3.3. Measurement of α-Dicarbonyls in Plasma
Fasting blood samples were collected into Heparin S-Monovette tubes (Sarstedt, Nümbrecht, Germany). Before analysis, all plasma samples stored at −80 °C were thawed and mixed thoroughly. The concentration of α-dicarbonyls in plasma samples was measured with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) according to the method proposed by Scheijen et al. [