2.5. Evaluation of Hematological Indices
inflammation
INFLAMMATION, OXIDATIVE STRESS
To investigate the influence of exercise load and LC-Plasma intake on the objective parameters, hematological indices known to be associated with exercise-induced inflammation and oxidative stress were measured. The levels of transforming growth factor-β (TGF-β) and interleukin-6 (IL-6) were measured using an ELISA kit (R&D systems). The levels of cathepsin L, adrenaline, hydroxy-8-deoxyguanosine (8-OHdG), testosterone, and leptin were measured using separate ELISA kits (R&D systems, Minneapolis, MN, USA; R&D systems; Arigo Biolaboratories Corp, Hsinchu City, Taiwan; Northwest Life Science Specialties, Portland, OR, USA; and R&D systems, Cosmo Bio, Tokyo, Japan, respectively). The levels of creatine phosphokinase (CPK) were measured by Hoken Kagaku, Inc. (Kanagawa, Japan) using the Japan Society of Clinical Chemistry standardization method.