2.6. DNA extraction and sequencing
LYSED
For microbiome analysis, fecal samples were collected at baseline before dosing and final visit in fecal collection Tube DNA/RNA Shield™ (Zymo Research, USA) and stored at −80°C until use. The fecal sample (approximately 500 μL) was lysed with lysozyme (Sigma # L6876), followed by Proteinase K and RNase treatment to remove proteins and RNA. The genomic DNA was extracted from each frozen fecal sample using the Qiagen DNeasy column (Qiagen, USA) (#69506) and was quantified using Nanodrop2000 (Thermo Fisher Scientific, USA).