TCR sequencing
For TCR sequencing, total RNA was isolated from peripheral blood mononuclear cells (PBMCs) by RNeasy Plus Mini Kit (Qiagen, USA) according to the manufacturer’s instructions. Take3 (BioTek, USA) was applied to determine the final concentration. Total RNA was synthesized into the cDNA library by iRepertoire Short Read iR-Profile Reagent System HTBI-vc. Sequencing was performed by NextSeq 550AR platform with 150-bp paired-end reads. Fastq reads were trimmed based on their low-quality 3′ ends bases. Trimmed pair-end reads were integrated according to overlapping alignment with the modified Needleman-Wunsch algorithm. MiXCR (v2.1.10)