Materials and methods
The study protocol was following the Declaration of Helsinki (revised in October 2018) and was approved by the Research Ethics Committee of the faculty of Dentistry, Ain Shams University (FDASU-RecD061808). A total number of thirty patients undergoing periodontal therapy were enrolled in this prospective cohort randomized clinical and biochemical trial consecutively from the outpatient clinic of Periodontology Department, Faculty of Dentistry, Ain Shams University, Cairo-Egypt between January 2020 to November 2021. Participants were informed verbally and written informed consent was obtained.