2.3. Clinical and Biochemistry Analyses
fasting insulin (µU/mL)/22.5.Cortisol, TG
BLOOD, INSULIN RESISTANCE
Study visits were scheduled at screening, baseline, and after two weeks. Body weight and height were measured. Body mass index (BMI) (kg/mAlthough the exact indoor temperature and brightness of the hospital were not measured, it is estimated that the optimal indoor temperature within the hospital would have been maintained between 18 and 20 °C. All participants visited the hospital before 8 a.m. for their blood tests. Blood samples were obtained after more than 8 h of fasting. White blood cell (WBC) counts were quantified by flow cytometry using XN2000 (Sysmex, Kobe, Japan). Insulin levels were analyzed by the chemiluminescent microparticle immunoassay using the Architect i2000SR (Abbott, Abbott Park, IL, USA). Lipids (total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), non-high-density lipoprotein cholesterol (non-HDL-C), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C)) were analyzed using an enzymatic color test. C-reactive protein (CRP) levels were analyzed using an immunoturbidimetric method. The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated using the following equation: fasting glucose (mmol/L) × fasting insulin (µU/mL)/22.5.Cortisol and adrenocorticotropic hormone (ACTH) levels were assessed using an electrochemiluminescence immunoassay (Roche Cobas 8000 e801, Mannheim, Germany). Serotonin levels were assessed by liquid chromatography-MS/MS using an electrochemiluminescence immunoassay (SCIEX, 5500 Qtrap, Framingham, MA, USA).A heart rate variability (HRV) analyzer with a three-lead electrocardiogram (Medicore Co., Ltd., Gyeonggi, Republic of Korea) was used for 5 min in the supine position in a quiet room. Common HRV indices, such as the standard deviation of normal-to-normal intervals (SDNN), total power (TP), low frequency (LF), high frequency (HF), and the LF/HF ratio, were measured.