Flow cytometry
For ADCC assays, after NK cells were co-cultured with Ba/F3 cells for 4 hours, all cells were collected and rinsed by PBS. Cells were incubated by Propidium Iodide Staining Solution (BD,# 556463) for 15 minutes at room temperature. For EGFR expression on the plasma membrane, Ba/F3 cells after 6 h or 24 h treatment (10 ug/mL JMT101, 100 nmol/L EGFR TKIs or 100 nmol/L EGFR TKIs plus 10 ug/mL JMT101) were collected and rinsed by PBS. Cells resuspended by PBS were incubated by Alexa Fluor 488 anti-human EGFR (5ul/test; BioLegend, #352908, lot: B332782) for 15 minutes at room temperature. Alexa Fluor 488 Mouse IgG1, κ Isotype Ctrl (FC) Antibody (5ul/test; BioLegend, #400129, lot: B354284) were used as isotype control. Data were processed using FlowJo v10, BD FACSDiva Software v8.0.1 and CytExpert v2.4.