Discussion
anxiety, insomnia, depression, anxiety-related mental disorders, cancers, gastrointestinal disorders
COMPLICATIONS
The academic examination is the primary source of brief naturalistic stress for college students, which has been used frequently as a stress model to investigate the psychological stress responses together with gut microbiota changes and to evaluate the influences of probiotics or prebiotics on stress-induced complications (The anxiety, depression, and insomnia levels were measured by the most commonly used mental health questionnaires, such as HAMA-14, HDRS-17, and AIS-8 (Considering evidence suggested that probiotic interventions could balance microbial populations thereby create healthy intestinal homeostasis to support the life system. There is an assumption that a higher gut microbiota diversity generally means a better gut environment, which has been proved by several conditions (e.g., cancers, gastrointestinal disorders, and stress-related conditions) accompanied by a reduced gut microbial diversity (Our results showed that the content of Furthermore, gut microbial metabolic actions or gut microorganism-originated metabolites are the major pathways promoting the communication between gut and brain Among them, 13 variables formed a subset related to probiotic JYLP-326 treatment (Although the potential role of JYLP-326 in remodeling the disturbed gut microbiota has been illustrated in this study, it’s still unclear whether this efficacy was caused by probiotic administration or individual differences among subjects. It would be better to take into account the impact of gut microbiome heterogeneity on the results of gut microbiota and its metabolism by collecting fecal samples before the treatments as additional control groups for further comparative analysis. Moreover, the underlying mechanisms of JYLP-326 in relieving the test anxiety-related mental disorders also should be explored deeply by using anxious/depression animal models and advanced molecular techniques, particularly in how to link the microbiota-gut-brain axis with the biological functions of probiotic JYLP-326 in the future, a critical issue on the applying of probiotics in the clinic.