tumour, Cancer
BLOOD, TUMOUR, DISEASE PROGRESSION, CANCER
Blood samples were collected at baseline, during radiotherapy, at every evaluation time point and at disease progression. Three 10 mL‐EDTA tubes of peripheral blood were taken, and plasma was separated by centrifugation at 1000 To confirm that genetic alterations detected in blood originated from tumour tissue, we did a genomic analysis of formalin‐fixed paraffin‐embedded (FFPE) tissue samples collected before the start of study treatment or at the time of diagnosis if no dedicated pre‐treatment biopsy was available. Targeted libraries were prepared on the Ion Chef from 20 ng of tumour DNA with the Ion AmpliSeq™Cancer Hotspot Panel v2, quantified with qPCR, amplified and loaded on Ion 540 chips by Ion Chef and sequenced on the Ion GeneStudio™ S5 system (Thermo Fisher Scientific). Output data were analyzed using the Ion Reporter software (Thermo Fisher Scientific).