Blood sampling
BLOOD
Blood samples were collected from antecubital veins of subjects according to the guidelines of the Clinical and Laboratory Standards Institute to identify changes in serum markers immediately before and after music therapy. Blood samples were transferred to SST tubes and immediately centrifuged at 3000 rpm for 10 minutes at 4 °C. The serum was subsequently removed and stored in 1 ml aliquots at − 80 °C until analysis (Scheme 