Mass cytometry (CyTOF)
Mass cytometry (CyTOF) was performed on PBMCs obtained on cycle 1, day 1 and cycle 3, day 1 of treatment. PBMCs were stained with the CyTOF antibody panel detailed in Supplementary Table Astrolabe was employed for automated computational annotation (Astrolabe Diagnostics). CyTOF analysis was performed using Astrolabe annotated data and statistical modeling with R. The data were loaded into R using the package ‘orloj’. Astrolabe gating strategies were manually reviewed for a subset of samples. Data were uploaded to Cytobank for quality control analysis and visualization.