Lipoaspiration
STERILE, INFILTRATED
Prior to surgical intervention, coagulation profiles and serum chemistries were performed. Under general anesthesia, the liposuction site (abdomen) was infiltrated with 250 mL of standard wetting solution consisting of 1000 mL of Ringer’s lactate to which was added 50 mL of 1% lidocaine with epinephrine. After waiting 15 minutes for the hemostatic effect of epinephrine to take effect, 200-300 mL of lipoaspirate were collected using a 3-mm blunt-tip Mercedes cannula. The lipoaspirate was drawn directly into a sterile processing canister (GID SVF-1, Louisville, CO, USA; Graphic explaining SVF cell processing technology using the GID Bio SVF-2 device. Collection and digestion of lipoaspirate take place within a closed-system sterile device The GID SVF-2 device (GID Bio, Louisville, Colorado) is a single-use, sterile, closed-system for the collection and collagenase processing of lipoaspirate within a chamber located above a 400 nm mesh filter. Subsequent centrifugation at 600