Stimulated whole human saliva collection
Healthy adults, who agreed to donate saliva, chewed on a paraffin wax for 10 min and all the stimulated saliva was collected in chilled vials. The saliva collected from 15 donors was immediately pooled. Then, the saliva pool was centrifuged (20 min; 4 °C; 4,000g) and the supernatant was distributed in small aliquots, which were stored at − 80 °C until use. This procedure was performed in the morning and the volunteers were asked not to eat or drink anything, except for water, for 2 h before the saliva collection