Ethics approval and consent to participate
All methods were carried out in accordance with relevant guidelines and regulations Declarations of Helsinki. Informed consent was obtained from all subjects and/or their legal guardian(s). All study participants provided informed consent to participate in accordance with the protocol on bioethical examination No. 158 of may 23, 2022, issued by the Commission on Biotic Expertise and Research Ethics of the Bogomolets National Medical University, Ministry of Health of Ukraine.The study protocol was approved for research by the Commission on Biotic Expertise and Research Ethics of the Bogomolets National Medical University, Ministry of Health of Ukraine.