Preparation of bimatoprost gel
The first step was to dissolve 1% carbopol in distilled water (2 g is dissolved in 100 ml of distilled water under stirring conditions), the second step was to activate carbopol gel formation by using 0.01 gm or 0.03 gm of bimatoprost for every 100 ml of gel, and the third step was to incorporate the gel preparation into the base with 5 min of continuous stirring and stirring to obtain a homogeneous clear drug–gel solution.