Intervention and test products
tooth brushing from mouth
We provided all the subjects with a standardized enzyme-free toothpaste with 1450 ppm sodium fluoride to be used twice daily during the course of the study. After the baseline clinical registration, we asked the participants to rinse their mouth thoroughly by swishing 10 ml of the assigned liquid around the teeth morning and evening for 30 seconds. After rinsing, the subjects should spit out, avoid water rinsing, and most importantly, we requested them to separate tooth brushing from mouth washing by at least 30 min. The duration of the intervention was 8 days. The participants were instructed to return the dispensed mouthwash tubes to the clinic at the next visit. The experimental mouthwash contained an enzyme blend of DNase, mutanase, and beta-glucanase (BioFreshContent of the experimental mouthwash.