FMT regulated serum metabolome.
A total of 512 cation peaks, 271 anion peaks, 386 positive metabolites, and 222 negative metabolites were detected in serum before and after FMT by LC/MS mass spectrometry.OPLS-DA could better identify the differences between groups and improve the effectiveness and analytical ability of the model. As shown in Heatmap of serum metabolomics correlation before and after FMT. The volcano map illustrated the up-and down-regulated serum metabolites before and after FMT, with each dot representing a specific metabolite and the size of the dot indicating the VIP value. After FMT, 7 metabolites were up-regulated and 28 metabolites were down-regulated (Volcano map of serum metabolomics before and after FMT. VIP analysis showed the expression patterns of different metabolites in each sample, Comparison of serum metabolites before and after FMT. Box charts showed statistical difference in the 4-Hydroxypheoxyacetate VIP analysis and HMDB compound classification of serum metabolomics before and after FMT. The differences in metabolites before and after FMT were classified into compounds. 