Evaluation of the biomarkers
Tumor necrosis
TUMOR NECROSIS, SENSITIVITY, OXIDATIVE STRESS
The extractions will be made one week before initiation of the program and one week after completing the intervention.Oxidative stress and antioxidant enzymes: Determination of oxidized lipids by the thiobarbituric acid reactive substances (TBARS) (fluorimetry) technique. Determination of oxidized proteins by the Advanced Oxidation Protein Products (AOPP) (colorimetry) technique. Determination of antioxidants by the total thiols technique (colorimetry). Determination of nitrites and nitrates (colorimetry).Antioxidant Enzymes: Superoxide dismutase (SOD), catalase (CAT), glutathion peroxidase (GPx).Inflammatory markers: Interleukin 6 and 10 (IL-6 and IL-10). Adiponectin. Tumor necrosis factor (TNF)-alpha. Serum IL-6 and IL-10 inflammatory markers and TNF-alpha will be determined with Luminex-100 IS (Integrated system: Luminex Corporation, Austin, TX, USA) system using the multiplex Linco High Sensitivity Human Cytokine Panel Lincoplex assay kit (Linco Research, Inc., MO, USA). Adiponectin will be determined with the Luminex-100 IS (Integrated system: Luminex Corporation, Austin, TX, USA) using the multiplex Linco Human Gut Hormone panel assay kit (Linco Research, Inc., MO, USA).