2.2. Oral Glucose Challenge (OGC)
glucose beverage
BLOOD
Participants arrived in the morning at approximately 6:30 AM in the fasted state and abstained from alcohol, exercise, and caffeine at least 48 h before the study visit. A baseline (0-min) blood sample was collected from a flexible intravenous catheter inserted into the antecubital vein of the left arm. After participants drank a 75 g glucose beverage (Glucola, Mercedes Scientific), blood samples were collected at 15, 30, 60, 90, and 120 min. Blood was collected for the catheter at each time point in an EDTA tube and centrifuged. Plasma glucose was then measured (Analox GL5, Analox Instruments Ltd., Stourbridge, United Kingdom).