Discussion
malaria
MALARIA
The comparison of three mosquito collection methods (HLCs, CDC light traps and PSCs) as part of a large RCT testing dual-a.i. LLINs in Benin showed that HLCs resulted in far greater numbers of mosquitoes being collected as compared to CDC light traps and PSCs. Overall, the mean density of The higher In the present study, PSCs resulted in a far lower (at least 10 (95% CI 9.8–11.7) times) mean indoor vector density compared to HLCs (Table Conflicting results regarding SR have been found in previous studies with some finding SR was similar for both HLCs and CDC light trapsBoth CDC light traps and HLCs were used to assess the effect of chlorfenapyr-pyrethroid and pyriproxyfen-pyrethroid LLINs on key entomological indicators (density, SR, and EIR) of malaria transmission as compared to pyrethroid-only net. Overall, the two collection methods showed that the chlorfenapyr-pyrethroid LLIN tended to be more effective than the pyriproxyfen-pyrethroid LLIN, but with weak evidence, as there were only two rounds of collection (Table PSCs and HLCs showed a significant reduction in vector density in the chlorfenapyr-pyrethroid LLIN arm. By comparison, PSCs displayed a significant reduction in vector density in the pyriproxyfen-pyrethroid LLINs arm, while HLCs did not (Table Overall, the same morphologically identified mosquito genera were collected using the three collection methods. The proportion of At the molecular species level, the composition was similar post-intervention for HLCs and CDC light traps as observed by Mawejje et al.Limitations for the present study include the only two post-net distribution rounds of data collection performed with CDC light traps, the lack of molecular (species identification, and To our knowledge, the present study is the first one to evaluate whether PSCs and CDC light traps are equally as effective as HLCs in estimating the differential impact of dual-a.i. LLINs on key entomological indicators of malaria transmission. Overall, HLCs collected more mosquito vectors than CDC light traps and PSCs, so remain the gold standard method to be used for entomological monitoring, notwithstanding the ethical issues involved and the fact that in the daily life, nobody in the community is going to sit and expose their lower limbs to receive potential mosquito bites overnight—so the mosquitoes caught might not be reflective of a realistic risk of biting. However, CDC light traps and PSCs were found to be as effective as HLCs in evaluating the differential impact of dual-a.i. LLINs on key entomological indicators of malaria transmission as compared to pyrethroid-only nets.