4.9. Malassezia Species Identification by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
Agar plates of 5.5 cm in diameter with modified Dixon agar medium (Tritium Microbiology B.V., Eindhoven, The Netherlands) were pressed against the skin for 20 min. Plates were transferred to the Alrijne Hospital (Leiden, The Netherlands) and cultured for up to 21 days at 33 °C. If mycological growth was observed, an isolate was taken and frozen in microbank 2D tubes (pro-lab diagnostics, Richmond Hill, ON, Canada). Isolates were transported to the Westerdijk Fungal Biodiversity Institute (Utrecht, The Netherlands), defrosted, and further cultured on modified Leeming and Notman medium before analysis using matrix-assisted laser desorption ionization–time of flight mass spectrometry, as described by Kolecka et al. [