The influence of sequencing depth on gene representation of the PBMC transcriptome
We next assessed the influence of sequencing depth on gene representation in our datasets. To establish a comprehensive reference of genes expressed in PBMCs, we first pooled, The impact of sequencing depth on gene representation by establishment of a deep “compendium” transcriptome of PBMCs. The x-axis represents simulated datasets (n=50) with coverage x, the y-axis shows the percentage of genes that we considered truly expressed in the PBMC compartment for genes that met the filtering cutoff. Bar plots summarizing the mean percentage of the detected PBMC compartment genes by coverage and read filter criterion.