Biomarker analyses
tumor, tumors, Tumor
TUMOR, TUMORS, TUMOR
NGS of pre-treatment tumor biopsies and plasma ctDNA were performed to elucidate the landscape of genomic alterations in patient tumors. Plasma samples were collected prospectively, before treatment, and were analyzed with Guardant360 (Guardant Health). Tumor biopsies were collected after progression on last anti-cancer therapy and before treatment with the amivantamab and lazertinib regimen. Tumor biopsy NGS was performed with the Oncomine Dx Target Test (Thermo Fisher Scientific).Expression of EGFR and MET on available patient tumor samples was measured by IHC analysis of formalin-fixed, paraffin-embedded tumor tissue collected after progression on last anti-cancer therapy and before treatment with the amivantamab and lazertinib regimen. Staining for MET was performed with the anti-MET rabbit monoclonal antibody SP44; samples were run on the Dako Link 48 autostainer with FLEX detection. Staining for EGFR was performed with the anti-EGFR rabbit monoclonal antibody D38B1. Tumor cell staining was determined by the H-score method, as previously described