2.7. Sample Collection and Storage
A quantity of 20 mL of blood was obtained upon admission and 48 h after treatment. Samples were identified as pre (0 h) or post sample (48 h). They were centrifuged at 3000 rpm for 20 min at 4 °C. Serum was stored in 3 or 4 Eppendorff aliquots of 1.5 mL and stored at <70° until processed.