2.9. Cell Proliferation Assay
infection
INFECTION
The procedure for lentivirus infection and coculture has been described above. Then, the cells were harvested and stained with phycoerythrin (PE)-CF594-conjugated antihuman CD4, antihuman CD8 PerCP/Cy5.5, and eFluor™ 780 (eBioscience, CA, USA). In mice, single-cell suspensions of spleens were prepared and stained with antimouse CD4 FITC, PE-Cy7-conjugated antimouse Ki67 (eBioscience, CA, USA), eFluor™ 780, antimouse CD3a BUV395, and antimouse CD8a BV510 (BD Pharmingen, USA). Data were collected by BD LSRFortessa™ Flow Cytometer and analyzed by FlowJo software.