Sample collection
non-cancer, cancer
ADVERSE EVENTS, CANCER, DISEASE
Sample donors were trained on how to use the acapella assist device (Smiths Medical, St. Paul, MN), and expel their sample by coughing into a specimen cup, repeating this procedure at home for three consecutive days and storing their specimen cup in a refrigerator. Sample donors did not report experiencing any adverse events related to the sample collection procedure. Within one day after collection was completed, the sample was shipped overnight to the bioAffinity laboratory where further processing and FCM analysis took place by technicians blinded to the origin of the sample as well as the clinical information of the donor.A set of 171 sputum samples was analyzed on the LSRII flow cytometer. One hundred and sixty-eight of the LSRII sample set were used for training and testing the model, as well as for developing the analysis pipeline. The final model was then validated on 150 LSRII samples that passed quality control. A second set of 45 samples was analyzed on the Navios EX. Thirty-two passed quality control and were used to independently measure the generality of the model/analysis pipeline by excluding a possible dependency on a particular flow cytometer or team of researchers. See Fig. Utilization of Sputum Samples. Of the 171 samples run on the LSRII that were originally considered (136 non-cancer; 31 cancer; 4 with unconfirmed health status), 168 samples were used for model building and analysis pipeline development. This included four samples for which we did not have a definitive disease status because the addition of unlabeled samples had been shown to be helpful in model building [This included four samples for which we did not have a definitive disease status because the addition of unlabeled samples had been shown to be helpful in model building [