3′ Tag-Seq protocol
tumor
TUMOR, TAG
High-throughput 3′ Tag RNAseq was performed on frozen and formalin fixed and paraffin embedded (FFPE) tumor RNA (100 ng). We used the QuantSeq 3' mRNA-Seq Library Prep Kit (for Illumina) from Lexogen company. In contrast to traditional RNA-seq, which generates sequencing libraries for the whole transcript, 3′ Tag-Seq only generates a single initial library molecule per transcript, complementary to 3′ end sequences.