MDCF-2 effects on MAG gene expression
Microbial RNA-sequencing (RNA-seq) analysis was performed using RNA isolated from faecal samples collected from all of the study participants just before initiation of treatment, and after 1 month and 3 months of treatment (We used principal component analysis (PCA) to determine baseline differences in overall (DNA-based) MAG abundance profiles, or the abundance of MAG-derived RNAs in the expressed metatranscriptomes, between the treatment groups, and to subsequently identify microorganisms that were the principal drivers of shifts during treatment. Figure 