Statistical Analysis
REGRESSION
All statistical analysis was performed in R (version 4.0.1). Comparison of Samples where optical density measured by ELISA fell below the detection limit of the standard curve, were allocated a random value between zero and the limit of detection. Antibody titers in Luminex data were interpolated using xPonent 4.2 software (Luminex Corporation) using 5-parameter logistic regression. For the 4-plex Luminex assay, where both limit of accurate quantification and detection were characterized, results falling below respective limits were randomly assigned a value between zero and the lowest value of each limit [