4.13. VEGF Polymorphism
DNA was extracted from the buffy coat of 47 patients, and 5 ng of genomic DNA was used to measure single nucleotide polymorphisms at the following sites: rs833061, rs699947, rs25648, rs4604006, rs2071559, rs2305948, rs2010963, and rs664393 using TaqMan SNP Genotyping Assays. Briefly, 5 ng of genomic DNA was used for each qPCR reaction in a final volume of 10 µL on the ABI 7500 fast machine. Genotype assignment was performed using the ABI 7500 software (Version 2.0.4).