Study design and supplementation regimen
The Institutional Review Board at the North Carolina State University reviewed the present investigation for ethics. The study was a randomized, double-blind, crossover with a 7-day washout period. Participants completed a preliminary familiarization session (day 0), followed by ingestion the next day (day 1) of either supplement or placebo as provided. On days 1–6 of supplementation, participants ingested the supplement in the morning with breakfast, and on day 7 (the experiment day) they ingested the supplement 1 h prior to completing the exercise protocol. This timing is consistent with literature and data indicating a peak concentration approximately 1 h after ingestion [The supplement and placebo (see Supplement and placebo formulation.1.2:1:1 ratio of leucine, isoleucine, valine2.Overage included to ensure target amounts were met.3.Negligible difference in total weights due to absence of bitterness masker in the placebo.