References
sweetener intervention-regulated VIP-genes
Schematic of the intervention study. After a run-in period without sweetener intake (7 d), study participants consumed a recombination of sweeteners (surrogate) or mineral water (control). Intervention period was 24 h.Transcriptional regulation by saccharin of the sweet taste receptor, cytokines, and their cognate receptors in isolated neutrophils in vitro. Isolated neutrophils from buffy coat samples were incubated with saccharin (100 µM) in comparison to RPMI medium for 24 h. (Intervention-derived plasma kinetics of sweeteners in healthy volunteers. Ten volunteers consumed a surrogate beverage containing soft drink-typical concentrations of acesulfame-K, cyclamate, and saccharin dissolved in mineral water (compare Relative transcript levels for sweet and bitter taste receptors in isolated neutrophils after stimulation with typical plasma concentrations of NNS in vitro. Isolated neutrophils from buffy coat samples were incubated with the different sweeteners at plasma-typical concentrations for 24 h, as indicated. Transcript levels were obtained from RT-qPCR and are given as 2Taste receptor transcript level kinetics during beverage-typical sweetener mix intervention. The RNA was isolated from primary neutrophils of study participants (The OPLS-DA and VIP score plots of transcriptional data between sweetener mix and water in healthy volunteers. (Gene ontology term network analysis of sweetener intervention-regulated VIP-genes. ((Sweetener-induced facilitation of fMLF-stimulated CaPlasma concentrations of sweeteners in healthy volunteers at different intervention times.Data are mean ± SD of 