Flow cytometry
PROLIFERATION, EVENTS
The monoclonal antibodies anti-human-CD11b (ICRF44), -CD14 (M5E2), -CD19 (HIB19), -CD56 (B159), -CD45RA (HI100), -CD45RO (UCHL1), -CCR7 (2-L1-A), -CD4 (RPA-T4), -CD8 (HIT8A), -CD25 (M-A251), -CD38 (HB7), -4-1BB (4B4-1), -PD-1 (EH12.1), -CD107a (H4A3) (Beckton Dickinson); CD1a (REA736), -CD83 (REA714) (Miltenyi) were used for immunophenotyping of T cells and/or DCs. T cell proliferation was assessed according to the CFSE fluorescence dilution. The change in proliferation was calculated by normalizing the percentage of proliferated T cells from the co-cultures with the S1-mDC to that of obtained from the non-specific antigen-loaded mDC co-cultures. Median fluorescence intensity (MFI) and percentage values were obtained from the events gated on CD4