Quantification of Genomic DNA Expression with Droplet Digital PCR (ddPCR)
STERILE
Paper points were placed into 300 µL sterile PBS and incubated overnight at 4° C with gentle shaking. Bacterial DNA was extracted using High Pure PCR Template Preparation Kit (Roche-Applied Science, Bavaria, Germany) following the manufacturer’s instructions. The genomic DNA quantification was performed with digital quantitative PCR using specific primers: 