Samples collection
PD
STERILE, STERILE, PLAQUE
Subgingival microbial samples were taken from the maxillary experimental site showing the deepest PD at T0. The same site was then sampled at T1. Prior to microbiological sampling, supragingival plaque was removed with a curette to avoid contamination of the sample with supragingival plaque. Sterile paper points size 30 or larger were inserted to the bottom of the periodontal pocket/sulcus and kept in place for 10 s. The samples were then placed in a sterile microcentrifuge tube and frozen until lab processing.