Anthropometric measurements and blood chemistry
postprandial venous blood, adiposity
ADIPOSITY
Measurements were recorded for waist circumference using a steel tape around mid-point of lowest point of last rib and upper part of pelvic bone. Body composition using DEXA (Prodigy™, Lunar Corporation USA) was measured twice, at the start and end of trial and used for total weight and reporting regional adiposity measurements. Body weight on a calibrated scale and multi frequency Bioimpedence (BIA) Analysis (In Body 3.0, Biospace, Seoul, South Korea) was used each fortnight to track compliance to energy restriction during the trial. Fasting and 2 h (OGTT- 75 g glucose) postprandial venous blood was collected by a trained phlebotomist at the start of the experiment and at the end of 8 weeks. Centrifuged and aliquots of blood was stored at −80 