infection
LYSED, INFECTION
Monolayers of Vero-E6 cells were grown to confluence on 96-well plates and infected with the patients’ samples previously diluted 1:2 with infection medium (Minimum Essential Medium -MEM with 0.2% BSA 0.2%, 2mM glutamine and 20 mM Hepes) and incubated for 4 h at 37°C. After removing the inoculum, Eagle’s MEM with 10% foetal bovine serum and antibiotics was added to each well of infected cells. Non-infected cells were used as negative controls. After 72 h, cells were collected, lysed using Dynabeads™ MyOne™ Silane beads and the number of present SARS-CoV-2 genomes analysed by RT-PCR (Detecting SARS-CoV-2, BGI, genes ORF1ab and Human β–actin). These values are expressed as 2^(-ΔCt)*1000.