Analysis of circulating immunocytes of peripheral blood
Circulating immunocytes, such as MDSCs, cluster of differentiation (CD) 4 T‐cells, CD8 T‐cells, and Tregs, were analyzed. To analyze circulating immunocytes, peripheral blood samples of patients were collected at four points (before and after induction chemotherapy, 4–8 weeks after the start of maintenance treatment, and after maintenance treatment). The peripheral blood samples of patients were centrifuged at 3000 rpm, and peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation within 6 hours of blood collection. The collected PBMCs were stained by antibodies for surface markers.The percentage of each subset of PBMCs was counted by flow cytometry. The number of each subset of PBMCs was calculated by multiplying the percentage of each subset of PBMCs by the total number of granulocytes. MDSCs are commonly defined as a CD11b