Metagenomic sequencing
HT
Fecal samples were extracted by Diversigen with PowerSoil Pro (Qiagen) automated for high throughput on the QiaCube HT (Qiagen), using Powerbead Pro Plates (Qiagen) with 0.5 and 0.1 mm ceramic beads. Samples were quantified with Quant-iT PicoGreen dsDNA Assay (Invitrogen). Libraries were prepared with a procedure adapted from the Illumina DNA Prep kit (Illumina) and sequenced on an Illumina NovaSeq using paired-end 2×150 reads (Illumina) targeting a mean read depth of at least 4 million reads per sample.