Cytotoxicity assay
CYTOTOXICITY
Before the clinical application of the Carie-Care™ gel, its cytotoxicity was assessed in human gingival fibroblasts isolated from patients who underwent clinical crown lengthening, and signed informed consent was obtained from all participants. At a cell density of 7 × 103/well, gingival fibroblasts from passages to 4–6 were seeded onto a 96-well plate and incubated for 24 h in 5% CO