Exploratory peripheral analyses
toxicity, MP
Afami-cel MP showed in vitro cytotoxic activity in all batches before dosing. In vitro killing of MAGE-A4Persistence of afami-cel was detected in all post-infusion blood samples up to 18 months after treatment. Peak afami-cel persistence was reached in most patients within the first 7 days after infusion. Integration site analysis evaluated clonality status. In five patients with persistence >1% of peripheral blood mononuclear cells (PBMCs) 1 year after infusion, afami-cel showed a high level of polyclonality and absence of clonal dominance (Supplementary Table To assess whether long-term persisting afami-cel retains its functional cytolytic capacity, a sample was taken ~9 months after afami-cel infusion from a patient with SS with durable PR for 28 weeks who eventually progressed. CD8Longitudinal changes in memory subset populations in the transduced CD4A panel of 22 serum cytokines was simultaneously assessed in mITT patient samples collected before infusion and after infusion to understand potential mechanisms of toxicity and efficacy. A transient post-infusion increase was evident for most serum cytokines across all patients, with the largest increases in interferon γ (IFNγ) levels. Serum IFNγ (Fig. 