Blood sampling
bleeding, acne, hirsutism
BLEEDING, ACNE, HIRSUTISM
At baseline on day 3 of a spontaneous menstrual period or a withdrawal, bleeding was induced by medroxyprogesterone acetate in patients with irregular cycles without recent menses, and upon pregnancy, it was monitored weekly by urinary human chorionic gonadotropin test, or at the end of the 16 weeks of treatment, fasting blood samples were collected for measurement of metabolic parameters, lipid profile, and serum ions in those patients who are not pregnant at that time.The anthropometric, degree of hirsutism and acne were collected and body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared. All biochemical assays were carried out in a core laboratory after an overnight fast. All fasting blood collected at baseline and after intervention was used for metabolic parameters and serum ion assays. These were measured in samples stored at −80°C using an ELISA assay with no pre-dilution.