Statistical analysis
The sample size was calculated using previous literature [For statistical analysis, SPSS version 20.0 software (SPSS Inc., Chicago, IL) was used. The mean, standard deviation, or median are used to express continuous variables (interquartile range). The t-test was used for inter group comparison if the data were normal, instead that, the non-parametric test was used to compare groups. P-values less than 0.05 were considered statistically significant.