Outcome assessments
tumor necrosis, TNF-α
TUMOR NECROSIS
Primary efficacy variable was the change in HbAWe also measured the changes in biomarkers C-reactive protein (CRP), adiponectin, tumor necrosis factorα (TNF-α), fibroblast growth factor-21 (FGF-21), as the exploratory assessments. CRP was determined by latex enhanced immunoturbidimetric assay (ADVIA 1800, ADVIA Chemistry XPT, Dimension EXL, SIEMENS). Adiponectin and TNF-α were measured by enzyme-linked immunosorbent assay (R&D Systems). FGF-21 was quantified by using enzyme-linked immunosorbent assay kits according to manufacturer’s instructions (Duoset human FGF21 ELISA kit, R&D systems, Inc., Minneapolis, MN, USA).