Detection of 
malaria
MALARIA
Samples for preparation of thick blood smears (TBS) and analysis by quantitative polymerase chain reaction (qPCR) were obtained within 24 hours before each immunization and at any time a participant was suspected of having symptomatic malaria. Only the TBS was used in real time for diagnosis of malaria (excluding participation in CHMI by adults 18–35 years of age and reported in Jongo et al.