Ethics approval and consent to participate
This study was first approved by ethics committee( Iran national committee for ethics in biological research), then with the number IR.SBMU.RETECH.REC.1399.279 and with the code IRCT20200428047229N2 from Shahid Beheshti University of Medical Sciences and Health Services in the trial registration center. Clinical Clinic of Iran (IRCT) was registered. All participants in the study were asked to complete the consent form to enter the study.All methods were carried out in accordance with relevant guidelines and regulations. informed consent was obtained from all subjects and/or their legal guardian(s).