Sample collection and DNA/RNA preparation
tumor
BLOOD, TUMOR
All tumor tissues were obtained from paraffin sections retained after surgery (patients 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11). Blood samples were collected and mixed with an EDTA-based anticoagulant. The extraction of DNA and RNA was performed from the paraffin sections of the postoperative tumor tissues with the All Prep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA/Illumina, San Diego, CA, USA); DNA from EDTA-anti coagulated peripheral blood samples was extracted with the QIAamp DNA Blood Mini Kit (Qiagen).