2.3.2. Targeted Assay of 2′-Fucosyllactose
2′-Fucosyllactose (2′-FL) was quantitated by LC-MS/MS. Human heparinized plasma was spiked with the internal standard, melezitose, and subjected to protein precipitation with methanol. After centrifugation, the organic supernatant was removed, evaporated, and reconstituted in Methanol/Water (75:25). An aliquot of the reconstituted extract was injected into Agilent 1290/AB Sciex QTrap 5500 LC-MS/MS system equipped with a BEH Amide normal phase UHPLC column. The peak area of the 