2.5. Metabolic Health
Plasma glucose (G75171L) and lipids (total cholesterol, C75101L; HDL-C, H751160; triglyceride, T7532500) were measured on a Synergy H1 microplate reader (Bio-Tek, Washington, DC, USA) using separate clinical assays (Pointe Scientific, Canton, MI, USA). Intra- and inter-assay coefficient of variations (CV) were 1.1–5.5% and 2.7–8.0%, respectively. LDL-cholesterol was calculated according to the Friedewald equation [