2.7. Sequencing Analysis of Bacterial 16S RNA Genes
DNA isolation from feces and sequencing of the V3–V4 regions of the 16S rDNA gene were performed using the Illumina MiSeq instrument (Illumina INC, San Diego, CA, USA) at the Institute of Clinical Molecular Biology, of the University of Kiel (Kiel, Germany) according to their own protocol. DNA was isolated using microcentrifuge columns with silica membrane. Extracted DNA was purified using an Agencourt AMPure