Histological assessment
NAFLD, cirrhosis, Brunt, fibrosis
LIVER FIBROSIS, LIVER, CIRRHOSIS, FIBROSIS
Two expert pathologists independently performed the histological assessment of liver biopsies in all study participants. Liver biopsies were processed using neutrally buffered formalin for 24–48 h and were embedded in paraffin. Blocks were cut at 3 μm, and slides were stained among other with hematoxylin and eosin (HE) and picro sirius red (PSR). Liver Fibrosis was staged from 0 (no fibrosis) to 4 (cirrhosis). The NAFLD activity score (NAS) was used to assess biopsies (Brunt et al., 