Discussion
hypoglycemic drug, human rhabdomyosarcoma, diabetic, T2DM, weight loss
PROLIFERATION, INSULIN SENSITIVITY
The present study aims to assess the effect of coadministration of glimepiride as a hypoglycemic drug and omega-3 fatty acids on the blood levels of irisin, sirtuin-1, glucose homeostasis, and lipid profile of type 2 diabetic patients.Our results revealed no significant difference in the case of body mass index between both studied groups after 3 months of intervention, which seems in accordance with some previous findings [In our study, we found that FPG, HOMA-IR, and HbA1C levels were reduced in the omega-3 group when compared to the control group, suggesting enhanced insulin sensitivity in the studied diabetic patients. This suggests that the consumption of omega-3 fatty acids supplements could improve glycemic control, FPG, HbA1c %, and HOMA-IR, in T2DM patients, which seems in accordance with some previous findings [The current study revealed no change in plasma insulin in response to omega 3. Our results agree with those of Mostad et al., [The present study indicated a significant reduction in total cholesterol (TC) after omega-3 fatty acids supplementation, which seems in accordance with some previous findings [Previous studies seem to be compatible with our current result, revealing that triglyceride levels and AIP decreased effectively among patients receiving n–3 fatty acids [Although both groups showed a significant reduction in LDL after 12 weeks, changes in LDL did not differ significantly between groups. Consistent with our results, Eftekhari et al. found that omega-3 fatty acids did not have any significant effect on serum LDL [The current study indicated that omega-3 supplementation resulted in an increased serum irisin level of type 2 diabetic patients after the intervention between both groups. The results of a study on human rhabdomyosarcoma cells have shown that treatment with omega-3 fatty acids for 24 and 48 h leads to increased expression of the irisin level [Another study stated that irisin promotes cell proliferation, as well as insulin production and secretion. Increased circulating irisin has been linked to better glucose tolerance and weight loss [Although serum sirtuin-1 level increased significantly in the omega-3 group, the changes in serum sirtuin-1 level did not differ significantly between groups in the present study. In line with our study, the addition of omega-3 fatty acids in a study on male Sprague–Dawley rats conducted by Son et al. resulted in a relative increase in SIRT1 expression, but this difference was not statistically significant compared to placebo group [