2.2. Participants
hypermobility, pain
NEUROLOGICAL DISORDERS
Participants were recruited from the university community. Ninety healthy volunteers were initially screened and ten were excluded using different exclusion criteria (A pilot study was conducted in order to calculate the sample size. The variable used for the sample size calculation was the improvement in hamstring muscle flexibility. In a previous pilot study carried out in our population, the standard deviation around the mean hamstring muscle flexibility was 10.3 mm and the difference was 6.5 mm at baseline. Using this data, 40 patients per extremity were needed for a 5% confidence level and 80% statistical power.The inclusion criteria stated that the subject needed to be over 18 years old. The exclusion criteria consisted of hypermobility, hamstring injury in the previous 6 months, diagnosed orthopedic problems or surgery in the lower limbs, back pain or spine surgery, and systemic or neurological disorders.Interventions and measurements were carried out by the same three blinded therapists, two of which controlled the intervention, with the other taking measurements and recording data. To minimize bias, participants could not see and were not informed of any result obtained during measurement.The same conditions, such as the temperature, equipment, and time of day, were used in all the cases.After baseline examination, patients were randomly assigned to one of two groups: PNF or FR. The allocation sequence was determined before the study using a computer-generated randomization list (Ramdom.org).