Culture
After exposure, the specimens were placed in small to medium-sized zip lock bags and washed with 50 ml of PBS. The suspension was placed in a shaking incubator for 1 min. This allowed the suspension of the microorganism in the solution. Next, an amount of 20 ml of the PBS of each sample was aseptically plated into Mueller Hinton agar media by pouring plate method for the observation and counting of colonies. The media plates were incubated at 37 °C for 24 h.Colony Forming Units (CFUs) were counted for each of the four materials, and compared among the three groups (one-way ANOVA, and a post-hoc Tukey test, p = 0.05) using the GraphPad software.