Mouse plasma multiplex analysis
Portal vein plasma levels of total glucagon-like peptide-1
(GLP-1), peptide YY (PYY), ghrelin, leptin and glucagon were measured by
multiplex assay kits based on chemiluminescence detection, following the
manufacturer’s instructions (Meso Scale Discovery [MSD], Rockville, MD,
USA). Analyses were performed using a QuickPlex SQ 120 instrument (MSD) and
DISCOVERY WORKBENCH 4.0 software (MSD).