Multiplex protein immunoassay
tumor
TUMOR
Plasma (cycle 1, day 1 and cycle 3, day 1) and urine (at time of restaging) were analyzed using the Olink Immuno-Oncology panel, which measures 92 proteins involved in immune response and tumor biology, using the Olink multiplex assay (Olink Bioscience) according to the manufacturer’s instructions. The Olink panel uses proximity extension assay technology, which relies on pairs of DNA-labeled antibodies that bind to target proteins and generate unique reporter molecules that can be quantified by real-time polymerase chain reaction. The Olink panel provides normalized protein expression units (NPX), which are log