Western blotting
LYSIS
Phosphorylation/total protein content of mTOR and downstream proteins (p70S6K, S6K, and 4EBP1) were analyzed at pre- (day 14) and post-immobilization (day 28) to investigate the anabolic effect of FOR supplement during immobilization. After snap frozen muscle tissues were homogenized with ice-cold lysis buffer (10 μL·mg