Cell-mediated immune response by intracellular cytokine staining
PBMC were thawed and suspended in RPMI 1640 medium supplemented with Minimum Essential Medium non-essential amino acids: L-glutamine, penicillin/streptomycin, sodium pyruvate, 2-mercapto-ethanol (all from Invitrogen), and fetal bovine serum (Seradigm). Then the PBMCs were incubated The norovirus-specific CD4