References
TNF-α, tumor necrosis
REGRESSION, INSULIN RESISTANCE, TUMOR NECROSIS
Flow chart of the participants who started and completed the intervention.Flow chart for the design of the prediction model based on DNA methylation.Flow chart for the selection of methylation sites (CpG) used for the prediction model.Prediction model of the percentage of BMI loss according to the total methylation score of each individual. “Y” axis: percentage of BMI loss and “X” axis: methylation score. According to the total score, it can be predicted with which diet the greatest percentage of BMI is achieved for each individual.Representation of the distribution of the Representation of the population distribution with respect to the total methylation score. “X” axis shows the total methylation score. “Y” axis shows the number of participants.Biological role of genes that have functions in metabolism, the immune system, and cell function and that contain the CpG sites selected for the MHP diet prediction model.Biological role of genes that have functions in metabolism, the immune system, and cell function and that contain the CpG sites selected for the LF diet prediction model.Baseline anthropometric and biochemical data from the groups with a moderately high-protein (MHP) and a low-fat (LF) diet.Values correspond to the mean ± SEM. BMI: body mass index, HOMA-IR: insulin resistance index. HDL: high-density lipoprotein, LDL: low-density lipoprotein, ox-LDL: oxidized low-density lipoprotein, Alt: alanine aminotransferase, Ast: aspartate aminotransferase, and TNF-α: tumor necrosis factor alpha. Anthropometric and biochemical changes that occurred with the interventions with each of the two diets (MHP and LF) after 4 months and the differences between them.Values correspond to the baseline mean and mean changes after caloric restriction treatment ± SEM. BMI: body mass index. HOMA-IR: insulin resistance index. HDL: high-density lipoprotein, LDL: low-density lipoprotein, ox-LDL: oxidized low-density lipoprotein, and Alt: alanine aminotransferase. Ast: aspartate aminotransferase. TNF-α: tumor necrosis factor alpha. Spearman’s correlations between methylation of the significant CpG sites (Spearman’s correlations between methylation of the significant CpG sites and the percentage of BMI loss of the 201 subjects. Spearman’s correlations between methylation of the significant CpG sites (Spearman’s correlation of CpG sites with the percentage of BMI loss. Multiple linear regression for MHP diet prediction model showing an association between methylation of the CpG sites and BMI difference (Multiple linear regression model using the percentage of BMI loss as the dependent variable and the CpG sites for the MHP diet as independent variables. SEM: standard error. Beta values represent changes in results for the increasing number of BMI percent loss units.Multiple linear regression for the LF diet prediction model showing an association between methylation of the CpG sites and BMI difference (Linear regression model using the percentage of BMI loss as the dependent variable and the CpG sites for the LF diet as independent variables. SEM: standard error. Beta values represent changes in results for the increasing number of BMI percent loss units.Design of weighted sub-scores for the diet MHP, LF, and total score.Linear mixed effect model for the prediction of the percentage of BMI loss.Dependent variable percentage of BMI loss, fixed effect variable diet, total score, and interaction of the diet and total score. Adjusted for age and sex. Diet## total score indicates the interaction between both variables. SEM: standard error.Information from the 15 methylation sites associated with the MHP diet selected for the prediction model.Information from methylation sites of the MHP diet prediction model by using the “Illumina MethylationEPIC array”. The location on the chromosome, the related gene, and the region in which each CpG is found were analyzed for each methylation site. Gene Information from the 11 methylation sites associated with the LF diet selected for the prediction model.Information from the methylation sites of the LF diet prediction model by using the “Illumina MethylationEPIC array”. The location on the chromosome, the related gene, and the region in which each CpG is found was analyzed for each methylation site. Gene 