Diet intervention and physical activity
This was a free-living study and no food was provided to the participants. The LCD group was instructed to follow a diet consisting of a maximum of 20 E% carbohydrates, 50–60 E% fats 25–30 E% protein with a recommendation of a high intake monounsaturated fatty acids (MUFAs) and as low intake of saturated fatty acids (SFA) as possible [All participants were individually introduced to the diet by a licensed clinical dietitian, had opportunity for on-demand visits and could attend group-specific discussion meetings supervised by the dietitian. The dietitian contacted every participant per telephone one week after starting the new diet and hereafter every month. A five-days startup menu plan based on pre-study calorie intake was provided as well as weekly newsletters to all participants. Participants had access to a recipe-database that was continuously updated and were instructed to register food intake in MadLog (MadLog ApS, Kolding, Denmark). Based on the estimated energy requirement at baseline, participants were guided to maintain their calorie-intake during the entire intervention. Furthermore, the participants were instructed maintain their usual physical activity level throughout the study period. As reported [