TCR sequencing of DSG3-specific T cells.
pemphigus
PEMPHIGUS
Cryopreserved PBMCs from a patient with pemphigus were thawed and rested for 4 hours at 37°C. Cells were stimulated with 5 μg/mL recombinant human DSG3 protein (Cusabio) for 20 hours at 37°C in a 5% CO