Procedures
amenorrhea, diabetic, Diabetes, low blood glucose, Hypoglycemia
BLOOD, HYPOGLYCEMIA, DIABETES
After an 8–12 hour overnight fast, all laboratory tests were conducted on participants between 6:30 AM and 9:30 AM. All subjects had a standard 75-g oral glucose tolerance test (OGTT) at the start and end of the trial. A baseline blood sample was collected before administering a 75-g oral glucose load. Blood was then drawn 30, 60, and 120 minutes later for assessment of the levels of glucose and insulin. Glucose tolerance can be classified as normal, impaired, or diabetic based on the American Diabetes Association. Hypoglycemia was considered as signs of low blood glucose that were validated by a self-monitored blood glucose measurement of less than 3.9 mmol/L (All individuals received standard anthropometric data referring to height, body weight, waist circumference(WC), and waist-to-hip ratio(WHR) taken by a trained physician at baseline and the 12Transvaginal three-dimensional ultrasound and transabdominal ultrasound which is only performed in nonsexually active females were carried out to measure the antral follicle count(AFC) and ovarian volume in the early follicular phase or the amenorrhea period at baseline and study completion by a single investigator of the ultrasonic department, Hospital of Chengdu University of Traditional Chinese Medicine. The volume of each ovary was calculated as follows: length*width*height*0.523 (