Analysis of Th17/Treg by Flow cytometry
Isolated PBMC from RAD-ON02 patients (see 2.2) were used for the analysis of Th17 and Treg cell populations as previously described (Gating strategy of Treg and Th17 cells. In the SSC/FSC dot plot, lymphocytes were determined. After exclusion of doublets, T helper cells (Th cells) (CD4