Enhanced immune response driven by optimized MAPK inhibition
tumor
TUMOR
We therefore hypothesized that the degree of MAPK inhibition achieved in tumor cells might be directly related to the degree of tumor-intrinsic induction of immune gene expression. Accordingly, we evaluated the effects of BRAF/MAPK pathway inhibition alone in patients with BRAFTo evaluate the potential relationship between MAPK inhibition and immune program induction specifically within tumor cells, we utilized patient-derived organoid models successfully generated from baseline tumor biopsies from 10 patients, including 5 with PFS > 6 months and 5 with PFS < 6 months. Organoids were treated with DT and gene expression was measured by quantitative polymerase chain reaction (qPCR). Organoids derived from patients with PFS > 6 months showed greater increases in the expression of genes involved in IFN response (