Conclusion
tularemia
TULAREMIA
In this study, our goal was to establish parameters and guidelines for RNA-Seq studies using patient PBMC samples obtained before and after vaccination with a live attenuated tularemia vaccine. Estimating statistical power in RNA-Seq studies is a complex endeavor, as power is strongly influenced by the biological variance of the particular experiment, the range of effect sizes, and the technical factors we have described in this study. The near-universal usage of Illumina sequencing has partially standardized the technical factors influencing RNA-Seq power. A number of recent studies have established a framework of statistical power and quality control for RNA-Seq experiments, and their findings are largely in agreement with our data (