FMT capsule preparation
STERILE
Fresh stool samples from donors were collected in sterile plastic containers. Fresh fecal samples (25%) were mixed with saline (60%) and pharmaceutical-grade glycerol (15%) and filtered using an automatic extraction instrument (TG-01, Treatgut Corporation, China). Microbial suspensions were further centrifuged to obtain the fecal microbiota precipitates. The precipitates were thoroughly mixed and filled into capsules (DrCaps, 19504907), and the capsules were immediately frozen at −80°C. The mean stool per capsule was 0.9 grams.