Inflammatory biomarker
MP, ®
TUMOUR NECROSIS
Serum samples were used to determine circulating concentrations of the cytokines interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17A, interferon-gamma (IFNγ), and tumour necrosis factor alpha (TNFα), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1 beta (MIP-1β), granulocyte colony-stimulating factor (G-CSF), and granulocyte monocyte colony-stimulating factor (GM-CSF) in serum samples using the Bio-Plex® Pro Human Cytokine 17-plex Assay kit (Bio-Rad, Hercules, CA, USA) in the MAGPIX™ Multiplex fluorescence reader and Bio-Plex Manager™ MP software (Luminex Corporation, Austin, TX, USA). High-sensitive C-reactive protein (CRP) was measured in serum with an automatized ultrasensible turbidimetric method (AU2700 Chemistry Analyzer, Olympus Corp., Japan).