Biochemical evaluations
fasting blood glucose
INSULIN RESISTANCE, -20
At the beginning and end of the study, 5 cc blood samples were collected from all participants who had fasted for 10–12 h. Serum samples were obtained by centrifuging the blood samples at 3000 rpm for 15 min and immediately stored at -20 °C until testing. Using the Alpha Classic AT Plus analyzer, serum levels of fasting blood glucose, triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were evaluated by the colorimetric method. The serum insulin level was measured by the quantitative luminescence method using a diagnostic kit from “Snibe Company” (Shenzhen, China) and the Maglumi 800 analyzer in specialized laboratory. The triglyceride-to-glucose ratio was obtained by logarithmically multiplying the product of triglycerides and half of the fasting plasma glucose, and values greater than or equal to 4.49 were considered indicative of insulin resistance [