PD analysis
PD
BLOOD
Blood samples for PD analysis were collected at predose (0), 2, 4, 6, 8, 10, 12, and 16 h on day 1, predose on day 2, day 3, day 7, day 11, and day 13, and predose, 2, 4, 6, 8, 10, 12, 16, 24, 36, and 48 h on day 14. The testosterone levels were detected using the chemiluminescence method with the Sciex Triple Quad 6500 + by Frontage Laboratories Co. Ltd. (Shanghai, China). The lower limit of quantification (LLOQ) for testosterone was 0.05 ng/mL. The levels of FSH and LH were measured using the Architect iSR2000 Immunoassay analyzer (the LLOQ for FSH and LH was 0.75 and 0.11 mIU/mL, respectively) and Alinity analyzers (the LLOQ for FSH and LH was 0.05 and 0.09 mIU/mL, respectively; Abbott Laboratories, Abbott Park, IL, USA) by KingMed Diagnostics Group Co., Ltd. (Guangzhou, China).