Spermatozoa collection and preparation
ANEUPLOIDY
For the 1,212 couples undergoing ICSI with PGT-A solely for aneuploidy, semen samples were collected by masturbation after 2–5 days of abstinence and allowed to stand for 15 minutes at 37°C to promote liquefaction. Concentration and motility were analyzed by viewing 5 μL of the semen sample in a Makler chamber. The sample was then combined with media comprised of HEPES-buffered human tubal fluid (H-HTF; Irvine Scientific, CA, USA) supplemented with human serum albumin (HSA solution G Series culture media; Vitrolife, Goteborg, Sweden) and centrifuged at 600 