2.7. Lentivirus Infection
The specific siRNA of MAP3K1 and scrambled siRNA control were designed and synthesized by GenePharma (Shanghai, China). The shMAP3K1 plasmid was constructed and synthesized using the pSIH vector by GenScript Company (Nanjing, China). Lentivirus was packaged by cotransfection of psPAX2, pMD2.G, and shMAP3K1 plasmids into HEK293T cells. PBMCs were cultured in a 96-well plate in the presence of polybrene (1 : 1,000, Sigma-Aldrich), with the addition of 30 