2.4. Virtual screening
For virtual screening library of FDA-approved drugs containing 5540 compounds was used. Using the screen library protocol of DS, this library of compounds was screened against the best-validated pharmacophore model of each PPAR. The optimizing features parameters were set as 3 for minimum and 4 for maximum. The three feature best hits and all four features’ hits resulted from the virtual screening against their respective pharmacophore was docked to their relative subtype receptor.