Ethics approval and consent to participate
Informed consent was obtained from all participants prior to inclusion in the study. The study was conducted in accordance with the principles and all the subsequent amendments of the declaration of Helsinki. The manuscript does not contain any identifiable information or images. The Swedish Ethical Review Authority in Uppsala has approved this study with diary number 2018–568 and diary number 2019–00133. Further, the clinical trial has been registered at ISRCTN number:73435627; 10.1186/ISRCTN73435627 .