Conclusions
alertness
We report herein on the temporal profile of IN-OT in the human ANS at rest using HRV and, for the first time, pupillary unrest measures. Having found OT to decrease PUI (suggesting PNS deactivation) and tentatively (as an exploratory finding) that it may increase HF-HRV (suggesting PNS activation), we speculated that both might be consistent with the facilitation of alertness and preparedness for an approach behavior. Given the early days of IN-OT and ANS association research, the interpretation of these results remains speculative. Nevertheless, we hope our findings may assist in future study design, in the investigation of the comparability between IN-OT findings across data modalities, and in the assessment of usefulness of ANS markers for IN-OT response monitoring and human social cognition understanding.