4.7. Intestinal Organoid Culture
non-celiac
Samples from duodenal biopsies from the Tampere University Hospital (non-celiac patients). Isolation and culture of human epithelial organoids collected from the small intestinal mucosa were carried out as described elsewhere [The organoids were studied as an in vitro model to support our finding that ZED1227 accumulates on the surface of the epithelial cells and that ZED1227 can inhibit TG2 there. They were maintained as 3D Matrigel-embedded cultures and transformed to 2D monolayers for in-situ staining as described [