Instrumental conditions
SEPARATION
Both lipidomic and metabolomic analysis used an Infinity 1290 (Agilent) UHPLC coupled to the 6560 Ion Mobility Q-TOF LC/MS (Agilent) with an Agilent Jet Stream (AJS) electrospray (ESI) source. The details of the lipidomic method are described elsewhere (10.3390/metabo12020124), briefly an Acquity BEH C18 column (1.7 μm, 2.1 mm × 150 mm; Waters, USA) was used for chromatographic separation. Lipids were separated by a gradient of mobile phases A: 10 mM ammonium formate and 0.1% formic acid in acetonitrile:water (60:40, v/v); mobile phase B was 10 mM ammonium formate and 0.1% formic acid in 2-propanol:acetonitrile (90:10, v/v) in ESI + mode, for ESI- mode formic acid and ammonium formate were replaced by acetic acid and ammonium acetate.The details of metabolomic method are described in the supplement. Briefly, metabolites were separated on an Acquity HSS T3 column (1.7 μm, 2.1 mm × 100 mm; Waters, USA) using a gradient of mobile phases A: 5 mM ammonium formate and 0.1% formic acid in water:MeOH (95:5, v/v) and mobile phase B: 5 mM ammonium formate and 0.1% formic acid in MeOH in ESI + mode, for ESI- mode formic acid and ammonium formate were replaced by acetic acid and ammonium acetate. The mass spectrometer acquired data in Full MS and autoMS/MS modes in the 