Proliferation assay
PROLIFERATION
A real‐time proliferation assay was performed to observe the effect of LEAP‐purified pEVs on fibroblasts. NHDFs at 5000 cells per well were seeded on 96‐well E‐plates (Agilent Technologies, USA) in a complete growth medium containing 2% v/v serum, then placed in an xCELLigence RTCA eSight instrument (Agilent Technologies, USA) and their growth monitored overnight. Cells were washed, media was replaced with basal media containing 0.1% v/v serum, and their growth was monitored overnight. Cells were then treated with 15 μg/mL of LEAP‐isolated pEVs (h 0), incubated at 37°C with 5% CO