NanoString
FFPE-derived RNA samples were analyzed on the NanoString platform using nCounter PanCancer Immune Profiling Panel (XT-CSO-HIP1-12; NS_CancerImmune_V1.1). Sample quality control was carried out as per the manufacturer’s instructions. Background levels were determined for each sample using mean plus two standard deviations of the included negative control counts. Background subtracted counts were normalized using geometric means of 27 housekeeping genes. log