Tissue sampling, PDO generation and drug treatment
PDO
COLD
During surgery, cold cup biopsies will be harvested, digested to single cells and plated organoids are allowed to form. Thereafter, PDO are dissociated to single cells, plated and formed after 48 h. These PDO are then exposed to the compounds in the following conditions: untreated (medium) or treated (0.1% DMSO vehicle control, and 4 drugs that are currently used for intravesical instillation (Epirubicin, Mitomycin C, Gemcitabine and Docetaxel). Compound effect is then measured after 48 h by the CellTiterGlo 3D reagent from Promega measuring luminescense. For intravesical instillation, only these four drugs will be used. However, in-vitro testing will eventually include other compounds, dependent on the number of PDO available.