Interleukin-1-beta (IL-1β)
peripheral white blood cells
TSD
IL-1β in peripheral blood was analyzed on day 1 (predose, 2 h and 8 h post dose), on day 2 (24 h after the initial dose of JNJ-54175446), and on day 4 during TSD (predose, 2 h and 8 h post dose). Venous blood samples were taken (TruCulture) to obtain peripheral white blood cells (WBCs) for ex-vivo stimulation using LPS/BzATP to stimulate IL-1β release. At each sampling, 1 mL blood was collected in each of two TruCulture tubes containing 100 ng/mL LPS (cat#782-001087, Myriad RBM, Austin, Texas,USA). The tubes were incubated at 37 °C for 1 h in dryblock heaters followed by addition of 2′3′-BzATP (NuBlocks, Oceanside, California, USA) to one of the tubes to a final concentration of 1 mM (using 100× stock solution). The contents of both the tubes were mixed by inversion and incubated for an additional 1.5 h. The tubes were then centrifuged (1000 