Sample analysis
CLOT, BLOOD, SEPARATION
Blood samples were collected in serum separation tubes (SST) and ethylenediaminetetraacetic acid (EDTA) containing blood collection tubes. SST were spun at 2300 rpm for 15 min after being allowed to clot for 15 min. Serum creatine kinase and myoglobin were analyzed each day samples were collected using Labcorp services (Burlington, NC). Plasma aliquots were prepared from ethylenediaminetetraacetic acid (EDTA) containing blood collection tubes and stored in a − 80 °C freezer until analysis for the oxylipins after the study was completed.