2.3. Study Design
Each participant completed four 300-min trials (randomized, investigator-blinded, crossover design), with each trial separated by a minimum of three days. Participant treatment order randomization was performed by a clinical laboratory manager, who did not participate in data analysis or interpretation, using online software (Randomization.com). While the participants, clinical laboratory manager, and dietitians were not blinded, the investigators were blinded until all participants finished the protocol and all sample analyses were completed. Before each testing day, participants were asked to refrain from performing physical activity for 48 h. Participants were provided a controlled meal to consume to satiation the evening before each testing day (1052 kcal: fat: 25.5 g; carbohydrate: 163.6 g; protein: 47.2 g) (