Ethical aspects
The TransAmigas project was approved by the Research Ethics Committee of the University of California, San Francisco (USSF), United States (16–19922), on October 4, 2016, and the Brazilian National Research Ethics Commission (CAAE: 61338116.0.0000.5375), on July 13, 2017. Potential participants were previously interviewed to confirm their eligibility and informed about the planned procedures before signing the informed consent form, thus, they had the right to withdraw from the study at any time, without any losses.