4.8. Sample Analysis: Whole Exome Sequencing (WES)
tumors
TUMORS
In total, DNA from 34 tumors and 29 buffy coat samples from 29 patients were profiled by WES in 2 batches. The library preparation was performed using SureSelect Low Input v6 + UTRs with 150 ng of DNA. Exome capture was followed by massive parallel sequencing on Illumina HiSeq 2500 and 4000 instruments with 125 base pair reads. Upon data reception, reads were processed using Trimmomatic (v0.35) [