Ultra high-performance liquid chromatography untargeted metabolomics
BLOOD
Blood samples (in lithium heparin tubes) were centrifuged at 2500 g for 5 min and the protein-free supernatant removed and stored at −80 °C before transfer for metabolomic analysis. Metabolites were extracted from plasma using a dual phase Bligh-Dyer extraction and analysed using a combination of hydrophilic interaction liquid chromatography (HILIC) and Reverse Phase Ultra High-Performance Liquid Chromatography-mass spectrometry (UHPLC-MS) for polar and non-polar/lipid metabolites respectively