2.1. Materials and Reagents
EMD
Resveratrol, L-(+)-tartaric acid, quercetin, (+)-catechin hydrate sulfatase (Helix pomatia, Type H-1, sulfatase ≥ 10,000 units/g), and β-glucuronidase (Helix pomatia, Type HP-2, ≥100,000 units/mL) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 8-iso prostaglandin F2α and 8-iso prostaglandin F2α-d4 were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). (±)-Tartaric-2,3-d2 acid was purchased from C/D/N Isotopes (QC, Pointe-Claire, QC, Canada). Fisetin and formic acid (LC-MS grade) were obtained from TCI chemicals (Portland, OR, USA). LC-MS grade water and acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO, USA). A creatinine parameter assay kit was purchased from R&D systems (Minneapolis, MN, USA), and Millex-GV Filter (0.22 µm) was obtained from EMD Millipore (Burlington, MA, USA).