Histological evaluation
DECALCIFICATION, DECALCIFICATION
After the completion of the endodontic procedures, all samples were fixed in 10% (v/v) neutral buffered formalin for 48 h and then washed under running tap water for 1 h. Decalcification of teeth was performed using a 5% nitric acid solution, with fresh solution being replaced every two days. The endpoint of decalcification was determined using a chemical method previously described in a study [Representative histological picture from root canals section; (