MATERIAL AND METHODS
This parallel-group randomized clinical trial, multicenter study with a 1:1 allocation ratio, registered under the protocol ID NCT03881813 (https://clinicaltrials.gov/), according to the CONSORT statement of the updated guidelines for reporting randomized clinical trials, was approved by the Institutional Review Board of the university (Ref:IRB-941/DUHS/Approval/2017/162). The study was conducted at the department of Orthodontics, Dr. Ishrat Ul Ebad Khan Institute of Oral Health Sciences (DIKIOHS) and Dow Dental College (DDC), in Dow University of Health Sciences (DUHS), from November 2017 to March 2019.The sample size was calculated with PASS (v.11) using two sample proportion with 95% confidence interval and 80% power of test, based on the values for measured clinical indices obtained from a previous article with estimated population size of 60 patients in six months
