Preparation of P-MSCs
P-MSCs were harvested from placental tissue acquired from a full-term delivery with written informed consent from a healthy mother and delivered fresh tissue. After successful vaginal birth at a maternity hospital, the placenta was obtained from informed, healthy women (Additional file According to the current GMP (Good Manufacturing Practice) standards, all steps were carried out in a GMP-grade clean room facility. The fresh placenta was rinsed with PBS, divided into small pieces, and washed with 0.9% sodium chloride solution to remove any remaining blood before incubation for three hours at 37 °C with GMP-grade collagenase NB6 at 1 mg/mL (SERVA Electrophoresis GmbH). Following the addition of 0.9% sodium chloride solution, the mixture was shaken and centrifuged.The supernatant was withdrawn and the cell pellet was cultured on MSC complete medium with 10% pharmaceutical grade Australian origin fetal bovine serum (ATOCEL). Primary cultures were kept in a 37 °C humidified 5% COAccording to the International Society of Cell Therapy (ISCT), confirmatory tests were carried out, including flow cytometry (CD34, CD45, CD29, CD90, and CD105) (Additional file 