HIV-1 infection of PHA-blasts and monocyte-derived macrophages
Isolated PBMCs from HIV-1 uninfected donors were stimulated with 5 μg/ml Phytohaemagglutinin (PHA, Sigma-Aldrich) and 10 U/ml IL-2 (Roche). After 3 days, PHA-blasts were infected with the HIV