Generation PPARα CRISPR knock-out cells
KNOCKOUT
The PPARα knock-out cells were created using a Cas9-based, CRISPR system. Two different sgRNA oligos from the human GeCKO v.2 Human CRISPR Knockout Pooled Library (Addgene; #1000000048), PPARa HGLibA_37838 and HGLibB_37787, were cloned into the lentiCRISPR v2 plasmid (Addgene; #52961). The sgRNA cloning was performed according to the human GeCKO v.2 system instructions as previously described (The lentiCRISPR v2 plasmid is a kind gift from Prof. N. Benvenisti, Stem Cell Unit at the Hebrew University, Jerusalem, Israel.