Immunohistochemistry (IHC) of FAP expression
FAP
FAP expression in 4 representative patients was analysed by immunohistochemistry, heat-mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0. The sections were incubated with 1:250 humanized anti-fibroblast activation antibody (Abcam, ab207178) at 4 °C overnight. After incubation with the labelled streptavidin–biotin (LSAB) complex, the slides were stained and visualized using the iView DAB detection system (ZSGB-BIO, Beijing, China). Typical lesions in high-power fields were photographed for visual comparison.