Laboratory analysis
respiratory syncytial virus A, parainfluenza
ADENOVIRUS, PARAINFLUENZA, RHINOVIRUS, ENTEROVIRUS, INFLUENZA B, INFLUENZA A
The index test was the ANS and the reference standard was the CTN swab. In the laboratory, all samples were eluted into 500ul phosphate buffered saline (PBS). ANS samples were vortexed and pulse spun while CTN samples were swirled. All samples were extracted on Roche MagNA Pure 96 system using MagNA Pure 96 DNA and Viral NA Small Volume Kit and tested on the AusDiagnostics Respiratory Pathogens 16-well assay (Mascot, Australia), on the AusDiagnostics High-Plex 24 system. The respiratory panel included SARS-CoV-2 (with SARS-CoV-2 ORF-1 and ORF-8 genes), influenza A, influenza B, respiratory syncytial virus A and B, rhinovirus, enterovirus, parechovirus, parainfluenza 1–4, adenovirus, human metapneumovirus and two bacteria, 