Changes in predicted gut metabolites after Probio-X intervention.
hyperlipidemia
HYPERLIPIDEMIA
The probiotic treatment did not seem to drastically modulated patients’ fecal microbiota structure and composition; thus, we further investigated the mechanisms of regulation of hyperlipidemia by analyzing probiotics-driven changes in predicted gut metabolites from the MAGs. A total of 41 gut metabolites were predicted across 337 MAGs (selection criteria: integrity greater than 80, contamination less than 10, and relative abundance greater than 1%; data not shown). The gut metabolite, MGB043, was common to the majority of MAGs, while MGB034, MGB055, MGB056, and MF0059 were predicted to be present exclusively in only one MAG. A total of 20 gut metabolites were predicted from the MGB database, including the major metabolites MGB011 and MGB043 (Predicted gut metabolites. (A and B) Relative abundance of predicted gut metabolites. (C) Violin plots showing differential gut metabolites. Correlation heatmap of (D) metagenome-assembly genome (MAGs) and gut metabolites; (E) MAGs and blood indicators. Red indicates positive correlation, blue indicates negative correlation, and the color intensity is the strength of correlation. *, Correlation analysis revealed significant positive correlations between three predicted differential gut metabolites (cortisol degradation, glycolysis, and pentose phosphate pathway) and almost all differential MAGs (Spearman's test, 