Single‐cell RNA‐sequencing and data analysis
Single cell suspensions were obtained from cryopreserved sigmoid colon tissue samples through mechanical and enzymatic dissociation, and loaded onto a 10X chromium controller for cell partitioning. Single‐cell RNA‐sequencing libraries were prepared using 10X genomics NextGEM single‐cell 3′ version 3.1 reagents and sequenced on an Illumina NovaSeq 6000 sequencer. Quality control, data filtering, and data analysis was performed with the BESCA software (version 2.5.1)