Evaluation parameters
carious bacteria, caries, tooth surface/total tooth, caries, periodontal disease, tooth
ADHESION, PERIODONTAL DISEASE, CARIES, PLAQUE

Assessment of plaque adhesion status using the plaque control record (PCR).Measurements with a saliva analyzer (Sill‐Ha, Arkray Marketing Inc.).Plaque‐disclosing dye (Shofu Inc.) was used to dye all the remaining tooth surfaces, and the state of plaque adhesion on the surface in the cervical region was scored. The method of implementation was prescribed for four tooth surfaces (buccal, mesial, distal, and lingual/palatal) before and after intermaxillary fixation, and for three tooth surfaces (buccal, mesial, and distal) during intermaxillary fixation, and multiple clinicians were assigned without blinding. Records were recorded on a standardized paper chart, and the analysis was calculated as plaque‐positive tooth surface/total tooth surface × 100 and was evaluated by one person in a blinded fashion. The saliva analyzer uses patients' saliva to measure six parameters related to caries (carious bacteria, pH, and acid buffering capacity), periodontal disease (leukocyte count and protein levels), and oral cleanliness (ammonia levels). Specifically, saliva was collected after the mouth had been washed out with 3 mL of water for 10 s. Drops of saliva were placed on a special test paper, which was measured by the analyzer. The ammonia level was measured by the glutamate dehydrogenase assay. Measurements (1) and (2) were made at three time points: immediately before maxillomandibular fixation (baseline); on Day 10 of fixation; and immediately after fixation was released. There was individual variation between patients in the intraoral environment at baseline. Therefore, the amount of change in each measured value over time was calculated and compared. In this study, the primary outcome of this study was focused on PCR to evaluate oral hygiene status. Along with the primary outcome, six parameters related to caries, periodontal disease, and oral cleanliness in saliva were assessed.