Biopsy collection and histopathological analysis
cutaneous lesions, ulcer, acanthosis, spongiosis
DYSKERATOSIS, ULCER, HYPERKERATOSIS, SPONGIOSIS
Biopsy samples from CL lesions were evaluated by expert pathologist before (day 0) and 8 weeks after intervention. The skin biopsy specimens were taken from the edge of cutaneous lesions with a 4 mm in thickness Harris punch, preceded by the application of local anesthesia and anti-septic agent. All specimens were fixed in 10% buffered formalin, and processed within a period of not more than 48 h to dispose of the paraffin tissue block. All specimens were dehydrated, cleared, embedded in paraffin, cut into 4 μm thick sections and stained with Hematoxylin–Eosin (HE). The histopathological changes in the epidermis (acanthosis, dyskeratosis, spongiosis, exocytosis, hyperkeratosis, and ulcer) before and after the last intervention was characterized using an optical microscope at 400 × magnification. At the same time, the intensity and distribution of inflammatory reactions in the dermis were evaluated.