2.2.2. Biochemical Evaluation
INSULIN SENSITIVITY
After 12 h of fasting, a venous blood sample was obtained to measure alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-Chol), low-density lipoprotein cholesterol (LDL-Chol), and triglycerides (Tg) using available commercial kits. To calculate Matsuda ISI, we performed an oral glucose tolerance test using 1.75 g/kg of the body weight of anhydrous glucose (to a maximum of 75 g). Samples were taken at 0, 30, 60, 90, and 120 min, and glucose and insulin were determined. The Matsuda-ISI (Insulin Sensitivity Index) was calculated as follows: 10,000/√ (fasting glucose [mg/dL] × fasting insulin [mU/L]) × (mean glucose × mean insulin) [Tg, TC, and HDL-Chol were measured using standardized enzymatic methods, and LDL-Chol was calculated using Friedewald’s method.