Receptor binding assay result
OVARY
UP165 (Lot. No. FP041019-01) was tested in duplicate at 10 concentrations for its MT1 and MT2 binding affinity. UP165 showed a fourfold increase in MT2 receptor binding affinity. UP165 had a dose-responsive curve with an ICPercent inhibition of ligand binding to MT1. Human recombinant CHO-K1 cells expressing the human melatonin MT1 receptor were incubated with UP165 at 10 concentrations and run in duplicate. The percent inhibition for the test compound was calculated compared to a 1% DMSO vehicle control. CHO, Chinese Hamster Ovary; DMSO, dimethylsulfoxide; MT1, melatonin receptor 1.Percent inhibition of ligand binding to MT1. Human recombinant CHO-K1 cells expressing the human melatonin MT2 receptor were incubated with UP165 at 10 concentrations and run in duplicate. The percent inhibition for the test compound was calculated compared to a 1% DMSO vehicle control. MT2, melatonin receptor 2.