Biomarker assays
LYSED, LYSIS, EVENT
Peripheral blood samples were collected for central flow cytometry analysis of selected B cell and T cell markers. Whole blood samples were collected in Becton Dickinson (BD) Vacutainer tubes containing sodium heparin. For cell labeling, samples were labeled with antibodies for 30 min in the dark at ambient temperature. Red blood cells were then lysed, using BioLegend RBC Lysis Buffer for 15 min at ambient temperature. After centrifugation, samples were washed with BD Stain Buffer and resuspended in 1% formalin fixative. Assay panel tubes were stored at 4 °C and acquired within 4 h of preparation.CD4 and CD8 concentrations (cells/µl) were calculated from the absolute leukocyte counts from a CBC blood sample collected at the same time using the following formula: CD4 or CD8 (cells/µl) = white blood cell concentration (cells/µl) × [(CD4 or CD8 event counts)/(white blood cell event counts)]. Extended Data Fig. Samples were acquired on BD FACSCanto II flow cytometers (BD Biosciences, designated at ILS-Dublin Canto D, s/n V33896201828) using FACSDiva software (BD Biosciences, version 6.1.3). CD19