External validation, RT-qPCR and immunohistochemical analysis
To further validate the role of five key genes (UCA1, FNDC3B, MYBL2, NHS, CCDC15) in the development of PCa in the risk model, the GSE57495 dataset was used as external validation. The Kaplan–Meier analysis showed that, as expected, patients in the HDR subgroup had significantly shorter OS than those in the LDR subgroup (The results of external validation and RT-qPCR (