Cell intervention and flow cytometry
PBMCs from blood samples collected at 2 years after SLIT were cultured in 1 mL medium (2 × 10Rocky Hill, NJ, USA), TSLP (500 ng/mL, PeproTech, Rocky Hill, NJ, USA), IL-33 (500 ng/mL, R&D, Minneapolis, MN, USA), and IL-25 (500 ng/mL, R&D, Minneapolis, MN, USA) [