2.2.3. Quantitative evaluation of wrinkles.
The same images obtained for expert evaluation will be analyzed by the Image J software to quantify wrinkles. They will be quantified by the variation of pixels in the image of frontal wrinkles, periorbital, glabella, and nasolabial folds before and after 1 month of treatment. The ImageJ software analyzes the image by the intensity or gray level of the pixels. The calculation of the areas is done by counting the pixels of the selected regions.