Quantification of parasites by qPCR
Parasite densities were quantified daily and retrospectively (cohort A) or prospectively (cohort B) on days 6 to 10 after immunization by real-time quantitative PCR (qPCR) and prospectively on days 6 to 21 after challenge (cohort A). qPCR was performed on the 18S ribosomal RNA genes as described previously [