4.3. Plant Materials and Extracts Preparation
Imperial Chrysanthemum is produced in Zibo, Shandong Province of China followed by 40 °C low temperature drying of the products. The dry sample (2020-c-02) was collected during December 2020 and identified by Engineering Technology Research Center of Characteristic Biological Resources. In this study the sample was ground to a powder and screened through a 120~200 mesh filter. The sample was stored at −80 °C before analysis.For the preparation of extracts, 100 g of the powdered sample were extracted with 12 L of deionized water at 100 °C for 90 min, followed by filtration through a 0.45 μm Whatman filter paper and evaporation under reduced pressure using a rotary evaporator. After concentration, the sample (IC-E) was dried by freeze dryer (ALPHA1-2/LD-Plus, Marin Christ, German) and stored at −80 °C. The TFC was determined according to “