Tumor mutational Burden and Next-generation Sequencing Analysis
tumor
TUMOR, DNA DAMAGE
Genomic analysis of germline and tumor DNA assessed loss-of-function variants in a number of DNA damage response (DDR) pathways (homologous recombination/non-homologous end-joining, nucleotide excision repair, mismatch repair, and BER) genes by next-generation sequencing (NGS). Nonsynonymous somatic tumor mutational burden (TMB) was measured. High TMB was defined as > 100 mut/Mb in OncoDNA assay. OncoDNA sequencing panel was used for NGS analysis of 311 genes commonly involved in HNSCC including homologous recombination deoxyribonucleic acid repair (HRD) genes. Genomic data were obtained from OncoDNA (