3.7. Effects of BB536 Intake on the IFN Response in Plasma Ex Vivo
To accurately determine the cytokines in plasma, the cytokine concentrations of IFNα2a, IFNβ, IFNγ, IL-10, IL-12/IL-23p40, IL-12p70, IL-15, IL-23, and MCP-1 were evaluated using the ECL-based U-PLEX assay method because of the high sensitivity of this immunoassay in detecting the trace amount of target molecules in the sample. Even so, the measurement values of IFNα2a, IFNβ, IFNγ, IL-10, IL-12p70, and IL-23 were below the limit of quantitation according to the manufacturer’s instructions. All of the measurement values of IL-12/IL-23p40, IL-15, and MCP-1 were within the range of values obtained from the standard curve, and these data were used for further statistical analysis.As shown in 