Pharmacokinetic sampling and analytical assay
BLOOD
Samples for assessment of pharmacokinetics were taken during the first cycle only, on day 2 and day 8. Blood samples were obtained from an indwelling canula in EDTA tubes as follows: before infusion, 15, 30 min, 1, 2, 4, 8, 16, 24 h after the end of the intraperitoneal chemotherapy infusion. A peritoneal fluid sample was taken through the Porth-a-Cath system at the end of intraperitoneal drug administration. Immediately after collection both blood and peritoneal fluid samples were centrifuged to separate plasma and debris in peritoneal fluid. An aliquot of plasma and peritoneal fluid was saved and stored at − 40 °C until analysis. For all cisplatin samples, plasma and peritoneal fluid was immediately ultrafiltered through a Centrifree