Hyaluronic acid gel
The gel was prepared at the faculty of Pharmacy, Alexandria University, Egypt. To prepare 10 g of HA gel (2%), 0.2 g of high molecular weight (HMW) HA (Sigma, 99% purity) was dispersed in 10 mL deionized water at low pH (3.5) while stirring with a magnetic stirrer (IKA, UK) at 600 rpm for 25Gel characterization was done as following: Viscosity measurement, Spreadability test and mucoadhesiveness, Invitro release (Using dialysis diffusion method) and stability testing [The concentration of HA gel used in the current clinical trial was 2% based on the final report of the safety assessment of hyaluronic acid, potassium hyaluronate, and sodium hyaluronate published in the International Journal of Toxicology [