2.3.2. Analytical Methods 
Fasting venous blood
Fasting venous blood samples were collected in the morning (08.00–09.00 h). After centrifugation, the serum was separated and frozen at −80 °C. Protein S100B was measured through the sandwich enzyme-linked immunosorbent assay (Sandwich ELISA), which uses a capture antibody and a detection antibody conjugated with isoluminol. The equipment was provided by LIAISON