2.1.3. Enzyme-Linked Immunosorbent Assay (ELISA)
PBMCs seeded in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin were treated with 10 μg/mL LG2055 and 0.1 μM ssRNA (ORN R-0006; Miltenyi Biotech, Bergisch Gladbach, Germany)/DOTAP for 24 h at 37 °C and 5% COThe concentration of IFN-α in the supernatant was measured using an ELISA Flex: Human IFN-α (HRP) kit (MABTECH, Nacka Strand, Sweden) according to the manufacturer’s instructions. Each experiment was performed in triplicate.