Sample selection
TM3030
Thirty healthy human canines were obtained from the UNOESC Biobank (Joaçaba,
Santa Catarina, Brazil). All samples had straight canals, fully formed roots,
and no curvature or apical resorption. The Research Ethics Committee (CAAE
79772117.0.0000.0121) approved this study. The samples were randomly divided for
scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDS)
(n=15), and microscopic analyses (n=15).Sample preparationThe teeth were sectioned buccolingually with the help of a double-sided diamond
disc (KG Sorensen, São Paulo, Brazil) coupled at a low speed (Kavo, Joinville,
Santa Catarina, Brazil) under constant cooling to obtain two slices per root.
The samples were then washed in running water, and a hemisection (n=15) was used
for structural analysis using low-vacuum SEM and EDS (TM3030, Hitachi, Hefei
Anhui, China). For microscopic evaluation, the samples were identified and
sectioned following the aforementioned protocol. Additionally, one hemisection
was submitted to the RDT protocol while the other, being the control group, was
stored in deionized water for the same amount of time as the RDT.