2.2. Blood Sample Collection and Processing
tumor
BLOOD, TUMOR, FRANKLIN
Whole blood samples (20 mL) were collected in K2-EDTA tubes (BD, Franklin Lakes, NJ, USA) before the first cycle (C0) and at the fourth cycle (C4). The C4 samples were collected between the tumor response assessment date (after the third cycle) and the start of the fourth cycle. If the treatment was terminated before C4, blood samples were collected at the end of treatment (EOT). The samples were transported to the central laboratory (Asan Medical Center, Seoul, Republic of Korea), plasma cell-free DNA (cfDNA) was acquired, and the bTMB was analyzed.Blood samples were centrifuged at 1000× 