2.6. Statistical Analysis
ΔCT
Comparisons between groups were made with a paired For genomic analysis, the comparative threshold (CT) cycle value for each gene was normalized according to the formula ∆CT = CT (gene)−CT (housekeeping gene). The relative gene expression levels were determined using the following formula: ΔΔCT = ΔCT sample −ΔCT calibrator. Expression fold change (FC) = 2The statistical package SPSS 22.0 software Inc. (Chicago, IL, USA) was used for the analysis and a 