3.5.1. LEfSe analysis.
In general, the LEfSe analysis provides a phylogenetic tree diagram of clustered species, presenting the changes in the important microorganisms between the different treatment groups. Remarkably, 20 microbiota species were found to have a changed relative abundance before and after SAAT therapy in Group A (Aa vs Ac, Fig. The graphical representations indicate the changes in gut microorganisms by SAAT and sham SAAT. In the phylogenetic tree diagrams of species clustering, green nodes represent microorganisms that play essential roles in the green group. The red nodes represent microorganisms that play significant roles in the red group. Contrarily, the yellow nodes represent microorganisms that do not play essential roles in both groups. SAAT = summer acupoint application treatment.