Data and sample collection
BLOOD, STERILE
Participants were enrolled at 5-8 weeks of gestation. Blood and stool samples were collected and followed up at 11-13 weeks of gestation and 24-28 weeks of gestation. During the follow-up period, filled in the questionnaire during the corresponding pregnancy, and left the participants’ blood samples and stool samples at two time points. All the 52 participants who were finally included in the study took blood samples and stool samples in both periods. All samples were collected in sterile tubes and stored at -80 °C until testing. The data of biochemical indexes such as glucose and lipid metabolism of pregnant women were obtained through the medical record system. After blood samples were collected, the immunological parameters IL-6 level was detected in the laboratory department. Fecal samples were collected for gut microbiota analysis.