RNA extraction
Samples (snap-frozen white blood cell pellets) were received on dry ice and stored at −80°C until processing commenced. Samples were randomized before extraction. In total, samples from 15 subjects (5 from the placebo group, 2 from 0.005 mL/kg MRG-001, 4 from 0.01 mL/kg MRG-001 and 4 from the 0.02 mL/kg MRG-001 dose group) were sequenced. The Lexogen 008 split RNA extraction kit was used to extract RNA. Samples were characterized by UV-Vis spectrophotometry (Nanodrop2000c, Thermo Fisher), the RNA integrity was assessed on a Fragment Analyzer System using the DNF-471 RNA Kit (15nt) (Agilent).