Cytokine analysis
TNF-α
PANCREATIC CANCER
To evaluate modulation of cytokines by NEO-201, serum samples were collected at timepoints before and after treatment and analyzed by ELISA-based cytokine array. At 24 h from infusion, serum IL-10 and TNF-α levels were increased in all patients at all dose levels. IL-10 increased to median fourfold higher in all patients compared to pre-infusion, and TNF-α to median 4- to sixfold higher in all patients (Fig. Serum cytokines modulation after NEO-201 infusion. Serum cytokines were evaluated using the V-PLEX Proinflammatory Panel 1 Human Kit. The figure depicts modulation of serum levels of cytokines statistically significant after NEO-201 treatments at different time points compared to baseline levels before treatment (C1PRE). No change in IL-12p70, IL-13, IL-1β, IL-2, IL-4 levels were observed in these patients after NEO-201infusion at all time points in all cohorts (data not shown). Effects on IL-6 were variable, with elevated median serum IL-6 levels detected in 3 patients (2 from 1.0 mg/kg, 1 from 2.0 mg/kg) at 24 h post-infusion. IL-6 levels returned to baseline levels at 72 h post-infusion, except for in one patient with pancreatic cancer in the 2.0 mg/kg cohort in which IL-6 levels remained elevated until C1D15 (Fig. 