Infusion studies in humans
We obtained approval by the local ethics committee and written consent from study participants for all procedures prior to the experiment. 11 healthy men without any medications underwent an overnight fast, after which we catheterized the large antecubital veins of both arms to allow for separate infusion and sampling access. After a rest period of at least 60 minutes, human ANP was infused for 135 minutes at a maximum rate of 25 ng/kg/min as previously described (