Introduction
Edited by: Mahbuba Rahman, McMaster University, CanadaReviewed by: Samiksha Garse, DY Patil Deemed to be University, India; Mathieu Garand, Washington University in St. Louis, United States; Tengchuan Jin, University of Science and Technology of China, China†These authors have contributed equally to this workThis article was submitted to Systems Immunology, a section of the journal Frontiers in ImmunologyOver the last decade, the field of systems vaccinology has emerged, in which high throughput transcriptomics and other omics assays are used to probe changes of the innate and adaptive immune system in response to vaccination. The goal of this study was to benchmark key technical and analytical parameters of RNA sequencing (RNA-seq) in the context of a multi-site, double-blind randomized vaccine clinical trial.