Monitoring of CAR-T engraftment by PCR
Woodchuck Hepatitis
CAR-T cell engraftment was assessed in peripheral blood by a duplex qPCR amplification assay targeting the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE), a sequence part of the vector encoding the CLDN6-CAR and an intronic sequence from FOXP2, which serves as a reference for normalization of cell numbers. Genomic DNA (gDNA) was isolated from cell pellets, and DNA concentration was measured and corrected to a target concentration of isolated gDNA of 125 ng µl