4.6. Isolation of Lipoproteins and Quantification
TG
Very low-density lipoproteins (VLDL, d < 1.019 g/mL), LDL (1.019 < d < 1.063), HDLFor each lipoprotein purified individually, the total cholesterol (TC) and TG levels were measured using commercially available kits (cholesterol, T-CHO, and TG, Cleantech TS-S; Wako Pure Chemical, Osaka, Japan). The protein concentrations of the lipoproteins were determined using a Lowry protein assay, as modified by Markwell et al. [