Pharmacokinetic studies
BLOOD
According to the preclinical animal data, the time points for the collection of single-dose and multiple-dose PK blood samples were as follows: (1) day 1, before administration, and 10, 20, 30, 45, 60, 90, 120, 150, 180, 240, 300, and 360 minutes after administration; (2) before administration in the morning on days 9, 11, 13, 23, 25, 27, and 29; and (3) day 30, before administration and 10, 20, 30, 45, 60, 90, 120, 150, 180, 240, 300, and 360 minutes after administration, to collect 3 mL of venous blood. Blood samples were centrifuged for 10 min at a speed of 3,000 r/min at 4 °C, and the plasma supernatant was transferred to a cryogenic tube for testing. The plasma concentration of CGA was measured with the LC-MS-MS method, and the main PK parameters, AUC