4.3. Limitations
This study has some limitations. First, we directly recruited the video subjects and played relevant videos for them. This test method is insufficient for simulating the situation in which the subjects browse the video on the short video platform. Second, the number of participants was limited, meaning that subgroup analysis of their educational, cultural and economic backgrounds could not be performed. Moreover, this study was a short-term effect study and data from a long-term follow-up survey were lacking. Therefore, for future research on the impact analysis of short video transmission factors, recruiting more participants for subgroup analysis and obtaining long-term intervention data to explore the impact of popular science short videos on health literacy is necessary.