Discussion
PBC
COLD, PBC
To our knowledge, this study is the first to investigate the mobilisation of lymphocytes after a single PBC or CWI treatment compared to a control. As hypothesised, a single cold exposure increased the circulating numbers of specific PBMC subsets, namely CD16It is well evidenced that preferential mobilisation of tissue-homing and cytotoxic cells often occurs because of an increase in epinephrine, suggesting that there is a functionally relevant response in the specific demargination of cells in response to stress (Bosch et al. Hypothermic stress as a result of exposure to cold environments and cold water has been shown to increase circulating catecholamine concentration and subsequently influence lymphocyte mobilisation (Jansky et al. No alterations were observed in the number of circulating conventional monocytes with any treatment group. Between rest and post-treatment, an increase in B-cell counts was recorded in the CON group, but not in either PBC or CWI groups. This result, while statistically significant, is likely a result of intrasubject variation between time points. Differences in non-classical monocyte numbers between PBC and CON were found only at rest and are therefore likely due to inter-subject variation between these treatment groups at baseline.While the increase in CD16