Participants
Infertility, infertility
ENDOCRINOPATHIES, ENDOMETRIOSIS, INFERTILITY
Eligible participants were couples undergoing ART, with indication of tubal and
ovulatory factors, as well as infertility factors without apparent cause, who
were aged <35 years, with at least six oocytes recovered after ovarian
stimulation, and who agreed to sign the informed consent form (ICF). Patients
with endocrinopathies, endometriosis, male factor, and those who did not agree
with the ICF were excluded.The study was carried out at the Reproductive Medicine Service of the Hospital
Escola Álvaro Alvin/Center for Infertility and Fetal Medicine of Northern
Fluminense, located in the city of Campos dos Goytacazes, Rio de Janeiro,
Brazil. It occurred in the period between March 2017 and July 2018.The time of conducting the research was associated with the time of the master’s
course, with 8 months defined for training and analysis of the data for each
group. The first group was decisive for the sample size. During the total period
of this pilot study, 160 cycles of ART were performed, the first group formed
was Group D5, with embryonic culture for five days. Nine participants were
selected, and of these eight were eligible. After the end of the defined period
for Group D5, Group D3, with culture for three days, began. In D3, 13
participants were selected and eight were eligible. Participants who were not
allocated to groups, one for Group D5 and five for Group D3 did not reach the
minimum number of oocytes needed (6 oocytes) to participate in the study.The number of six oocytes was established in order to have a balanced
randomization between the evaluated IVC and IVF techniques. Randomization of
oocytes was performed by drawing a lot immediately after follicular aspiration,
even before oocyte classification. Three oocytes were used in the IVC technique
with the INVOCell™ device and three oocytes in the IVF technique in an
incubator. Oocytes considered surplus, and those which exceeded the required
six, were submitted to the ICSI fertilization technique (
Flow Diagram and follow-up study. 160 cycles of ART were performed,
nine participants were selected, and of these eight were eligible
and formed Group D5 and thirteen participants were selected and
eight were eligible and formed Group D3.