Human leukocyte antigen (HLA) typing
Based on the DNA sequencing data, patients’ HLA class I genotypes (HLA-A, HLA-B, and HLA-C) were assessed using the OptiType software. Based on the RNA clean reads, HLA class II genes (DQA1, DQB1, DRB1, DRA, DPA1, and DPB1) encoding MHC class II receptors were analyzed based on Seq2HLA (V 2.3).