2.5. Caco-2 Cells In Vitro Experiments
Differentiated Caco-2 cells (ACC 169, DSMZ, Braunschweig, Germany) were grown in trans-wells by using DMEM medium containing 10% fetal bovine serum (Pan-Biotech GmbH, Aidenbach, Germany) and 100 µg/mL streptomycin and 100 U/mL penicillin (Pan-Biotech GmbH, Aidenbach, Germany) in a 5% carbon dioxide atmosphere. After reaching confluency, cells were treated with sucralose (0.016 mM) or sucrose (10 mM) in the apical compartment of the trans-well for 2 h. Lipopolysaccharide (LPS, 100 ng/mL) was added to the apical side after 2 h for another 1 h and bacterial endotoxin levels were measured in the media of the basolateral compartment.