Energy expenditure and substrate oxidation rate during submaximal exercise
As described previously, all the volunteers were randomly split into two groups: 10 adolescents participated in COMB training and 11 adolescents participated in a MICT program. To ensure full recovery after the GRAD test, submaximal testing took place two days after the GRAD test. All the participants arrived at the laboratory after a 12-h overnight fast. COMB and MICT were designed to have equal amounts of energy expended. Both COMB and MICT exercises were performed on a motorized treadmill (TechnoGym, Gambettola, Italy). The COMB training test included a 10-min rest period in a standing position on a treadmill, followed by ~ 36 min of walking with 3 repetitions of 2 min at a high intensity (95% of V′ODuring the submaximal tests, V′O