2.6. OVA-Induced AHR Mouse Model
Allergic airway inflammation
Seven-week-old female BALB/c mice were purchased from The Experimental Animal Facility of the College of Medicine, National Taiwan University (Taipei, Taiwan). Allergic airway inflammation was induced with an intraperitoneal injection of 50 µg of OVA (purchased from Sigma-Aldrich) mixed with the Th2-adjuvant aluminum hydroxide on day 0 and was followed by the administration of 25 µg of OVA on days 14, 28, 42, and 56 to sensitize the mice and then by intranasal challenge with OVA (100 µg) on days 67 and 68. Sera were collected from the retro-orbital sinus on the day before the first OVA sensitization; on post sensitization days 35, 49, and 63; and on the day of sacrifice. 