Phagocytosis
EVENTS
Phagocytosing ability was measured in 1 × 10Samples were then put on ice and ice-cold PBS was added to stop reactions. Tubes were then centrifuged at 3,200 rpm and resuspended in PBS containing 2% FBS. 3µL DRAQ7™ dye (Abcam, Cambridge, UK) was added as a viability marker and dead cells were quantified and excluded through DRAQ7™ inclusion. The samples were analysed using the BD Accuri flow cytometer (BD Biosciences, Stockholm, Sweden), with pHrodo™ and DRAQ7™ excited with the 488 nm laser and 640 nm lasers then detected in the FL-2 and FL-4 channels, respectively. 20,000 gated events were analysed.