2.4. Intervention
The test soft capsules included 6 mg astaxanthin, 10 mg lutein, and 2 mg zeaxanthin (66 mg/capsule: Hematococcus-pluvialis-derived pigment [BGG Japan Co., Ltd., Tokyo, Japan) and 55 mg/capsule: marigold [BGG Japan Co., Ltd.]). The placebo was a soft capsule containing rice oil instead of these ingredients. The subjects took either one test soft capsule (active group) or one placebo capsule (placebo group) daily for eight weeks. According to previous clinical studies, 6 mg/day astaxanthin consumption restores the accommodative function and improves ocular symptoms in VDT operators [