Histological characterization
tumor
TUMOR
The second half of the biopsies of mCRC uncultured and TDOs (10 to 18 days) were fixed and histologically evaluated using Tissue-Teck VIP 6AI Tissue Processor (Sakura Finetek, Japan). Both samples were stained with hematoxylin-eosin to confirm the presence of tumor cells. The CDX2 (clone AMT 28, NovoCastra; 1:50) expression was evaluated using the Benchmark Ultra automated instrument (Ventana Medical Systems, Roche, Tucson, USA).