Blood biomarker measurement
BLOOD
Patient serum was drawn on day 1, 4 and 7 to measure angiotensin II, cortisone, cortisol and aldosterone levels. Patient citrated plasma was used to measure VWF and D-Dimer. All assays were performed according to the manufacturer’s instructions. Concentrations were calculated against standard curves and used for statistical analysis. Blood glucose was measured by glucometer (Glucocare Sense, RMD Mediaids ltd, Haryana, India) and sodium, urea and potassium were measured as part of routine clinical care.The circulating blood biomarkers were assayed using the following kits: Angiotensin II Enzyme-linked Immunosorbent Assay (ELISA) (E13652287, Sincere Biotech Co.,Ltd, New Taipei City 221, Taiwan (R.O.C.)); Cortisone ELISA (E13652408, Sincere Biotech Co.,Ltd, Sincere Biotech Co.,Ltd, New Taipei City 221, Taiwan (R.O.C.)); Architect Cortisol chemiluminescent microparticle immunoassay (CMIA) (8D15-25, Abbott Laboratories, Abbott Park, Illinois, USA); Liaison Aldosterone chemiluminescent immunoassay (CLIA) (310,450, DiaSorin, Sallugia, Italy); Innovance VWF:Ac (10,487,040/OPHL03, Siemens Healthcare Diagnostics, Marburg, Germany); and Tina-quant D-Dimer Gen.2 particle-enhanced immunoturbidimetric assay (04,912,551, Roche Diagnostics GmbH, Mannheim, Germany).