Determination of VCN by droplet digital PCR
BLOOD
Genomic DNA was extracted with the QIAamp DNA Blood Mini or Micro Kit (QIAGEN), and whole-genome amplification was performed with the Repli-g Mini Kit (QIAGEN) on DNA from FACS-sorted HSPC subpopulations, according to the manufacturer’s specifications To evaluate the number of lentiviral vector copies integrated per genome, the droplet digital (ddPCR) technique was used as previously described