Animals
All experimental protocols were approved by the University of California Davis Institutional Animal Care and Use Committee (IACUC). Research was conducted in accordance with all relevant guidelines and regulations of the University of California, Davis, IACUC (Protocol number 20565) and all methods are reported in accordance with ARRIVE guidelines. A sample size of at least 7 cats in each dose group was calculated to have an 80% power to identify a 15% reduction in key parameters of echocardiographically-assessed systolic function. This a-priori sample size was estimated based upon colony-specific, preliminary data in HCM-affected feline subjects