Pre-study
For 4 d prior to the study day visit, participants were instructed to: follow two repetitions of a standardised 2-d menu based on a self-completed 3-d food record that had been adjusted to provide a comparable percentage energy from macronutrients and relative fibre content to the NGTF (based on the mean content of the two feeds); refrain from strenuous physical activity; and not consume alcohol and caffeine. For the 3 d prior to the study visit, participants were instructed to collect the first stool of the day (using the kit provided) and for every stool to complete the King’s Stool Chart