2.5.3. Measurements of Salivary CgA Concentrations
CgA
SHIGA
Saliva was collected with a swab and centrifuge tubes. Collected saliva samples were stored at −80 °C until measurement. The concentration of the salivary Chromogranin A (CgA) was determined with a commercially available human CgA EIA kit (Yanaihara Institute Inc., Shizuoka, Japan). The total protein in the saliva samples was measured by the Bradford method with a commercially available dye-assay kit (Takara Bio Inc., Shiga, Japan). The concentration of CgA was corrected with the amount of salivary total protein.