Significance Statement
The inhibition of matrix metalloproteinase (MMP)9 attenuates memory consolidation and subsequent recall in a delay cue conditioning paradigm. However, it is currently unclear whether this is also the case for other learning scenarios that rely on a different neurocircuitry. We test this hypothesis in human trace fear conditioning which employs remote cues and is additionally dependent on hippocampus involvement. We find that doxycycline does not reduce fear retention in our preregistered analyses. Exploratory analyze might potentially suggest a weak effect of doxycycline on trace fear memory retention.