Association of Exoria‐labelled pEVs with dermal fibroblasts
A series of cell‐based studies were performed to assess the biological activity of the LEAP‐isolated pEVs for wound healing. First, to confirm that the isolated pEVs retained the ability to be taken up by relevant cells, pEVs were labelled using the lipophilic dye Exoria, which has been used to monitor cell uptake in previous reports (Tertel et al., Confocal microscopy images demonstrate (a) pEV association with NHDFs, which increases over time of incubation, as opposed to (b) Exoria control, where no signal is detected. Red: Exoria‐labelled pEVs, blue: DAPI (nucleus). Scale bar: 10 μm.