16S Pacbio sequencing of fecal sample DNA
Fresh fecal samples were collected before and after the treatment for the gut microbial analysis. DNA was extracted from the subjects’ feces using a Power SoilAfter using the SMRT Link tool (version 8.0, provided by Pacbio), then Lima v1.7.0 software to obtain the Barcode-CCS sequence data, UCHIME v8.1 software to remove the chimera sequence, the optimization-CCS sequence was obtained for bioinformatics analysis. Trimmed sequences from each sample were clustered into operational taxonomic units (OTUs) based on a 97% sequence similarity using USEARCH v10.0 method. The taxonomical analysis was performed by alignment with the Bacterial Silva database (Release132, 