Preclinical evaluation of IT anti-PD1
The Institutional Animal Care and Use Committees of MD Anderson approved all mouse experiments. Female only C57BL/6 mice between 8 and 10 weeks old were purchased from the Jackson Laboratory. Experiments using these mice were performed at the MD Anderson South Campus Animal Vivarium. The Department of Veterinary Medicine and Surgery provided veterinary care, including routine husbandry and health monitoring, facilities and services in support of the Institutional Animal Care and Use Program, in keeping with all applicable laws, regulations, guidelines and the Association for Assessment and Accreditation of Laboratory Animal Care accreditation standards. This facility is a dedicated pathogen-free rodent barrier with quarantine and hazard containment provided as well. Mice were kept in HEPA-filtered laminar flow racks in a class 100 cleanroom. Mice were given unlimited access to standard mouse chow and water. Animals are housed in micro-isolators provided by the animal care facility. All animal facilities at MD Anderson operate in compliance with National Institutes of Health regulations as outlined in the DHHS Guide for the Care and Use of Laboratory Animals, the Animal Welfare Act and other relevant Federal Laws. Isotype rat IgG control antibody (BioXCell, clone 2A3, catalog no. BE0089) and anti-mouse PD1 antibody (BioXCell, clone RMP1-14, catalog no. BE0146) were purchased to evaluate the safety of IT anti-PD1 administration.IT treatments were performed via direct injection into the cisterna magna