Statistics and software analysis
Immunological data was presented as median with 95% CI following non-normal distribution, and nonparametric tests were applied. For determining differences between groups, the Mann-Whitney test was used, and Wilcoxon test was applied for differences within groups. A probability level of < 0.05 was considered statistically significant. Calculations were performed using GraphPad Prism 8.0.1 for Windows (GraphPad Software, La Jolla, CA, USA).Clustering heatmap of immune cytokine secretion data were constructed using ClustVis software (Fast and stimulated C-peptide values were calculated during the clinical trial (