4.3. Cell Stimulation
To induce inflammatory stimulation, we seeded HaCaT and Hs27 cells in 6-well plates and cultured them for 24 h to reach 50% confluency. The culture medium was removed and replaced with serum-free DMEM supplemented with LPS or 10 μg/mL poly(I:C) and 20 ng/mL TNFα. Cells were incubated with LPS for 24 h. After washing with PBS twice, they were treated with SPM or vehicle (distilled water in serum-free DMEM) for another 24 h. Cells were incubated together with poly(I:C)/TNFα cocktail and SPM for 24 h.