4.3. Genetic Determination
After collecting the saliva sample, it was preserved at a temperature of −80 °C until further processing. The extraction of genomic DNA was performed using the E.N.Z.A. forensic DNA kit (Omega Bio-Tek Inc., Norcross, GA, USA), following the guidelines provided by the manufacturer. For this particular study, the samples were obtained from Alicante BioBank and processed following standard operating procedures.The following genetic variants were genotyped at the Molecular Biology Laboratory of ISABIAL (Alicante, Spain), and their results were used to guide opioid treatment: 