Reduction potential of KDP and WHEY
The accessible thiol content was determined in KDP and WHEY to assess the reduction potential. The proteins were extracted, and the diluted protein solutions were assayed by reaction with monobromobimane (MBB) and comparison to a GSH standard curve. 1 mM GSH (in 1 mM TCEP, 2.7 mM KCl, 0.14 M NaCl, 8 mM Na