Translational outcomes
MSS, cancer, tumor, tumors
CANCER, TUMOR, TUMORS
While higher baseline ALP levels correlated with higher tissue expression of Tregs (TCR sequencing of blood samples revealed that the median number of novel and > fivefold expanded clonotypes was 35.5 (IQR 27.5–63.5) after two weeks, compared to 70.5 (IQR 37.0–125.25) six weeks after CryoIT (Fig. While TCR clonotypes found in pre-treatment tumor biopsies could be identified among the clones detected in blood sampled pre- and post-treatment, less than 1% of the newly identified or expanded clonotypes was detected in the biopsy samples (Supplementary Table S8).Both the custom 360 gene and the TSO500 gene panels identified the tumors as MSS with low TMB, and immunohistochemical analyses of MSI status by MMR proteins confirmed MSS results. Most biopsies demonstrated low tumor cell fractions (< 20% cancer cells) (Fig. Results of the genetic and protein expression analyses of the tumor tissues. 