Preparation of oily solutions
Topical formulation of frankincense extract was prepared by Fardis pharmaceutical company, Isfahan, Iran. For preparing 100 ml of Boswellia solution, 1 g of dried extract of frankincense was added to 20 ml of black seed oil, then reached to the volume with olive oil. The placebo solution was prepared just with 20 ml of black seed oil and 80 ml of olive oil. Both types of solution were similarly packaged in pharmaceutical tubes and labeled and a numeric code was recorded on each tube by the company.