Mosquito sampling
hemolysis, PSC
HEMOLYSIS
One data collection round occurred at baseline in September and October 2019 before the net distribution, while 8 post-net distribution rounds were conducted between June 2020 and April 2022. HLC and PSC collections were conducted in all 60 clusters every 3 months. CDC light traps were only conducted at baseline and during two round post-net distribution (16- and 19-months post-net distribution (June-July, and September–October 2021, referred to as Rounds 5 and 6)). For HLC sampling, one house was selected randomly in each cluster, with three further houses selected within 20 m of the randomly selected house (for a total of 4 houses per cluster per round). For PSC sampling, four houses close to the HLC selected houses were used. At baseline, one CDC light trap was placed in a house neighbouring a house used for HLC. The other three houses for CDC light traps were picked randomly in the cluster. Post-net distribution, the CDC light traps were used in houses neighbouring the four houses used for HLCs, similar to the PSC sampling method. There was a total of 240 collection points per round for each collection method.HLCs were conducted with one trained adult volunteers positioned inside each house. The first group of volunteers collected mosquitoes from 19:00 pm to 01:00 am and were replaced by a second group of collectors from 01:00 to 07:00. Collections were done using hemolysis tubes and a flashlight to collect mosquitoes that landed on their lower limbs (Fig. Mosquito collection methods used in the field, (Standard miniature CDC light traps (Model 512; John W. Hock Company, Gainesville, FL.) were set from 19:00 pm to 7:00 am at the foot of an occupied bed fitted with a mosquito net (a study net or other net) positioned around 70 cm above the floor (Fig. In houses selected for PSCs, white sheets were installed to cover the floor and furniture and an insecticide aerosol combining 0.25% transfluthrin and 0.20% permethrin (trade name Rambo) was sprayed (Fig. All collections were performed in households located in the core area of each cluster to prevent contamination from adjacent clusters that might have received different nets.