Whole-exome capture and sequencing
Two hundred nanograms of genomic DNA was used for library preparation, using the Agilent SureSelectXT Reagent Kit. The totality of the enriched library was used in the hybridization and captured with SureSelect All Exon v5 or v6 (Agilent) baits. After hybridization, the captured libraries were purified according to the manufacturer’s recommendations and amplified by polymerase chain reaction (12 cycles). Normalized libraries were pooled, and DNA was sequenced on an Illumina NextSeq 500 device using 2× 111-base pair (bp) paired-end reads and multiplexed.