HPLC analysis of the phenolic/polyphenolic compounds of the aqueous and ethanolic extracts of 
100 mg of both extracts were dissolved in 5 ml of ethanol–water at a ratio of 80:20 v/v, then the mixtures were ultrasonicated at 25 °C for 25 min at 60% duty cycles. After this step, the mixtures were centrifuged at 7500 rpm for 15 min. The supernatant of the extracts was treated with charcoal to remove pigments removal and then evaporated in vacuo. The dried extracts were mixed with 1 ml of HPLC methanol by shaking, and the suspensions were passed through a 2.5 μm filter and stored at 4 °C until analysis. The extracts were injected into the HPLC system under optimal conditions. HPLC was performed using a Shimadzu 10 AV-LC, and peaks were detected and monitored using a UV–Vis 10AV-SPD spectrophotometer (Shimadzu, Kyoto, Japan). In addition, peaks found in the extracts were detected by comparison with the suspensions of the standard materials.