2.4. Measurement in blood and stool samples
BLOOD
Blood samples were centrifuged at 1500 g for 10 minutes, and concentrations of JCP-specific IgE in collected serum samples were measured by fluorescence enzyme immune assay (SRL, Tokyo, Japan). Stool samples were collected in spoon-type collection tubes and frozen at −80°C. Concentration of organic anions and short-chain fatty acids in stool samples were measured by liquid chromatography (Techno Suruga Co., Ltd., Shizuoka, Japan). Fecal bacteria were analyzed by Techno Suruga Co., Ltd. using the terminal restriction fragment length polymorphism-based method, according to methods used in a previous study.