Statistical analysis
All data were presented as mean ± SD. The statistical analyses were accomplished with Statistical Package for the Social Sciences (SPSS) (version 25.0 software; SPSS, Chicago, IL); and graphs were drawn with GraphPad Prism version 9 software. To confirm the normal distribution of data, the Shapiro–Wilk test was accomplished (p-value > 0.05 was considered as the normal distribution). The one-way repeated measures analysis of variance (ANOVA) was utilized to examine the trend of variables over time between the two groups. Then to compare the two test and control groups at separate times, the independent t-test and Mann–Whitney test were used if the variable was normal and non-normal, respectively. If variables were normal, comparing pre- and post-measurements was done by the dependent t-test, and the Wilcoxon test was used, otherwise. Also, a p-value < 0.05 was considered statistically significant