Preparation of SVF
LYSED
First, 100 cc of fat was removed from the thigh area of each patient. The tissue was then washed with phosphate-buffered saline (PBS) (Miltenyi Biotec, Cologne, Germany) to remove red blood cells and leukocytes. The adipose tissue was digested with collagenase type I (Worthington Biochemical Corp, Lakewood, USA) for 20 min at 37 °C to produce a collagenase solution with a concentration of 0.1%. Enzyme digestion was prevented by washing with DMEM 10% FBS (Invitrogen, Carlsbad, USA), and floating and lysed fat cells were discarded. SVF cells were pelleted by centrifugation at 500The viability of isolated SVF cells was evaluated in the laboratory using an automatic cell counter. Flow cytometry was performed to analyze the surface marker expression of SVF cells. The data analyses were conducted using Partec—CyFlow ML. Data analysis was carried out using FloMax® software.