DNA sequencing
Before sequencing, the quality of the DNA samples was evaluated using agarose gel electrophoresis and the quantity of the DNA was evaluated using Qubit 2.0 fluorometer quantitation. Genomic DNA was randomly sheared into fragments of approximately 350 base pairs. Fragmented DNA was used for library construction with the NEBNext Ultra Library Prep Kit for Illumina (New England Biolabs). The prepared DNA libraries were evaluated using Qubit 2.0, fluorometer quantitation, and Agilent 2100 Bioanalyzer for fragment size distribution. Quantitative real-time PCR (qPCR) was used to detect the final effective concentration with all samples with ≥ 2 nml library DNA were considered valid for library pooling. The library was sequenced using 2 × 150 bp paired-end sequencing on an Illumina platform.