Metabolomics analysis
ten-year-old obese
Serum samples were derived from our prior randomized control trial (NCT03628937). Briefly, researchers assigned six to ten-year-old obese girls to two groups: placebo and EGCG. Twelve weeks of EGCG capsules (200mg, 50% EGCG) were given to girls in the EGCG group (n=18). The placebo group (n=16) received placebo capsules that appeared the same for twelve weeks. Detailed descriptions of the research design have been provided previously (Serum metabolomics were analyzed using a high-performance liquid chromatography-electrospray ionization ion-trap tandem mass spectrometry (LC-ESI-MS/MS) in both positive and negative ion modes. Chromatographic conditions were based on the prior description (Data analysis was carried out utilizing Majorbio Cloud (