Flow Cytometry
In all flow cytometry experiments using primary cells, nonspecific binding was blocked with human or mouse Fc block (Miltenyi Biotec) on ice for 30 minutes prior to incubation with detection antibodies. Intracellular staining was conducted using the Transcription Factor Buffer set (BD Biosciences), per manufacturer's instructions. Following fluorescence compensation, data were acquired on FACS Canto II or Fortessa (BD Biosciences) and analyzed with FACSDiva (BD Biosciences, RRID:SCR_001456) or Flowjo (BD Biosciences, RRID:SCR_008520) software.