Validation of proteomics data for selected proteins by ELISA
CKD
Among the top 10 proteins that were significantly expressed and significantly enriched after exercise, MMP-9, ACVR1B, and FETUB were selected for the validation of proteomic data obtained using ELISA. The logThe baseline levels of MMP-9 and FETUB were higher in patients with CKD on dialysis than those in the healthy age-matched control group, and the expression level significantly decreased after exercise. In contrast, the level of ACVR1B was lower in patients on dialysis than that in healthy controls, and the level significantly increased from the baseline after three months of exercise (Fig. 
Validation of proteomics results with ELISA. Age-matched healthy control, before exercise, and after 3 months exercise. *P < control vs. before exercise; **P < control vs. before exercise; ***P < before exercise vs. after exercise. Control, Age-matched healthy control; before, before exercise in dialysis patients; after, after 3 months exercise in dialysis patients