Measurements and assays
FAI, acuson, Hypertension
HYPERTENSION
At the baseline visit, included participants underwent a physical examination and height, as well as systolic and diastolic blood pressure were measured. Hypertension was defined as systolic blood pressure ≥ 140 mmHg, diastolic blood pressure ≥ 90 mmHg and/or using anti-hypertensive drugsTrans-abdominal ultrasonography was performed by experienced radiologists. Ovarian volume and a total number of antral follicles (2–9 mm in diameter) were measured using a 3 to 5.5 MHz curvilinear probe (acuson s2000, Siemens Medical Solutions, USA).Blood samples were collected after overnight fasting and stored at − 20 °C until the analysis. Sex hormone binding globulin (SHBG) was determined by enzyme-linked immunosorbent assay (ELISA) kits (Demeditec, Germany) with the inter-assay coefficient of variation (CV) of 5.2%. The free androgen index (FAI) was determined by the equation FAI = total testosterone (ng/mL) × 3.47/SHBG (nmol/L)