Sample size
There is no relevant comparative experimental research before, so we did not use data from the literature to calculate the sample size. Based on the previous pilot experiment, the main outcome measurement index VAS was used to estimate the sample size, assuming that the effect size was 0.4, using the G*Power3.1 sample size calculation software, and following the calculation method of the F test repeated measurement analysis of variance between groups, set the group as 4 groups, the number of repeated measurements as 5 times, set the effect size as 0.25, the significance level 