Procedures
tumor
TUMOR, CAVITY, TUMOR RECURRENCE
Prior to study intervention and tumor recurrence, all patients had received standard treatment for newly diagnosed GBM, which was surgery followed by standard Stupp protocol, including radiation therapy with a total dose of 60 Gy given in 2 Gy daily fractions over a period of 6 weeks and chemotherapy with temozolomide 75 mg/mThe study intervention mainly consisted of two steps, including direct injection of HSV-carrying ADSCs into the tumor and prodrug administration. The ADSC administration was performed using frameless stereotaxy and neuronavigation system. Following the localization of the tumor by preoperative stereotactic MR imaging, intracerebral stereotactic injection to the cavity was performed under general anesthesia. After the needle reached the central portion of the tumor, through using a guiding cannula, 1 mL of ADSC-containing suspension was manually injected over 1 min. The needle was kept in place for two minutes after the injection. Given the study design, it included three cohorts of patients, and based on the cohort number, different numbers of HSV-TK-carrying ADSCs were administered. Cohort 1 (n = 3) received 2.5 × 10