2.7. SC Obturation with BC-Coated GP
After confirming tug-back and designated sealer application, the apical 2 mm of the master cone was evenly coated and inserted up to the working length. Excess GP was seared off and vertical compaction of coronal GP was completed.Following obturation, all access cavities were sealed with restorative-type GIC (3 M, ESPE). All control group samples were prepared in a similar manner as described above. For positive control, the samples were left unfilled, whereas negative control samples were obturated with Sealapex and the CLC technique. To promote complete sealer setting, all samples were stored in an incubator (Binder GmbH) at 37 °C under 100% humidity for 1 week.