Enzyme linked immunosorbent assay (ELISA) for the validation of proteomic data
MMP-9 (cat. no. MBS2880173), ACVR1B (cat. no. MBS7232818), and FETUB (cat. no. MBS454400) concentrations in the plasma samples were measured according to the manufacturer’s specifications using MyBioSource ELISA kits. The minimum levels of detection were as follows: WFDC3, 3.12 ng/mL; FGFR1, 0.094 ng/mL; MMP-9, 0.056 ng/mL, ACVR1B, 0.5 ng/mL, FETUB, 0.04125 ng/mL.