Cytokine measurements
peripheral inflammation, WBH, tumor necrosis
TUMOR NECROSIS
To evaluate the effect of WBH on peripheral inflammation, we used a high-sensitivity 9-plex multiplexed ELISA (Cat. No. 85-0217, Aushon Biosciences, Billerica, MA, USA; now provided as a 10-plex, Cat. No. 85-0002 by Quanterix, Lexington, MA, USA). Use of the multiplex allowed for an examination of a range of circulating cytokines, including interferon (IFN)-gamma, interleukin (IL) 1-alpha, IL-1-beta, IL-4, IL-6, IL-8, IL-10, IL-12p70 (heterodimer), and tumor necrosis factor (TNF; active trimer). Assays were conducted using duplicate samples according to manufacturer’s instructions, and concentrations were measured using the Aushon Signature Plus