2.3.4. Blood Sampling and Analysis
BLOOD, SEPARATION
Blood samples were collected eight times through each trial (at baseline, immediately before and after each exercise set, and 60 min after exercise completion) into 15 mL and 2.5 mL syringes via a cannula inserted into the antecubital vein. Immediately after blood sampling, 12 mL of blood was transferred into an ethylenediaminetetraacetic acid-containing tube (6 mL) or a serum separation tube (6 mL). Glucose and lactate concentrations in the remaining blood samples were measured immediately using a glucose analyzer (FreeStyle Freedom Lite; Nipro Corp., Osaka, Japan) and a lactate analyzer (Lactate Pro 2; Arklay Inc., Kyoto, Japan), respectively. Plasma and serum samples were obtained by centrifugation for 10 min at 4 °C (3000 rpm, 1712× The blood pH, bicarbonate (HCO