Sample preparation for glycan structure analysis
dryness
Frozen samples of MDCF-2, RUSF, their respective ingredients and faecal biospecimens were ground with a mortar and pestle while submerged in liquid nitrogen. A 50 mg aliquot of each homogenized sample was lyophilized to dryness. Lyophilized samples were shipped to the Department of Chemistry at the University of California, Davis. On receipt, the samples were pulverized to a fine powder using 2 mm stainless-steel beads (for foods) or 2 mm glass beads (for faeces). A 10 mg ml