DISCUSSION
postoperative pain, Postoperative pain, pain, MARF, bone dehiscence, Inflammation, LSCC
INFLAMMATION, ALMEIDA
A gingival width of 2 mm is sufficient to maintain healthy gingiva (Wennstrom & Lindhe, The apical repositioning flap introduced by Friedman increases the risk of bone resorption (Lang & Löe, In a 1‐ to 11‐year follow‐up study, Carnio et al. (The MARF and PRF groups resulted in a significant increase in keratinized tissue. MARF is an effective and efficient method to increase keratinized tissue and the attached gingival width. The main limitation of this technique is the presence of at least 0.5 mm of attached gingiva before surgery so that the wound is surrounded by keratinized tissue. The presence of bone dehiscence is another contraindication (Swarna et al., Alaood et al. (Free gingival grafting is the most common method for increasing keratinized tissue and attached gingiva (Miller, Temmerman et al. (Postoperative pain was also slightly reported in both groups, with no significant difference. Inflammation peak and maximum postoperative pain occur within the first 24 h following surgery. Since the PRF membrane acts as a biological dressing, it was expected to be effective in reducing postoperative pain. Several studies demonstrate that PRF decreases postoperative pain (de Almeida Barros Mourão et al., Another possible reason is using silica‐coated blood collection tubes. Silica in the tube causes negative effects on periosteal cells (Masuki et al., In the PRF group, wound shrinkage was significantly lower than in the MARF group. These values were reported to be 30.9% for the PRF group and 51.1% for the MARF group. PRF group shrinkage values are comparable to those of Temmerman et al. (According to the shrinkage results, for obtaining at least 2 mm of new keratinized tissue, we suggest a 4 mm apically repositioned flap in MARF and a 3 mm repositioned flap in the MARF + PRF method.Recent advances in the field of platelet derivatives lead to the use of low‐speed centrifugation concept (LSCC) and horizontal centrifugation. LSCC results in an increased growth factor concentration. Therefore, enhancing the growth factor concentrations improves the potential of tissue regeneration and wound healing capacity of the fluid PRF‐based matrices (Choukroun & Ghanaati, Due to recent improvements in platelet derivatives, further clinical studies are necessary to evaluate LSCC and horizontal centrifugation to enhance clinical results.