2.7. RNA Isolation and Reverse Transcription
human skeletal muscle
Total RNA was extracted from frozen skeletal muscle samples utilizing a TRIzol-based method according to the manufacturer’s instructions and as previously described [Real-time polymerase chain reaction (PCR) analysis of human skeletal muscle.A panel of mRNA sequences was selected to investigate the effect of To quantify mRNA expression in human skeletal muscle, real-time PCR was conducted using SsoAdvanced