2.4. Ethics and Statistics
The study protocol complied with the principles of the Declaration of Helsinki (2013). Written informed consent was obtained from all participants. The current protocol was approved by the Institutional Ethics Committee of Cadiz (Cadiz, Spain). The results are expressed as mean (SD). The Shapiro–Wilk test was used to assess whether the data were normally distributed. To compare the mean values, one-way analysis of variance (ANOVA) with post hoc Bonferroni correction was used to account for multiple tests. For all tests, statistical significance was set at an alpha level of 0.05. 