Evaluation of the impact of sample freezing on MBS AST-POCT results
In order to assess the impact of freezing MBS AST-POCT results, analyses were performed only on frozen culture-positive samples analyses, i.e., on samples for which urine culture and AST results were made available by the hospital’s laboratory. Analyses were performed on a total of 10 urine samples. For each sample aliquots (100 μl each) were stored at -80°C and stored up to 7 days. Analyses were performed in duplicate on each sample after 2, 5 and 7 days from collection at the Science Department of Roma Tre University, Rome. Considering the dilution factor, due to the addition of glycerol, 40 μl of the frozen aliquots were analyzed with MBS AST-POCT and results were compared with those previously obtained for the same fresh samples.