PBMC isolation
SEPARATION
After blood sampling in EDTA tubes, PBMCs were isolated from the whole blood by density gradient centrifugation (Lymphocyte Separation Medium, CMSMSL0101, Eurobio) with SepMate tubes (85460, STEMCELL Technologies). Whole blood was transferred into SepMate tubes at a rate of 17 ml of whole blood per tube and then centrifuged at 1,200