Fecal and circulating bile acids
SECONDARY
All major primary (e.g., CA and CDCA) and secondary fecal and circulating BAs (e.g., DCA and LCA) as well as their glycine and taurine conjugates will be assessed. Total BAs and total unconjugated BAs will also be measured. Samples will be extracted, and supernatants will be dried and resuspended for liquid chromatography/mass spectroscopy analysis following validated and published methods [