Immunization with Ad26.Mos.HIV vaccine followed by Ad26 or MVA with gp140 induces broad viral inhibitory activity through CD8+ T cells
HIV replication
VIRUS
The capacity of vaccine-induced HIV-specific CD8+ T cells to inhibit HIV replication in autologous, infected CD4+ T cells Viral inhibition responses induced by Ad26/Ad26 + gp140 and Ad26/MVA + gp140 responses. (When considering responses to the individual isolates, the most frequently inhibited virus was the U455 clade A isolate, inhibited by 15 (94%) of the 16 vaccine participants tested, followed by CBL4 (D clade) in 13 participants (81%), IIIB (B clade) in 11 participants (69%), 247Fv2 (C clade) in 9 participants (56%), CH106 (B Clade) and ELI (AD clade) in 8 participants (50%), CH077 (B Clade) in 7 participants (44%), and ZA97012 (C clade) in 4 participants (25%) . The clade C ZA97012 isolate is matched to the viral strain of the natural Clade C gp140 protein included in the third and fourth vaccinations. The mean logWhen comparing inhibitory responses at different timepoints, logClustering of inhibitory responses revealed that HIV isolates tended to cluster based on detection of a response and response rates (In order to generate a metric for vaccine-elicited CD8+ T cells demonstrating a greater breadth of inhibition toward the panel of HIV-1 strains tested, VIA breadth scores were generated based on the number of isolates against which a positive response was elicited. For Ad26/Ad26 + gp140 and Ad26/MVA + gp140 vaccinees combined, the median number of isolates inhibited was 5 for both post 3rd and post 4th vaccination. Ad26/MVA + gp140 vaccinees inhibited more HIV-1 isolates, a median of 7 and 5 post 3rd and post 4th vaccination, respectively (Fig. S1C) than Ad26/Ad26 + gp140 vaccinees at a median 4 and 3.5 at post 3rd and post 4th vaccination, respectively. However, the differences between the 2 regimens were not significant (