Further investigations
PROLIFERATION
Human leukocyte antigen antibody detection (Luminex, ELISA and CDC) and crossmatch techniques (ELISA and CDC), lymphocyte proliferation assay (mixed lymphocyte culture), determination of lymphocyte subsets in peripheral blood (flow cytometry), and determination of cytokine and chemokine levels in plasma (Luminex Performance Assays, R&D Systems, Wiesbaden, Germany) were performed as previously described (