In vivo hydroxychloroquine suppressed IFNα secretion following TLR7 stimulation, but not after TLR3, TLR9, or RIG-I-like receptor stimulation
SD
To study the effects of HCQ on TLR/RIG-I-mediated IRF activation, PBMCs were stimulated with different endosomal TLR ligands: poly I:C (TLR3), imiquimod (TLR7), CpG class A (TLR9), and poly I:C lyovec (RIG-I). Overall, no HCQ effect was observed on IFNα responses (Fig. In vivo HCQ inhibited IMQ-induced IFNα release, but not TLR3, TLR9, and RIG-I. PBMCs were stimulated with 50 μg/mL poly I:C (TLR3), 1 μg/mL IMQ (TLR7), 2.5 μM CpG A (TLR9), or 1 μg/mL poly I:C/lyovec (RIG-I) at 0, 12, 24, 48, 72, and 92 h after primary HCQ dosing. IFNα release was measured by ELISA. Data is shown as mean + SD as one-sided error bars. Dotted vertical lines indicate HCQ dosing times