4.8.5. Carbonylation Protein Concentration
Briefly, 100 µL of plasma were added to 500 µL of HCl 2.5 N in parallel with another sample with 500 µL of 2, 4-dinitrophenylhydrazine (DNPH) and incubated. At the end of the incubation period, they were centrifuged at 15,000× 