Performance validation by simulated ST data
melanoma
MELANOMA
To evaluate the performance of SpaCET, we deconvoluted the simulated (this section) and real ST data with double-blind histopathology annotations (next section). The simulated ST dataset was generated by mixing 3–10 single-cell transcriptomic profiles from one scRNA-seq dataset to imitate the signal of a spot. Thus, the actual fraction of cell types at each synthetic ST spot was known. As evaluation metrics, we calculated Pearson correlation (We collected 10 scRNA-seq datasets from melanoma