Isolation of PB mature lineages, BM progenitors and HSPC subpopulations
SEPARATION
PB and BM mononuclear cells from WAS-GT patients were isolated using Ficoll-Hypaque gradient separation (Lymphoprep, Fresenius). Mature PB lineages and BM progenitors were purified using positive selection with immunomagnetic beads (average purity, 94.6%) according to the manufacturer’s specifications (Miltenyi Biotec). HSPC subpopulations were FACS purified with the MoFlo XDP cell sorter (Beckman Coulter) as previously described