SARS-CoV-2 spike-pseudotyped virus neutralization assay
subvariants BA.4
VIRUS
SARS-CoV-2 nAb in samples were assessed using a validated SARS-CoV-2 spike (S)-pseudotyped virus neutralization assay (PsVNA) in 293/ACE2 cellsThe spike-pseudotyped viruses were derived from ancestral Wuhan-Hu-1 with the following amino acid substitutions: (prototype (D614G); Omicron subvariants BA.4 and BA.5 (designated BA.4/BA.5 for identical spike sequences between BA.4 and BA.5 (T191, L24S, ΔP25, ΔP26, ΔA27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681G, N764K, D796Y, Q954H, N969K])); Omicron subvariant BQ.1.1 (T19I, L24S, ΔP25, ΔP26, ΔA27, H69-, V70-, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K); Omicron subvariant XBB.1 (T19I, L24S, ΔP25, ΔP26, ΔA27, V83A, G142D, Y145Q, ΔH146, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K)).