Flow cytometry and ex-vivo stimulation assays
FRANKLIN
In this report, we focus on samples obtained pre-treatment and after cycles 1–3 to discover potential early biomarkers of clinical outcomes. Analysis focusing on additional cycles and the effects of SABR on the immune response will be reported elsewhere. PBMCs were immunophenotyped using flow cytometry. Briefly, PBMCs were thawed and incubated with Fc-block (BD Bioscience, Franklin Lakes, NJ) on ice for 15 min. Then, cells were stained with specific antibody cocktails (supplemental Table 