Serum I-FABP and LPS
GI distress, ®, CusaBio
I-FABP is a surrogate marker of enterocyte damage and was analysed in serum with a Quantikine human ELISA kit (R&D Systems, Inc. Minneapolis, MS, USA), with inter-assay CV ≤ 10% across the assay working range of 3.6–1000 pg/mL. Systemic LPS concentrations are used as a surrogate marker of GI distress, with increases suggesting greater disruptions in GI integrity. Serum LPS was measured by CusaBio ELISA kit (CusaBio Technology LLC, Houston, TX, USA), with inter-assay CV of ≤ 12% across the measurement range of 6.3–400 pg/mL. Anti-LPS antibodies were measured with a commercially available ELISA kit (HK504, EndoCAb® IgM, Hycult Biotech, Uden, Netherlands); CV for this analysis was ≤ 7.2%.