Mouse monitoring
weight loss, hunching, paralysis
Animals were monitored daily and always immediately killed as soon as a cumulative clinical score of 3 or above was reached, based on weight loss (score 1, >10–20%; and score 2, >20% or >15% if maintained for >72 h), posture (score 1, hunching noted only at rest; and score 2, severe hunching), activity (score 1, mild to moderately decreased; and score 2, stationary unless stimulated, hind limb paralysis) and white cell count (score 1, 10–60 × 10