Subgingival biofilm sampling
calculus
STERILE, PLAQUE
Subgingival biofilm collection was completed before any periodontal intervention following the removal of supragingival plaque and calculus using periodontal curettes. Samples for microbiological analysis were obtained by inserting a sterile number 30 paper endodontic point into the deepest periodontal pocket from each quadrant for 10 s. Samples were taken twice, at baseline before periodontal treatment and after 6 months. Samples were stored at -80 °C in sterile Eppendorf tubes until processing.