Sequencing statistics
A summary of the reference alignment statistics is listed in Summary of human reference alignment statistics.Taken together, these summary statistics demonstrated that, in general, the replicate samples were sequenced effectively at each site. However, despite using replicate PBMC samples as starting material and identical sequencing platforms, sequencing depth metrics and mapping statistics varied. Variation was less pronounced for the 3 external UHRR control samples whose sequences were combined prior to processing (