Oxidative stress
OXIDATIVE STRESS
Oxidative stress was assessed in vitro through 2′, 7′-dichlorodihydrofluorescein diacetate (DCF) assay, which measures intracellular ROS activity. Cell suspensions were analysed on a 96-well plate. DCF stock solution (5 mM DCF reagent (Sigma) dissolved in DMSO) was added diluted 1:10 with PBS. The plate was incubated for 30 min at 37 °C before fluorescence was measured (excitation 485 nm, emission 530 nm). Fold change of ROS activity was calculated using the blank control.