Plasma analysis
mineral deficiency, ®
MINERAL DEFICIENCY
Analysis to quantify mineral abundances in plasma samples drawn at baseline and 6-months was conducted by the Jay Turner Group in the McKelvey School of Engineering at Washington University. Samples were digested with microwave assistance based on a protocol adapted from Harrington et al., to optimize recovery of iron, selenium, and zincMineral concentrations were measured using inductively coupled plasma mass-spectrometry (ICP-MS; NexION® 2000, Perkin-Elmer), using standard operating conditions (Supplementary Table Standard reference ranges and cutoffs were used to determine prevalence of mineral deficiency (Supplementary Table 