2.6. Blood Samples for Determining Cardiometabolic Risk Biomarkers
BLOOD, INSULIN RESISTANCE
Blood samples collected from the antecubital vein were taken in a fasted state. All of them were centrifuged (i.e., 4000 rpm during 10 min at 4 °C) and frozen (i.e., at −80 °C). Cardiometabolic health parameters were assessed using routine methods (i.e., spectrophotometry, chemiluminescence assay and enzyme-linked immunosorbent assay) including glycemic profile (i.e., glucose and insulin), lipid profile (i.e., total cholesterol, high-density lipoprotein cholesterol (HDL-C), triglycerides (TGs)), liver profile (i.e., glutamic oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), γ-glutamyl transferase (γ-GT)) and inflammatory profile (i.e., C reactive protein (CRP) and leptin). We subsequently calculated HOMA-IR (the homeostatic model assessment of insulin resistance index) [as insulin × glucose/22.5] [