Immunohistochemistry analysis
tumor, Tumor
TUMOR, TUMOR
Tumor samples were fixed with formalin and embedded in paraffin. The tumor samples were provided by the Pathology Department of Shanghai Pulmonary Hospital. Stain the slide with Anti-CISH/CIS antibody-C-terminal (Abcam: ab191447, dilusion:1/100) and Anti-PD-L1 antibody (PD-L1: E1L3, Rabbit mAb, CST: 13684 S, dilusion:1/200). The immunohistochemical (IHC) score was calculated according to the proportion of positive tumor cells (the proportion less than 1% was 1; the proportion from 1%-49% was 2; the proportion larger than 50% was 3) and the average intensity of positive staining (negative staining was 1; weak staining was 2; and strong staining was 3). The range of IHC score was from 1 to 9 for each sample.