Bioanalysis
BLIND
Voriconazole were determined by liquid chromatography tandem mass spectrometry (LC–MS / MS) by Shanghai Fangda Biotechnology Co., Ltd. The equipment used in the determination process was acquity ultra-high performance liquid chromatography (Nexera UHPLC LC-30A, Shimadzu, Japan) and mass spectrometer (SCIEX API 4000, Applied Biosystems, Canada). Under the multi reaction monitoring and positive ionization mode, the samples were pretreated with acetonitrile by protein precipitation method, and then 5 μL pretreated samples were injected into LC–MS/MS system. Analyst 1.6.3 software (Applied Biosystems, Foster City, California, USA) was used for data collection and analysis. The sample analyst always keeps blind to the random group during the test.The method is verified fully by selectivity, accuracy, precision, calibration curve and stability. The drug concentration was linear within the range of 0.010 ~ 10.000 μg/mL. The lower limit of quantification was 0.010 μg/mL. Calibration curves were linear with the Pearson correlation coefficients ranged from 0.9950 to 0.9969. There was no significant interference in selectivity and stability.