Blood Glucose Levels
BLOOD
Blood glucose levels were monitored during the experiment. Thirty minutes after posttreatment with glibenclamide or placebo (T110) and when initial fasting glycaemia had declined by 10%, blood glucose concentrations were clamped around 4–7 mmol/L by 20% glucose infusion. Infusion rates necessary to maintain blood glucose after drug intake were registered throughout the experiment. Twelve blood samples were obtained for the determination of glucose during the experimental period. Blood samples were drawn prior to glibenclamide/placebo administration and then with 15–20-min intervals starting at T110. The venous blood samples were drawn from the intravenous catheter using a blood gas aspirator (Radiometer, SafePICO, self-filling blood gas syringe), and the blood glucose concentrations were determined with a blood gas analyzer (Radiometer, ABL90 FLEX). After finishing the measurements (T240), participants were provided with a standardized meal rich in carbohydrates which they had to consume before getting discharged.