Analysis of biochemical parameters
high-density lipoproteins, TG
STERILE, CLOT
For biochemical analyses, blood collection was performed by a nurse who used three different sterile tubes for venipuncture: tube 1 (with anticoagulant K3 EDTA-ethylenediamine tetraacetic acid), tube 2 (with anticoagulant sodium fluoride), and tube 3 (with clot activator). Samples in tubes 2 and 3 were immediately centrifuged to obtain plasma and serum, respectively. These were submitted for analysis less than 2 h after collection.Pre-and post-intervention analyses of the lipid profile were performed and included total cholesterol (TC), triglycerides (TG), high-density lipoproteins (HDL-C) and low-density lipoproteins (LDL-C), and fasting glucose and ultrasensitive C-reactive protein (us-CRP).For the determination of lipid fractions of total cholesterol (TC), HDL-C and TG were measured using enzymatic assay (Allain et al. Fasting plasma glucose was measured using the enzymatic method (Cooper, 