RNA isolation from formalin-fixed paraffin-embedded samples
Cancer
CANCER
To obtain total RNA, pretreatment breast tissue samples were obtained from the archives of the Department of Pathology at Barretos Cancer Hospital. Formalin-fixed paraffin-embedded (FFPE) samples were subjected to total RNA isolation using the QIASymphony automated platform with the QIAsymphony RNA mini kit (Qiagen), following the manufacturer’s instructions. As a selection criterion for inclusion/exclusion, the samples were evaluated using NanoDrop 2000 (Thermo Fisher Scientific, USA) for quality analysis using the ratios 260/280 and 260/230 and Qubit (Invitrogen) for RNA quantification.