Stool collection and microbiome analysis
STERILE
Stool was collected up to 24-hours prior to the visit or during visit 2, 3, 4, 5, or 6. Stool collected at home was stored in a sterile plastic vial at 4°C and processed immediately after receiving at NIH CC. Samples were flash frozen with liquid nitrogen and transferred to -80°C and stored until further analysis for microbiome sequencing and run in one batch. Fecal DNA was isolated for microbial compositional analysis using PowerSoil