Molecular analysis
oncocytic follicular neoplasm, tumor
TUMOR, ATYPIA OF UNDETERMINED SIGNIFICANCE
For MD, total nucleic acid (DNA and RNA) was isolated from tumor cells scraped off cytology slides or from microdissected cytology cell blocks.CNA-LOH analysis was performed using the custom AmpliSeq NGS genome-wide LOH v2 panel, which assesses LOH and other chromosomal imbalances using 1500 single nucleotide polymorphisms, evenly distributed across all autosomes and the X chromosome. Dependent on the cytological classification, the three NGS panels were applied according to a predefined flowchart (Study flowchart. At the discretion of the dedicated thyroid pathologist who performed a blinded review of the cytology smears to select those with the highest cellularity for MD, this included cytology suspicious for a oncocytic follicular neoplasm (O-FN) as well as cytology with atypia of undetermined significance (AUS) with suspicion of oncocytic cells.