Real-time PCR for detection of microorganism in nasopharyngeal samples
bacterial co-infection
VIRUS, SECONDARY, INFLUENZA
DNA/RNA from 200 µl nasopharyngeal specimens (repeated twice) was extracted by MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche Diagnostics, IA, US), and 100 µl of the purified DNA/RNA was aliquoted into three PCR tubes (approximately 30 µl/tube) for storage at – 80 °C.As secondary outcomes, semi-quantitative assays for measuring changes in viral load of influenza virus (types A and B) and bacterial co-infection concentrations (Detection of 