Cytokine assays
Mouse serum was obtained by centrifugation at 1000x g for 20 min at 4 ° C, after which serum cytokine concentrations were measured using ELISA kits for IL-1β(Cat#SEA563Mu; mouse; Cloud-Clone Crop; sensitivity range: 15.6–1,000 pg/mL; concentration range used for generating calibration curves: 1,000, 500, 250, 125, 62.5, 31.2, 15.6 and 0 pg/mL) and TNF-a (Cat# SEA133Mu; mouse; Cloud-Clone Crop; sensitivity range: 15.6–1,000 pg/mL; concentrations used for generating calibration curves: 1,000, 500, 250, 125, 62.5, 31.2, 15.6 and 0 pg/mL), according to the manufacturer’s instructions.