Characteristics of 16S Pacbio sequencing results
The effects of W-LHIT on the intestinal microbiota composition were assessed by sequencing the bacterial 16S rRNA. A total of 417,913 optimization-CCS sequences and 352 OTUs (97% similarity) were obtained from the 74 samples through a single molecules real-time sequencing analysis, with an average of 5,647 reads and 82 OTUs per sample (
The alpha diversity indexes, including Ace and Chao indices, rarefaction, Shannon-index (