Organoid basal growth media
Organoid basal growth media consist of 30% DMEM/F12 media supplemented with 20% FBS, 50% WNT3A conditioned media, 20% R-spondin conditioned media, 1× B27 (Life Technologies; 17504-044), 1; N2 (Life Technologies; 17502-048), 10 mM nicotinamide (Sigma; N0636), 1.25 mM 