Determination of YFV RNA in serum
YFV
YFV-specific real-time PCR was used to determine the viral RNA in serum of study participants. RNA was isolated from 150 μL of serum using a NucleoSpin RNA Virus Kit (Machery-Nagel). One step real-time PCR was performed using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), a FAM-TAMRA-labeled probe and primers (Fisher Biotechnology) according to the manufacturer’s instructions. The primers and probe were all specific for the highly conserved NS5 gene of YFV [