Serum cytokine analysis
Serum protein concentrations were measured by Precision for Medicine using an electrochemiluminescence immunoassay (Meso Scale Discovery). V-Plex Proinflammatory panel 1 was used for the following cytokines: IFNγ, IL-2, IL-6, IL-8, IL-10, IL-12p70, IL-13 and TNF. V-Plex Cytokine panel 1 was used for the following cytokines: GM-CSF, IL-7 and IL-15. Single V-Plex and U-plex assays were used for IL-1RA and IL-2Rα, respectively. Analysis was performed using a MESO QuickPlex SQ 120 instrument and Discovery Workbench 4.0 software (Meso Scale Discovery).