2.5. Measurement of Short-Chain Fatty Acids (SCFAs)
Rtx-1701
The measurement of SCFAs, including acetate, propionate, butyrate, isobutyrate, valerate, and isovalerate, was performed via Gas Chromatography—Mass spectrometry (GC/MS) (Shimazu, Kyoto, Japan) on Rtx-1701 columns (Restec, Bellefonte, PA, USA). GC/MS samples were prepared as follows: 50 mg (wet weight) of fecal sample was suspended in 300 µL of pure water. The suspension was centrifuged at 15,000× 