Procedures
liver stiffness, TG, steatosis, Triglyceride-glucose
LIVER FIBROSIS, LIVER, VIRAL HEPATITIS, INSULIN RESISTANCE, BLOOD, STEATOSIS
Blood samples were collected after 8 to 12 h of fasting. Lipid profile, fasting blood glucose, liver enzymes, and serum creatinine were measured by Pars biochemical kits using the photometric method. HbA1c was measured using capillary method. Markers for viral hepatitis were also assessed.Antinuclear antibodies (ANA) was using immunofluorescence method. ELISA was used to measure thyroid hormones. Triglyceride-glucose index (TyG index), a measure of insulin resistance (IR), was calculated using the formula: (TyG) = Ln [FBS (mg/dl) × TG (mg/dl)/2] [Liver elastography was performed at baseline and the end of the study. Liver fibrosis was defined according to the liver stiffness measurement (LSM).Hepatic steatosis was measured based on the controlled attenuation parameter (CAP) using Fibroscan® 502 Touch equipped with both M and XL probes. The procedure was performed by an expert physician (blinded to the study protocol) trained and certified by the Iranian Association of Gastroenterology and Hepatology (IAGH). Participants with CAP greater than 302 dB/m were enrolled in the study.