2.8. Osteocalcin Concentration in Serum
The blood of mice was obtained by the orbital sinus method under anesthesia. The blood samples were centrifugated, and serum was collected. The concentration of serum OCN was estimated by an enzyme-linked immunosorbent assay (ELISA) kit (Immutopics, Inc., San Clemente, CA, USA). The procedures were conducted according to the manufacturer’s instructions.