Isolation and ex vivo culture of auto-BM-MNC
diabetic
STERILE
Bone marrow was obtained after informed consent from diabetic patients with CTLI and was aseptically stored in sterile Hank's Balanced Salt Solution (HBSS) (Gibco, Grand Island, NY, USA) supplemented with heparin (Fresenius Kabi, Chile). Briefly, mononuclear cells were separated by centrifugation in a Ficoll-Hypaque gradient (density 1.077 g/cm