Pharmacokinetics
Lorlatinib was isolated from human plasma by a liquid–iquid extraction procedure. After evaporation of the organic extract under nitrogen, the residue was reconstituted, and the final extract was analyzed for lorlatinib concentration by liquid chromatography with tandem mass spectrometry (LC–MS/MS) using a previously reported assay (Chen et al. PK parameters for lorlatinib were derived by non-compartmental analysis (NCA) using Phoenix WinNonlin version 8.3. A linear-log trapezoidal method was used to calculate AUC. A best-fit method was used to estimate terminal elimination and to extrapolate any plasma concentration versus time profiles to compute AUC