NKT cell response
To evaluate the cellular response to α-GalCer, NKT cell counts were measured in PBMCs by flow cytometry using fluorescent α-GalCer-loaded human CD1d tetramers. The gating strategy is provided in Supplementary Fig. 3. Data are expressed as number of NKT cells per 100 CD3Analysis of NKT cell responses at Stage I. Comparison of cytokine levels for DCV + 