2.5. Statistical Analysis
All continuous data were presented as mean standard error of the mean or median (interquartile range) for parametric or nonparametric continuous variables, respectively. The baseline characteristics between the two groups were compared using a Student’s unpaired The sample size calculation and power analysis were performed as follows: First, we estimated a treatment effect of 1 kg and standard deviation of 5 kg on fat mass and calculated effect size before study. Next, we calculated the sample size using the G*Power 3.1 software (Heinrich-Heine-Universität Düsseldorf) for MAC. A two-way repeated measures ANOVA indicated that a total sample size of 52 was needed to reach 80% power, in order to detect the interaction effect size of 0.20 at a significance level of 0.05.