Serum lipid profile measure
TG, CDI
Serum lipid parameter levels were measured at the Centro Diagnostico Italiano (CDI) S.p.A (via Simone Saint Bon 20, 20147, Milano, Italy) by routine method on Atellica ® CH Analyzer (Siemens Healthcare Diagnostics Inc., 511 Benedict Avenue, Tarrytown, NY, USA) with the relevant commercial reagent kits. According to the manufacturer, the linearity through the measurement range is between 10 – 550 mg/dL (0.11 – 6.22 mmol/L) for TG; 20 – 129 mg/dL (0.52 – 3.34 mmol/L) for HDL; 5.0 – 1000.0 mg/dL (0.13 – 25.90 mmol/L) for LDL; and 25–618 mg/dL (0.65 – 16.01 mmol/L) for TC.The limit of blank (LoB), the limit of detection (LoD) and the limit of quantitation (LoQ) are 3 mg/dL (0.03 mmol/L), 10 mg/dL (0.11 mmol/L) and 4 mg/dL (0.05 mmol/L) for TG, respectively; 0.5 mg/dL (0.01 mmol/L), 2.7 mg/dL (0.07 mmol/L) and 2.1 mg/dL (0.05 mmol/L) for HDL, respectively; 1 mg/dL (0.03 mmol/L), 25 mg/dL (0.65 mmol/L), and 2 mg/dL (0.04 mmol/L) for TC, respectively; and an LoB of 0.8 mg/dL (0.02 mmol/L) and an LoQ of 3.0 mg/dL (0.08 mmol/L) for LDL.