Immunologic correlatives: human T cell 
Peripheral blood mononuclear cells (PBMCs) were isolated from patient apheresis cones. Informed consent was obtained from all donors for the use of their sample for research purposes. CD3+ T cells were isolated using a magnetic sorting kit (Miltenyi Biotech) and activated using CD3/CD28 beads (ThermoFisher, cat #11161D, RRID : AB_2916088).Autologous monocyte-derived dendritic cells were matured by isolating CD14+ cells via magnetic sorting (Miltenyi Biotech), followed by incubation with human GM-CSF (800 U/mL) and IL-4 (1000 U/mL). On Days 3 and 5, media was replaced with human GM-CSF (1600 U/mL) and IL-4 (1000 U/mL). On Day 7, non-adherent cells were collected, washed with PBS, and resuspended in medium containing GM-CSF (800 U/mL), IL-4 (1000 U/mL), TNF-alpha (1100 U/mL), IL-1beta (1870 U/mL), IL-6 (1000 U/mL), and PGE2 (1ug/mL).Two days later, dendritic cells were either transfected with pCDNA3.1 expression plasmids (NO insert, TYRP1, GP100 or OVA) (1µg/10T cell responses to antigens of interest were defined as 1) at least a doubling of the number of spots formed in post-treatment versus pre-treatment, and 2) no overlap of the 95% confidence intervals of pre- and post-treatment responses.