Translational Biomarker Analyses
nontumor, tumor
TUMOR
Changes in TILs and the ratio of Teff to Tregs (CD8In Part 1, a single-color, Singleplex chromogenic assay (Indivumed) was used to quantify cells in the combined tumor plus stromal (nontumor) regions in baseline and on-treatment biopsy samples. In Part 2, a five-color, Multiplex IHC assay (Indivumed) was used to quantify cell densities separately in the tumor and stromal regions, in addition to a composite of the total tissue section from baseline and on-treatment biopsy samples. The Multiplex assay measured CD3