The preparation of ADSCs
The drug in this trial was a solution of ADSCs. Adipose tissues were harvested with liposuction of abdomen from a healthy donor. They are washed in phosphate-buffered saline (PBS) and digested of fat aspirates with 0.075% collagenase. These stem cells were then packed in dimethyl sulfoxide and human platelet lysate and 1% antibiotics at a culture condition of 37 °C and 5% CO