Measurement of total energy expenditure using doubly labelled water
BLIND
TEE was determined by using the DLW methodAll urine samples were stored at −18°C at the centre, before being aliquoted into 2·0 ml cryotube vials and frozen at −80°C. Once collection was complete, they were sent for analysis to the Energetics Research Group, University of Aberdeen.Analysis of the isotopic enrichment of urine was performed blind, using a Liquid Isotope Water Analyser (Los Gatos Research)