Serum concentrations of the cardiovascular risk factor profile
Fasting blood samples are collected and centrifuged at 1800 g for 10 minutes, directly on site or after transport on the same day. Serum is frozen at − 80 °C until assessment. In all serum samples, total cholesterol, HDL-cholesterol, triglycerides, insulin, and IGF-I were assessed by the Department of Clinical Chemistry of the Amsterdam UMC.