Blood sampling and biochemical determinations
BLOOD
Blood samples were collected after a 10 h fasting period at weeks 0, 5, 10 and 15 of the study. Serum was isolated by centrifugation in a Jouan CR-312 centrifuge (Jouan Ltd, Ilkeston, UK) at 1000 g for 15 min and total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides and glucose were analysed by autoanalyzer in fresh serum. Aliquots were stored at – 80 ºC for further fatty acid analysis.