Measurements
TG
SEPARATION, CREST
Demographic characteristics, including age, sex, marital status, medical history, level of education, household status, and current drugs use were collected from each participant by completing a general questionnaire. Anthropometric variables of each patient including weight, height, body mass index (BMI), WC, and blood pressure were measured at the beginning and at the end of the study, after an overnight fast. Body weight was measured using a calibrated hand scale to the nearest 0.1 kg while the subjects wearing minimal clothing and no shoes (Seca, Germany). The scale was calibrated daily by a five-kilogram weight. Height without shoes in the standing position, while shoulders were in a normal position was measured using a stadiometer to the nearest 1 cm (Seca, Germany). Then, BMI was calculated as body weight in kilograms/body height in meters squared. WC was measured between the lower rib margin and the iliac crest at the end of normal exhalation, without any pressure to body surface using an inelastic tap, to the nearest 0.1 cm. an Experienced nutritionist measured blood pressure using a standard hand-held sphygmomanometer (ALPK2, Zhejiang, China; Datis Co, Tehran, Iran) over the right arm for each person, twice after the individuals had been sitting for 15 min. The average of two measurements was considered as the final blood pressure.At the beginning and at the end of the intervention, a 10 cc venous blood sample was taken from participants after overnight 10–12 h fasting in the endocrine and metabolism research center laboratory (Esfahan, Iran). After separation of serum samples from whole blood, all aliquots were stored at −80 °C until biochemical analysis time. Serum insulin levels were measured using the (enzyme-linked immunosorbent assay) ELISA kit (Pars Azmoun kit, Tehran, Iran). FBG, concentration of serum cholesterol total (TC), TG, low-density lipoprotein cholesterol (LDL-C), and HDL-C were determined using the colorimetric technique by available standard kits (Pars Azmoun kit, Tehran, Iran). Also, LDL-C/HDL-C and cholesterol/HDL-C ratios were calculated. HOMO-IR was determined using the following formula: HOMA-IR = fasting glucose (mg/dl)*fasting insulin (μU/ml)/405