2.13. Quantitative Real-Time Polymerase Chain Reaction (qPCR) Analysis of Gut Microbiota DNA Concentration
In this study, the researchers aimed to quantify the concentration of gut microbiota DNA through quantitative real-time polymerase chain reaction (qPCR) analysis. The DNA concentration was determined by measuring the absorbance at 260 nm (A260), while its purity was assessed by calculating the A260/A280 ratio using a Multiskan™ 1500 spectrophotometer (Thermo Fisher Scientifics; Waltham, MA, USA). For PCR analysis, group-specific primers (