Lactic acid level measurement
painless
STERILE
Lactate Scout Plus device was used to evaluate lactic acid level. Lactate Plus (L+, Nova Biomedical) uses an electrochemical lactate oxidase biosensor to measure lactate in whole blood. A blood sample of 0.7 μL is required; Sample analysis time is 13 s. Test strips used with the device do not require calibration codes or special calibration strips. The device is equipped with a two‐level quality control solution that is used before testing to ensure correct operation of the analyzer (Tanner et al., Normally, a sample is taken from the earlobe to make a painless measurement. However, as VNS was performed through the ear, samples were taken from the middle finger of the participants’ right hand in order to prevent the effect of measurement from there on autonomic activity. In order to minimize the error rate of the data, the finger to be measured was cleaned with a clean and sterile cotton beforehand.