Immunohistochemical results
CD68 immunoexpressing cells appeared as a brown cytoplasmic reaction. Immunostaining with CD68 revealed the presence of macrophages. Few CD68-positive cells and macrophages at the subepithelial level were detected in the Control group. In addition, some CD68-positive cells were demonstrated in the Vitamin D group. In contrast, numerous CD68-positive cells, and macrophages were detected in the epithelial covering and at the subepithelial level in the Placebo group. (Fig. Photomicrographs of palatine tonsil sections stained with immunohistochemical stain for CD68 [Avidine biotin peroxidase stain with Hx counter stain X 400, scale bar = 50 µm] showing: (