2.2. Preparation of NRE and Quantification of Its Active Compounds 
To prepare the NRE infusion, the dried leaf powder was steeped in 100 mL of boiling water for 15 min. The NRE dose was standardized using a validated high-performance liquid chromatography (HPLC) method (UHPLC-DAD, SHIMADZU 8045, Kyoto, Japan), encompassing qualitative and quantitative analysis. Two doses were identified and administered: a low dose containing 5 mg of flavonoids with 4 µg of isorhamnetin and a high dose containing 20 mg of flavonoids with 16 µg of isorhamnetin.