3.5. GO enrichment analysis and KEGG pathway enrichment analysis
Human cytomegalovirus infection, cancer, Pancreatic cancer, Kaposi sarcoma-associated herpesvirus infection, Hepatitis B, Hepatitis C
LEGIONELLOSIS, HUMAN PAPILLOMAVIRUS INFECTION, SMALL CELL LUNG CANCER, PROLIFERATION, CANCER, MEASLES, SALMONELLA INFECTION, PANCREATIC CANCER, EPSTEIN-BARR VIRUS INFECTION, BLADDER CANCER, PROSTATE CANCER, ALCOHOLIC LIVER DISEASE, HEPATITIS B, ATHEROSCLEROSIS, HEPATITIS C
The targets were imported into the DAVID data platform for GO annotation analysis using “homo sapiens” as the study context. A total of 210 entries were obtained for GO enrichment analysis, including 159 entries for biological process (BP), 12 entries for cell composition (CC), and 39 entries for molecular function (MF). The top 10 entries were visualized (Q < 0.05) and ranked by gene size. BP was mainly involved in the positive regulation of gene expression, positive regulation of transcription from RNA polymerase II promoter, positive regulation of cell proliferation, positive regulation of transcription, DNA-templated, negative regulation of transcription from RNA polymerase II promoter, response to drug, negative regulation of apoptotic process, regulation of transcription from RNA polymerase II promoter, positive regulation of mitogen-activated protein kinase (MAPK) cascade, and response to estradiol. CC was mainly involved in cytoplasm, nucleoplasm, cytosol, nucleus, chromatin, macromolecular complex, receptor complex, transcription factor complex, perinuclear region of cytoplasm, and death-inducing signaling complex. MF was mainly involved in protein binding, identical protein binding, transcription factor binding, enzyme binding, macromolecular complex binding, transcription factor activity, sequence-specific DNA binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding, RNA polymerase II transcription factor activity, sequence-specific DNA binding, DNA binding, and ubiquitin protein ligase binding. All 3 processes with respective involvement are shown in Figure GO enrichment analysis of key targets. Note: red represents biological processes, blue represents cellular component, and green represents molecular function. GO = gene ontology.98 pathways were obtained by KEGG enrichment analysis through the DAVID data platform, and the top 20 pathways were screened for visualization, which showed that the key targets were mainly involved in Pathways in cancer, phosphatidylinositol-3-kinaseRAC–serine/threonine-protein kinase signaling pathway, Kaposi sarcoma-associated herpesvirus infection, Human cytomegalovirus infection, Measles, Hepatitis C, Hepatitis B, Epstein-Barr virus infection, Proteoglycans in cancer, Lipid and atherosclerosis, MAPK signaling pathway, Prostate cancer, aryl hydrocarbon receptor nuclear translocator signaling pathway, Alcoholic liver disease, Salmonella infection, Human papillomavirus infection, Bladder cancer, Legionellosis, Pancreatic cancer, Small cell lung cancer as shown in Figure Signaling pathways.HIF-1 = aryl hydrocarbon receptor nuclear translocator, MAPK = mitogen-activated protein kinase, PI3K-Akt = phosphatidylinositol-3-kinaseRAC–serine/threonine-protein kinase.KEGG enrichment analysis of key targets Note: the x-axis represents the gene ratio, the y-axis represents the enriched pathways, the size of the dots indicates the gene number, and the color of the dots represents the 