2.7. Measurement of S. aureus Colony
STERILE
The AD lesions were smeared using a sterile swab that had been rinsed with phosphate-buffered saline (PBS) three times and isolated with a pour-plate method no later than four hours. The isolate was cultured in the test tubes filled with Mannitol Salt Agar (MSA) media (Becton Dickinson GmbH, Heidelberg, Germany), which had been sterilized with an autoclave at 121 °C for 15 min. Each test tube was labelled based on each dilution (10