2.8. Statistical Analyses
EVENTS, SIDE EFFECT
Primary outcome measures included cognitive performance based on the Serial Sevens test (number of errors made, number attempted), N-Back test (score, number of correct responses, number of attempted responses, accuracy (%), and average response time per attempt), and the Go/No-go Test (percent correct on Go, percent correct on No-go, reaction time on Go, reaction time on No-go). Secondary outcome measures included subjective changes in mental clarity, mood, focus, concentration, productivity, ability to tolerate stress, and subjective changes in happiness. Tertiary outcome measures included vital signs (blood pressure and heart rate), body weight, and side effect profile/adverse events monitoring. An a priori power analysis was conducted for the changes in VAS between treatments using G*Power. For a mixed factorial ANOVA with repeated measures, with three groups and three time points, within-between interaction, and a small effect of 0.25, a sample size of 36 was needed to achieve 80% power. All variables were tested for normality using results from a Shapiro–Wilk test. Data are presented as means ± standard deviation, and the primary statistical approach employed was mixed factorial ANOVA with repeated measures on time to assess group (AMT vs. LM vs. PL), time (baseline vs. 1 h vs. 2 h), and group x time interaction effects for all for cognitive performance testing, subjective changes in VAS and SHS indices, and vitals. A one-way ANOVA with repeated measures was completed to assess differences between conditions/groups for BW. When sphericity was violated, the Greenhouse–Geisser correction was used to adjust the p-values for main effects/interactions. Tukey post hoc procedures were used to assess individual comparisons between time points and/or groups. Main effects and interactions that indicated a significant difference (