Effects of 
OBESE
As differences in passive force between lean and obese ZSF1 rats were still present in the presence of PKA, we investigated whether the beneficial effect of GGA on cardiomyocyte stiffness was linked to altered expression of titin, as changes in titin isoform expression can impact cardiomyocyte compliance. We quantified the expression of the stiff and compliant titin isoforms in lean and obese ZSF1 rats treated with either vehicle or GGA. No statistically significant differences were observed between the groups for the N2BA1 (Figure No differences in the major titin isoforms or the ratio of their expression relative to the total titin pool were observed among the groups. (a) Raw image of titin blot; (b) lane profiles for each of the raw titin blots. The analysis area is depicted by the corresponding colored rectangles in A; (c) expression of the N2BA1 isoform relative to total titin; (d) expression of the N2BA2 isoform relative to total titin; (e) expression of the N2B isoform relative to total titin; (f) the ratio between the total N2BA (N2BA1 + N2BA2) and N2B isoforms. Data are expressed as mean ± SEM. A one‐way ANOVA with a Bonferroni's post hoc test was utilized to assess differences between the groups.