Plasma analyses
EMD
At each time point (Total ghrelin was measured using Human (Total) Ghrelin Kit (EMD Sigma-Aldrich). The kit has a sensitivity of 30 pg/mL in a 20 μL sample size, and samples were run in duplicates. During measurement of total ghrelin, samples outside the quantification range of the standard curve were further diluted with assay buffer in accordance with protocol and remeasured.The Millipore Human Metabolic Hormone Magnetic Bead Panel 96-Well Plate MILLIPLEX