Candidate genes confirmed by qRT–PCR
cancer, S cancer
CANCER
We chose four genes (CCL20, CXCR1, CXCL8, and TNFAIP3) for validation. To better reflect the differences between P normal (Pn), P cancer (Pc), S normal (Sn) and S cancer (Sc), we calculated the ratio of the normal samples to the cancer samples (Pn/Pc and Sn/Sc). On qRT–PCR, three genes (CXCR1, CXCL8, and TNFAIP3) were consistent with the RNA-seq results and were significantly different between P and S (Fig. Verification of gene expression in qRT–PCR experiments. Data were expressed as mean ± SE. Significance level was indicated as 