VEGF quantification
AMI
Supernatants from AMI patients and FHD ProtheraCytes were collected after 9 days of expansion in StemFeed cell culture medium (Eurobio, France) and stored at −80 °C until analysis. Supernatants were thawed and VEGF levels were measured using the Human VEGF QuantiGlo ELISA Kit (R&D Systems, MN, USA) according to the manufacturer’s instructions with the SpectraMax L (Molecular Devices, San Jose, CA USA). The Immunoassay Control Set 732 for Human VEGF (R&D Systems) was used as a positive control and StemFeed medium as a negative control.