4.3. Limitations
Additional limitations to those noted above include the lack of direct knowledge of total body water or any of its component volumes other than plasma volume. These would have required tracer dilution techniques and time, while the volume of glycogen-bound water released during glycogenolysis is more problematic to estimate. Similarly, no hormones were measured to provide more insight into fluid regulatory mechanisms across the four trials. These limitations reflect mostly a limited research budget and demanded priorities placed on participants, along with the need to limit confounding effects between different measures.