Biochemical tests
All biochemical parameters will be tested out at an accredited laboratory (Nawaloka Metropolis laboratory, Nawaloka Hospital PLC) following the standard procedures.
Full blood count: This will be analyzed using SYSMEX XE-2100 Haematology Automated Analyser.HS-CRP: The Roche Cobas c501, which is an in vitro diagnostic test system, will be used to quantitatively determine the C reactive protein (CRP) in human capillary whole blood and serum, EDTA K2/K3 and lithium heparin anticoagulated whole blood and plasma by photometric measurement.Serum vitamin D3: The serum will be separated from the 5.0-mL venous blood sample collected and tested within 3 h using MAGMLUMI 2000 analyzer by Competitive Chemiluminescence Immunoassay (CLIA). This test quantitatively measures the sum of both 25-(OH) vitamin D3 (cholecalciferol) and 25-(OH) vitamin D2 (Ergocalciferol) in the specimen.