Sample collection and anthropometric measurements
systolic blood pressure
STERILE, BRAIN
At baseline and the conclusion of each intervention period, participants visited the Brain, Performance and Nutrition Research Centre (BPNRC, Northumbria University, Newcastle, UK) in an overnight fasted state. Anthropometric measurements of body weight, body mass index (BMI), hip and waist circumference, systolic blood pressure (SBP), diastolic blood pressure (DBP) and body fat percentage by bioimpedance scale (Tanita BC-418) were taken. Venous blood samples were collected in serum SST and fluoride oxalate tubes for analysis of blood lipids and glucose, and EDTA tubes for the analysis of lipoprotein particle sub fractions (Fisher Scientific). In addition, at each visit, a morning spot urine sample was collected and stored on ice during the study visit before the sample was aliquoted into sterile 1.5-ml tubes and stored at − 80 °C until further analysis.