
Determination of serum indicator level

In the morning, each participant underwent a fasting period of at least a few hours before a venous blood sample of 5 ml was collected from their elbow. Immediately after the blood draw, the blood biochemistry indicators were tested. The collected blood was then centrifuged at room temperature, at a speed of 4000 rpm for 8 minutes. For further analysis of inflammatory markers, an additional 400 μl of serum was extracted and divided into two Eppendorf tubes. These tubes were stored in a refrigerator at a temperature of -70° C for later testing. The levels of various markers, including TNF- α, IL-1β, IL-6 and IL-15 were quantified using competitive inhibition enzyme-linked immunosorbent assay (ELISA) kits (Thermo Fisher Scientific, Invitrogen, Waltham, MA, USA) [