2.3. Antimicrobial Susceptibility Profiling
SEPARATION, FRANKLIN, MRS
Antimicrobial susceptibility was determined by using the broth microdilution method and lymphocyte separation medium (LSM, including 90% IST medium [Cat. No. CM0473; Oxoid, Basingstoke, Hampshire, UK] and 10% MRS medium (Cat. No. 288130; Difco Laboratories Inc., Franklin Lakes, NJ, USA) in accordance with the guidelines of the Quality and Standards Authority of Ethiopia (ES ISO10932:2012). Twofold dilutions of clinically relevant antibiotics (clindamycin, chloramphenicol, erythromycin, gentamicin, kanamycin, streptomycin, tetracycline, and ampicillin, all from Sigma-Aldrich, Saint Louis, MO, USA) were prepared in LSM. Approximately 50 μL of 6 × 10