GAD autoantibodies and IgG subclasses
GAD autoantibodies
Serum GAD autoantibodies (GADA) levels were assessed by means of enzyme-linked immunosorbent assay (ELISA) by Synlab Pharma Institute (Munich, Germany).GADA IgG 1, 2, 3, and 4 subclasses were measured by radio-binding assays using IgG subclass-specific biotin-labelled mouse-anti-human monoclonal antibodies bound on Streptavidin Sepharose High Performance beads (GE Healthcare Life Sciences, Freiburg, Germany) (