Blood sampling
post-SIT, tumor necrosis
TUMOR NECROSIS
10 ml of venous blood was collected using venipuncture at different time points, including resting, immediately after completion, 45 min post-SIT (early response), and 24 and 48 h after SIT (late response). The blood sample was spun at 4ºC for 15 min at 1000 × g within 30 min of collection, and the separated plasma was stored at −80ºC and was analyzed subsequently. CK concentrations (MyBioSource, MBS269244; San Diego, California, USA), Interleukin 6 and 10 (IL-6 and IL-10), and tumor necrosis factor-alpha (TNFα) (Elabscience, E-EL-H6156, 6154, and 0109, respectively; Houston, Texas, USA) were analyzed using ELISA assays per manufacturer instructions. The coefficients for variation were <10%, and the sensitivities were 0.06 ng/mL, 0.93 pg/mL, 0.94 pg/mL, and 4.69 pg/mL, respectively.