Introduction
infection, HSV2
ADVERSE REACTIONS, INFECTION, DISEASE
Systems vaccinology aims to improve understanding of vaccine outcomes, by using unbiased approaches to identify the major associated immune phenotypes, including peripheral blood cell population frequencies and gene expression (Sex is a well-known variable that can influence vaccine and disease responses. Females typically develop higher antibody responses and report more adverse reactions following vaccination than males (Here, we analyzed a unique cohort receiving a multidose, replication-defective vaccine, to interrogate the interaction of sex and prior exposure to infection. Subjects received an experimental HSV2 vaccine in a phase 1 clinical trial where comparisons could be made between three groups of volunteers based on their HSV serostatus prior to vaccination: HSV1We characterized immune responses longitudinally by analyzing blood transcriptomic profiles and high-dimensional immune cell phenotyping throughout the three-dose vaccine regimen (