RNA-seq and analysis
adenomas
ADENOMAS
RNA-seq was performed on functional adenomas. RNA-seq was performed by the Barts and the London Genome Centre, Blizard Institute in London. Briefly, quality control was performed using an Agilent RNA 6000 Nano reagent kit and run on an Agilent 2100 bioanalyzer (Agilent). Only samples with an RNA integrity number number of >0.8 were sequenced. Library preparation using the mRNA library preparation method was performed using a NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). Finally, sequencing was performed on Illumina’s NextSeq 500 system using the high-output 150 cycle kit version 2.5. Partek Flow (Partek) was used for RNA-seq analysis. Sequences were aligned to hg38 with STAR -2.6.1d and annotated genes were aligned to Ensembl Transcripts release 93 with Partek’s own annotation tool. Partek’s GSA tool was used to generate lists of differentially expressed genes.