Systemic inflammation
tumor necrosis
TUMOR NECROSIS
Ten ml of blood was collected from a vein in the antecubital fossa using standard venipuncture technique. The samples were allowed to coagulate for a minimum of 30 minutes at room temperature, then centrifuged at 12,000 rpm for 10 minutes. Serum was then collected and aliquoted into samples of 100°L and stored in a -80 degrees Celsius freezer. Analyses of C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor (TNF) levels was done in duplicates using immunofluorescent technique (CRP DuoSet ELISA kit; Human IL-6 Quantikine ELISA Kit; Human TNF-alpha Quantikine ELISA Kit, R&D Systems, Bio-Techne Corporation, Minneapolis, MN, USA).