TUNEL assay results on cell apoptosis in wound granulation tissue
On Day 14 post‐surgery, samples from the Zibai ointment group showed signs of apoptosis (brown‐yellow cells with nuclei) under light microscopy, including chromatin condensation (arrangement close to the karyotheca in a hemisphere‐shaped, crescent‐shaped or sickle‐shaped manner), cytoplasmic pyknosis, reduced cell volume, loose cell arrangement, irregular‐shaped cells, and occasional apoptotic bodies. The apoptotic cells in the petroleum jelly group decreased significantly at the same time point. We did not find any significant differences in the proportion of positive cells between the Zibai ointment and petroleum jelly control groups on Days 7 and 21 postsurgery (Apoptosis in each group detected by TUNEL assay (×200).Apoptosis index of in situ end‐labeling (The number of apoptotic positive cells in the Zibai ointment group on Day 7 after operation, compared with the Vaseline group, The number of apoptotic positive cells in the Zibai ointment group on Day 14 after operation, compared with the Vaseline group, The number of apoptotic positive cells in the Zibai ointment group on Day 21 after operation, compared with the Vaseline group, 