Cell lines and cell viability assays
MUTANT
All mutant Ba/F3 cell lines were obtained from the KYinno BIOTECHNOLOGY (Cell viability was determined using the Cell Counting Kit-8 assay (DOJINDO). Cells were collected from suspension medium, spun down at 300 g for 5 min and resuspended in fresh RPMI medium. 3000 cells per well were plated in 96-well plates. Cells were treated with ten different concentrations of inhibitors in serial threefold-diluted inhibitors or vehicle alone for a final volume of 100 μl per well. After 72 h, 10 μl of Cell Counting Kit-8 was added to each well. IC50 values were calculated using GraphPad Prism 9 at 50% inhibition. Each experiment was replicated three separate times.