2. Materials and Methods
This study was a randomized self-controlled prospective clinical study. The study was conducted on 24 male patients of American Society of Anesthesiologists (ASA) grades I and II status among those aged of 18 to 50 years and scheduled for major surgeries lasting for more than 4 hours. The Ethical Committee of Post Graduate Medical Institute (PGMI) approved the use of humans in this study vide Human subject application number ANS-6466 dated February 02, 2019 and in accordance with Declaration of Helsinki. Furthermore, as the clinical trials are required registration from an appropriate registry approved by World Health Organization (WHO), this trail also retrospectively registered at Thai Clinical Trials Registry (TCTR), (an approved WHO registry for clinical trials registration) under reference ID Full-length study protocol is provided as supplemental file (Supplemental 