Flow cytometry
To observe the level of ILC2 in subjects, RPMI- 1640, which contained streptomycin, 5% human AB serum, penicillin, and glutamine (5 mmol/L) solution, was added to 24-well plates, and the isolated PBMCs were cultured at a concentration of 2 × 10culture for 3 h. The lineage cocktail method was used to sort ILC2s according to a method described elsewhere [