Exploratory outcomes
Bedside testing of total nucleated cells injected and viability for each cellular group was analyzed. In addition, 71 BMAC, 16 SVF and 8 UCT-MSC samples at the time of publication were subjected to single-cell RNA sequencing to reveal differences and similarities in the cellular components of each product via cell clustering analyses visualized in two dimensions using Uniform Manifold Approximation and Projection. Transcriptomic analyses at the single-cell resolution revealed that both autologous cell sources exhibited distinct cell subpopulations with some similarities in a subset of hematopoietic lineage-derived cells. Conversely, the UCT mesenchymal population, although exhibiting a defined clustering pattern, showed a uniform MSC phenotype.