Biological specimen collection
ascites, Peripheral venous blood samples, Cancer
ASCITES, METASTASES, CANCER
Peripheral venous blood samples and stool samples will be collected before the start of the treatment. Additionally, peritoneal metastases samples and ascites samples (100 ml) will be collected at the time of 1st PIPAC procedure.All collected samples will be prepared according to standard laboratory protocols. Plasma and serum samples will be aliquoted in four 1ml tubes and stored at -80 C° in the laboratory at National Cancer Institute. Fresh stool samples will be split into four tubes containing at least 1 g of content and stored at -80 C° in the same laboratory. Gut microbiome analysis will be performed from stool samples by 16 S sequencing in the current trial. Also, biological specimens may be used for future studies.