Cholesterol efflux ability
Cholesterol efflux ability of participant plasma from primary macrophage was measured using a commercially available kit (Abcam, ab196985) following the manufacturer’s instructions with the following modifications: primary macrophages were lipid loaded with one-half of the recommended labeled cholesterol for 4 h prior to incubation with either participant plasma in equilibration buffer to a final concentration of 2% or FBS in equilibration buffer to a final concentration of 2%. Cholesterol efflux from macrophage was measured after 2 h of incubation with participant plasma.