Faeces
5-day full faecal samples were collected by participants on the last 5 days of each study period. Participants were provided with airtight sample collection pots (Covidien Commode Specimen Collector, REF2400SA) and were instructed to collect all faecal mass produced over the 5-day period. Samples were kept cool and brought to the UCD laboratory within 24 h of being produced, where they were weighed and stored at − 20. All samples received were freeze-dried, and each 5-day sample (or 4 day and 3 day in some cases) for each participant was pooled and stored at room temperature. Not all seven completers provided full samples on 5 consecutive days. Of the 7 completers, 4 people provided full 5-day samples (12 occasions), while the other volunteers missed a morning or evening sample on 1 or 2 occasions. When this happened, the largest number of consecutive days’ sample was taken and pooled. On three occasions, a pooled 3-day consecutive sample was used, and on five occasions, a pooled 4-day sample was taken. In summary, 13 of the potential 21 5-day pooled samples were achieved for a 5-day full consecutive sample, 5 were for a 4-day sample and 3 were for a 3-day pooled sample. Faecal fat analysis, (and faecal fatty acid profiling) was conducted at the University of Aarhus Denmark, using a modified Bligh and Dyer technique for total fat [