Biochemical measurements
TG
INSULIN RESISTANCE, INSULIN SENSITIVITY, INSULIN RESISTANCE
In the early morning, a fasting (12 h) blood sample was drawn. Serum was separated by centrifuging at 3,000 × g for 10 min. Enzymatic colorimetric method was performed to measure FBG, serum concentration of triglyceride (TG) and total cholesterol (TC) (Pars Azmoon, Tehran, Iran). Similarly, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) were measured after blocking other cholesterol containing components by photometric methods. Glycosylated hemoglobin (HbA1c) was assessed using ion exchange chromatography method. Fasting serum insulin was measured by enzyme-linked immunosorbent assay (ELISA) (Monobind Inc,Costa Mesa, CA, USA). To estimate insulin resistance, Quantitative insulin sensitivity check index (QUICKI) and homeostatic Model Assessment for Insulin Resistance (HOMA) were calculated by following formulas [