2.1.2. Preparation of Slides
The slides were cleaned and labeled properly, curved in freshly prepared hot 1% NMPA, and placed in refrigerator for at least 2–12 hours. After that, 10 Class 0 = undamaged cells.Class I = cells with head diameter of nucleus equal to or greater than tail length.Class II = ccells with head diameter of nucleus less than tail length although tail length less than the double of head diameter.Class III = cells with tail length greater than the double of head diameter.