Strengths and Limitations
IBD
DISEASE
This is a large dataset of phenotypically homogenous patients with IBD with established disease and provides complementary information to our previously published work in newly diagnosed patients. The combined datasets provide one of the largest series genome-wide DNA methylation data in CD to date and provides compelling replication of our previous key findings in a novel dataset of patients with established disease. The TOPPIC trial was a well-conducted randomized controlled trial performed across multiple sites across the United Kingdom with well-phenotyped data and accurate follow-up data to 3 years. Raw data were normalized together and included more than 40 technical replicate samples performed across chip positions and across separate methylation runs for each separate cohort (TOPPIC, BIOM), with appropriate clustering on MDS plots increasing the confidence of performing analyses across cohorts (