HLA antibody testing and HLA typing
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HLA antibody testing and HLA typing were performed before transplantation. Specificities of antibodies were reported by LABScreen® Single Antigen Class I and Class II assay (One Lambda Inc., Canoga Park, CA). Single antigen beads were used to test for antibodies against HLA-A, -B, -C, -DRB1, -DRB3, -4 and -5, and -DQB1. Using BioSewoom™, typing of low-to-medium resolution HLA-A, -B, -C and DR was done. PCR/SSP kit (BioSewoom Inc., Seoul, Korea) and high resolution HLA-DQB1 typing was performed by AVITA™ plus HLA-DQB1 SBT kits (BioWithus Inc., Seoul, Korea).After transplantation, LABScreen® Single Antigen Class I and Class II assay was performed every one or two months and when there was an acute deterioration of renal function.