3.7. The expression of the miRNAs
PRIMARY TUMOR, METASTASIS, METASTATIC TUMOR
As the 5 miRNAs in risk score were related with the CRC prognosis, we suspected that the miRNAs were related to CRC metastasis. Therefore, the expression of the miRNAs was explored between SW480 and SW620, which were respectively separated from primary tumor and metastatic tumor of CRC. The results showed that miR-328-3p, miR-3934-5p, miR-664b-5p, miR-200c-5p and miR-3677-3p were down-regulated in SW620 compared with SW480, in contrast, miR-200c-5p was up-regulated in SW620 (Fig. The expression of the 5 miRNAs in risk score was compared by qRT-PCR between SW480 and SW620. (A) miR-328-3p. (B) miR-3934-5p. (C) miR-664b-5p. (D) miR-200c-5p. (E) miR-3677-3p.