Discussion
postprandial skeletal muscle
This study investigated the 1-year impact of supplementation with proteins alone or in combination with resistance exercise on basal overnight fasting and 4-h postprandial skeletal muscle protein synthesis rate and muscle metabolome profile.No impact of the protein supplementation arms was found on the muscle protein synthesis rate. Similarly, 1-year exercise with protein supplementation did not seem to affect the muscle protein synthesis rate in comparison to protein supplementation alone. Further, this study confirms the possibility of measuring the metabolome using a low amount of muscle tissue sample (~ 25 mg) using an untargeted GC–MS based metabolomics approach.With respect to the 1-year intervention arms, we did not find any effect on the FSR responses, which was in contrary to our hypothesis and despite that participants in both COLL and WHEY significantly increased their protein intake [As for the basal muscle FSR, we did not find any sex differences: unpaired Acknowledging the sparse data on sex differences on the postprandial responsiveness to protein intake, we performed a Human muscle metabolome data have to our knowledge only been presented in three previous studies [