Donor selection and blood sampling
infection
INFECTION, COLD
Allogeneic unrelated healthy donors were randomly chosen from healthy volunteers after signing the consent form. All volunteers have been proven to have a negative active infection of SARS-COV2 via the gold standard RT-PCR test. The HIV, HBV, HCV, and blood culture examinations were also performed for all donors, and all results were negative. Finally, 70 ml of whole blood was obtained from the donors by intravenous blood sampling using blood collection syringes and injectors in heparinized falcon tubes. The samples were immediately transferred to a cleanroom under cold conditions. Donor selection is based on whether donors have either maximum KIR incompatibility against recipients or a potent KIR B haplotype.