4. Discussion
metabolic diseases
METABOLIC DISEASES, MINERALIZATION
The interactions between diet and physical activity play a major role in the long-term regulation of body weight and physical fitness. Endocrine factors, including not only classical hormones but also adipokines, cytokines, and myokines, were shown to be very active players in this process [This study follows up on our earlier findings [Myokines, muscle-secreted molecules, play a crucial role in the regulation of muscle metabolism and function. Out of the myokine/adipokine/cytokine panel analyzed, the serum concentrations of three myokines were affected by the intervention. Musclin is a peptide produced by bone and skeletal muscle cells that plays an important role in the regulation of bone growth and physical endurance by enhancing the growth of muscle fibers [Osteonectin is an acidic extracellular matrix glycoprotein that plays an important role in bone mineralization and collagen binding [FGF21 is a novel metabolic regulator produced primarily by the liver, but to a certain degree also by other tissues such as white and brown adipose tissue, skeletal muscle, and the pancreas [In addition to the important role of myokines in the regulation of muscle regeneration and physical performance, these molecules may also represent a promising therapeutic target for metabolic diseases [For proper interpretation of the results of our study within the context of other published data, it is important to consider its strengths and limitations. The randomized design and the good compliance of the subjects to the dietary and treatment regimens are strong points of our trial. On the other hand, the limitations include a relatively short duration, a low number of subjects, and the inclusion of only male participants. Another limitation may be the use of bioelectrical impedance analysis instead of dual-energy X-ray absorptiometry, which is more accurate.