Vaginal microecological detection and bioinformatic analysis
Genomes were extracted from all the vaginal swab specimens using a BIOG DNA Swab Kit (BAIDAI, cat. #51029), according to the manufacturer’s instructions. The PCR of the 16SrRNA V3-V4 region was amplified by a 16S universal primer [forward primer: 5′-CCTAYGGGRBGCASCAG-3′ and reverse primer: 5′-GGACTACNNGGGTATCTAAT-3′]. PCR product purification, adapter ligation, DNA library purification, and quantitative quality control were performed. An Illumina NovaSeq (Illumina Inc., Chicago, IL, USA) was used as the sequencing platform. The effective data quantity was analysed using the rarefaction curve in Rarefaction analysis of V3-V4 MiSeq sequencing reads of the 16S rRNA gene in all samples (n = 78). The Observe, Chao1, and ACE were used in the analysis.Sequences were demultiplexed using Quantitative Insights into Microbial Ecology (QIIME1 version 1.8.0) and operational taxonomic units (OTUs) were generated using the UCLUST (version 1.8.0) denoising single plugin. Deduplication and standard quality filtering procedures were performed using default settings (Bacteria were identified to the genus level using two variable regions of the gene 16s rRNA. A relative abundance bar plot was drawn based on the relative abundance of four 