Analysis of RNA-sequencing data
BLOOD
Blood samples for RNA-sequencing were collected using PAXgene Blood RNA tubes (BD biosciences/PreAnalytiX). RNA was extracted from whole blood using PAXgene Blood RNA Kit (Qiagen), and RNA quality was evaluated with TapeStation (Agilent). Sequencing was performed at Macrogen with Illumina 100 bp paired-end reads. Quality and adaptor trimming was done with Trim Galore (v0.5.0) in paired-end mode. Salmon (v0.12.0) was then used for quantification (“quant” mode with --validateMappings) against a human transcriptome index (ensembl release 79). Principal component analysis was run with plotPCA()-function and showed no signs of batch effects (Additional file 