BMP9 methods
Total soluble BMP9 concentrations in human serum were measured using an enzyme-linked immunosorbent assay, which uses a mouse anti-human BMP9 monoclonal antibody as the capture reagent and recombinant BMP9 as the standard. Captured soluble BMP9 is detected using a biotinylated goat anti-human BMP9 polyclonal antibody followed by streptavidin conjugated with horseradish peroxidase. The lower limit of quantitation of the assay is 31.3 pg ml