Viral shedding
VIRUS, VIRAL SHEDDING
Stool samples were obtained on Day 29 post-vaccination, before bOPV challenge, and then on Days 33, 36, 39, 43, 46, 50 and 57 (equivalent to Days 4, 7, 10, 14, 17, 21 and 28 post-bOPV challenge). Type-specific fecal viral shedding was assessed using reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA, and then total infectious virus, measured as 50% cell culture infective dose (CCID