Xenograft transplantation experiments
AML
AML
AML samples were obtained from patients, after informed consent in accordance with the Declaration of Helsinki. Ficoll density gradient was used to recover viable mononuclear cells. Viably frozen AML cells were thawed and CD3-depleted with biotinylated anti-human CD3 (SK7) and biotin-binder Dynabeads (Invitrogen) and subsequently injected via the lateral tail vein into 2.8 Gy irradiated (24 h before transplant) female NSGS or NRGS recipients (6–8 weeks old). For normal hematopoiesis studies, viable mononuclear cells were isolated from cord blood samples (provided by the Wesley-St Andrew’s Research Institute Tissue Bank with appropriate ethics approval) by Ficoll density gradient, CD3-depleted as above and subsequently enriched for CD34