The Cancer Genome Atlas and Single-cell RNA Sequencing Analysis
NSCLC, melanoma, head and neck squamous cell carcinoma, TCGA, HNSCC, Cancer
DISEASE, MELANOMA, NSCLC, CANCER
Public gene expression data from The Cancer Genome Atlas (TCGA) was used to evaluate ICOS expression on individual cell subsets was evaluated using publicly available RNA sequencing (RNA-seq) datasets from melanoma, head and neck squamous cell carcinoma (HNSCC), and NSCLC (GSE72056, GSE103322, and GSE99254, respectively; refs. Correlative analysis of ICOS expression and overall survival (OS) rates was extracted from publicly available TCGA and metadata of patients with HNSCC. Data were retrieved from TCGA using an internal copy from OmicSoft (accessed January 23, 2020). Data were from a heterogenous patient population including patients with stage I–IV disease. Groups were determined by top (ICOS