Subject selection
cognitive impairment
GASTROESOPHAGEAL REFLUX, SHORT BOWEL SYNDROME
Five patients diagnosed with cognitive impairment were recruited from the Affiliated Hospital of Guangdong Medical University. Inclusion criteria were (1) patients aged ≥ 50 years, (2) patients who met the diagnostic criteria for cognitive impairment, and (3) patients who signed written informed consent for this study. Exclusion criteria comprised (1) a history of short bowel syndrome and gastroesophageal reflux, (2) a history of recurrent aspiration, (3) low immunity, (4) disagreement to sign the informed consent, and (4) a history of taking probiotics and antibiotics within 1 month. No dietary changes or additional recommendations were made after FMT or during sample collection.FMT capsules were produced by Xiamen Treatgut Biotechnology Co. Ltd. The donors were healthy college students around 20 years old, and screened through a series of questionnaires, clinical tests and metagenomic sequencing. Previous study have shown that Treatgut Biotechnology company (Xiamen,China) has recommended more stringent donor screening criteria for FMT in China (