Bacterial community
nonbacterial
A total of 12,546,211 reads were screened for nonbacterial sequences and reduced to a total of 11,876,729 reads containing 5001 bacterial ASVs. Samples were then screened for contaminants based on the prevalence of bacteria in DNA extraction and sequencing negative control samples using the decontam package (There was no difference in the observed number of ASVs (Comparison of the number of observed ASVs (Panel A) and Shannon Diversity (Panel B) in stool samples from healthy nonelderly adults between pre- and postmixed nut consumption for 4 wk. Principle co-ordinate analysis with Bray–Curtis dissimilarity between pre (circle) and post (square) intervention samples in healthy nonelderly adults receiving placebo (Panel C) and nuts (Panel D), each for 4 wk. ASV, amplicon sequence variant.Comparison of top 10 ASVs showing the greatest difference between groups in the analyses. Pre- and posttreatment data in nonelderly adults who consumed placebo for 4 wk is shown. Wilcoxon analysis revealed no statistical significance between the relative abundance of these populations (Panel A). Comparison of top 10 ASVs showing the greatest difference between groups in the analyses. Pre- and posttreatment data in nonelderly adults who consumed mixed tree nuts for 4 wk. Wilcoxon analysis, False Discovery Rate (FDR) adj. revealed a statistically significance increase in the relative abundance of unclassified 