6. Limitations
BLIND
Although it was possible to control the study for different factors such as age, sex, and BMI, it is important to consider that it was not possible to blind the intervention groups, and this could have implications for biases. Especially for the control group, the only natural food coloring available in the market was the same Annatto, limiting the possibility of masking the control group to simulate whole egg. In addition, the use of a parallel randomized clinical trial is a limitation, since it does not consider the individual characteristics of the volunteers, which play an important role in their metabolic and physiological responses.