Bulk RNA sequencing and analysis in organoids
ERAS007
Organoids were treated with or without dabrafenib (100 nM) + trametinib (10 nM) or dabrafenib (100 nM) + ERAS007 (100 nM) for 72 h and subjected to RNA extraction using the RNeasy Kit (Qiagen). A strand-specific transcriptome library was constructed and sequenced at BGI Genomics using the DNBSEQ platform. Paired-end 100-base pair RNAseq, 20 million reads per sample, were mapped to the reference genome (GCF_000001405.38_GRCh38.p12) using HISAT. Bowtie2 was used to align the clean reads to the reference genes. GO enrichment analysis was performed using the GO enrichment analysis web-based platform (