Blood biochemistry
310600
BLOOD, PATHOLOGY
Blood samples were collected via venepuncture by a trained phlebotomist following an overnight fast of at least 10 h, and analyzed by a commercial pathology service (Monash Health Pathology) Serum 25(OH)D was analyzed using a direct competitive chemiluminescent immunoassay (DiaSorin Inc., USA; cat no: 310600) with level 1 and 2 control coefficients of variation (CV) of 10.7% and 6.5%, respectively (control kit: 310,601). Triglycerides (cat no: 445850) and high-density lipoprotein (HDL) cholesterol (cat no: 650207) were measured using commercial enzymatic assays (Beckman Coulter Inc., USA); triglycerides CV: 4.7 – 7.4% (BIORAD Liquichek control kits: 691 and 692) and HDL CV: 4.4 – 4.5% (BIORAD Liquichek control kits: 691 and 692). Low-density lipoprotein (LDL) concentrations were calculated using the following formula: LDL cholesterol = total cholesterol−HDL−(triglycerides/5) [