Lung distribution study in mouse
bleeding, ’s lungs
BLEEDING
Male ICR mice were randomly divided into two groups (21 mice each) and given a single oral dosage of nelfinavir mesylate at 250 or 750 mg/kg (via gavage). By bleeding from the orbital sinus, mice under isoflurane anesthesia were killed at 0.5, 1, 3, 6, 9, 12, and 17 h (three mice per time point) after dosing. The blood samples were collected into heparinized tubes, and the plasma fractions were obtained by centrifugation. The mouse’s lungs were removed, cleaned in ice-cold saline, blotted, weighed, and homogenized in four-fold volumes of ice-cold water. In order to preserve the samples for liquid chromatography/mass spectrometry analysis, the mouse plasma and lung homogenate samples were kept at −70 °C.