Lipid mediator profile in PWH and SN individuals
PWH
HEAT
To understand the profile of pro-inflammatory and pro-resolving lipid mediators in PWH (i.e. baseline: pre-aspirin intervention) and SN individuals, we measured plasma levels of 50 lipid mediators belonging to 12 lipid mediator families derived from four major fatty acids namely docosahexaenoic acid (DHA), n−3 docosapentaenoic acid (n−3 DPA), eicosapentanoic acid (EPA) and arachidonic acid (AA) (Abundance heatmaps of the overall population (PWH and SN combined) at the lipid mediator family level, showed that while there were differences among individuals, there was no clear clustering by HIV status at either the metabolome or individual mediator level (Lipid metabolome and mediator abundance bar plot. A: Metabolome Abundance bar plot. B: Lipid mediator abundance bar plot. Abundance heat map for metabolome (A, left panel) and lipid mediator (B, right panel) for all individuals (N = 217) are shown as a stacked bar graph. Metabolome and individual mediators were logDifferences in metabolomes by HIV status.Analysis of covariance was conducted to assess the differences in metabolomes by HIV status. All models are adjusted for sex, race/ethnicity, age, body mass index, smoking, drinking and statin use. FDR: false discovery rate.PWH Geometric LS Mean/SN Geometric LS Mean.Based on ANCOVA analysis, adjusted for covariates, of 16 metabolites with VIP > 1, PWH had lower levels of 4S,14S-diHDHA (Δ = 0.77 (0.64, 0.93); unadjusted p