Real-time biofilm growth monitoring
PLAQUE
To select the most suitable transport media for periodontal biofilm samples, the subgingival plaque samples were collected in 2 mL of three different transport media, as indicated above, and homogenized by vortexing for 60 sec. Paper points were discarded, samples were vortexed again for 30 sec and split into three equal aliquots for biofilm growth in real-time either fresh or after 24 and 48 h (t=0, t=24, and t=48) of storage at room temperature. At each time point, the aliquots were centrifuged at 10.000 rpm for 3 minutes, supernatants were discarded, and bacterial pellets were resuspended with 400 µL of BHI supplemented with vitamin KFor biofilm growth evaluation of periodontal plaque samples that were stored at room temperature for 0, 24 and 48h (t=0, t=24, and t=48h), the xCELLigence RTCA system was used as previously described (