2.6.2. Habitual Coffee Intake Study
Molecular genetic analysis was performed with DNA samples obtained from leukocytes (venous blood). Four ml of venous blood were collected in tubes containing EDTA (Vacuette EDTA tubes, Greiner Bio-One, Kremsmünster, Austria). DNA extraction and purification were performed using a commercial kit according to the manufacturer’s instructions (Technoclon, Moscow, Russia). HumanOmniExpressBeadChips (Illumina Inc., San Diego, CA, USA) or HumanOmni1-Quad BeadChips (Illumina, San Diego, CA, USA) were used to genotype 