Quantification and statistical analysis
Work done in the BSL3 facility at the Icahn School of Medicine at Mount Sinai was done on NHBE from a single donor, repeated in three experimental repeats with three or more technical repeats in each experiment. Work done in the BSL3 facility at the Sheba Medical Center or in the BSL2 facility at The Hebrew University of Jerusalem was done on NHBE from two donors, repeated in three experimental repeats each (unless noted otherwise by the n value) with three or more technical repeats in each experiment. Work done in the BSL3 facility at the Sheba Medical Center in different variants was done separately and independently for each variant and repeated as listed above.Measurements were technically repeated three or four times for each sample, images were analyzed with five or more fields of view; Graphs show mean ± SEM; Continuous variables were compared with a Mann-Whitney U test or a two-sample t-test or ANOVA. Categorical variables were compared with a chi-squared or Fisher’s exact test, as appropriate. FDR correction was used to adjust for multiple comparisons and RNA seq comparisons; Hypergeometric testing was used to assess statistically significant enrichments. * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001, unless denoted otherwise.