B cell phenotype and proliferation.
PROLIFERATION
To discriminate the different B cell subpopulations, thawed PBMCs were stained with fluorochrome-conjugated monoclonal antibodies (see To evaluate B cell proliferation, PBMCs were loaded with 10 μM CellTrace CFSE (Thermo Fisher Scientific) to track divided cells and then cultured in complete medium at a concentration of 2.5 × 10Serum IgG, IgA, and IgM were measured as part of routine analysis.