Supplementary Material
cancer, tumor
CANCER, TUMOR, TRIPLE-NEGATIVE BREAST CANCER
Supplementary Movie 1Click here for additional data file.Expression dynamics and cellular localization of ICOS.Click here for additional data file.Supplementary MethodsClick here for additional data file.Exemplar flow cytometry gating strategy.Click here for additional data file.Flow cytometry gating strategy for ICOS on individual T cell populations.Click here for additional data file.Affinity and kineticsClick here for additional data file.Uncropped gel imagesClick here for additional data file.Feladilimab induces phospho-AKT in ICOS-expressing Ba/F3 cells and cytokine production in PBMC from healthy donors and cancer patients.Click here for additional data file.Feladilimab-mediated ICOS co-stimulation results in differential phenotypic changes and cytokine production in CD4+ non-Treg and Treg populations.Click here for additional data file.Healthy human donor CD4+ T cells were pre-activated with antiCD3/CD28 for 48 hours and then re-stimulated with plate-bound anti-CD3 in the presence of increasing concentrations of soluble or plate-bound feladilimab or isotype control for 72 hours. IFNg levels were then assessed from cell-free supernatants using Meso Scale Discovery (MSD)-based detection.Click here for additional data file.Pharmacokinetic analysis of the mIgG1 anti-ICOS antibody (7E.17G9) in the peripheral blood of EMT6 tumor-bearing mice.Click here for additional data file.Antitumor activity of feladilimab in a humanized patient-derived xenograft model of triple-negative breast cancer.Click here for additional data file.Rationale for combination of feladilimab with anti–PD-1 blockade.Click here for additional data file.Clinical case study of feladilimab monotherapy.Click here for additional data file.Clinical case studies of feladilimab monotherapy and in combination with pembrolizumab.Click here for additional data file.Antibodies used for in vitro and in vivo assays, and experimental modelClick here for additional data file.Table S2Click here for additional data file.Gene expression in human T-cellClick here for additional data file.Table S4Click here for additional data file.Pearson correlation (r) analysis of ICOS versus PD-L1 (CD274) in different tumor type.Click here for additional data file.Example TIL marker expression phenotypes from clinical patient case studies of feladilimab monotherapy and in combination with pembrolizumab.Click here for additional data file.