2.2. DNA Extraction and Sequencing
STERILE
Fecal samples were collected by spatula into 60 mL sterile fecal collection containers and placed in the laboratory freezer (−20 °C) within 15 min of production. Thereafter, samples were homogenized, aliquoted and stored at −80 °C in 1.5 mL tubes (Qiagen, Germany) for later analysis of Internal Transcribed Spacer 2 (ITS2) sequencing [The fungal ITS rRNA gene was amplified by performing real-time PCR (ABI GeneAmp