2.14. Establishment of an analytical system for the human fecal microbiota by qRT-PCR
RT-qPCR was conducted in a 1-step reaction using the SuperScript IV 1-Step RT-PCR method. The methodology involves the use of Total cells = number of cells determined by standard curve* (total yield/total weight of fecal matter used for extraction).The procedure is detailed in Supplementary Methods, Supplemental Digital Content, 