2.9.1. Plasma Cytokine
TUMOUR NECROSIS
Plasma cytokine concentrations were analysed by bead array using Biolegend Legendplex™ 13-plex Human Essential Response panel (Cat. No. 740930, San Diego, CA, USA) according to the manufacturer’s instructions. The simultaneous quantitation of 13 cytokines (interleukin (IL)-4, IL-2, C-X-C motif chemokine ligand (CXCL)10 (IP-10), IL-1β, tumour necrosis factor (TNF)-α, monocyte chemoattractant protein (MCP)-1, IL-17A, IL-6, IL-10, interferon (IFN)-γ, IL-12p70, CXCL8 (IL-8), and transforming growth factor (TGF)-β1) in plasma samples was measured using a BD FACSverse™ flow cytometer (BD Biosciences, San Jose, CA, USA).