Gating strategy for the identification of monocytic myeloid-derived suppressor cells (M-MDSCs) and monocyte subsets
Peripheral blood was labeled with CD45, CD14, CD16, CD66b, CD335, and HLA-DR. After exclusion of doublets from the cell gate, granulocytes, T cells and NK (CD66b+CD335+CD14−), and basophils, lymphocytes and granulocytes (CD14-HLA-DR-) were excluded from the Monogate. Monocytes have been classified into three subtypes: classic (CD14+CD16−), intermediate (CD14+CD16+), and nonclassic monocytes (CD14dimCD16+). M-MDSC subsets were defined as CD14+ and HLA-DRlow cells. (Gating strategies are represented in Additional file 