2.3.1. Main Outcome
fatigue
SECONDARY
GH responses to physical acute stress (GH 20’): GH is usually stimulated by the stress of vigorous exercise. To evaluate the response of the GH to acute exercise, and after obtaining a basal blood sample, participants carried out the same vigorous physical exercise for 15 min, at sufficient intensity for fatigue to appear. Specifically, since the blood extraction was carried out in a hospital and the sample was taken immediately after the exercise, a secondary staircase of the hospital, close to the extraction room, was used for the participants to go up and down. A slow but constant rhythm of going up and down stairs was used for 15 min. The rhythm was marked by a person from the team, so that 30 steps were going up and down at a regular pace, with the help of the handrail, using between 1.45 and 2 min, and repeating the same action until completing a total of 15 min. The blood sample was taken after waiting for 5 to 10 min.