Fertilization by intravaginal culture with the invocell™
device
CAVITY
The cumulus oophorus complex were inseminated in the INVOCell™ device
following the manufacturer’s recommendation, which is to use continuous culture
medium supplemented with 10 % synthetic serum (Ingamed®) and a
concentration of 40.000 sperm recovered/ml, with a volume of 1000 µL. The
device was subsequently inserted into the vaginal cavity with the retaining
diaphragm to maintain its position. The withdrawal occurred on the
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