2.3. Comparison of Small Extracellular Vesicle Isolation Methods
Small EV protein, RNA, and particle concentration and size, as well as the particle to protein ratio and particle to RNA ratio, were compared between ultracentrifugation with three different starting volumes and Exoquick. Further microvesicles and plasma were analyzed as well.Overall, a small EV isolation with Exoquick performed superiorly in contrast to ultracentrifugation. Small EV concentration in sEVs-EQ was significantly increased in comparison with sEVs-UC 0.25 mL (median [IQR]: 5.93 × 10Isolation with Exoquick resulted in more particles and an equal amount of RNA compared to ultracentrifugation of 0.25 mL plasma. Even though protein amount was increased, we observed CD63, CD 81 and CD9 expression only in sEVs-EQ, and albumin expression was present in sEVs-UC, but not in sEVs-EQ (