Pharmacokinetics
OBI-3424 (prodrug) and OBI-2660 (active metabolite) concentrations were analyzed from blood samples collected on day 1 of cycle 1 before, 15 min after the beginning of, and at the end of the infusion and 15, 30, 60, and 90 min and 2, 4, 6, and 8 h after the end of the infusion. Human plasma samples were analyzed by a validated method using liquid chromatography with tandem mass spectrometric detection. Plasma pharmacokinetic parameters were estimated with noncompartmental analysis using Phoenix WinNonlin Software (v8.3).