Muscle metabolome
MPS
DER
The muscle metabolome was measured using biopsies at time point 0 min and 240 min after the intake of cocktail, both at 0 month and after 12 months of intervention. Muscle samples were extracted using a similar method as described by Alves et al. 2015 [Sample derivatization and injection of 1 µL derivatized aliquot were automated using a Dual-Rail MultiPurpose Sampler (MPS) (Gerstel, Mülheim an der Ruhr, Germany) as previously described [