Imaging Protocol
STERILE, ANTERIOR, BLOOD
Labeling of For labeling, 150 µL sterile phosphate-buffered saline (PBS) was added to a vial with ZHER2:41071. The vial content was mixed and checked visually to ensure that the content was completely dissolved, and no particles or opalescence was observed.The The yield was 97 ± 1%. The injected activity was 451 ± 71 MBq. Monitoring of safety, thorough assessment of vital signs, ECG and physical examination were performed during imaging visits (0-24 h after injection) and 3-7 days after injection.Imaging was performed using a Siemens Symbia Intevo Bold scanner equipped with a high-resolution low-energy collimator. Anterior and posterior planar whole-body imaging (at a scan speed of 12 cm/min, 1024 × 256 pixel matrix) was performed at 2, 4, 6 and 24 h after injection of Blood samples were obtained 5, 10, 15, 20, 30 and 50 min after injection if the patient's condition permitted. Small (500 µL) aliquots were taken, and activity was measured using an automated gamma-spectrometer with a NaI (TI) detector (Wizard 1480, Perkin Elmer, Waltham) to determine the activity in blood during the distribution phase.Dosimetry was evaluated as described in Maximal standard uptake values (SUV