Mice
Nine-week-old female C57BL/6 mice (n = 70; Taconic, Tornbjerg, Denmark) with a mean weight of 20 gram were housed together in GreenLine type II individually ventilated cages (five mice per cage; Scanbur, Karlslunde, Denmark), in standard laboratory conditions (light/dark cycles of 12/12 h, room temperature of 23.9°C, relative humidity of 22.4% and 20 air changes per hour. Maintenance was performed once a week, and the same individuals handled the animals throughout the experimental period. Mice were acclimatized for one week after arrival. Food and tap water were available ad libitum throughout the acclimatization and trial period. After acclimatization, mice were randomized to different treatment groups. Observers assessing outcomes were blinded to treatment allocation. Treatment groups were mixed before housing to reduce bias. The experiments were carried out following the FELASA guidelines (Federation of European Laboratory Animal Science Associations) and were approved by the Norwegian Animal Research Authority (#24378).