Statistical analysis
Sample size was determined with a web-based calculator (The analyzed data were calculated as means ± standard deviations, medians, minimum and maximum values, interquartile ranges (IQRs; quartile 1 to quartile 3), or percentages, as appropriate. We used the Shapiro–Wilk test to check normality of distribution and Levene’s test to check equality of variances. To compare the two unpaired groups, we used the unpaired 