2.4. Sample size
Sample size was calculated using PASS version 21.0.3 (NCSs, LLC. Kaysville, Utah) based on the results of our preliminary experiment, 10 participants in each group, with MABF/MMF as the primary outcome. Power analysis showed a reduction rate of 10% with α = 0.05 and a 10% dropout rate within a power value of 90%, a sample size of at least 56 per group was needed. A total of 60 samples from each group were used in this study. Figure 