Emergence of T cell responses to TYRP1 and other melanoma antigens
CD8+ T cells and CD14+ monocytes were isolated from PBMCs. The monocytes were cultured with IL-4 and GM-CSF to produce DCs, which were then transfected with plasmids (1µg/10ELIspot Data. CD8+ T cells and dendritic cells (DCs) were isolated from peripheral blood mononuclear cells (PBMCs). The DCs were then transfected with plasmids driving expression of OVA (positive control), TYRP1 (vaccine antigen), gp100 (another melanocyte differentiation antigen), or nothing (negative control); then cultured with purified CD8+ T cells 