3.2. Urinary Excretion of HT Metabolites
HT
The unmetabolized form of Ole was almost undetectable in the urine before and after ingestion of the respective IPs. Free (unchanged) HT was excreted within an hour and in small amounts after the ingestion of IP-1 and IP-2 (0.0004 µmole ± 0.0010 and 0.0024 µmole ± 0.0009, respectively; no significant difference between groups), but not after EVOO intake (A dose-dependent concentration of all the HT metabolites analyzed was recorded following the IP administration (The HT-4-S and the glucuronide conjugates were measured after intake in all the interventional groups, but in smaller concentrations than HVA, DOPAC, and HT-3-S. Unlike IP-1, the EVOO and IP-2 groups, containing the lowest and highest dose of HT, respectively, showed a predominant detection of HT-glucuronides over HT-4-S (not significant for EVOO, and The mean HT excretion calculated from the accumulated amounts was estimated at 59.6% and 35.8% of the total intake for IP-1 and IP-2, respectively, and 27.6% when administered with HT-enriched EVOO.