Immunophenotyping and cytotoxicity
MP, Tumor
CYTOTOXICITY, TUMOR
Immunophenotypic profiling of MP and PBMC samples was performed by flow cytometry, using multicolor staining panels (CD3 (SK7), CD4 (SK3) and CD8 (SK1), BD Biosciences; CD45RA (HI100) and CCR7 (G043H7), BioLegend; and Live/Dead Fix Aqua, Thermo Fisher Scientific). For all samples, in the CD3Functionality of T cells in samples of MP administered were profiled in vitro using an exploratory cellular cytotoxicity assay. Tumor cell growth (A375nucGFP