Experimental hut trials to assess insecticidal durability
To further assess the entomological superiority of InterceptorUntreated net—6 replicatesInterceptorPermaNetRoyal GuardInterceptorInterceptorPermaNetRoyal GuardInterceptorEach hut trial will last 9 weeks and will involve 9 consenting human volunteers sleeping in huts from dusk to dawn 6 days of each week of the trial. Treatments will be rotated each week using a randomized Latin Square Design to control for bias due to hut position and to minimize carry over effects between treatments. Sleepers will be rotated daily to control sleeper attractiveness to mosquitoes. On the 7th day of each week, huts will be cleaned and aired in preparation for the next rotation cycle. The trials will run for a total of 54 nights and replicate nets collected from the field will be rotated between huts on successive nights (one net per night). Nets will be made available for laboratory bioassays and chemical analysis as described earlier, immediately after they have been tested in experimental huts.The efficacy of each ITN hut treatment at each timepoint will be measured in terms of the following outcome measures:Percentage reduction in mosquito entry relative to the control (deterrence)Percentage of mosquitoes dead after 72 h,Percentage of mosquitoes exiting into the veranda trapPercentage reduction in mosquito blood-feeding relative to the control untreated netsPercentage reduction in proportion of fertile surviving blood-fed mosquitoes after dissection relative to surviving blood-fed mosquitoes in the untreated control hutWHO susceptibility bioassays will be performed to assess the susceptibility of the wild vector population at the Cove experimental hut station to the insecticides in the study nets during each hut trial.