Clinical and laboratory procedures
viremia
ANTIBODY-DEPENDENT ENHANCEMENT, VIREMIA
Research blood collections will include serum at all study days, which will be used to assess viremia (days 3–15), cytokine signaling, antibody activity by GMTs and ELISAs, and antibody-dependent enhancement using enhancement assays with cell lines and primary monocytes [All LN FNA procedures will consist of 5 needle aspirations (passes) using a 25G bevel needle with a 10 mL syringe primed with 1 mL of air. Each pass will consist of 10 to 20 s of excursions using light suction. Each needle will be rinsed 2–3 times with 1 mL of media into one 15 mL collection tube, after which cells will be frozen and stored at -140ºC. All LN FNA will ideally be performed from the same lymph node via referencing or registration with local landmarks.Through single cell immune profiling, paired gene and surface protein expression profiles (CITE-seq: Cellular Indexing of Transcriptomes and Epitopes by sequencing [