Oral corticosteroids suppress IMQ-driven cytokine responses in blister exudate and whole blood cultures
In addition to the dermal cellular response, cytokine levels were analyzed in the blister exudate to evaluate of NF- κB- and IRF7-driven responses. IMQ application induced a mild IL-6 response at 24 and 48 h (Prednisolone suppresses the NF-κB driven cytokines and IRF7 driven response in blister fluid. Whole blood samples, drawn from study participants at predefined time points, were stimulated with IMQ for the evaluation of Ex-vivo whole blood stimulated with 20 μg/ml IMQ. 