2.2.2. Plasma Hormone Levels
BLOOD, EMD
Blood draws were taken every 12 h of each fast (0, 12, 24, and 36 h). Samples were centrifuged for 15 min within 10 min of collection and then stored at −80 °F. Ghrelin, GLP-1, PYY, PP, leptin, insulin, and GIP were quantified using a standard 96-well multiplex human metabolic hormone magnetic bead panel according to the manufacturer’s instructions (EMD Millipore Corporation, Billerica, MA, USA, Catalog # HMHEMAG-34K).