Electrochemiluminescent multiplex assay
TUMOUR NECROSIS
The V-PLEX Human Cytokine 30-Plex Kit (Meso Scale Diagnostics, Rockville, Maryland, USA) was used to assay interleukin (IL)-1α, IL-6, IL-8, tumour necrosis factor (TNF)-α, MCP-1, and macrophage inflammatory protein (MIP)-1β in BAL samples following the manufacturer’s protocol. See Ryu et al., 2020 for detailed methods 