Radiation-induced vaginal mucosal injury was associated with vaginal microbiota CST-C
vaginal injury
All DNA samples from vaginal swabs were sequenced by 16S-rDNA in order to explore the impact of radiotherapy on vaginal microbiota and the therapeutic effects of The effects of radiotherapy and Changes in OTUs before and after treatment were analyzed in each group by Venn diagram (Fig. The linear discriminant analysis effect size (LefSe) was applied to further evaluate vaginal microbiota abundance differences between assigned taxaDifferences in bacteria and function between patient groups. (In view of the large differences in total amount of bacteria from vaginal swabs of different patients, relative abundance changes may not fully reflect the impact of treatment on the absolute amount of The vaginal microbiota CST of all patients was analyzed to evaluate the relationship between vaginal microbiota and clinical indices. In the pre-treatment control group, the incidence of CST-A, -B, -C and -D was 7.7% (2/26), 30.8% (8/26), 53.8% (14/26), and 7.7% (2/26), respectively. After radiotherapy, all patients showed varying degrees of vaginal injury. Six patients (dominated by Relationship between vaginal microbiota composition and clinical indexes. (To further investigate the correlation between radiation dose, vaginal microbiota and radiation injury degree, redundancy analysis (RDA) was performed on samples from control patients after radiotherapy (Fig. 