Procedures
DSM Nutritional Products
ADVERSE EVENTS
MMPs were produced under good manufacturing practice by DSM Nutritional Products (Johannesburg, South Africa). The MMPs used in this study contained 15 micronutrients (Study participants were recruited in cohorts (From day 2 to day 84, all children were seen on a daily basis by a fieldworker who supervised administration of the MMP, and recorded and managed any adverse events. Field workers collected data on morbidity twice a week. At day 7 and weekly thereafter (except on days 49 and 84 when venous blood was collected), capillary blood was sampled for the measurement of haemoglobin by the HemoCue analyser, Plasma hepcidin concentrations were measured weekly by ELISA with a detection range of 0·049–25·000 μg/L (human hepcidin-25 EIA Kit, Peninsula Laboratories International, San Carlos, CA, USA) on the day of blood collection. Results were available on the following day. The assay was later validated as part of the worldwide harmonisation exercise.