MDSC Flow Panel
Peripheral blood mononuclear cells (PBMC) were collected pretreatment, cycle 1 day 8, and day 1 of each subsequent 28-day cycle and cryopreserved until needed. Once thawed, PBMC samples were stained with a fixable Aqua viability dye (Invitrogen) and a cocktail of antibodies to the following surface markers: CD14-PerCP-Cy5.5 (BD Biosciences, M5E2), HLA-DR-ECD (Beckman Coulter, Immu-357), Lineage cocktail – CD3/CD16/CD19/CD20/CD56-FITC (BD Biosciences, SK7/3G8/SJ2 5C1/L27/NC AM16.2). Stained cells were acquired on a BD Biosciences LSRFortessa and analyzed using FlowJo software (FlowJo, LLC). CD14