Sequencing and bioinformatics analysis
ng/µL
STERILE
Fecal samples were collected in sterile plastic cups and stored at -80°C before further processing. Total genomic DNA from samples was extracted using the SDS method. DNA concentration and purity were evaluated on 1% agarose gels. According to the concentration, DNA was diluted to 1 ng/µL using sterile water. Genomic DNA extraction and PCR amplification were performed using fecal samples, and PCR products were pooled and purified. Finally, sequencing libraries were generated using the TruSeq