DNAseq protocol
tumor
TUMOR
Indexed paired-end libraries of tumor DNA were performed using the Agilent Sureselect XT2 library prep kit. The kit supports sequencing targeted regions of the genome spanning 2.7 Mb. About 50 ng of input DNA were used to build the libraries according to manufacturer’s protocol. The pool was finally sequenced on a NovaSeq 6000 (Illumina) S2 × 150 bp flow cell.