Anti–PD-1 treatment promoted the activation and proliferation of malignant T cells.
To dissect the response of malignant T cells and nonmalignant cells to anti–PD-1 treatment, PBMCs obtained from the patient before and after PD-1 blockade were subjected to single-cell RNA-Seq (scRNA-Seq). Six major cell types were identified based on the expression of canonical marker genes, inferred copy number variations (CNVs) (