2.9. Targeted Metabolomic Analysis of Blood
BLOOD
In related to the condition of blood collection, the fasting states blood tests were assigned on the 1st day morning of the meso-cycle, which in this case were on the Mondays of the 1st & 9th week. In addition, training sessions were proceeded on Mondays through Saturdays weekly, and each Sunday was a designated resting day regardless of the weekly training load. Thus, choosing Monday as the blood-collecting day allows us to avoid possible errors due to training-related acute physiological responses. Blood samples were collected into 1 mL centrifuge tubes containing EDTA for plasma. The analytical instrument for this experiment was an LC-MS QTRAP 6500+ (SCIEX). The samples were analyzed in both positive and negative ion modes using a spray voltage of 4.5 kV and a capillary temperature of 350 °C. The mass scanning range was set at 50–1500 