Treatment of a human adipocyte cell line with ANP reduced leptin secretion
Media
Leptin is an adipocyte-derived hormone. Therefore, we aimed to understand by which mechanism ANP reduces leptin levels. Using human SGBS adipocytes, an ANP inhibits leptin secretion in human adipocytes. SGBS adipocytes were treated with 10 nM ANP or vehicle in serum-free basal medium. Media supernatants, RNA and protein were harvested after 24, 48, and 72h. 