Ethics approval and consent to participate
All procedures performed were conducted strictly in full accordance with the ethical principles and standards of the institutional and national research committee and with the 1964 World Medical Association Declaration of Helsinki and its later amendments (version 2008). All experimental protocols were independently reviewed and approved by the Institutional Ethical Committee, Faculty of Dentistry, Alexandria University (IRB NO 00010556- IORG 0008839). This study was registered by ClinicalTrials.gov NCT05749640: 24/5/2022. Informed written consent was obtained from all individual participants included in the study.