FMT regulated the structure of gut microbiota
HEAT
Alpha diversity could reflect richness (ACE, Chao1, and Observed species), diversity (Shannon and Simpson), and evenness (J) of microbial communities within the sample. Results of 16S rRNA high-throughput sequencing analysis of gut microbiota before and after FMT. As shown in After standardization according to the operational taxonomic unit (OTU), the top 30 species with the largest number were selected, and then heat maps were created. The correlation method was selected by default for the clustering distance of species in the heat map. Each color block in the heatmap represented the abundance of a taxonomic level for a sample, and different colors indicated different proportions of species abundance. Linear discriminant analysis Effect Size (LEfSe) was used to compare multiple groups, and subgroup comparison was performed within the group comparison. Linear discriminant analysis (LDA) was used to reduce the data and evaluate the influence of significantly different species (LDA score) to find the species with significantly different abundance between groups. After FMT, the patients were rich in 