Plasma and urine samples extraction
An aliquot of 50 µL of plasma or urine was placed in a 1.5 mL of polypropylene centrifuge micro-tube. Protein was removed from the sample by adding 200 µL of methanol (containing 50.00 ng/mL of IS). The mixture was vortexed for 10 min and was then centrifuged at 12,000 rpm, 4 °C for 10 min. The supernatant was filtered through a 0.2-µM PVDF membrane (Chrom Tech) and transferred into a glass insert in a vial before LC‑MS/MS analysis.