2.5. Intervention
All study participants (CG and IG) prior to randomization and after the baseline visit received brief nutritional counseling with recommendations on a balanced diet (see The IG participants received Málaga muscatel raisins (50 g/day) to add this supplement to their usual diet. In addition, they were given a calendar to record their consumption. No specific instructions were given regarding the time of intake, nor if its consumption had to be with or without other foods, they were decisions made by each participant according to their preference.The daily nutritional contribution of 50 g of Málaga muscatel raisins, as indicated on the label, is 150 kcal, 36 g of carbohydrates of which 14 g are sugar, 1 g of protein, 0.25 g of fat, and 0.02 g of salt.The analysis of the polyphenolic composition of the Moscatel de Málaga raisin grape was carried out with a mass spectrometer (HPLC-DAD-ESI/MS) using a high-performance liquid chromatography technique and quantified by comparing the chromatographic peaks against prepared calibration curves with the external standards of each compound. 