Pre-trials
FLUID LOSS
Participants gave written informed consent and filled in an activity and health questionnaire (PAR-Q). Body mass and height were measured and, thereafter, a graded maximal oxygen consumption (Those meeting the pre-determined inclusion criteria (see below) attended another session in which fluid loss was estimated during 1 h of cycling to determine fluid intake for experimental trials. This was conducted under the same conditions as experimental trials, cycling at 55% 