Sample size {14}
For sample size estimation and power calculation, we analyzed untouched saliva samples of a previous sleep deprivation study, in which participants underwent 40 h of prolonged wakefulness and a control condition including 8 h of sleep in random, crossover fashion [For comparison, relevant completed trials with comparable protocols had sample sizes between 10 and 16 (10 male, 12 male, 12 female, and 16 (eight men)) [