IHC
breast cancer
BREAST CANCER
By means of the standard avidin-biotin complex method, the staining procedure was performed for IHC, with the extent of positively stained cells scored (0–10). In addition, the ZIC2-stained sections were divided into two groups (High and Low) based on scores of the staining between breast cancer tissues and paired normal adjacent tissues, with two pathologists evaluating all the specimens in a blinded manner.