2.6. Markers of the Intestinal Barrier Integrity
Tecan, LBP
Fecal zonulin levels were determined by enzyme-linked immunosorbent assay (ELISA) (Immundiagnostik AG, Bensheim, Germany) according to the manufacturer’s protocol. Absorbance was measured with a spectrophotometer (Sunrise, Tecan, Männedor, Switzerland) at 450 nm.The concentration of LPS and occludin in blood serum was determined using an ELISA assay (EIAAB SCIENCE INC, Wuhan, China), and that of LBP was determined using an ELISA assay (FineTest, Wuhan, China) according to the manufacturer’s protocols. In each case, absorbance was measured using a spectrophotometer (Sunrise, Tecan, Männedor, Switzerland) at 450 nm.