Test group (PRF)
MARF
STERILE, PERIOSTEUM
Patient's blood was collected into 10 ml sterile silica‐coated plastic tubes without anticoagulants (VACUETTE; Greiner Bio‐OneOne GmbH, Kremsmünster, Austria). At least two tubes were prepared, and the tubes were centrifuged immediately in a fixed‐angle centrifuge (Arman Teb Noor, Iran, Tabriz). According to studies by R. Miron et al. (The MARF was done exactly as mentioned before. Several layers of PRF membranes (two or three layers) were placed on the exposed periosteum and were fixed by sutures around the teeth and finally dressed with a periodontal dressing (COE‐PAK™; GC Corporation, Alsip, Illinois, USA) (Figure All patients were prescribed 0.2% chlorhexidine mouthwash twice a day for 4 weeks, and ibuprofen 400 mg four times a day or as needed. The periodontal dressing and sutures were removed after 1 week. The patient was prohibited from doing any mechanical cleaning in the surgical area such as brushing and flossing for 4 weeks (Carnio et al., 