Introduction
T2D, CVD, MetS, metabolic syndrome, type 2 diabetes
CVD, METABOLIC SYNDROME, TYPE 2 DIABETES, CARDIOVASCULAR DISEASE
The metabolic syndrome (MetS) is a cluster of conditions highly predictive for the subsequent development of type 2 diabetes (T2D) and cardiovascular disease (CVD) [Analysis of the metabolomic complexity of MetS is most frequently conducted on the fasting state, although it is apparent that disordered metabolic flux is evident following nutrient ingestion [Therefore, the aim of this study was to examine the complex metabolomic responses to mixed test meals, containing either high glycaemic index (HGI) or low GI (LGI) carbohydrates in a cross-over study. For this, a cohort of female participants selected on the basis of the presence or absence of MetS were recruited. Analysis was undertaken of metabolome profiling using hydrophilic interaction chromatography (HILIC) coupled with high-resolution mass spectrometry (HRMS). Based on the available literature, it was hypothesised that using an exploratory metabolomics approach, dynamic alterations in amino acids and related metabolites would be identified and these metabolites would be a key discretionary feature of the altered circulating metabolomic responses to carbohydrate-rich meals [