RNA purification & next generation sequencing
To understand the molecular mechanisms of MRG-001, next-generation sequencing on RNA isolated from PBMCs was performed and gene expression changes were quantified. Sequencing occurred at multiple time points after injection to define the dynamics of gene expression changes between the different dose groups. Gene expression changes were pooled per timepoint per dose MRG group and compared to the pooled pre-dose gene expression of that specific dose group and to the placebo-treated group.