Viral plaque assay and vaccinia virus neutralization assay
tumor, virus infection
TUMOR, VIRUS INFECTION, PLAQUE
Viral plaque assays were performed on body fluid samples (blood, sputum, urine, pleural fluid) collected from patients immediately before and 24, 48, 72, and 96 hours after Olvi-vec treatment to assess for the presence of viral particles. Post-treatment tumor biopsies collected 2-5 days after treatment also underwent assessment for viral particles using viral plaque assays. In brief, patient samples were plated on confluent layers of CV-1 cells. Evaluation of virus infection was done by visual assessment of viral plaque in wells with both CV-1 cells and patient samples. Additionally, post-treatment serum samples obtained from patients 60 days after Olvi-vec treatment were assessed for the presence of Olvi-vec neutralizing antibodies 