SARS-CoV-2 genome enrichment
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To create NGS libraries, viral RNA was subjected to reverse transcription and subsequent PCR enrichment. Reverse transcription was performed using random hexamers with the Reverta L Kit (AmpliSens, Russia) following the manufacturer's protocol. Samples (cDNA) were stored at -20°C until amplification. To obtain a near complete genome sequences of SARS-CoV-2 (excluding the 5′ and 3′ ends), a total of 138 primer pairs covering the entire genome were applied according to a previously described multiplex PCR protocol [