Quantitative reverse transcription PCR(RT-qPCR) and immunohistochemical analysis
In this study, the researchers acquired the hTERT-HPNE human pancreatic duct epithelial cell line along with the PANC-1 and AsPC-1 human PCa cell lines from the esteemed Chinese Academy of Sciences type culture collection. Total cellular RNA was extracted according to the operating details of the RNAsimple Total RNA Kit (Tiangen Biotech). Total RNA was reverse transcribed to cDNA using the FastKing RT kit (Tiangen Biotech). SuperReal PreMix Plus (SYBR Green) was added in a real-time quantitative polymerase chain reaction. Primer sequences used in the assay are shown in Supplementary Table 