Single-cell RNA and TCR sequencing.
SKIN
Skin tissue samples were obtained by 6 mm punch biopsy. After removal of subcutaneous fat, the tissues were chopped and digested with 50 mg/mL Collagenase 1A (Sigma-Aldrich) using a gentleMACS dissociator (Miltenyi Biotec) for 2 hours. After incubation, 1 mg/mL DNase I (Sigma-Aldrich) was added. Dead cells were excluded using the Live/Dead Fixable Near-IR Cell Stain kit (Invitrogen), and single cells were stained with BV421-conjugated anti-CD45 (HI30) and PE-conjugated anti-EpCAM (9C4) (both from BioLegend). Live CD45