Author contributions
J.C. conceived the experiments, J.C., J.J. conducted the experiments, M.G.A.—prepared the patients and performed the TCA procedure, J.C. developed the image processing methodology, M.B. performed the statistical analysis, J.C. and M.B. analyzed the results, J.C.,J.J. and M.B. prepared the manuscript, L.P. and A.P. prepared the trial, E.P. managed the project. All the authors reviewed the manuscript.