Laboratory procedures
infection
HEPATITIS B, HEPATITIS B, INFECTION
HIV testing was performed on serum using two or more rapid antibody diagnostic tests administered serially (Entebbe and Kampala) as follows: Determine screening test (Alere DetermineAll sites measured hepatitis B surface antigen (HBsAg) and total hepatitis B core antibody (HBcAb (Total)). The site in Kampala also measured IgM antibodies to core protein (HBcAb (IgM)) while all sites measured antibodies to surface protein (anti-HBs) in at least a subset of volunteers who were also screened for an immunology sub-study. This sub-study aimed to assess how the pre-existing immune status affected responses to Hepatitis B vaccination and therefore needed individuals who had never been exposed to Hepatitis B either through vaccination or prior infection. The assays used to test serum samples for Hepatitis B at the 3 sites are shown below (