3.3. Alpha diversity analysis
GROUP B
The values of Good coverage of all libraries were over 99.9%, indicating that the sample sequencing was relatively complete. Moreover, no significant differences were observed in the evaluated alpha diversity indexes (Shannon, Simpson, Chao1) between the groups. Rarefaction curves indicated that the sequencing depth was enough since the samples reached the plateau phase (Fig. The graphical representation shows the rarefaction curve of each sample. The X-axis shows the effective sequences per sample. Y-axis shows the observed OTUs per sample. Aa12–Aa61 represent the samples’ names before intervention. Ab12–Ab61 indicate the samples’ names after the intervention. In the legend, Aa and Ac represent the total samples of Group A before and after the intervention, respectively. Ba and Bc represent the samples of Group B before and after the intervention. The legends of the later figures indicating the curve names are the same, respectively. OTU = operational taxonomic unit.