4.8. UHPLC-HRMS-Based Metabolomics
The sample preparation for the UHPLC-HRMS/MS-based metabolomics was carried out according to previous studies [Bucket generation was performed with the following parameters: S/N threshold = 2; correlation coefficient threshold = 0.2; minimum compound length = 10 spectra; smoothing width = 1. All features detected by the UHPLC-HRMS/MS were subjected to data processing consisting of the inclusion of features based on values greater than 5% from blank samples, a coefficient of variation (CV%) of QCs samples (mean of replicates) lower than 20%, and the quantity of missing data being lower than 10% in experimental samples [Data Analysis v4.2 (Bruker Daltonics) was used for the identification of the fragment ions (MS/MS) of those detected compounds, which were further putatively annotated by comparing their exact mass (error ≤ 10 ppm) and fragment ions with those data in the HMDB (