Cardiopulmonary exercise test and resting metabolic rate
We will measure cardiorespiratory fitness by the gold standard cardiorespiratory fitness assessment, an incremental-to-maximum exercise test with open-circuit indirect calorimetry. Monitoring before, during and after the test will include heart rate, pulse oximetry, blood pressure and the Borg rating of perceived exertion. Prior to the exercise test, resting metabolic rate will be assessed by using the same metabolic cart during 10–30 min of supine fasted rest.