IFNγ secretion at clinical lot release
An aliquot of fresh cells was taken on the day of collection and tested using the plate-based stimulation assay described above (24 h stimulation). Controls consisted of ‘mismatch’ peptide–HLA-coated plates. The supernatant was collected and analysed using IFNγ ELISA kits (Quantikine ELISA Human IFNγ Immunoassay kit, R&D Systems) or using an ELLA Simple Plex Immunoassay platform (Simple Plex for Human IFNγ, Bio-techne). EC