Blood sampling and analyses
BLOOD, STERILE
Blood samples were collected into tubes containing clotting activator (Sarstedt, Germany) and left at room temperature for 15 min before being centrifuged at 4000 × g for 10 min at 4 °C. Serum was aliquoted in duplicate into sterile tubes, placed on dry ice, and stored at − 80 °C. Serum glucose, triglycerides (TAG), glycerol, non-esterified fatty acids (NEFA), lactate, beta-hydroxybutyrate (βOHB), total cholesterol, high-density lipoprotein cholesterol (HDL-c), and low-density lipoprotein cholesterol (LDL-c) concentrations were measured using an automated analyser (RX Daytona, Randox Laboratories, UK). Reported TAG values in the present paper have been blanked for glycerol based on recommendations for clinical research [