Immunological analysis
REGRESSION, DISEASE
To examine whether clinical efficacy could be linked to immunological response, we assessed the association between clinical response and the number of HPV-16 E7–specific, IFN-γ–producing cells (spot-forming cells numbers) among peripheral blood monocytes at the study endpoint. The measured spot-forming cells among peripheral blood monocytes collected at the study endpoint increased in parallel to clinical disease regression status (stable disease, partial response, and complete response; analysis of variance test, Immunological responses and clinical efficacy. To evaluate the magnitude of human papillomavirus (HPV)-16 E7–specific Th1 immune response, the numbers of HPV-16 E7–specific interferon-γ–producing cells in peripheral blood were obtained by enzyme-linked immunosorbent spot assay. The spot-forming cell numbers (spot-forming cells per 10^6 peripheral blood monocytes) for each patient were plotted on the 