Ethics approval and consent to participate
All of the performed procedures were conducted in accordance with the ethical principles and standards of the institutional and national research committee and with the Declaration of Helsinki and its later amendments (revised in October 2018). The study protocol protocol was independently reviewed and approved by the Institutional Ethical Committee, Faculty of Dentistry, Ain shams University (FDASU-RecD061808). Informed written consent was obtained from all individual participants included in the study.