Drug sensitivity testing in patient-derived organoids
PROLIFERATION
Drug sensitivity testing was performed in TDOs generated from metastatic colorectal biopsies using the IndiTreat® platform from 2cureX laboratories (Cell growth was measured by taking brightfield images on day zero and visualizing living cells on day seven using a fluorescent stain (CyQUANT Cell Proliferation Assay, ThermoFisher Scientific Inc.). Images from days zero and seven were analyzed using the artificial intelligence image analysis algorithm IndiNet™. IndiNet™ quantifies the area of TDOs in both brightfield (day zero) and fluorescence (day seven) images and calculates the relative drug induced growth inhibition by comparing treated with untreated TDOs from the same patient (Supplementary Figure 