Mean normalized ELISA optical density over time.
cryptosporidiosis
CRYPTOSPORIDIOSIS
Normalized OD values from days -1, 1, 2, 4, 6, 19–24, and 41–55 were averaged according to participant randomization to the CFZ or placebo arms of the trial. Circle markers—participants randomized to CFZ; square markers—participants randomized to placebo.In summary, this study demonstrates that ELISA and qPCR can demonstrate differences between groups under study for cryptosporidium. The limitation of the ELISA is the numbers of subjects are much less than can be detected with qPCR. However, the relative ease of ELISA may outweigh the need for enrolling high numbers of subjects in certain groups where cryptosporidiosis is of high prevalence.