T cell clonotypic analysis
T cell clonotypic analysis was performed by Adaptive Biotechnologies using their ImmunoSEQ hsTCRB sequencing kit on TRuC-T cell product or baseline and post-infusion PBMC samples. In brief, gDNA was isolated and amplified using multiplexed primers and sequenced on Illumina NextSeq. Data analysis of TCR clonotype and abundance was performed using ImmunoSEQ Analyzer (Adaptive Biotechnologies). Compare patient’s infusion product to their underlying repertoire to detect changes in clonality, expansion and persistence in post-infusion samples, peripherally.