Mosquitoes and mosquito collection
HDN, a mouth
KU’s HLCs were conducted for 45 min during each hour of the six-hour replicate, allowing for a 15-min break per collection hour. To conduct the HLCs, a collector sat on a chair in the centre of the temporary structure and collected mosquitoes with a mouth aspirator as mosquitoes landed on the lower leg between the knee and the ankle, before the mosquitoes were able to bite (Fig. The HDN consists of a person sitting underneath two untreated, white nets: the first net is sealed against the ground and protects the human from direct mosquito exposure, and the second, larger net is placed directly over the inner net (Fig. 