Introduction
non-small cell lung cancer, tumor, cancer, Lung cancer, intraoperative adverse reactions, lung cancer
NON-SMALL CELL LUNG CANCER, TUMOR, INFLAMMATORY RESPONSES, CANCER, LUNG CANCER, ADVERSE EFFECT, LUNG CANCER, TUMORIGENESIS
Lung cancer is the leading cause of cancer-associated mortality worldwide [Several in vivo studies have investigated the effects of propofol and sevoflurane on cancer. For example, among patients who received propofol during lung cancer resection, their perioperative inflammatory responses and rates of intraoperative adverse reactions were significantly reduced compared with those who received sevoflurane [Researchers also evaluated the effects of propofol and sevoflurane on lung cancer in vitro. It has been demonstrated that propofol has an adverse effect on cell viability and promotes apoptosis by downregulating the miR-21-5p/MAPK10 axis in the non-small cell lung cancer cell lines A549 and H1299 [Overall, the effects of propofol and sevoflurane on patients who undergo lung cancer surgery are still unclear. More importantly, the underlying molecular mechanism remains unexplored. In this research, we conducted an in vivo study, and eight lung cancer patients with tumor and paired normal samples were collected, among which 4 patients received propofol and 4 patients received sevoflurane. We used RNA-seq to obtain a comprehensive profile of the differentially expressed genes (DEG), aiming to decipher the key factors of propofol and sevoflurane on lung cancer. Finally, selected candidate genes were confirmed by qRT–PCR. This study sheds light on the effects of anesthesia at the whole transcriptome level and explores the possible genes and pathways involved in tumorigenesis, which provides a basis for future work.