Outcome variables 
gingivitis, tooth
PLAQUE, GINGIVITIS
The clinical outcomes were assessed at every visit, measuring:Plaque levels; assessed using the Plaque Index (PI) at six sites per tooth after using a disclosing solution of Tri Plaque ID Gel (GC Malaysia, Kuala Lumpur, Malaysia) [Severity of gingivitis; assessed by the Periodontal inflamed surface area (PISA) at six sites per tooth [Prior to the study, the examiner (NFA) was trained and calibrated for accuracy and repeatability using the PI and PISA in five individuals who were not participating in this study. Training and calibration of the examiner were conducted by an experienced periodontist (BB). Inter- and intra-examination Cohen’s Kappa scores showed a percentage of agreement of > 85% in both parameters.