Results
tumor, corneal ulcer, diabetes mellitus, dysplasia, tumors, allergy, CIN
TUMOR, INTRAEPITHELIAL NEOPLASMS, CORNEAL ULCER, DYSPLASIA, DIABETES MELLITUS, TUMORS, OPACITY, CIN, PUNCTAL STENOSIS, ALLERGY, FOLLICULAR CONJUNCTIVITIS, HUMAN IMMUNODEFICIENCY VIRUS, CONJUNCTIVAL INTRAEPITHELIAL NEOPLASIA, CARCINOMA IN SITU, CONJUNCTIVA, ARTERIAL HYPERTENSION
Assuming a dropout of 20%, 9 subjects per group were included, enrolling a total of 18 patients with clinical diagnosis of CIN. Nonetheless, one subject from the IFNα2b group was excluded from the analyses as they were later histopathologically diagnosed with invasive SCC. From the remaining subjects, 7 were male, and 10 were female, with a range of age of 50–90 years, and an average age of 70 years ± 11.91. MMC was administered to 9 patients, and IFNα2b to 8 patients. Basal impression cytology was positive in 15 patients (83.3%). Regarding the degree of dysplasia, 7 were reported as mild (16.7%), 7 as moderate dysplasia (38.9%) and 1 as severe (5.6%). All patients were staged as with carcinoma in situ (Tis) using the AJCC classification system. Demographic and clinical characteristics of the included patients in MMC and IFNα2b groups are presented in Table 
Summary of demographic and clinic characteristics from the included patients with intraepithelial neoplasms of the conjunctiva and corneaHIV, Human Immunodeficiency Virus; IFNα2B, Interferon alpha 2B; MMC, mitomycin C; †, including diabetes mellitus and arterial hypertension; *, Student´s t; **, Fisher exact test.All 8 patients of the IFNα2b group presented complete resolution of the tumor and no relapses were recorded during the follow-up. Similarly, the subjects receiving MMC also showed complete response to topical therapy; however, one patient presented a relapse 8 months after start of treatment and went through surgical excision. A consistent reduction in the size of tumor was observed in the MMC group throughout time (p < 0.0001). The first significant differences in tumor sizes occurred between week 0 (33.33 ± 24.29 mm
Effects of topical chemotherapy in the size of conjunctival intraepithelial neoplasia. Mean tumor size and percentage of tumor reduction are presented. A consistent reduction in the size of tumor was observed in the MMC group throughout time (The longest time of resolution for IFNα2b was 34 weeks, whereas for MMC was 14 weeks. The mean time for total resolution in the MMC group was 59.11 ± 24.02 days, while for IFNα2b it was 143.50 ± 47.181 (
Results of topical chemotherapy for conjunctival intraepithelial neoplasia. Representative cases of topical chemotherapy with MMC (On the other hand, survival analysis of CIN treated with topical chemotherapies revealed that the median survival was 10 weeks for MMC and 20 weeks for IFNα2b group. Survival curves were statistically different (P < 0.0001). The Hazard Ratio (log-rank) was 4.182 (IC 95% 1.307 to 13.38; p < 0.0001) and showed that the tumors treated with MMC have a higher chance to resolve earlier than the treated with IFNα2B. Kaplan-Meier survival plot is presented in Fig. 
Survival analysis of conjunctival intraepithelial neoplasia treated with topical chemotherapy. The median survival was 10 weeks for MMC and 20 weeks for IFNα2b groups. Survival curves are statistically different (P < 0.0001). MMC; mitomycin C, IFNα2b; interferon alpha-2bLastly, the pharmacological safety of both drugs was similar. Severe side effects, such as corneal ulcer or corneal opacity, were not recorded in any patient; however, the number of reported side effects was greater in the MMC group than in the IFNα2b (16 vs. 5). There were 4 major side effects in IFNα2b and 3 major side effects in MMC. Punctal stenosis and allergy were reported only in the MMC group, whereas follicular conjunctivitis and corneal epithelial microcysts were only observed with IFNα2b topical therapy. Nonetheless, the number of side effects per patient was non-statistically different between groups (
Reported side effectsIFNα2B, Interferon alpha 2B; MMC, mitomycin C; NA, Not Applicable