Enzyme-linked immunosorbent assay (ELISA) for the protein expression of ILC2-related cytokines
The levels of cytokines (IL-5/IL- 13) in serum and nasal lavage were detected using the enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. The detection sensitivities of the assays were as follows: IL-5, 7.8 pg/mL; IL- 13, 125 pg/mL.