Human SEB functional assay
The functional activity of tebotelimab to enhance IFN-γ secretion was evaluated in an antigen-driven assay model. In this model, staphylococcal enterotoxin B (SEB) was used as a superantigen in a re-stimulation assay, whereby PBMCs were first stimulated (primed) to enhance PD-1 and LAG-3 expression and then re-stimulated (boosted) again in the presence of tebotelimab to further enhance IFN-γ secretion. Human PBMCs were stimulated with SEB for 2 d, washed twice and re-stimulated with 0.5 ng ml