2.8. Biomarkers and biochemical analysis
STERILE
Fasting blood samples (approx.12.0 ml) were collected in collection tubes, serum was separated and stored at −70°C until use. Random urine samples (approx. 10 ml) were collected in sterile urine sample containers and analyzed within 24 hours. The participants were asked not to drink or eat anything for 60 minutes before saliva collection. Salivary samples were collected at 8.00 Nitric oxide, Glutathione, and Malondialdehyde in serum were assessed by colorimetric assay, salivary cortisol (8 