2.3.3.4. Comparing the level of changes in immune function indexes in the 2 groups.
fasting venous blood
First, 5 mL of fasting venous blood was collected from patients in both groups before and after treatment. After centrifugation at 3000 r/min for 10 minutes, the supernatant was taken for pre-treatment. The changes in the expression levels of helper T cells 17 (Th17) and regulatory T cells (Treg) were detected using a flow cytometer (Serena [China] Medical Technology Co., Ltd, model Sparrow), and the operation procedure was performed strictly according to the manufacturer instructions.