2.2. MSC Recovery and Culture
SEPARATION
The MSCs had been isolated using an enzyme-free explant culture from a healthy donor warmblood horse (gelding, aged 6 years) euthanized for unrelated reasons. The cells were isolated and expanded without xenogeneic serum supplements. A StemMACS™ MSC Expansion Media Kit XF human (Miltenyi, Bergisch Gladbach, Germany) was used as the culture medium, and 1% penicillin–streptomycin and 0.1% gentamycin were added during the explant culture. TrypLE™ Express Enzyme (Gibco™, ThermoFisher Scientific, Dreieich, Germany) was used for passaging. After the first passaging, CD14+ cells were depleted via magnetic cell separation as detailed previously [