Calculation of biochemical analyses and 25(OH)D
TG
POLAND
Biochemical analysis of blood was carried out in an accredited analytical laboratory Diagnostics-medical laboratoriesan accredited analytical laboratory Diagnostics-medical laborator,y, Gdynia, Poland). The Sysmex XE 2100 D and XT 4000 analyzers (Sysmex Europe GmBH, Norderstedt, Germany) were used to calculate blood serum selected parameters. WBC (white blood cells), RBC (red blood cells), HGB (hemoglobin), HCT (hematocrit), and PLT (thrombocytes) were calculated by fluorescent flow cytometry technology using reagents: Cellpack, Cellsheat, Stromatolyser FB, StrolamtolyserStromatolyser-4DL, Stromatolyser-4DS, and Sulfolyser. The intra-assay CV of the method and with respect to the range were respectivelywere, respectively, as forllows: WBC 3,.0% (at least 4,.0 × 10TC (total cholesterol) was calculated by calorimetry method with cholesterol esterase reagent (Roche Cobas 6000). The intra-assay CV of the method was 1.6% with respect to the range 115.0–190.0 mg/dL. HDL-C (high-density cholesterol) was calculated by calorimetry method with HDLC4 reagent (Roche Cobas 6000). The intra-assay CV of the method was 2.2% with respect to the range ≥40 mg/dL. LDL-C (low-density cholesterol) was calculated by spectrophotometry method with LDLC3 reagent (Roche Cobas 6000). The intra-assay CV of the method was 2.5% with respect to the range 0–130 mg/dL. TG (triglycerides) was calculated by calorimetry method with Roche 11–2017 reagent (Roche Cobas 6000). The intra-assay CV of the method was 2.0% with respect to the range 0–150 mg/dL. ALT (alanine aminotransferase) was calculated by spectrophotometry method with Roche 09–-2018 reagent (Roche Cobas 6000). The intra-assay CV of the method was 3.3% with respect to the range 0–41 U/L. AST (asparagine aminotransferase) was calculated by spectrophotometry method with Roche 09–-2018 reagent (Roche Cobas 6000). The intra-assay CV of the method was 2.3% with respect to the range 0–40 U/L. CK (creatine kinase) was calculated by spectrophotometry with Roche 05–-2017 reagent (Roche Cobas 6000). The intra-assay CV of the method was 3.2% with respect to the range 39–308 U/L. The chemiluminescence (CMIA) (Liaison XL, Diasorin, Saluggia, Italy) using 25OH vitamin d total assay reagent was applied to calculate the serum concentration of 25(OH)D. The intra-assay CV of the method was 2.4–6.4%, with respect to the range; 0–20 ng/mL deficit, >20–30 ng/mL suboptimal concentration, >30–50 ng/mL optimal concentration, >50–100 ng/mL high, >100 ng/mL potentially toxic, and >200 ng/mL toxic [