Quantification of folate forms
Human milk folate
Human milk folate forms (nmol/L), including UMFA, 5-MTHF, tetrahydrofolate (THF), 5,10-methenylTHF, 5-formylTHF, and 4α-hydroxy-5-MTHF (MeFox; oxidation product of 5-MTHF), and maternal plasma UMFA (nmol/L) and plasma 5-MTHF (nmol/L), were determined via liquid chromatography-tandem mass spectrometry (LC-MS/MS); detailed description of quantification methods are published elsewhere