RNA sequencing and data processing
Total RNA from fresh frozen tissues was extracted with TRIzol. Sequencing libraries were generated using a NEBNext Ultra RNA Library Prep Kit for Illumina, and index codes were added to attribute sequences to each sample. The libraries were pooled, and paired-end sequencing (2×150 bp reads) was performed using an Illumina NovaSeq 6000. After RNA sequencing, raw fastq files were trimmed via fastp (v0.20.1)