Fecal microbiota transplantation in pseudo-sterile mice
STERILE
The animal experimental protocol was approved by the animal ethics committee of Jinan University (20190821-02). A total 50 of C57BL/6J mice (weight, 25.00 ± 2g; age, 10 weeks) were purchased from Biotechnology Company Limited (Beijing, China). The mice were raised in the specific pathogen-free animal experiment center at Jinan University, with constant temperature (24 ± 2°C), constant humidity of 55 ± 10% and a 12-h light/dark cycle. All mice received adaptive feeding for 7 days, and then were randomly divided into a control group (NC, n=10) and an antibiotic treatment group (AT, n=40). Within the next 4 weeks, the mice in AT group were fed with antibiotic-containing sterile water and then divided into 4 groups. More detailed information was shown in 