2.7.2. Libraries Preparation and Shotgun Metagenomic Sequencing
Fragmentation of the extracted total DNA was performed using the FS DNA Library Prep Set kit (MGI Tech, Shenzhen, China). After ligation of adapters to each sample, the libraries generated were purified on magnetic beads. Library size was verified by capillary electrophoresis on at least 10% of samples. After quantification by fluorimetry, the libraries were normalized and pooled before denaturation, single-strand circularization, and sequencing using the DNBSEQ-G400 platform (MGI Tech).