Pairwise analysis
Pairwise analysis was performed to assess the dynamic range of informative elements across the entire set of TME cellular and molecular features in each SNF patient group. We compared baseline and on-treatment data from the three assays described above (13 patients). In the CyTOF data layer, patients in cluster 1 exhibited relatively increased expression of NKG2D in naïve, memory CD4 T cells and Tregs, which normally express very low levels of NKG2D. It is possible that their low frequency overstates the high-fold change in the pairwise analysis.A relative over-representation of T-cell activation markers such as HLADR, T-bet and PD-1, and CD103+ dendritic cells (specialized in cross-priming) was observed in cluster 1 vs the other two clusters, suggesting an activated adaptive immune signature that may be associated with response (Pairwise longitudinal analysis of 