3D-DIA proteomics assays
3D-DIA
Prior to the start of the first cycle of chemotherapy and at the end of treatment, peripheral blood samples of consented patients were taken for 3D-DIA proteomics assays by Jingjie PTM BioLab (Hangzhou, China) Co. Ltd. The standard operational protocols were used for protein extraction, trypsin digestion, HPLC fractionation, and LC‒MS/MS analysis. (Details in supplementary Materials and Methods).