Organoid generation, culture and treatment
tumor, colorectal tumor, Tumor
TUMOR, TUMOR
Patient-derived organoid generation was attempted from baseline biopsies of all patients. A total of 10 colorectal tumor organoid lines were successfully generated from tumor baseline biopsies of 10 patients enrolled in the BRAF/MEK/PD-1 inhibition trial (patients 1, 2, 4, 10, 11, 14, 16, 18, 21 and 24). Tumor biopsies were transported in ice-cold RPMI with 10% human serum and transferred into a petri dish on ice before processing. Tumor biopsies were minced and subjected to enzymatic dissociation in 4.75 ml minimum essential medium for suspension cultures (Gibco) supplemented with 250 µl Liberase for 45 min at 37 °C using a heater-shaker. Following the dissociation, tumor biopsies were centrifuged at 300