Differential changes of gene expression analysis linear over time
A workflow diagram of the study is shown in Figure Overview of analysis workflow and quantification of differentially expressed protein‐coding RNAs produced by linear mixed‐effects modeling (LMM) with linear time dependency. (a) A flow‐chart diagram depicting sample preparation and analysis workflow. (b) A Volcano plots depicting results of differential changes of gene expression between CR and AL using LMM and linear time. Red dot (positive beta: For in‐depth characterization, we focused the differential gene expression (DGE) analysis on the top set of the 199 genes (out of 1003 as mentioned above) whose expression changed differentially over time at the threshold of Genes showing differential expression changes in CR compared to AL participants during the 24‐month CR intervention.
