Biomarker analyses
tumor necrosis
INFLAMMATION, ADHESION, TUMOR NECROSIS
Serum samples for biomarker analyses were analyzed using qualified antibody-based assays. Serum concentrations of the T helper 17 (Th17) effector cytokine IL-17A were analyzed using Simoa™ single molecule array technology (Quanterix Corp., Billerica, MA), and those of IL-17F and IL-22 were analyzed using the Single Molecule Counting SMCxPRO Immunoassay Platform (Millipore, Burlington, MA). Acute phase proteins and markers of inflammation, CRP, serum amyloid A (SAA), IL-6, IL-10, interferon γ (IFNγ), tumor necrosis factor α (TNFα), soluble intercellular adhesion molecule-1 (sICAM), and vascular cell adhesion molecule (sVCAM), were analyzed using the Meso Scale Discovery Platform (Meso Scale Diagnostics, Rockville, MD).