Statistics and reproducibility
tumor
DISEASE PROGRESSION, TUMOR
No statistical method was used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment. Eight samples were removed due to failed library preparation or sequencing quality control. For TMB analysis, one sample was removed with the TMB score annotated as ‘not detected’. For TCR-sequencing analysis, one sample was removed due to bad quality. Owing to limited biospecimen availability, IHC staining was performed only once for each tumor sample. All analyses were performed and plotted on R.4.0 in Jupyter notebooks. For survival analyses, as patients received atezolizumab until loss of clinical activity or disease progression