DNA and RNA extraction
tumor, Tumor
BLOOD, TUMOR, TUMOR
After the evaluation of the tumor cell content in FFPE tumor specimens by a pathologist, samples were macro-dissected to obtain at least 80% tumor cell content for nucleic acid extraction. DNA was isolated from tumor tissue using the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega). DNA from whole blood (germline DNA) was isolated using the Maxwell 16 Blood DNA Purification Kit (Promega) following the manufacturer’s instructions. The quantity of extracted genomic DNA was assessed by a fluorometric method with a Qubit device. RNA was extracted using the Maxwell RSC RNA FFPE Kit (Promega) according to the manufacturer’s protocol. DNA and RNA quality and quantity were assessed by spectrophotometry with absorbance at 230 nm, 260 nm and 280 nm. Tumor purity was reported in a table for each exome and RNA-seq data where information was available (Supplementary File Table 