Whole-exome sequencing and read alignment
tumor
BLOOD, TUMOR
WES was implemented on the formalin-fixed paraffin-embedded tumor tissue and matched peripheral blood samples. GeneRead DNA FFPE Tissue Kit (QIAGEN, GER) was employed for FFPE section extraction, while Mag-Bind® Blood & Tissue DNA HDQ 96 kit (OMEGA) was utilized for blood sample extraction. The dsDNA HS Assay Kit (ThermoFisher Scientific, USA) was used for DNA quantification. Sequencing libraries were built by SureSelect XT Human All Exon V6 (Agilent), and sequencing procedures were utilized by the NextSeq 550AR platform with 150-bp paired-end reads. SOAPnuke