Species annotation
Paired-end sequencing of the GM samples obtained from both groups of patients was performed using the Illumina NovaSeq sequencing platform. Tiling and quality control were performed on the resulting data to obtain clean tags, which were then filtered for chimerism to yield effective tags that could be used for subsequent analysis. Clustering of OTUs was performed on the effective tags with 97% concordance (identity), followed by species annotation of the OTUs. A total of 2646 OTUs were identified, of which 755 were common to both groups, 1172 were unique to the treatment group and 719 were unique to the control group (species annotation of the GM in the two groups of OP patients. FRA represents the control group and YGD represents the treatment group. 