2.2 Transcriptomic analysis of lymphoblastoid cell lines
We collected RNA sequencing data on LCLs (Coriell Cell Repository, Camden, NJ, USA) before and after exposure to busulfan. Briefly, we performed true-seq RNA-sequencing of mRNA extracted from six LCLs (GM7056, GM12239, GM12762, GM12057, GM12489, and GM12546) pre and post 48h of 100 μM busulfan treatment. The chosen concentration of busulfan represents 80% of cell viability in our set of LCLs with an average 50% inhibitory concentration of 415,1+/-203,3 μM. To avoid having non-genetic cell-to-cell variability in gene expression which could be introduced by the culturing condition, we fixed a limit on the maximum number of cell culture medium passages of 15 times.Library construction and sequencing were performed at the iGE3 Genomics Platform–CMU, Geneva, Switzerland, using the Illumina HiSeq 2000 library preparation kit. The differential expression analysis was performed with DESeq2 [