2.4. Sampling
At each intervention visit, a baseline blood sample (9 mL) was collected immediately before administration of the IP. Further blood samples (9 mL each) were collected 0.5, 1, 1.5, 2, 4 and 12 h after the intake. EDTA-plasma samples were obtained and stabilized with 10% V/V of 2 M aqueous citric acid.At each intervention visit, a baseline urine sample was collected from –240 to 0 min before administration of the IP. A further 6 urine samples were collected after the intervention from 0 to 30 min, 30 to 60 min, 60 to 90 min, 90 to 120 min, 120 to 240 min, and 4 h to 12 h. Urine samples were stabilized with 1.88 g/L of ascorbic acid and their volume was measured in order to be able to convert the proportion of excreted metabolites at each measurement point to the excreted volume in a comparable manner.Stabilized plasma and urine samples were stored at −80 °C until analysis.