4.8.11. Vitamin C Levels
Briefly, 100 µL of 20% trichloroacetic acid were added to 100 µL of plasma and centrifuged at 5000 rpm for 5 min. Then, 200 µL of Folin–Ciocalteu reagent 0.20 mM was added to the supernatant. The mixture was incubated for 10 min. The absorbance was measured at 760 nm [