4.1. Study Design and Subject
ALBINO
The study was a double-blind experimental posttest design with a control group, conducted at the Hasanuddin University Animal Laboratory, Makassar, Indonesia. Healthy male Swiss albino mice aged 6–9 weeks and weighing 20–30 g were maintained under a temperature of 28 °C ± 2 °C and humidity of 50% ± 10% for a minimum of 1 week. All mice were shaved on the back regularly during the research.The topical preparation was curcumin purchased from Sigma-Aldrich, Inc. (St Louis, MO, USA). It was diluted in acetone at concentrations of 0.1 μM, 1 μM, 10 μM, and 100 μM. The UVB lamp source was 10 FS-40-T12 fluorescent sun lamps with a spectrum of 280–340 nm and a peak emission of 314 nm. The UVB lights were calibrated with a FLUX radiometer.