IHC and immunofluorescence staining.
SKIN LESIONS
Slides of skin lesions before anti–PD-1 treatment were subjected to IHC or immunofluorescence staining with antibodies against PKCθ (Cell Signaling Technology, 13643), p-PKCθ (BD Biosciences, 612735), CD3 (Abcam, ab699), and PD-1 (Abcam, 137132).