2.2.6. Measurements of Cytokines in Plasma Isolated from Peripheral Blood
The plasma obtained from the PBMC isolation step was used for cytokine measurement. The concentrations of IFNα2a, IFNβ, IFNγ, IL-10, IL-12/IL-23p40, IL-12p70, IL-15, IL-23, and MCP-1 were measured using the U-PLEX Biomarker Group 1 (human) Assays (MSD Maryland, Rahway, NJ, USA) in duplicate samples. The measurements were performed according to the manufacturer’s protocol, and the data were collected using MESO Quick Plex SQ 120 (MSD Maryland, Rahway, NJ, USA). The data processing and concentration calculations were conducted using DISCOVERY WORKBENCH 4.0 (MSD Maryland, Rahway, NJ, USA).