2.7. Meal Test
MRS
After the MRS measurements on the morning of day 15, a meal test was performed. To this end, a breakfast (20% of daily energy needed) matching the assigned diet was provided to the participants at 8:30 AM (t = 0). Before the meal (t = −15) and at t = 45, t = 75, t = 135, t = 195, and t = 255 min, indirect calorimetry measurements using a ventilated hood system (Omnical, Maastricht Instruments, Maastricht University) were performed. VO2 and VCO2 were used at these timepoints to assess the energy expenditure, respiratory exchange ratio (RER), and fat and carbohydrate oxidation. 