2.2. Subjects
METABOLIC DISEASE, DISEASES, FOOD ALLERGY, DRUG ALLERGY
Healthy Japanese males and females aged 20–60 years who met the inclusion criteria participated in the study. The inclusion criteria were as follows: (1) subjects with a distant bilateral vision of 0.6 or higher (uncorrected or corrected); (2) subjects who routinely played video games, used computers, or performed VDT activities; (3) subjects who could undergo examinations and observations as scheduled; and (4) subjects who received sufficient explanation of the purpose and content of the trial, had the ability to consent, volunteered to participate after understanding the trial fully, and agreed to participate in the trial in writing. The exclusion criteria were as follows: (1) medical history of serious diseases, such as liver, renal, cardiovascular, gastrointestinal, respiratory, hematological, autoimmune, endocrine system, and metabolic disease; (2) history of drug allergy or serious food allergy; (3) continuous use of medicines, health foods, or supplements that may affect the study; and (4) subjects judged by the investigator as inappropriate for the study.