3.2. Anthropometric and Biochemical Values after the Dietary Intervention and BMI Loss Prediction Model for the MHP and LF Diets Based on DNA Methylation Data
Of the 306 participants, 73 subjects did not complete the dietary intervention, and 32 participants had low adherence to the diets, obtaining post-intervention data from 201 participants: 93 on the MHP diet and 108 on the LF diet (The statistical analyses of the changes in the anthropometric and biochemical variables in response to dietary treatment after 4 months of intervention are shown in The analysis of the changes after the dietary intervention showed that the HDL-cholesterol in the participants with the LF diet presented a significantly greater increase than in the participants with the MHP diet (A prediction model based on basal DNA methylation data was designed to determine the percentage of BMI loss for each individual. The two types of diets and the CpG sites with methylation levels at the baseline best associated with BMI reduction were used as predictors.A selection of methylation sites was made from the 201 participants who completed the dietary intervention and had good adherence to the diets (Spearman’s correlation was performed between methylation of the selected 1233 CpG sites and the percentage of BMI loss for each of the diets after 4 months of intervention, selecting those CpG sites that presented a significant correlation with To better predict the percentage of BMI loss with each of the diets, the “furnival-Wilson leaps and bounds” algorithm (“vselect” in Stata) [With these requirements, 15 CpGs for the MHP diet and 11 CpGs for the LF diet were identified. 