2.8. Physical Activity and Sleep
ActiGraph GT3X+ (Actigraph Inc., Pensacola, FL, USA), a triaxial accelerometer, was used to collect movement behavior data. The participants were required to wear the accelerometers for 8–9 days on their non-dominant wrist before the first (week 0) and last (week 8) visits, allowing for 7 complete days of continuous, 24 h data capture. Participants were included if they recorded minimum of 16 h of valid wear time per day for at least two valid weekdays and one weekend accelerometer data. Data were converted using the ActiLife software (version 6.13) and processed using the GGIR package (version 2.0). Night sleep duration was calculated using GGIR default algorithm and non-sleep time was classified into inactivity and light-intensity, moderate-intensity, and vigorous-intensity physical activity (<25, 25 to <100, 100 to 430 and ≥430 milligravity (mg), respectively, 1 mg = 0.00981 m.s