Bulk 
LYSIS
Total RNA was isolated by homogenizing 10–20 mg of tissue in Qiazol Lysis Reagent (Qiagen, cat# 79306). The RNA was separated and precipitated using chloroform and isopropanol. Extracted RNA was washed with ethanol and then suspended in nuclease‐free water. RNA concentration was determined using an EPOCH (Take3, BioTek) spectrophotometer. Libraries were prepared with Illumina Stranded Total RNA Library Prep NEB Ultra II directional RNA library prep with rRNA depletion and RNA was sequenced using Illumina NovaSeq S4 Reagent Kit v1.5 150 × 150 bp Sequencing (100 M read pairs). Differentially expressed genes were identified using a 5% false discovery rate with DESeq2 version 1.30.00 and the hciR package. Data can be found on the Gene Expression Omnibus (GSE224900). KEGG, and REACTOME pathways were identified using the fast gene set enrichment analysis in MSigDB using a 10% FDR. Real‐time quantitative PCR was carried out with a CFX Connect real‐time PCR cycler (Bio‐Rad) following the manufacturers protocol for SYBR Green custom‐designed primers. All customer primers utilized were ordered from Bio‐Rad. Cycle threshold values of target genes were normalized to GAPDH gene expression, and then fold change values were calculated (ΔΔCT) with the respective PRE bed rest values as reference.