Coadministration of probiotics modulated some fecal metabolites and serum BAs.
Untargeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics analysis was performed to analyze targeted fecal metabolites. Data were analyzed by an unsupervised coordinate method, principal-component analysis (PCA); symbols representing the quality control (QC) samples were tightly clustered (Changes in the fecal metabolome. (A) Principal-component analysis (PCA) score plot of the probiotic (Pro) and placebo (Pla) groups before (0M) and after (3M) the 3-month intervention. “QC” represents quality control samples. Samples from different subgroups are shown in different colors. (B) Volcano plot showing differential metabolites between the probiotic group and the placebo group at 3 months. Significantly increased metabolites (UP [upregulated]) (fold change [FC] of >2 and At month 3, the signal intensities of some metabolites were significantly (5-hydroxytryptophan, cortisol, Serum bile acid compositions of the probiotic (Pro) and placebo (Pla) groups before (0M) and after (3M) the 3-month intervention. Bile acid quantification was performed by liquid chromatography-mass spectrometry (LC-MS). Download Differential fecal metabolites identified by liquid chromatography-mass spectrometry. Download 