Microbiome analyses: DNA isolation and amplicon sequencing
Microbiota from the tongue and faeces were characterized by sequence analysis of the V4 hypervariable region of the 16S ribosomal gene. The V4 hypervariable region provides profiles that adequately represent diverse communicates at the genus level, we aimed to obtain an overview of the microbial composition of the tongue, without necessarily achieving differentiation at the species level [