Analysis of p53-Immunoreactive Subgroup
death
IHC indicates immunohistochemistry; p53-Ab, anti-p53 antibody. Nelson-Aalen cumulative hazard curves are presented in the p53-Ab (+) and p53-IHC (3+) subgroup, p53-Ab (−) or p53-IHC (2+, 1+, or 0) subgroup, p53-Ab (+) and p53-IHC-positive subgroup, and p53-Ab (−) or p53-IHC-negative subgroup. Hazard curves for relapse or death in patients taking vitamin D were compared with those taking placebo.To conduct a sensitivity analysis using a cutoff p53-IHC value of 10% instead of 99%, patients were divided into 2 groups of p53-IHC (+), or greater than 10%, and p53-IHC (−), or 10% or less. In the p53-Ab (+) and p53-IHC (+) group, relapse or death occurred in 10 of 59 patients (16.9%) in the vitamin D group and 17 of 34 patients (50.0%) in the placebo group; the 5-year RFS was significantly higher in the vitamin D group (14 patients [80.7%]) than the placebo group (2 patients [37.4%]; HR, 0.31; 95% CI, 0.14-0.67; estimated power > 0.99) (