Conclusion
This newly developed LC–MS/MS method provides a thorough analytical method for the intended purposes of quantitative determination of the four major parent compounds (andrographolide; 14-deoxy-11, 12-didehydroandrographolide; neoandrographolide; and 14-deoxyandrographolide) in both plasma and urine samples. In this way, this method offers a solution to the limitations inherent in other methods and enables a more complete pharmacokinetic analysis. This highly sensitive method (LLOQ < 1.00 ng/mL), with requiring a short analysis time (6.0 min), was successfully applied in a pharmacokinetic study of orally administered 