Anti-adenovirus antibodies
ADENOVIRUS
Anti-hexon IgG antibody levels were determined before and after treatment by ELISA from patient serum samples according to the manufacturer’s instructions (Adenovirus IgG ELISA Kit; DEIA309; Creative Diagnostics). Absorbance at 450 nm was measured using a Synergy H4 plate reader (BioTek), and concentrations calculated on the basis of a standard curve (Gen 5 software Version 3.0, BioTek). Anti-adenovirus IgG serum concentration increases of fourfold or greater were considered seroconversions. A more stringent threshold of tenfold or greater increases in levels of anti-adenovirus IgG serum concentrations was also tested.