Statistical analysis
All statistical analyses were implemented by R 3.6.1 software. There is no prespecific endpoint or criteria for sample size. Medians and quartiles were provided for distributions of time intervals. 95% CIs were constructed using the Clopper-Pearson method for pathological response rates. The Wilcoxon rank-sum test was used to compare TMB, TNB, immune-related gene expression, signature levels, and immune cell estimations between independent groups. The Wilcoxon signed-rank test was used to compare TMB, TNB, immune-related gene expression, signature levels, immune cell estimations, and T cell clone frequencies between matched samples (pre- 