2.8. Determination of Homocysteine and Vitamin D Levels in Blood Serum
Tecan
The concentration of vitamin D metabolite 25 (OH) was determined by Diagnostyka SA with the automated chemiluminescence immunoassay method (CLIA) using the LIAISON XL device (DiaSorin, Vercelli, Italy). Serum homocysteine concentration was determined by ELISA (Bioassay Technology Laboratory, Shanghai, China) according to the manufacturer’s protocol. Absorbance was measured using a spectrophotometer (Sunrise, Tecan, Männedor, Switzerland) at 450 nm.