2.3.1. Study of RPG Dietary Fiber for Bread Application
SEPARATION, BLOOD
In the study of RPG dietary fiber for bread application, there were three groups: the blank control group (white bread with no extra dietary fiber, B_C), the standard control group (3.8% pectin-added bread, B_Int0), and the RPG experimental group (3.8% RPG dietary fiber-added bread, B_RPG). Prior to conducting the main experiment, we pre-baked all samples, both with and without the addition of RPG and pure pectin, to determine whether there would be any significant alterations in the appearance and texture of white bread. And to eliminate potential bias and interference from the researchers, we ensured that the individuals responsible for producing the white bread were different from those responsible for distributing it to the study participants. This separation of roles was an essential aspect of maintaining the integrity of our research.Blood glucose level was measured using the silicon-based continuous glucose detection system (CGM) (20213070871) at 10 min before meals and 15, 30, 45, 60, 90, and 120 min after eating.Subjects were required to fill out the Appetite Assessment Questionnaires at the following time points: 5 min before the meal; every 15 min for 1 h after the meal; and every 30 min thereafter till 3.5 h after the meal. All meals were required to be eaten within 5–10 min from start to finish. For 3.5 h after the meal, no other food, water, or food-related temptations were allowed. The participants were allowed to perform simple daily activities, mainly in the sitting position.