Pharmacokinetics (PK)
BLOOD
Blood sampling for PK was performed before beginning infusion and at 15 min, and 30 min post-beginning infusion, and at 30 min, 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h post-infusion on days 1 and 2, and at 30 min post-beginning infusion, and at 1 h, 2 h, 4 h, and 24 h post-infusion on days 3 and 4, and then at 24 h (day 5) and 48 h (day 6) post the last infusion. Urine was collected before treatment and after 6, 12, and 24 h on day 1. Urine collection was then performed every 24 h until 48 h after the last infusion (day 6).LNA-i-miR-221 concentrations were assessed by a previously developed and validated mass spectrometry analytical method [