2.4.3. Blood Samples
BLOOD
As mentioned above, an initial blood sample was obtained from the antecubital vein following a 12 h overnight fast at rest before and 72 h after the last training session. Additionally, a second blood sample was collected following (~5 min) the TTE. Blood samples were collected in Vacutainer© EDTA tubes for plasma and tubes with pro-coagulant (10 mL) for serum. Tubes were centrifuged for 10 min at 4 °C and 4000 rpm. Plasma and serum were aliquoted in Eppendorf tubes with 500 μL of sample and stored at −80 °C until analyzed.