In vitro toxicity
death
DNA DAMAGE
Two cell lines; the alveolar type II-like cell line (A549) and the bronchial epithelial cell line (BEAS-2B) were exposed to filter-collected and extracted wood smoke particles (WSP) at four different concentrations. A significant decrease in metabolic activity, reflected by MTT-assay, was found in BEAS-2B cells at the 75 µg/mL WSP concentration (Fig. In vitro data showing; DNA damage was assessed with Comet assay and expressed as OTM (Fig. Apoptotic cell death was measured through quantification of Annexin V binding to externalised phosphatidylserine. Although there was an increase in Annexin V positive BEAS-2B cells at the highest dose of WSP, this was not statistically significant (Cell cycle phase distribution was assessed in cells after exposure to WSP. In A549 cells, a significant increase in cells in G0 phase was found at the highest concentration, 300 µg/mL. In BEAS-2B cells, a significant increase in cells in the G0 phase was also seen, as well as a decrease in cells in S-G2-M phase at 300 µg/mL (Fig. In vitro cell cycle analysis in two different cell lines; 