2.5. Statistical Analysis
INTERACTION
All experimental data are shown as mean and standard deviation (±SD). Time-course changes in blood variables were evaluated using two-way analysis of variance (ANOVA) with repeated measures. Interaction effect (trial × time) and main effects were analyzed. When a significant interaction or main effect was detected, a post hoc Tukey–Kramer test was performed to determine where specific trial differences existed. Statistical significance was accepted when 