2.6. Statistical analysis
The analysis was conducted using the statistical program GraphPad Prism 9 (GraphPad Software, Inc., La Jolla, CA, USA). The mean and standard deviations were used to present the data. The analyzed outcomes complied with the normality of data through the Shapiro–Wilk test. Subsequently, a two-factor mixed analysis of variance (ANOVA) model with repeated measures was employed to measure the time × group effect of all the variables. A Bonferroni multiple comparison test (