Participants
acute myocardial infarction, pneumonia, cancer, diabetes mellitus, allergy, asthma
GENERALIZED PERIODONTITIS, ACUTE MYOCARDIAL INFARCTION, PULMONARY EMBOLISM, PNEUMONIA, CANCER, RENAL FAILURE, DIABETES MELLITUS, ALLERGY, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, CONGESTIVE HEART FAILURE, ASTHMA, BLOOD
All individuals attending the Unit of Periodontology at the University São Leopoldo—MANDIC (Brazil) were screened for eligibility. Subjects were eligible to participate if they met the following inclusion criteria: (i) age 18 years and above; (ii) Fit and well, without current or previous diagnosis of diabetes mellitus (Glycosylated haemoglobin > 6.5%), confirmed by blood Basal insulin and Fasting glucose levels; (iii) Patients requiring periodontal treatment diagnosed to stage 3 or 4 generalized periodontitis, grade B or C; (iv) Body Mass Index (BMI) within range of normal to overweight (BMI ≥ 18.5 ≤ 29.9 kg/m2). All participants were provided with the subject information sheet and gave written informed consent, which was followed by collection of medical and dental histories, Blood, and Gingival Crevicular Fluid sample collection and comprehensive oral examination.We excluded participants undergoing cancer treatment; taking immunosuppression medication; alcoholics; smokers; with renal failure; liver changes; congestive heart failure; history of acute myocardial infarction less than 6 months ago; respiratory changes (pneumonia, pulmonary embolism, asthma, chronic obstructive pulmonary disease); allergy to any component of the medication formula; when using any medication that may interfere with the protocol of treatment, for example anti-inflammatories or antibiotics; and individuals who do not agree to sign the informed consent.