2.3. Human Blood Polymorphonuclear Neutrophil (PMN) Purification
BLOOD, CROSS
Human blood polymorphonuclear neutrophils were isolated from buffy coat samples (Bavarian Red Cross Blood Bank, or Sonnen-Gesundheitszentrum, Dr. Gerd Becker, Munich, Germany), or immediately isolated from the samples coming out of the intervention study (9 mL EDTA tubes), and purified using Ficoll density gradient centrifugation to at least 90% purity as confirmed by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany), as described previously [For Ca