2.7. Hematological–Biochemical Analysis
high-density lipoprotein, TG
BLOOD
In related to the condition of blood collection, the fasting states blood tests were assigned on the 1st day morning of the meso-cycle, which in this case were on the Mondays of the 1st & 9th week. In addition, training sessions were proceeded on Mondays through Saturdays weekly, and each Sunday was a designated resting day regardless of the weekly training load. Thus, choosing Monday as the blood-collecting day allows us to avoid possible errors due to training-related acute physiological responses. Blood samples were collected into 4.5 mL coagulation-promoting tubes. The blood samples were centrifuged at 3500 R/min for 15 min. Serum samples were analyzed using a Mindray BS420 Biochemical Analyzer (Shenzhen Mindray Scientific Co., Ltd., Shenzhen, China) to detect albumin (ALB), globulin (GLOB), total cholesterol (T-CHO), triglycerides (TG), high-density lipoprotein (HDL-C), and low-density lipoprotein (LDL-C).