MMP activity.
AS-72017
MMP2 activity was studied using the MMP2 Biotrack activity assay (RPN2631; GE Healthcare) and the SensoLyte Plus 520 MMP2 assay (AS-72224; AnaSpec). MMP9 activity was studied using the SensoLyte Plus 520 MMP9 assay (AS-72017; AnaSpec). The manufacturer’s instructions were followed in full. Urinary active MMP2 and MMP9 were normalized to creatinine. Glomerular active MMP2 and MMP9 were normalized to total protein using the Pierce BCA protein assay (23225; Thermo Fisher Scientific). The fold change relative to control was calculated to enable pooling of results from different experiments.