Serum proteomic analysis
Serum samples were stored at −70°C until analysis. High-abundance proteins and their protein complexes were affinity-purified from the samples, and the samples were divided into two pools: high- and low-abundance proteins. Human serum albumin was further removed from the high-abundance samples. Proteins were digested with trypsin and applied via a reverse-phase nano-liquid chromatograph column to a high-resolution mass spectrometer. Data were collected in a data-independent manner, and peptides were identified and quantified.