HER2 spatial distribution analysis by machine learning
Slides stained for HER2 expression collected at baseline from cohort 1 were digitalized and analyzed through an unsupervised clustering algorithm (The clusters were further analyzed on the ground of nuclei statistics toward the design of interpretable markers using an unsupervised nuclei segmentation algorithmThe association between the confirmed objective response and clusters was assessed through statistical analysis based on each cluster’s relative percentage in each slide. We used a Mann–Whitney 