Method of use of the device
LIVER
If the patient is randomized into the machine perfusion group, the first part of graft preservation is similar to the control group. The graft is perfused and stored in Institute George Lopez (IGL-1®) solution at 4 °C during transport to the transplantation center.As soon as the liver graft arrives at the transplantation center, the surgeon immediately prepares it on the back-table. The graft is then put on the Liver Assist perfusion machine around the time the transplant procedure begins.Perfusion settings will be:Use of the same perfusion machine device in all centers (Liver Assist, Xvivo)Hypothermic perfusion (8–12 °C)Portal vein perfusion: portal vein is cannulatedAdjusted perfusion flow: 150–250 ml/min under 3 mmHg in the portal veinPerfusate oxygenation at 70 kPAUse of 3 L of perfusion solution: Machine Perfusion Solution (Belzer-MPS, Bridge-To-Life, CE-certified)