Sample size and statistical analysis
β=0.10
After obtaining ethical approval, we conducted a preliminary experiment to verify the feasibility of the experimental design and obtained IONM data to further calculate the sample size. It was anticipated that the mean values of IONM amplitude in the three groups would be 1283, 1203, and 1028, respectively. The corresponding standard deviations for each group were 295, 267, and 283. One-Way Analysis of Variance F-Tests were performed with α=0.05 and β=0.10. Using PASS 15 software, a sample size of N=102 was calculated, considering a potential loss-to-follow-up and refusal rate of 20%. Thus, a minimum of 129 samples (43 individuals per group) were deemed necessary for the final analysis.SPSS 26.0 statistical software (IBM Corporation, USA) was used for analysis. For normally distributed measurement data, results were expressed as 