2.4. Study Participants and Schedule
Healthy adults aged 30 to 60 years (Subjects assigned to the caviar, combination, or placebo groups consumed the sample or placebo supplement daily for eight weeks. At 0, 4, and 8 weeks, measurements of skin hydration, wrinkles, and elasticity were conducted. Unless otherwise noted, each subject washed their face, waited for 30 min under constant temperature and humidity (22–24 °C, 45–55%), and limited water intake for 1 h before skin analysis. Measurements were taken at the right-angle intersection between the corner of the eye and the tip of the nose, and the average of three measurements was calculated.