Blood samples
hypoglycemia, fasting blood glucose, TG
HYPOGLYCEMIA, BLOOD
Venous blood was drawn at 5:30 am after fasting for 12 h. Blood samples were sent to the laboratory within an hour. The serum levels of fasting blood glucose (FBG), total cholesterol (TC), TG, low density lipoprotein (LDL) cholesterol, and high density lipoprotein cholesterol (HDL) were measured using the standard enzymatic procedure (Hitachi LABOSPECT 008 AS, Japan).Non-fasting TG was taken 120 min after eating 100 g of standard carbohydrate provided by China Foods Limited (Beijing, China) (The commercial Roche test on the Cobas 8000/801 automatic analyzer (Roche Diagnostics, Mannheim, Germany) was used to measure fasting insulin (FI) by electrochemiluminescence (ECL). HOMA-IR was calculated by the formula HOMA-IR = FBG (mmol/L) × FI (mIU/L)/22.5 (LBGI was used to evaluate the frequency and degree of hypoglycemia based on the mathematical processing of BG measurements. The specific calculation methods were as follows:1) The BG value was transformed:
2) The risk value for BG was calculated according to N is the total number of blood glucose measurements; rl is the risk of hypoglycemia (ADAs of beinaglutide were measured by the electrochemiluminescence method, which is based on beinaglutide labeled with biotin and metal ruthenium, using the classic bridging principle to detect ADAs against beinaglutide in human serum. The ruthenium in the complex “biotin-labeled beinaglutide–ADAs–ruthenium-labeled beinaglutide” excites a light signal value at 620 nm and is captured by an MSD (Meso Scale Discovery) instrument. The captured signal intensity is proportional to the titer of ADAs.