Background
congenital disabilities
PREGNANCY LOSS
With the postponement of women’s marriage and childbearing in recent decades, the number of women with advanced maternal age (AMA) seeking in vitro fertilization (IVF) has increased significantly. However, owing to the reduction of euploid embryos in women with AMA, they experience a higher prevalence of IVF failure, pregnancy loss, and congenital disabilities in offspring compared to younger women [The growth hormone (GH) is a polypeptide hormone secreted by the anterior pituitary gland, which plays a role in promoting cell division and growth by directly binding to receptors on target cells or stimulating the liver to secrete insulin-like growth factor (IGF) [In recent years, studies have shown that supplementation with recombinant human GH (rh-GH) during OS may improve the ovarian response and clinical outcomes of IVF. A study of preovulatory ovarian follicles and follicular fluid showed that GH could upregulate the expression of follicular-stimulating hormone (FSH) and luteinizing hormone (LH) receptors in granulosa cells in women aged 39–45 years, thereby improving ovarian response and pregnancy outcomes in this population [Obtaining euploid embryos is a prerequisite for healthy live births and is considered the primary goal for OS in women with AMA. It is important to evaluate the effect of GH supplementation on the ploidy status of blastocysts in this population and therefore explain the underlying reason for the effect of GH on IVF outcomes. Thus, the present study aimed to compare the euploidy status of blastocysts obtained from patients with AMA with or without GH supplementation during OS.