Statistical analysis
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Data distribution was evaluated using the Kolmogorov–Smirnov test. The normal distribution data for the one-way ANOVA test with Tukey post-test was used. For other data, the Kruskal–Wallis test with the Dunn post-test was used. For frequency comparisons, the Chi-square test was used. The analyses were performed using the GraphPad Instat® program (Version 3.06, 2003). Data were presented as mean ± standard error of the mean or mean ± standard deviation, and the established significance level was 