Preparation for MASCT-I
tumor
TUMOR
MASCT-I cells were manufactured by HRYZ Biotech Co. in a Good Manufacturing Practice grade facility according to the manufacturing protocol as previously described with some modifications [For dendritic cell preparation, PBMCs were thawed and incubated in a culture flask (Corning) at 37 °C, 5% COTo prepare tumor antigen-specific T cells, the frozen PBMCs were thawed and co-cultured with antigen-loaded mature DCs (as described above) in the presence of IL-2 (1000 IU/mL), IL-7 (10 ng/mL), IL-15 (10 ng/mL), IL-21(30 ng/mL), and anti-PD-1 antibody (7.5 μg/mL) for 5 days, followed by T cell expansion with anti-CD3 antibody (50 ng/mL) for another 2 days. The culture medium was then replaced with fresh AIM-V medium supplemented with IL-2, IL-7, IL-15, IL21, and anti-PD-1 antibody, and cultured for another 1–2 weeks to obtain tumor antigen-specific T cells.The quality of DCs and T cells should meet the specification-releasing of MASCT-I before releasing for cell infusion.