2.5. Measurement of Hepatic Glycogen
BEST
On day 15 at 6:30 a.m. after an overnight fast, hepatic glycogen was determined by 13C-MRS. All 13C-MRS experiments were performed on a 3T MR system (Achieva 3T-X Philips Healthcare, Best, The Netherlands) using a 21 × 24 cm 13C quadrature detection surface coil (RAPID Biomedical GmbH, Rimpar, Germany). Hepatic glycogen levels were measured as described previously [