Blood collection
BLOOD, SEPARATION
Blood samples for irisin, myostatin, IL-18, BDNF, CAF, 25-(OH)D3, insulin, and glucose assessment were collected between 7:00 and 9:00 AM after overnight fasting at two time-points: at baseline and after completion of the 12-week intervention. A qualified nurse took venous blood from the antecubital vein into vacutainer tubes (Vacutainer SSTTM II Advance) for serum separations and tubes with EDTA as an anticoagulant for plasma separation. Samples were centrifuged at 2000