Study procedures: PBMCs isolation and mitochondrial respiration
OXIDATIVE STRESS
For mitochondrial function, twenty mL of blood was collected from all participants. In order to isolate PBMCs, a Ficoll density gradient centrifugation was used. Measurements of cellular oxidative stress, mitochondrial oxidative stress, and mitochondrial mass were performed using PBMCs. In a brief, after the initial centrifugation (1000