ELISA method for detecting the expression of Bcl‐2 and Bax
An appropriate amount of tissue was collected for later use after removal of blood, and weighed and transferred into a glass homogenizer. The tissue was rinsed with 5–10 mL of precooled PBS (0.01 M, pH = 7.4) to eliminate any residual blood and then ground thoroughly. The prepared homogenate was centrifuged at 5000