2.6. Statistical Analysis
The Shapiro–Wilk test was applied to evaluate the normality of the data. The required sample size was considered an average statistical power equal to or higher than 0.80 for the main outcome variable VOIn addition, the effect size (ES) was used to calculate the magnitude of the differences. The ES was computed as Cohen’s d (t-test) and f (ANOVA) statistics and interpreted as following: trivial (0–0.2), small (0.2–0.6), moderate (0.6–1.2), large (1.2–2.0), and very large (2.0–4.0) [For the comparison between cardiovascular responses and metabolic variables before and after training, Two-way ANOVA for repeated measurements in the time factor with Sidak’s post hoc was used. The level of significance of α ≤ 0.05 was adopted. The descriptive statistics were mean and standard deviation. The software used was GraphPad Prism version 8.0 (GraphPad Software Inc, Boston, MA, USA).