2.3. Collection and Storage of Donor Human Milk
DM was collected in a special container and was processed by the “Elena Venizelou” Milk Bank using the Holder Pasteurization method (human milk was heated to 62.5 °C for 30 min) and quickly cooled down to 25 °C within 10 min in an ice water bath; it was then stored in a refrigerator at a temperature of 0–4 °C for one to two days or frozen at −20 °C for three to six months. Single donor expressed human milk was collected and pasteurized separately.