2.2. Quantification of Free LA and OA in the Sesame Meal Extract
The sesame meal extract was dissolved in 50% ethanol to 10 mg/mL, clarified with 0.45 µm syringe filters, and 10 μL of the sample was injected into a Shimadzu HPLC system (Shimadzu, Kyoto, Japan) with a COSMOSIL Cholester packed column (4.6 mm I.D. × 250 mm, Nacalai, Kyoto, Japan), eluted at 1 mL/min, 40 °C. A binary gradient elution was employed with 0.1% (