RNA-Seq.
Whole blood RNA-Seq was performed using paired-end RNA-Seq. Peripheral blood was collected into Tempus RNA stabilization reagent (Life Technologies, Thermo Fisher Scientific). Total RNA was extracted from all samples using the Tempus Spin RNA Isolation kit (Life Technologies, Thermo Fisher Scientific). Quality control was carried out by measuring the RNA concentration of each library (mean RNA concentration, 109.56 ng/μL; median RNA concentration, 106.05 ng/μL; 