Hormonal assays
All patients provided serum specimens prior to anesthesia, as well as at one week and six months following the procedure. Venous blood samples were obtained, and serum was extracted by centrifugation. According to manufacturer’s instructions, serum E2 and P levels were measured by enzyme-linked fluorescent assay (ELISA; Beckman Coulter Inc., Ireland). Serum AMH level was measured by a commercially available enzyme-linked immunosorbent assay kit (ELISA; Beckman Coulter Inc., Ireland) and reported as nanograms per milliliter with a detection limit of 0.16 ng/mL. Postoperative serum AMH level and AMH decline were the primary outcome measures.