Statistical analyses
Statistical analyses were performed by SPSS version 23.0 (IBM Corp, Armonk, NY, USA) and GraphPad Prism version 8.0 (San Diego, California, USA). Continuous data were described as means ± standard deviation, and inter-group comparison was performed with a t-test or analysis of variance. All continuous data were abnormally distributed. Categorical data were described as n (%), and inter-group comparisons were analyzed by χ