3.2. Validation of the EV Extraction Method
To validate the extraction method, Western blots were performed to detect EV-typical proteins (Currently, there are no specific singular EV markers for EV characterization but the International Society of EVs recommends the use of cell-membrane-dependent and transmembrane proteins like CD63, CD81 or CD82 or cytosolic proteins like ALIX and flotillin, which are connected to the endosomal sorting complex required for transport [