Handling and biochemical analyses of blood samples
Peripheral and liver vein blood samples
Peripheral and liver vein blood samples were immediately stored on ice until centrifugation at 4°C. After allocation in cryotubes, plasma samples were transported on dry ice and stored at −80 °C until analysis. Plasma concentrations of glucagon were analyzed using ELISA (Mercodia Glucagon ELISA, Mercodia AB) (Wewer Albrechtsen et al., 