Inflammatory Response
SIRS
PCT
The occurrence of severe SIRS was defined by the presence of three or more of the
following criteria during the first 48 hours after surgery: temperature <
36.0 or > 38°C, heart rate > 90 beats/minute, respiratory rate > 20
breaths/minute or PaCO₂ > 32 mmHg, and leukocyte count > 12 or < 4
(10The EMP release profile was determined by flow cytometry, preoperatively and 24
hours and three months after prosthesis implantation. For EMP recovery, the
patients’ blood samples were collected using tube containing citrate
anticoagulant. Immediately after collection, they were centrifuged at 3000
× g for six minutes at room temperature, followed by another two
consecutive centrifugations at 3000 × g for 15 minutes at 4°C, after
incubation with the incubation antibody mixture (with anti-CD144, anti-CD31, and
PE-conjugated anti-CD42 monoclonal antibodies), a wash was performed by
centrifuging the sample at 20000 × g for 10 minutes before reading in the
cytometer. EMP were defined as CD31+, CD42-, and CD144+ labeled vesicles. The
plasma concentration of IL-6 and IL-8 was determined by multiplex technology
using the Luminex® 100TM detection system (Luminex Corporation, Austin,
Texas, United States of America). Procalcitonin (PCT) concentrations were
determined using the Abcam® Human Procalcitonin ELISA Kit (ab100630). The
ultrasensitive C-reactive protein (CRP) was analyzed by the automated
turbidimetric immunoassay method, and the leukocytes were analyzed by an
automated laser optical system, the Abbott Cell-Dyn Ruby device.