Data collection and outcome measures
headache, flushing
Vascular parameters (superficial temporal artery [STA] diameter and radial artery [RA] diameter measured by high-resolution ultrasonography; facial flushing measured by speckle contrast imager), heart rate and headache were documented before Lu AG09222 or placebo infusion and PACAP38 or saline infusion at visit 1 and before VIP or saline infusion at visit 2. After PACAP38, VIP and saline infusions, vascular parameters, heart rate and headache were documented every 10 min from 0 to 2 h. After the observation period, participants were provided with a headache diary and discharged from the clinic. The diary was filled out every hour from 3 to 8 h (or until sleep) after the start of infusion with PACAP38, VIP, or saline, then at 24 h and daily thereafter until 1 week after the VIP or second saline infusion (if applicable). Participants recorded the presence of a headache and evaluated headache intensity, characteristics and localization, facial flushing, other associated symptoms and use of acute medication.The primary outcome measure was the area under the curve (AUC) of the change in STA diameter from 0 to 120 min after start of PACAP38 infusion (AUC