Isolation of peripheral blood mononuclear cells
PD
LYSIS
PBMC were isolated by density-gradient centrifugation using Lymphoprep™ (Stem Cell Technologies, Vancouver, Canada) followed by erythrocyte lysis (ACK Lysing buffer, Gibco, Waltham, MA, USA). For pharmacodynamic (PD) analysis, cells were resuspended in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), 2 mM 