Thromboxane B2
CLOT
For the purpose of serum TxB2 measurement, the serum clot activator tube was incubated at 37 °C for 60 min immediately after blood withdrawal. Afterwards, the samples were centrifuged at 4000 × g for 10 min and stored at − 80 °C until analysis. To be able to measure plasma TxB2, the EDTA tube was immediately centrifuged after blood withdrawal at 4000 × g for 10 min and, after addition of indomethacin, stored at − 80 °C until analysis. All samples were analysed in the same laboratory by the use of enzyme immunoassay according to the manufacturer’s instructions (Item #10,004,023, Cayman Chemical, USA). Samples were analysed in duplicate to account for inter-assay variation, and the mean values were used in further analyses.