Biological assays
external malformations
Peripheral blood was collected by tail vein puncture on the 16The fetuses underwent systematic analysis for external malformations and sexing and were then randomly distributed into two subgroups. The first subgroup was destined for visceral analysis. It was fixed in Bodians' solution, composed of distilled water (142 mL), acetic acid (50 mL), formaldehyde (50 mL), and 95% alcohol (758 mL), for at least seven days. They were then submitted to incision/microdissection with strategic cuts proposed by Barrow and Taylor [Photomicrograph of brain microdissections. Photomicrograph of rim microdissections. 