Discussion
GDM, disorder of gut microbiota
OF PREGNANCY COMPLICATIONS, METABOLIC DISEASES, GDM
During pregnancy, the disorder of gut microbiota and abnormal glucose metabolism may be the possible mechanism of pregnancy complications such as GDM (The preliminary conclusion of this study is that GOS intervention has no significant effect on reducing the incidence of GDM and improving glucose and lipid metabolism. GOS a kind of prebiotics that can be selectively and selectively utilized by host microorganisms that confer a health benefit, while probiotics are defined as live microorganisms (Both the intervention group and the control group have similar relative abundances at the phylum level, including Several strengths and limitations should be taken into consideration. First, this study is a randomized controlled pilot trial. Subsequently, 16S rRNA gene was sequenced by Illumina Hiseq 2500 sequencing platform, a widely and reliable used high-throughput sequencing platform, which can ensure gut microbiota can be successfully identified. Secondly, the quality control in the process of sample collection can be guaranteed, which makes the sequencing quality high and accurate. However, some limitations should also be considered. The sample size of our pilot study is limited, and some confounding factors such as diet and exercise have caused some interference. Although it is difficult to control these confounding factors, we recorded these situations in the form of health education and questionnaire records. Moreover, this study was recruited in the same hospital, and the potential regional differences of microbiota cannot be evaluated. In general, our study provides an important basis for the intervention of prebiotic dietary supplements targeting gut microbiota in pregnancy on metabolic diseases of pregnancy. In the future, clinical trials with higher quality and larger sample size are needed to further verify the effect of prebiotic supplements.