2.4. Fatty Acid Analysis
Erythrocytes (obtained by centrifugation of maternal blood and cord blood) and colostrum were stored at −80 °C until analysis. The sample (100 µL) was added to 2 mL methanol:hexane (4:1) solvent containing 50 µg/mL butylhydroxytoluene. The transmethylation method developed by Lepage and Roy was used [