Identification of differentially expanded/contracted clones
tumors
TUMORS
ITCs that had a significant increase in frequency after treatment compared with baseline were defined as differentially clonal expanded ITCs, whereas those with a significant decrease in frequency were defined as differentially clonal contracted ITCs. For differential frequency analysis of T clones between baseline and post-treatment tumors, Fisher’s exact test was used to determine differential expanded and contracted clones based on the clonotype count before and after therapy. 