was selective to the colour or if it would also affect the non-coloured meal; indeed, other forms of conditioning express generalisation and affect related stimuli [For this pilot study, a small sample size was included due to the complexity and invasiveness of the study; an interim sample size calculation justified no further inclusion for practical and ethical considerations, but once the concept is proven, a larger study with a less invasive methodology is indicated. Furthermore, only women were included and the effect of conditioning on men remains to be explored.This pilot study, proving a new concept, opens a series of questions, particularly in relation to the specificity versus generalisation of the conditioned response, extinction interval and the relation between aversive stimulus/conditioned response [