ELISpot and ICS analyses of PBMCs
ELISpot was performed as described previously (For ICS, HIV-1-specific CD4+ and CD8+ T cell responses were measured by a validated flow cytometry assay similar as previously described (Combinatorial Polyfunctionality Analysis of Single Cells (COMPASS) is a computational framework for unbiased polyfunctionality analysis of antigen-specific T cell subsets (