4.5. Blood Collection and Biochemical Analyses
TG
The first 2 mL of each blood sample drawn were discarded, followed by collection in three different tubes (VacuetteGlucose in plasma and total triglycerides (TG), low-density lipoprotein cholesterol (LDLc), high-density lipoprotein cholesterol (HDLc), and total cholesterol (TC) in serum were measured using an enzymatic and colorimetric assay on a Pentra C400 Clinical Chemistry analyzer (HORIBA Medical, Montpellier, France). Insulin was determined by ELISA (Mercodia AB, Uppsala, Sweden). 