Analysis of in situ tumor-specific CD8 response in responders
LIVER METASTASES
Among patients with partial response, one patient who underwent resection of remaining liver metastases was monitored using single-cell RNA sequencing (scRNA-seq) and T cell receptor (TCR) sequencing. PBMCs were taken at baseline, at 1 month and at the time of liver surgery. Tumor-infiltrated lymphocytes (TILs) were isolated from liver metastases (Extended Data Fig. 