Participants
All male members of the Koohpaye Box club were invited to participate in this study. The Koohpaye Box club is one of the top CrossFit gyms in Tehran City, Iran. This study was conducted in one CrossFit club to eliminate the potential effects of variations in training programs between different clubs. CrossFit participants were included in this study if they had at least 2 years of experience in CrossFit, did not consume any supplements 3 months before and during the study, and were not heavy CAF users (CAF ≤125 mg/d). A total of 20 male CrossFit participants (age: 22.30 ± 2.88 years; height: 1.78 ± 6.84 m; body mass: 80.6 ± 12.05 kg) completed the study. To determine whether the number of participants was adequate for this study, we used a priori power analysis using the G*Power 3.1.9.2 [In the first visit, participants’ height and body mass were measured using a digital stationary stadiometer SECA 264 (Hamburg, Germany) and a calibrated digital floor scale SECA 874 (Hamburg, Germany), respectively.The Human Ethics Research Committee approved this study of the Sport Sciences Research Institute of Iran according to the compliance with the Ethical Standards in Research of the Ministry of Science, Research and Technology, with the code IR.SSRI.REC.1400.1276 (date of registration: 23/11/2021), as well as operating in accordance with the Declaration of Helsinki. Before participating in the study, CrossFit participants signed informed consent.