4.8. Western Blotting Analysis Applied to CX3CR1 in Patients’ Peripheral Blood, Both before and after OncoTherad
Peripheral blood samples were collected from 20 patients, both before and after OncoTheradNitrocellulose membranes were blocked with 3% bovine serum albumin (BSA) solution diluted in TBS-T buffer to avoid non-specific protein binding. These membranes were incubated with primary antibodies, such as anti-CX3CR1 (dilution: 1:1500 in 1% BSA) and anti-β-actin (dilution: 1:1500 in 1% BSA), at 4 °C overnight (Bands’ immunoreactivity was visualized by incubating them with DAB (diaminobenzidine) chromogen (Sigma Chemical Co., St. Louis, MO, USA). Samples collected from all 20 patients, both before and after OncoTherad