Cytokines
toxicity, CRS
BLOOD, CLOT, CYTOKINE RELEASE SYNDROME
Blood samples (10 mL) were drawn for cytokine analysis in 10 mL red-top tubes to evaluate the toxicity risk of cytokine release syndrome (CRS). Blood samples were drawn prior to the first infusion dose on C1D1, 24 h post EOI, 72 h post EOI, 14 days post EOI and prior to C3D1. After drawing, blood was allowed to clot at room temperature for a minimum of 2 h and then red-top tubes were centrifuged for 10 min at the 2000 rpm with full brake.After centrifugation, the serum layer was aliquoted into cryovials designated for immune-monitoring research samples. Cryovials were frozen and stored at -80˚C until assays were performed. Cytokines (IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-α, IFN-γ) were evaluated using the V-PLEX Proinflammatory Panel 1 Human Kit (Meso Scale Discovery, Rockville, MD, USA), according to manufacturer’s instructions.