Biochemical blood analysis
β-chain
BLOOD, CLOT, SEPARATION
Blood Sampling was conducted in two stages. The first sample was taken one day before the familiarization session with training, and the second one was taken 48 h after the last training session in the eighth week. During both phases and after a 12-hour fast (excluding water), test specimens were obtained from 8:30 until 9:00 a.m. Before each stage of sampling, the participants were in a sitting position resting for a few min; following their rest, 10 mL of blood was obtained from their brachial vein in their elbow area in the shortest possible time. Finally, after blood collection, the samples were placed at room temperature for 20 min to clot, and subsequently, the tubes containing the samples were centrifuged for 12 min at 3,000 revolutions per min (RPM). Then, after separation, the serum was stored at -80 ˚C. Another part was prepared as plasma with the addition of EDTA anticoagulant in order to measure the blood glucose level and HbA1c level. The glycated N-terminal valine of the β-chain is the basis for measuring HbA1c concentration through a particular chemical reaction [