2.3. Primary and Secondary Measurements and Statistical Analysis
TG
On the first day of the study, anthropometric measurements of height and body weight, and the hemoglobin A1c (HbA1c) of the participants were measured at Kyoto Women’s University in the morning after an overnight fast. All blood samples were examined by Nihon Rinsho, Inc, Kyoto, Japan. Plasma glucose concentration was measured by HK-G6PDH methods (KANTO CHEMICAL CO., INC. Tokyo, Japan). HbA1c levels were determined by NGSP method (Kyowa Medix CO., INC, Tokyo, Japan). Serum insulin levels were determined by CLEIA method (FUJIREBIO. CO., INC. Tokyo, Japan). Serum TG concentrations were determined by GK-GPO method (SEKISUI MEDICAL CO., LTD. Tokyo, Japan). Serum FFA concentrations were determined by ACS-ACOD method (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan).In the present study, a total of fourteen participants was calculated as the sample size to provide 95% power to detect 5% difference in postprandial blood glucose levels (G*Power 3.1, Heinrich-Heine-Universität Düsseldorf, Germany), matched to our study of consuming test meals of different food order in young, healthy women [