IgG binding affinity measured by Carterra
A medium density HC30M sensor chip was preconditioned with 1-min pulses of each of three solutions: 50 mM NaOH, 1 M NaCl, and 10 mM Glycine pH 2.0. After preconditioning, the chip was activated with a 10-min injection of a freshly prepared 1:1:1 (v/v/v) mixture of 0.4 M 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), 0.1 M 