Post hoc digital pathology and artificial intelligence analyses of treatment-induced changes in steatosis, hepatocyte ballooning, and liver fibrosis
NASH, qSteatosis, steatosis
LIVER FIBROSIS, STEATOSIS
Unstained, formalin-fixed sections from paired liver biopsies (baseline and EOT) were examined using second harmonic generation/two-photon excitation fluorescence (SHG/TPEF) microscopy with computer-assisted analyses. The liver sections were de-paraffinized, and tissue scanning was performed on Genesis200 (a fully automated, stain-free multiphoton fluorescence imaging microscope) and analyzed using artificial intelligence (AI)-based algorithms (HistoIndex Pte. Ltd) to quantitatively assess qFibrosis, qSteatosis, and qBallooning, as described.To compare steatosis and liver fibrosis changes from baseline and after 48 weeks of treatment, as assessed by NASH CRN scoring and by AI digital quantitation (qSteatosis and qFibrosis), patients in the 4 treatment arms were categorized as Progressor, No Change, or Regressor (