ENAMEL TI LAYER
tooth
One blinded operator covered 20 specimens with a carbon layer to be observed by scanning electron microscopy (model no. JSM 6460LV; JEOL Ltd., Tokyo, Japan). For each tooth, electromicrography was performed, and Ti mapping through the microprobe analysis with an energy dispersive spectrometer was completed (Noran System SIX, model no. 200; Thermo Fisher Scientific, Waltham, MA, USA) to determine the Ti coat presence.