Statistical analysis
The sample size was calculated by Power and Sample Size Calculation version 3.0.43 (We used the Shapiro-Wilk test to assess the data normalization. For continuous data, Mean ± standard deviation(SD) or median(interquartile range)were used to describe normal or non-normal distribution respectively. Nonparametric Wilcoxon signed-rank test(non-normal distribution) and paired-sample t-test(normal distribution) were applied to intra-group values comparisons. To compare the baseline values and the change of clinical variables among inter-groups, the Mann–Whitney test or independent-sample t-test were used for non-normal and normal data, respectively. Classified variables were expressed as percentages (frequencies) and compared by the chi-square test or Fisher exact tests. The level of statistical significance was set at p-values of <0.05 and all p-values were two-tailed. All data analyses were conducted using IBM SPSS Statistics version 24.0.