3. Discussion
neurotoxicity, CML
CML
Cuban policosanol supplementation in Korean participants increased the serum HDL-C level and enhanced the HDL functionality to inhibit the oxidation and glycation of LDL and HDL [Many studies were carried out with different policosanol doses in different countries and ethnic populations, such as Caucasian [On the other hand, with the exception of Cuban policosanol [After 12 weeks of Cuban policosanol consumption, the systolic and diastolic BP were reduced significantly but still in the normal range of up to 7% (The hepatic functions were also improved remarkably by the policosanol consumption with lower serum AST, ALT, γ-GTP, and BUN levels (Adding CML to HDL for 48 h increased the production of yellowish glycated fluorescence with proteolytic degradation of apoA-I and the loss of paraoxonase activity [The CML also exhibited neurotoxicity and embryotoxicity in adult zebrafish and its embryo [Regarding the mechanism of action, policosanol consumption resulted in an increase in HDL-C and apoA-I content in HDL via CETP inhibition, as reported elsewhere [As summarized in A limitation of this study was that the data on lifestyle, such as diet consumption, smoking, and alcohol drinking frequency, exercise intensity, and time were obtained from self-reported questionnaires. The validation of these data for diet, smoking, drinking, and exercise might be intricate for distinguishing the consumption of policosanol or placebo effect. In addition, methodological and practical difficulties due to space limitation in rotor for ultracentrifugation were related with the selection of participants. To achieve more reliable and accurate results, the four steps of ultracentrifugation for a total of 96 h and subsequent dialysis for each 24 h, from VLDL, LDL, HDLIn conclusion, 12 weeks of Cuban policosanol (Raydel