2.2. Participants
In total, 25 healthy men and women completed all study visits (see The participants were instructed to follow their normal diet and activity patterns throughout their participation in the study. The participants were required to complete a 24 h diet record prior to arriving at the laboratory for their initial screening visit. The participants were given a copy of this dietary record and instructed to duplicate all food and fluid intake 24 h prior to each subsequent laboratory visit. Prior to each subsequent visit, the participants were asked to verbally confirm their 24 h prior diet adherence and ensure they had a normal night’s rest. In addition to replicating food and fluid intake for 24 h prior, the study participants were also asked to refrain from exercise and alcohol 24 h prior, abstain from caffeine 12 h prior, and arrive 8 h fasted to all testing sessions. Again, these instructions were all verbally confirmed at the beginning of each study visit.