Evaluation of different transport media and growth conditions for periodontal biofilm cultivation
In order to select the most suitable transport media for biofilm samples from deep periodontal pockets, subgingival biofilms from five volunteers were collected in three different transport media (RTF, VMGIII and VMGIII Agar) and stored at room temperature for 0h, 24h and 48h (We also performed principal component analysis (PCA) comparing the microbial composition of 24 periodontal samples collected in VMGIII transport media (initial inoculum) to derived biofilms grown in the impedance system collected after 8h (