2.3. Supplement Preparations
MH, T Block
COLD, CROSS
Whole avocado fruit of the Hass variety was obtained from an Australian commercial crop (De Paoli Farms, Queensland, Australia) in an unripe state and stored in a cold room under 5 °C. Within 24 h after the harvest, the whole fruit was washed with water, and the flesh and peel were commercially shredded by a disintegrator, before being transferred into a freeze-drying machine for 96 h. The mass percentage of MH in avocado extract (AvX) was 1.9%, which was determined by HPLC at Southern Cross University (T Block Level 3, Military Road, Lismore, NSW, Australia) according to the methods developed by Kappler-Tanudyaya et al. [