Isolation of cancer cells from tumor biopsies
agitation, tumor
TUMOR, RESIDUAL TUMOR, BRAIN TUMOR, STERILE, PRIMARY TUMOR
To generate the primary tumor cell cultures, the fresh brain tumor tissue from surgical biopsies was minced using sterile scalpel blades and gently dissociated in a biosafety cabinet using 0.025% trypsin solution at 37°C for 10 min with gentle agitation and intermittent resuspension. Dissociated tumor cells were plated in RPMI with 20% FBS, 1% Penicillin/Streptomycin, Gentamycin Sulfate (complete media) in sterile plastic Petri dishes in the presence of residual tumor tissues and incubated at 37°C humid tissue culture incubator in the presence of 5% CO