Statistical analysis
inflammation, PWH, A5331
REGRESSION, HEAT, INFLAMMATION
A total of 50 unique lipid mediators were measured in plasma samples, with 42 SPMs and 8 pro-inflammatory lipid mediators (The first objective of this study was to compare plasma levels of i) lipid mediators and ii) metabolomes between PWH (pre-intervention) and SN individuals. To visually examine how mediators/metabolomes and these two populations cluster together, hierarchical clustering heat maps were created for mediators/metabolomes (The second objective of this study was to determine the association of lipid mediators of inflammation with the monocyte activation marker sCD14 among PWH (i.e. A5331 study participants at baseline). Logistic regression was used to determine the association of high inflammation (outcome variable of “high” sCD14 defined as the highest quartile (Q4) compared to Q1–Q3) with i) individual metabolome, ii) individual principal components (derived from unsupervised principal components analysis) of the metabolome, and iii) individual principal components of the mediators (further details in The final objective of this study was to study whether and how the administration of aspirin changed levels of lipid mediators among PWH (i.e. among A5331 participants pre- and post-intervention). We conducted analysis of variance (ANOVA) to assess the effect of each aspirin arm in A5331 (100 mg and 300 mg arms) relative to placebo on 12-week change (i.e. change in lipid mediators/metabolomes from baseline to 12 weeks) in mediators and metabolomes (further details in With the exception of analyses utilizing FDR-adjusted p-values, all other exploratory analyses used unadjusted p-values conservatively assessed at the 1% significance level to limit the number of false positive findings.