Ultradeep NGS sequencing
tumor, UMI
TUMOR
The DNA samples from tumor tissue were ligated with regular adapters. Target capture of tissue DNA was performed using a commercial panel which consists of 520 genes (OncoScreen Plus), spanning 1.64 Mb of the human genome. The detailed genes were listed in Additional file The cfDNA from peritoneal lavage were ligated with UMI-containing adapters. Oligonucleotides corresponding to the UMI adapters were designed and patented by Burning Rock Biotech (China). The oligonucleotides were commercially synthesized, annealed and purified following standard protocols in previous study [