4.3. Bioinformatics and Statistical Analysis
Demultiplexed sequences were quality filtered using QIIME (Quantitative Insights into Microbial Ecology) version 2.2020.2 [To remove contaminants that were seen in sequenced controls, we used an in silico approach using the Decontam package in R software (v 4.3.2) [All univariate statistical analysis was carried out in the R software using the Vegan package version 2.5.7 [