Consort diagram for the CALERIE Trial.
BLOOD
DNAm was assayed from blood samples collected at baseline, 12 months and 24 months. Of the Blood DNAm data were generated at baseline and at least one follow-up timepoint for Characteristics of CALERIE Trial participants at baselineThe table shows data for participants randomized to the AL control group and the CR treatment group. CALERIE included a total of The goal of our analysis was to test the effect of CALERIE intervention on biological aging. We measured biological aging from blood DNAm using published algorithms. These algorithms aim to capture the accumulation of molecular changes that underlie the progressive loss of system integrity that occurs with advancing chronological age. Primary analysis focused on the PhenoAgeDNAm clock and pace-of-aging measures included in CALERIE analysisWe computed change scores for the DNAm measures of aging as the differences of 12-month and 24-month follow-up values from baseline values. For analysis, change scores were scaled so that effect sizes can be interpreted as standardized differences between means (Cohen’s 