2.6. Assessment of Inflammation Markers
TNF-α
The assessment of IL-6 was made by using an R and D Human IL-6 Quantikine HS ELISA Kit (HS600C, R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions. The measurement of TNF-α was made by using a Biorbyt Human TNF alpha ELISA Kit (orb50111, Biorbyt Ltd., Cambridge, United Kingdom) according to the manufacturer’s instructions. All of the samples were analyzed in a Thermo Scientific Varioskan LUX multimode microplate reader (Thermofisher Scientific, Vartaa, Finland).