Clinical microbiology and speciation
Biopsies collected at days 0 and 7 were sent to the IU Clinical Pathology Laboratory for standard bacterial culture-based analysis on tryptic soy agar. Bacterial load was evaluated using a standard semiquantitative quadrant streak method where 0 represented no colony/growth, 1 = growth on quadrant 1 (+), 2 = growth on quadrants 1 and 2 (++), 3 = growth on quadrants 1–3 (+++), and 4 = growth on all four quadrants (++++). Bacterial speciation was performed by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF) using an MALDI biotyper