Tumor microenvironment and immune invasion analysis
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INFILTRATION
The stromal score, immune score, and ESTIMATE score of each patient were calculated using the ESTIMATE algorithm with R packages “limma” and “estimate.” We employed the R packages ‘reshape2’ and ‘ggpubr to examine the aforementioned scores among the high and low risk groups. Subsequently, we generated a violin plot to investigate potential disparities among the three patients in the high and low risk groups. Moreover, the examination of immune cell infiltration in each individual was conducted utilizing the R software package called “CIBERSORT,” and the outcomes were presented visually with the aid of the R packages “reshape2” and “ggpubr.” Furthermore, we conducted examination to assess immune-related functions and generated box plots to display the outcomes, utilizing the R packages “limma,” “GSVA,” and “GSEABase.”