No GLUT4 translocation or mTORC2 activation upon clenbuterol treatment
SECONDARY
To gain further insights into the putative underlying molecular mechanisms through which clenbuterol stimulates peripheral glucose uptake, skeletal muscle biopsies were taken in the overnight fasted state after 14 days of clenbuterol treatment and were examined for GLUT4 translocation, mTORC2 activation, and markers of mitochondrial capacity. The secondary outcome of the study was defined as the fraction of total GLUT4 detected at the cellular membrane, reflecting GLUT4 translocation. Both GLUT4 translocation and total GLUT4 content remained unaffected by clenbuterol treatment (