Conclusions
PLAQUE
The total number of bacteria in the saliva increased significantly after brushing compared to before brushing. Mouthwashing was an effective method of collecting contaminated saliva after brushing. Poor oral hygiene and a decrease in the number of remaining teeth were identified as factors associated with an increase in the total number of bacteria in saliva before brushing; however, the amount of plaque did not directly affect the number of bacteria in saliva.