2.2. Localization of the ZED1227
ZED1227-TG2
The co-localization of TG2 and gliadin peptides raised the question of whether the ZED1227-TG2 interaction takes place already in the enterocytes. The immunofluorescence signal using monoclonal antibody A083 results from the ZED1227-TG2 conjugate because free, unbound ZED1227 is washed away from the tissue and ZED1227 does not react with other proteins. The signal was detected in villous enterocytes already at low magnification in biopsies from patients who had received the last oral dose of ZED1227 already 24 h earlier (A comparison of the integrated A083 fluorescence signal intensities for ZED1227 from biopsies belonging to the 10 mg, 50 mg, and 100 mg ZED1227 dose patient groups showed a clear dose-dependent increase in signal intensity (