RT-qPCR
Total RNA was extracted from cells using the Total RNA Isolation Kit V2 (Vazyme, China), and Complementary DNA (ctDNA) was synthesized by the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme, China). In addition, RT-qPCR was conducted on an ABI 7500 thermocycler (Applied Biosystems, Foster City, CA, USA) using the SYBR Green PCR Master Mix (Vazyme, China), with appropriate primers were listed in Additional File 2. The relative expression levels of mRNA were reported as fold change compared to levels detected in controls via the ΔΔCt method.