2.7. Biochemical Parameters
occult blood reaction
BLOOD
Blood pressure, pulse rate, and body weight were measured at weeks 0, 8, and 12. The concentrations of biochemical parameters were also measured at weeks 0 and 12. Blood samples were drawn from each participant after 4 h of no food or drink except water (fasting) prior to testing. Biochemical parameters included hematological tests (white blood cell count, red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelet count, leukogram), biochemical tests (total protein, albumin, total bilirubin, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase (IFCC), alkaline phosphatase (IFCC), gamma-glutamyltransferase, urea nitrogen, creatinine, uric acid, sodium, chlorine, potassium, calcium, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides, phospholipids, glucose, HbA1c (NGSP), insulin), and urinalysis (protein, sugar, bilirubin, urinary ketone bodies, occult blood reaction, urobilinogen, pH, and specific gravity). In addition, serum BDNF was quantified. All of these tests were performed at LSI Medience Corporation (Tokyo, Japan).