ELISPOT for characterization of CD8+ and CD4+ T memory cells functionality
Elispot assays were performed using the Human IFN‐γ ELISpot PLUS (HRP) kit (MabTech) with precoated plates. Thawed PBMCs were diluted in RPMI 1640 medium containing 10% foetal bovine serum (FBS; Biowest) and incubated for 24 h at 37°C in humidified 5% CO2 atmosphere. After 24 h, cells were counted and seeded (25 × 10