3.2. Effect of Hk-BB536 on Interferon Gene Expression in PBMCs In Vitro
To further determine whether hk-BB536 affects the immune response of PBMCs, the relative gene expression levels of IFN genes, IFNα, IFNα1, IFNα2, IFNβ, and IFNγ, in PBMCs were analyzed by qRT–PCR after cocultivation with hk-BB536 or Cpg-ODN 2216 for 4 h. Cpg-ODN 2216 induced an extremely high expression of these genes compared with the control. Cocultivation with hk-BB536 significantly increased IFNγ expression (