Statistical analysis
Statistical analysis was performed using SPSS software (version 22; Inc, Chicago, IL, USA). The parameters were compared by use of an analysis of variance (ANOVA) two-way test, followed by Post hoc Tukey simultaneous tests to analyze continuous variables. P_value less than 0.05 was considered statistically significant. The data were analyzed using the two-tailed Student’s t test for independent data, Fisher’s exact test, and two-by-two table between groups where appropriate.