RyR2 immunoprecipitation and oxidation assay from guinea pig left ventricle tissue
LYSED, LYSIS
Snap frozen guinea pig LV tissue was lysed using RIPA lysis buffer (Sigma, cat# R0278), supplemented with phosphatase (Roche, cat# 4906837001), and protease inhibitors (Roche, cat# 4693124001). RyR2 was immunoprecipitated from tissue lysate using anti-RyR2 antibody (Invitrogen Cat# MA3916, 5 μL) in 0.5 mL RIPA buffer overnight at 4 °C as previously described 