Study setting
This clinical and microbiological comparative study took place at the Department of Prosthodontics, Faculty of Dentistry, and the Department of Microbiology, Medical Research Institute, Alexandria University. This trial has been registered at ClinicalTrials.gov with identification number: NCT05584865, registration date: (18/10/2022). The authors certify that this trial has received ethical approval from the Research Ethics Committee, Faculty of Dentistry, Alexandria University, Egypt (international No.: IORG0008839, ethics committee number: 0441–6/2022; date of registration: 9/6/2022). Signed written informed consent forms were obtained before enrollment in this trial.