Pyrimethamine exposure does not lead to a reduction of the inducible HIV-1 reservoir
To assess whether in vivo HIV-1 transcription induced by pyrimethamine or VPA alone or in combination affected the inducible viral reservoir, we used a tat/rev-induced limiting dilution assay (TILDA) to quantify the frequency of cells expressing multiply spliced (MS) HIV-1 RNA upon ex vivo cellular activation with phorbol 12-myristate 13-acetate (PMA)/ionomycin at baseline (day 0, 