2.8. Statistical Analysis
ADVERSE EVENTS
Data were analyzed using a set of two two-factor mixed-design analyses of variance (ANOVA). In the first analysis, the within-subject factor (repeated measures) represented the supplementation effect (before and after) on the performance variables during MAE and biochemical parameters at rest, while the between-subject factor represented the group effect (supplemented or control). In the second analysis, the within-subject factor (repeated measures) represented the effort effect of MAE (baseline, immediately post MAE, and 6 h and 24 h post MAE) and the between-subject factor represented the group effect (supplemented or control). Significant interaction between factors was subsequently analyzed using Tukey’s post-hoc test. The reliability of assumptions of this statistical test was checked using the Shapiro–Wilk test for normality, and Levene’s test for the homogeneity of variance. Further, the effect size was estimated by eta-squared statistics (ƞStatistical analysis was performed using Statistica 13 software (TIBCO Software Inc., Palo Alto, CA, USA).All participants recruited for the study completed the study with no adverse events reported. The results of MAE before and after supplementation with the lyophilized black chokeberry extract are presented in The results of the analysis of serum levels of inflammatory markers at rest before and after the supplementation are presented in Statistical analysis (ANOVA) revealed a significant effect of interaction (supplementation × group) on the resting values of 8-OHdG, IL-6, IL-10, and TAC (Before the supplementation, statistical analysis (ANOVA) of inflammatory marker levels revealed a significant effect of MAE on 8-OHdG (After the supplementation, statistical analysis (ANOVA) revealed a significant effect of interaction (effort × group) on changes in the IL-6 and IL-10 levels, and TAC induced by MAE (Post hoc analysis revealed that IL-6 levels immediately after (31.3%, Further, as before the supplementation, a significant effort effect of MAE on 8-OHdG and myoglobin levels was noted, regardless of the group. Finally, TAC significantly decreased immediately after MAE, regardless of the group effect, and the levels before exercise were higher in the supplemented group than those in the control group, as was also noted for the values at rest after the supplementation period.