Plate-based antigen stimulation of neoTCR cells
Streptavidin-coated plates (Eagle Biosciences) were pre-incubated with cognate or control peptide–HLA molecules at various concentrations for 2–5 h at room temperature or 16–30 h overnight at 4 °C. T cells electroporated with neoTCRs or control TCRs were then stimulated on the plates in T cell culture medium (TexMACS supplemented with 3% human AB serum and 1% penicillin–streptomycin, Gibco) at 37 °C and 5% CO