Secretion of VEGF as a potency assay
Human CD34Quantification of VEGF in the supernatant of ProtheraCytes cultures as a potency assay. (We then conducted an in vitro tube formation assay with HUVECs to determine if the ProtheraCytes supernatant containing VEGF has angiogenic activity. We quantified tube formation in HUVECs that had been cocultured for 6 h with ProtheraCytes supernatant or StemFeed medium as negative control and observed significantly higher tube formation in HUVECs cocultured with ProtheraCytes supernatant (p = 0.0082) (Fig. 