Vaginal Epithelium Thickness
INFILTRATION
To assesses vaginal epithelium thickness (VET), 2 full-thickness vaginal mucosal samples taken from the right vaginal wall 2 to 3 cm above the introitus were obtained using Tischler biopsy forceps after local lidocaine infiltration. One of the specimens was fixed in formalin and routinely embedded in paraffin for histological evaluation, and 4-μm sections were stained with hematoxylin and eosin and digitized using a IntelliSite Ultra-Fast Scanner (Philips). The slides were evaluated and measured by a gynecologic pathologist. VET was microscopically evaluated by calculating the mean of the 3 areas showing the maximum VET and the 3 areas demonstrating the minimum VET in hematoxylin and eosin–stained tissue samples.