Patients and biochemical analyses
T2DM
BLOOD, SEPARATION
This research included 80 T2DM patients (31 men and 49 women) randomly assigned in a 1:1 ratio to one of two groups: the control group (glimepiride + placebo) or the omega-3 group (omega-3 fatty acids + glimepiride). Since the randomization was done by a study-independent individual using a computerized random number technique in Microsoft Excel, the patients would be unaware of the group to which they have been allocated. At the beginning of the study, the weight and height of all patients were taken using a measuring scale, and body mass index (BMI) was calculated using the following formula: weight (in kilograms)/(height × height) (in meters) [Blood samples were collected in the morning, after an overnight fast, and centrifuged at 3,000 rpm for 10 min at room temperature for serum separation. The fasting serum samples were stored at –80 °C until analysis.The enzymatic colorimetric approach was utilized for measuring serum triglycerides [The patients' adherence was evaluated using a pill count and was calculated by subtracting the number of pills left in the bottle from the total number of pills supplied, during the patient-scheduled refill. The predicted number of pills consumed by participants was determined by multiplying the daily dose by the number of days since the pills were supplied. The number of pills consumed was then divided by the total number of pills at the start, and the result was multiplied by 100 to calculate the percent of pill count adherence [