Bacterial community sequencing
DNA extraction was performed on 200 mg of stool sample using the DNeasy PowerSoil HTP 96 kit (QIAGEN) as per the manufacturers’ instructions. The purity of the genomic DNA was checked using the Nanodrop 1000 UV-Vis Spectrophotometer (ThermoScientific) with a A260:280 nm of >1.8 required to proceed to sequencing. Amplicon sequencing of the V4 region of the 16S rRNA gene was performed at NU-OMICs DNA sequencing research facility (Northumbria University) using the method described by (