Fecal microbiota analysis by metagenomic sequencing
T2DM
Fecal samples from donor and T2DM patients were collected on the day of the medical examination and immediately frozen at −80°C. Fecal genomic DNA was extracted using the QIAamp Fast DNA Stool Mini Kit (Qiagen, CA, USA). DNA samples were stored at −20°C before use as templates for next-generation sequencing library preparation. Samples were fragmented to an average insert size of 400 bp and sequenced by Illumina Nova seq with PE 150 reagents. Reads were trimmed using KneadData with default parameters to filter the sequencing adapter, low-quality reads, and the human genome. The taxonomic composition was processed using kraken2 (