Microbiological analysis
The DNA Extraction Procedure was carried out using the Modified Proteinase-K method, and the isolated DNA was stored at -20Forward primer: AGG CAG CTT GCC ATA CTG CGReverse primer: ACT GTT AGC AAC TAC CGA TGTThe RT-qPCR amplification and detection were performed with the Realplex master cycler (Eppendorf) using a 96-well format. To limit contamination, the reactions were set up in a laminar airflow chamber (Bio-safety cabinet), and run and analyzed in another laboratory, where DNA manipulation was not performed. PCR reactions were carried out in a total volume of 20μl containing 2μl of template DNA, 10μl of Quantitect SYBR green PCR master mix (Qiagen, JJ Biotech, India), and 8pm/μl of each of the 