Cytokine response
We measured the levels of IL-12 in patient’s blood after Ad5-IL-12 adenoviral injection. Since IL-12 secretion causes IFN-γ production by NK, NKT, activated CD4+ Th and CD8+ Tc cells and subsequently CXCL10 by monocytes, fibroblasts, and endothelial cells, the expression levels of IFNγ and CXCL10 were also measured. The blood was drawn before viral injection, on the day of the adenoviral injection and on prescribed days up to 42 days and cytokines were quantitated by serum ELISA. Similar to our earlier observations [