4.6. RNA Extraction, cDNA Synthesis and Microarray
In eight consecutive patients from the clinical trial, four from the CEE/MPA group and four from the ETotal RNA from each sample was used in the standard protocol from NuGen (San Carlos, CA, USA) to label targets. The RNA was reverse transcribed by Affymetrix (Santa Clara, CA, USA) in vitro into single-stranded sense target cDNA, and 1.6 µg per sample was hybridized to Gene Chip