2.1. Participants
sleep disorder, anxiety, gynecological malignancies, major somatic disease, depression, substance abuse, diabetes
IRREGULAR MENSTRUATION, BREAST TUMORS, GYNECOLOGICAL DISEASE, HYPERTENSION, DISEASES, DIABETES
100 healthy perimenopausal women were enrolled originally from the outpatient department of the Ningbo Women and Children Hospital. All participants were 45–55 years Chinese women. They should experience (1) climacteric symptoms, including hot flashes, sleep disorder, anxiety, and depression, for less than 1 year and (2) irregular menstruation and have at least one menstruation in the past 6 months. The participants would be excluded if they conformed to one of these criteria: diagnosis of cardiovascular endocrine and gynecological diseases (hypertension, diabetes, gynecological malignancies, breast tumors, and other diseases), major somatic disease, substance abuse, current use or discontinuation of health care drugs, or reproductive hormone treatment for less than one month. Then, all participants were grouped into study and control groups in a random way (each 