Lentiviral vector integration site analysis
Lentiviral vector integration sites were identified in DNA extracted from the MPs (transduced T cells) and from PBMC samples collected between 6 months and 15 months after infusion in patients exhibiting high levels of long-term persistence (>1% of transduced PBMCs based on Psi qPCR). The identification and quantification of each integration site was performed using next-generation sequencing using the SonicAbundance method