Microbial compositional alterations of the gut microbiota
TYPE 2 DIABETES
The raw data obtained from the Illumina MiSeq platform were quality-filtered and demultiplexed to remove invalid and low-quality sequences. A total of 2170797 quality-filtered and chimera-checked sequences were obtained from the 34 samples. The rarefaction curves demonstrated that the sequencing depth was enough for the microbial diversity analysis in the current study (The diversities of gut microbiota before and after the high-fiber diet consumption were analyzed, and the CS and CE groups revealed the start and end phases in the control group, while the TS and TE groups revealed the start and end phases in the treatment group. The rarefaction curves To assign taxonomic compositions of gut microbiota, the RDP classifier was used to compare the bacterial community structure shifts at the phylum level and genus level, respectively. Taxonomic analysis revealed that a total of 15 phyla, 25 classes, 63 orders, 119 families, 307 genera, and 603 species were identified. At the phylum level, Firmicutes and Bacteroidota account for 79.96% percentages of the total bacterial communities (The bacterial communities at the phylum levels Heatmap of hierarchy cluster results for the abundance of genus in control group and treatment group. The color of the spots corresponded to the normalized and log-transformed relative abundance of the OTUs. The genus names of the OTUs are shown on the right.PICRUSt functional prediction was performed using EggNOG database, pathways related to type 2 diabetes were identified and compared.