Stool sample collection
Stool samples were collected from all patients who were recruited with genetic testing sample collectors before and after treatment. Samples were collected only once from HC. All patients were required not to take antibiotics or probiotic preparations in the two weeks preceding the study, and they had to stop eating after 8 PM, before the collection of specimens. They then kept specimens until the next morning in a designated area in the hospital. After collection, specimens were stored at -80°C immediately until further processing. And the stool samples of 11 patients in the SLF group and the control group were randomly selected for subsequent intestinal flora sequencing.