Introduction
muscle mass, muscle loss, sarcopenia
SARCOPENIA
Preventing sarcopenia [Both protein intake and physical activity are crucial (among other things) for maintaining and improving muscle mass and when inadequate detrimental muscle loss will follow [In combination, resistance exercises also enhance/prolong the postprandial increments in MPS for up to 48 h [Further, the protein quality (measured as the digestible indispensable amino acid score [Both protein synthetic and muscular contractile processes are energy demanding and assumedly affect the skeletal muscle metabolome. Knowledge on alterations in the metabolome of the skeletal muscle in humans is scarce, due to analytical and technical challenges [This study therefore aims to elucidate whether adaptation to long-term exposure to exercise training and/or enhanced protein intake affects the basal MPS as well as the responsiveness to a single serving of protein in healthy older individuals above 65 years.The setting of the present study was a 12-month intervention with daily supplementation of high- or low-quality protein and carbohydrate as non-protein control, and two groups on the high-quality protein diet conducting either heavy or light load resistance training. We hypothesized that the basal and protein-stimulated postprandial MPS rates are elevated in the exercise training groups after 12 months of intervention and that the intake of protein of different qualities will not affect the acute muscle protein synthetic response to protein intake. The impact of the 12-month interventions and acute postprandial state on the skeletal muscle metabolome was explored with the purpose of generating hypotheses.