Sampling
BLOOD, COLD
Study samples were collected during VMMC visit 5 h or 21 h ± 40 min after the last PrEP dosing. Blood and foreskin tissue, including inner and outer, were collected. VMMC was performed using the dorsal slit method according to local guidelines. Tissue was placed immediately in cold culture media DMEM. Samples were immediately transported to the laboratory on ice (median transit time 30 min). Processing of samples was performed immediately upon arrival to the laboratory.