Hormone parameters
RE52281
The saliva and blood samples were collected immediately at the beginning of T0 (7.30-9.00am). In premenopausal women, saliva and blood samples were collected during the luteal phase (second half of the cycle), when both E2 and P concentrations are high. Hormone concentrations were used only for identification and classification between pre- and post-menopausal states. For saliva samples, specific ELISA kits for E2, P, T, and DHEA concentrations were used (RE52281; RE62141; RE52651; RE30121046; Re62039). Dry blood concentration of FSH was analyzed by an external laboratory according to the CLIA method (Ayumetrix, 17,387 63rd Ave, Lake Oswego, OR 97,035, USA).