Cell viability assays
Tecan
PROLIFERATION
A total of 2000 cells were seeded in 96-well plates in triplicate and treated with the indicated drugs at various concentrations or not treated for 96 h. Cell viability was measured by the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions, and luminescence was read using an Infinite M200 plate reader (Tecan, Switzerland). Half maximal inhibitory concentration (IC50) values were calculated, and proliferation curves were plotted using GraphPad Prism.