PCR of Ad5-yCD/
ADENOVIRUS, BLOOD
Blood was obtained before the adenovirus injection and at least once a week after for semi-quantitative determination of adenoviral DNA in blood. Total genomic DNA was isolated from 2.5–3 mL of blood using Qiagen columns following the protocols recommended by the manufacturer (QIAGEN GmbH, Hilden, Germany). 2–3 μL of eluted DNA was used as a template for the PCR reactions. The PCR primers have been previously described [