Neutrophil oxidative burst activity
LYSED
The intracellular oxidative burst capacity of neutrophils was measured using commercially available flow cytometric kit (PHAGOBURST; Glycotope Biotechnology, Heidelberg, Germany). Heparinized whole blood (100 µl) was incubated with either wash buffer (unstimulated control), stimulated with PMA (1.35 µM) or fMLP (0.83 µM) for 10 min at 37 °C followed by incubation with DHR-123 substrate for 10 min at 37 °C. Erythrocytes were lysed with the addition of Lysing Solution (Glycotope Biotechnology). Cells were centrifuged at 250×