Flow cytometry analysis of AML PDX and cord blood transplants
AML
LYSIS, AML
For monitoring AML engraftment, 25–50 μl of PB were stained after red cell lysis (BD Pharm Lyse, BD Biosciences) with anti-human CD45-AF647 (H130) and anti-mouse CD45.1-PE (A20). For AML phenotyping, cell populations were purified from bone marrow (both femurs and tibiae) or SPL and after red blood cell lysis stained with anti-human CD45-FITC (H130), anti-mouse Cd45.1-PerCP/Cy5.5 (A20), anti-human CD34-PE (581), anti-human CD33-APC (WM53), anti-human CD38-APC/Cy7 (HIT2) and anti-human GPR56-PE/Cy7 (CG4). Flow cytometry analysis of lipid peroxidation was performed using C11-BODIPY 581/591 (Sapphire Bioscience) according to a previously published protocol