Plasma soluble BDNF, Corin, BNP and NEP levels tests
SECONDARY
Plasma-soluble brain-derived neurotrophic factor (BDNF) level was measured using a human BDNF ELISA kit (Catalog: JL11683, Jianglai Inc., Shanghai, China); plasma-soluble Corin level was measured using a human Corin ELISA kit (Catalog: IC-Corin-Hu, IC ImmunoClone Inc., Shanghai, China); plasma-soluble NEP was measured using a human NEP ELISA kit (Catalog: JL15469, Jianglai Inc., Shanghai, China); clinical data were used to determine the plasma-soluble BNP level. Among them, our primary outcome was a change in plasma BNP levels before and after administration of sacubitril/valsartan. While other measures, including plasma levels of BDNF, Corin, and NEP before and after medication, were secondary outcomes of our analysis.