DNA isolation
-20
Venous blood from HBV and HDV positive patients were used for studies. HBV DNA and HDV RNA were isolated using the GeneJET Viral DNA/RNA Purification Kit (Thermo Fisher Scientific™, USA) according to the manufacturer’s instructions. 200 µl of venous blood with EDTA was used for isolation. cDNA from RNA was obtained using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, USA) according to the manufacturer’s instructions. DNA/cDNA was stored at -20