Evaluation of periodontal biofilm susceptibility to antibiotics by real-time monitoring of biofilm growth
SEVERE PERIODONTAL DISEASE, PLAQUE
To identify the best individual treatment for each patient with severe periodontal disease, three systemic antibiotics commonly used in dental practice, namely amoxicillin, metronidazole and azithromycin (SIGMA), and the combination of amoxicillin and metronidazole, were tested, by evaluating their effect on biofilm formation of the subgingival plaque samples. Similarly to the experiments described above, the subgingival plaque was collected into 2 mL of VMG III transport media and vortexed for 60 seconds. After that, paper-points were discarded, and samples were centrifuged at 10.000 rpm for 3 minutes. Hereafter, the pellet was resuspended with 2 mL of fresh BHI medium supplemented with VitKTo grow periodontal biofilms in the presence of conventional antibiotics in the RTCA system, 100 µL of each antibiotic diluted in BHI medium supplemented with VitK