RESULTS
DEGENERATE
The flow of study participants can be seen in Demographic parameters of the elegible couples.Values are expressed as mean ± standard deviation and in
parentheses the 95 % confidence interval.T test (5 % significance).D3= participants of group with embryonic culture for three daysD5 = participants of group with embryonic culture for five days.The general results of the study can be seen in Comparison of IVC and IVF technique results between the D3 and D5 groups.The 95 % confidence intervals were computed by modified Wald method.
Cleavage rate (%) = Total of cleaved embryos/Total retrieval oocytes
post culture, except degenerate. Blastocyst formation rate (%) = Total
of formed blastocysts/Total of cleaved embryos. Blocked embryo rate (%)
= Total embryos that did not reach blastocyst/Total of cleaved embryos.
IVC = Intravaginal Culture, IVF = In vitro Fertilization, D3= embryonic
culture for three days, D5 = embryonic culture for five days.One limitation of our study was the lack of evaluation of the fertilization rates due
to the unavailability of the device and the need to maintain the incubator
environment using the IVF technique. The incubator used for the IVF group remained
unopened to prevent interference such as exposure to light and variations in
temperature, which could interfere with the results.There was no significant difference for the cleavage rate, in Group D5 79% (IVC) and
83% (IVF); in Group D3 75% (IVC) and 53% (IVF). In the blastocyst formation rate,
Group D5 presented 46.7% (IVC) and 40% (IVF). The embryos blocked in Group D5 were
53.3% and 60% for IVC and IVF, respectively (The analysis of the quality of the embryos recovered in Group D3 showed that the
embryos from IVF showed a better synchrony in the number and quality of the
blastomeres (
Embryonic classification of the D3 Group in two scores, 2 to 7 cells
(Black) or 8 to 12 cells (Gray). Embryos IVC culture (n=15), embryos IVF
culture (n=10). IVC = Intravaginal Culture, IVF = When comparing the results found in Group D5 in both techniques, based on the
cleavage rate, the result shows an equivalence in the formation of a blastocyst
(
Blastocyst classification according to degree of expansion of the
blastocele, of the D5 Group. Embryos IVC culture (n=7), embryos IVF
culture (n=6), IVC = Intravaginal Culture, IVF = The measurement of the pH of the medium contained in the INVOCell™ device in
both Groups D5 and D3 showed no differences (D5 7.26±0.05 and D3
7.25±0.07, 