Bio-layer interferometry (BLI)
AHC
An Octet RED instrument (FortéBio) instrument was used to measure recognition of isolated antibodies to HIV-1 BG505 DS-SOSIP, glycan-base BG505, ConC and glycan-base BG505-N241 trimers. Isolated antibodies (50 μg/mL) were immobilized for 300 s on AHC biosensor tips and equilibrated for 60 s in Octet Kinetics Buffer (Sartorius Corporation) prior to measuring association with HIV-1 trimer diluted in Octet Kinetics Buffer (100 μg/mL). The association of HIV-1 trimers were recorded for 300 s. All BLI experiments were conducted at 30°C and were performed in duplicate. Parallel correction to subtract systematic baseline drift was carried out by subtracting the measurements recorded for a loaded sensor incubated in Octet Kinetics Buffer. Response levels of sensors were analyzed using Octet and GraphPad Prism 9 software.