Preparation of BMAC (Fig. 
STERILE, CREST
Before the graft surgery, the bone marrow aspirated from the posterior superior iliac crest by trochar that was inserted 2 cm laterocaudally. Ten millimeters of bone marrow was extracted into sterile plain tubes using a 20-ml heparinized syringe (1 ml of 5000 U/ ml heparin). The centrifugation was performed immediately consistent with the following protocol: 1BMAC aspiration