2.6. Analysis of Inflammatory and Oxidative Stress Biomarkers
Neurodegenerative Disease
BLOOD, NEURODEGENERATIVE DISEASE, EMD
Cytokines. Plasma samples collected on D1, D15 and D30 were used to assess the levels of cytokines using the multiplex bead-based immunoassay Luminex system using the MilliplexErythocyte sedimentation rate (ESR): Inflammatory conditions were monitored though the ESR test using the Sedi-Rate ESR System (Globe Scientific Inc., Mahwah, NJ, USA) following the manufacturer’s instructions on blood samples (approximately 5 mL) collected in VacutainerC-reactive protein (CRP): CRP was quantified in plasma samples collected on D1, D15 and D30 using the Human Neurodegenerative Disease Magnetic Bead Panel 2 (EMD Millipore; Billerica, MA) following the manufacturers’ instructions. Data was analyzed using the Luminex xPonent 3.1 software. White blood cell (WBC) count differential test: Blood samples (approximately 5 mL) collected on D1 and D30 in VacutainerReactive oxygen species (ROS): ROS levels were quantified in RBC collected on D1, D15 and D30 with a fluorometric assay based on the oxidation of the fluorochrome 2,7-dichlorofluororescein-diacetate (DCFH-DA, Sigma-Aldrich, St. Louis, MO, USA), as previously reported with modifications [