ADCC assays
BLOOD, CYTOTOXICITY, PROLIFERATION
In the ADCC assay, Ba/F3 cells overexpressing EGFR insASV, insSVD and insNPH were labeled with CellTrace Violet Cell Proliferation Kit (Invitrogen, #C34557) before pre-treated with 0.00001, 0.0001, 0.001, 0.01, 0.1, 1, 10, 100 μg/mL JMT101 (JMT-Bio Technology, # DP10720210902) or equivalent dose of Human IgG1, kappa Isotype Control (SinoBiological, #HG1K, R1 clone, lot: MA16MY1804) for 30 min at 37 °C. NK cells were isolated from Peripheral Blood Mononuclear Cells, then co-cultured with three types of target cells at 37 °C (effector: target = 4:1) for 4 h. Cytotoxicity were analyzed by flow cytometry. Dilution methods for antibodies used in ADCC assays are provided in the Source data file.