Sorting of VRC 018 B cells from donor N751 reveal that only a small fraction of elicited antibodies recognize the glycan-dense surface of the Env trimer
In the Vaccine Research Center (VRC) 018 clinical trial (To quantify the number of cells binding to the glycan-dense Env-trimer surface relative to those bound the glycan-free base, we performed cell cytometry on immunoglobulin G-positive (IgGFrom two plates of single-sorted B cells, 96 wells each, specific for the glycan-base BG505 trimer, we sequenced immunoglobulin genes and cloned them into an expression vector utilizing a method for rapid assembly, transfection, and production of immunoglobulins (RATP-Ig).