Tacrolimus
BLOOD
Blood samples were obtained from all subjects in parallel as for plerixafor. Whole blood from each sample was stored in −80°C condition until shipment. The analysis of K2EDTA whole blood for the quantitation of tacrolimus using a validated method by LC-MS-MS was performed by Worldwide Clinical Trials, Bioanalytical Services (Austin, TX, USA) in compliance with principles of GLP standards as referenced above.Study data were collected and similar pharmacokinetic parameters as above were calculated using Phoenix WinNonlin, Version 8.0.0.3716, Certara, St. Louis, MO, software and evaluated with Watson Laboratory Information Management System (LIMS; Version 7.2.0.03, Thermo Fisher Scientific) software.