Limitations
REGRESSION
The small sample size of the control group limits the power of detecting treatment-by-subgroup interaction effects. Thus, more subtle differences of psilocybin-induced effects between ADE and control animals might potentially be undetected. Taking into account the broad distribution of the ADE phenotype and its clear association to the neuronal effects of psilocybin demonstrated in our study, future studies are needed to delineate how the intensity of the ADE phenotype exactly blunts the effects of psilocybin. Importantly, the unequal sample sizes did not affect our statistical analyses. To control basic assumptions of the repeated measures ANOVA, we integrated Shapiro–Wilk [To rule out possible confounders of the study, such as alcohol-induced changes in tolerance regarding anesthesia or alterations of 5-HT receptor expression in ADE rats, several control analyses and experiments were performed. To exclude cross-tolerance effects between ADE rats and anesthesia during scanning, we conducted a baseline comparison between ADE and control rats at the first time point of measurement (Supplementary Fig. We did not apply global signal regression (GSR) in our rsfMRI analysis due to several inherent caveats of this technique: GSR is a method originally developed to account for potential sources of physiological noise [