Statistical analysis
Data so collected was tabulated in an excel sheet, under the guidance of statistician. The means and standard deviations of the measurements per group were used for statistical analysis (SPSS 22.00 for windows; SPSS inc, Chicago, USA). For each assessment point, data were statistically analyzed using one way ANOVA. Difference between two groups was determined using student t-test as well as chi square test and the level of significance was set at 