NK cell product characterization
After expansion on culture day 20, cells were harvested and PB-NK cell purity was evaluated by flow cytometry (CD3To evaluate the functional status of the PB-NK cells using intracellular flow cytometry [interferon (IFN)-γ], CD107a degranulation (all antibodies were purchased from Biolegend-USA), and lactate dehydrogenase (LDH) release assay (Sigma-Germany) against K562 (IBRC-Iran)) as described previously [