Conventional IVF
IVF was performed using the technique of a single well with continuous culture
medium supplemented with 10 % synthetic serum (Ingamed®) and a
concentration of 40.000 sperm recovered/ml, with a volume of 800 µL and
covered with 200 µL of mineral oil. The inseminated cumulus oophorus
complex were cultured for five or three days according to the group inserted,
without handling of the plate and the incubator, under 7 % CO