Liquid chromatography with tandem mass spectrometry analysis
Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) analysis was performed using quadrupole Orbitrap mass spectrometers, Q-exactive plus (Thermo Fisher Scientific, Waltham, MA, USA), coupled to an Ultimate 3000 RSLC system (Dionex, Sunnyvale, CA, USA) with a nano-electrospray source as previously described, with some modifications [