Cell transfection
Cells were seed in 12-well plates and incubated with CFD silencing RNA (siRNA CFD) to detect whether CFD impacts Col1a1 production. Fibroblasts were incubated with a final concentration of 100 nM siRNA CFD mixed with Lipofectamine™ 3000 transfection reagent (Thermo Fisher Scientific), Opti-MEM reagent was used for dilution.