Determination of heart infarct size.
Necrotic
NECROTIC
At the end of reperfusion, hearts were frozen at –20°C and sectioned into 1 mm–thick slices from the apex to the base, stained with triphenyltetrazolium chloride for 15 minutes, and fixed in 1% formaldehyde for 18 hours. Necrotic negatively stained myocardium was measured using Adobe Photoshop Elements 2019 Edition by an investigator blinded to group allocation (