Fluctuations in circulating innate immune cells after bacterial challenge.
infection
INFECTION
During natural encounter or challenge, the bacterium itself triggers innate immunity at the site of infection (mucosal surfaces); moreover, PT is known to induce systemic effects (Total numbers of circulating leukocytes and neutrophils (expressed as ratio vs. baseline) did not change after bacterial challenge (To investigate whether the kinetics of innate immune cells are associated with colonization, we compared the numbers of circulating innate immune cells in participants who were colonized and participants who were protected against colonization. Similar to the collective kinetics, we assessed the longitudinal changes per cell population per colonization status. In order to avoid presenting an excess of data, only the time points where cell populations significantly differed from each other are shown in this paper. No consistent differences between colonized and colonization-protected participants were observed for leukocytes, neutrophils, eosinophils, iMos, cMos, and DCs. An increase in circulating ncMos (ratio vs. baseline), especially CD36Next, we divided the colonized participants based on colonization density (low- or high-density colonization). Here, we found that the decrease in (especially CD36To summarize, we observed fluctuations in innate cell numbers in the early days after bacterial challenge. Decreased cell numbers may indicate migration of cells into the tissue, and the shedding of CD62L may indicate a shift toward a more activated phenotype in the cMo compartment. Cellular kinetics differed between colonization-protected and colonized participants, and between high- and low-density-colonized participants. The colonization density did not reflect the magnitude of cellular changes, as these were usually more prominent in low-density-colonized participants.