Human atrial EVs contain antifibrotic/antiinflammatory transcripts and proteins.
POAF, fibrosis
INFLAMMATION, FIBROSIS
Human EDCs were cultured in a clinical cell manufacturing facility from atrial appendage biopsies using serum-free, xenogen-free culture conditions. EVs were isolated from conditioned media after 48 hours in 1% oxygen, basal media conditions (The cargo within EDC EVs was enriched with 83 miRNA transcripts associated with reducing inflammation, stimulating angiogenesis, and suppressing fibrosis (Taken together, these data support the notion that EDCs produce a defined EV product containing an antifibrotic, antiinflammatory cargo with the potential to alter the fundamental drivers of POAF.