Gene expression analysis
We have evaluated the changes in the expression of different immunity-related genes listed in the table below, before and after intervention.StepOne Plus thermal cycler was used in this study, and the thermal profile was as follows: 94°C for 2 min as pre-PCR and 95°C for 30 sec, 62°C for 45 sec, and 72°C for 45 sec for 35 cycles. Followed by 72°C for 10 min as post-PCR step. The 2
