Analysis of blood
STERILE, CLOT
During the initial visits (at baseline assessment) and experimental visits (min 0, 30, 60, and 90), venous blood was collected from the antecubital forearm vein by trained phlebotomists under sterile conditions. Per time point, blood was drawn into vacutainer tubes coated in KWhole blood drawn into the serum vacutainer was immediately separated into micro‐hematocrit capillary tubes (Thomas Scientific) and analyzed for hemoglobin concentration (OSM‐3, Radiometer) and, after centrifugation, hematocrit percentage. The remaining blood from the serum vacutainer tube was allowed to clot at room temperature for 30 min, then centrifuged for 15 min at 3500 rpm at 4°C. Serum was then separated into aliquots and stored at −80°C for later batch analysis.Change in plasma volume was calculated using the Dill and Costill method (Dill & Costill, 