Sequence effect worsens over time

Sequence effect worsens over time off therapy. Average slope of change over time (thick black line) with individual data overlaid as line plots (light gray) of sequence effect. * indicates significant change over time. Dashed lines represent the 95% confidence interval of the slope estimate.The sequence effect significantly increased over time, off therapy (β= 0.0453 (95% CI: 0.0328 to 0.0578), 