2.5. Quantitative Real-Time PCR and Data Analysis
RT2 Profiler PCR Arrays were used (Qiagen, The Netherlands) to analyze CAT (GenBank accession No. NM 001752), SOD1 (GenBank accession No. NM 000454), CCL5 (GenBank accession No. NM 002985), PPARγ (GenBank accession No. NM 001145366), NFKB1 (GenBank accession No. NC 000004.12), VDR (GenBank accession No NT 009526.11), MIF (GenBank accession No. NC AY004865). Actin β ACTB (NM_001101) was used as a housekeeping gene. Each qRT-PCR experiment was performed in triplicate and repeated at least twice according to the manufacturer’s instructions (Qiagen, The Netherlands) [