PRP preparation [
STERILE

26 ml of blood was obtained from each patient (6ml each in 4 CPDA (citrate phosphate dextrose adenine) and 2ml in EDTA (ethylene diamine tetra acetic acid tube) for CBC).First-spin of 900 g for 5 min (Kokusan, H-19alpha, 25 °C).Three layers formed: transfer top layer (platelet poor plasma) and middle layer (platelets and WBC) to another sterile tube (about 3.3 ml), discard bottom layer (RBC).Second-spin of 1000 g for 10 min.The upper one third was discarded (platelet poor plasma) by pipet, the remaining was mixed by turning 10 times.Final product was 4 ml of PRP (3ml for procedure, 1ml for cell differentiation and culture).
Four tubes of CPDA were collected with one EDTA tube. First-spin product. Machine used for PRP preparation. Second-spin product. 3 ml of PRP for transforaminal epidural injection