Statistical analysis
NAFLD, cirrhosis, metabolic dysfunction
REGRESSION, OBESE, CIRRHOSIS
The study was designed as an exploratory study without prespecified outcomes reported here or a statistical analysis plan. The primary aim was comparisons in markers of metabolic dysfunction between healthy individuals, obese individuals with NAFLD and patients with cirrhosis. Comparisons between the 3 study groups were calculated using analysis of variance (One‐way ANOVA) with posthoc test (Šidák) for multiple comparisons. Total area under the curve (tAUC) was calculated with the trapezoid rule. For incremental AUC (iAUC), baseline values were subtracted and iAUC was calculated including only positive values above baseline. Net AUC (nAUC) was calculated as the area of peaks above baseline, subtracted with the area of peaks below that baseline. Peak concentrations were calculated as mean ± SEM of individual peaks in the different groups. Two‐way ANOVA with meal and group as categorical fixed effects and individual subjects as random effect was used to analyze tAUC, iAUC, nAUC, and peak concentrations. A posthoc test (Šidák) for multiple comparisons was performed for differences between groups. Shapiro–Wilk test and QQ‐plots were used to test the distribution of residuals. We used Pearson correlation and simple linear regression to correlate FGF21 concentrations to clinical and biochemical patient data. GraphPad Prism Version 9.1.1 and R statistical software were used for statistical analyses and graphical illustrations. Data are expressed as mean ± SEM if not stated otherwise. 