2.5. Resting Energy Expenditure
Subjects rested for 10 min in a supine position in a dark, quiet room. Respiratory gases were then collected for 20 min. Data from the first 5 min were excluded from analysis to account for subject adjustment to the protocol. The Weir equation was used to determine rate of kcal/day (Equation (1)). Because nitrogen excretion is minimal in such a short period of time, the nitrogen correction was ignored [Energy derived from total carbohydrate and lipid oxidation was calculated using the following equations (Equation (2)) assuming protein oxidation was close to zero for this brief time period [