Peripheral blood mononuclear cells (PBMC) isolation and flow cytometry and data analysis
REA110
STERILE
We acquired blood samples from the recipients at two days before transplantation, one-week post-transplant, and one, three, and six months post-transplant. PBMCs were separated by density-gradient centrifugation using lymphocyte isolation sterile solution Ficoll-PaqueIn flow cytometry analysis, subsets of natural killer (NK) cells were stained with the following antibodies: PE-conjugated anti-CD56 antibody (5.1H11, IgG1κ, BioLegend), FITC-conjugated anti-CD3 antibody (UCHT1, IgG1κ, BioLegend), PerCP/Cy5.5-conjugated anti-CD57 antibody (HNK1, IgM, BioLegend), PE/Cy7-conjugated anti-NKG2D (CD159c) antibody (1D11, IgG1κ, BioLegend), APC-conjugated anti-NKG2A (CD159a) antibody (REA110, IgG1κ, Miltenyi Biotec), and APC/Cy7-conjugated anti-CD16 antibody (B73.1, IgG1κ, BioLegend). Lymphocytes that were CD3