2.8. Supplementation Protocol
ADVERSE REACTIONS
In a randomized, double-blind, placebo-controlled fashion, participants supplemented at a dose of 10 mL/kg body mass for seven consecutive days with either standard spring water (as a placebo) or water with increased amounts of bicarbonate and other minerals ((BMW) Borjomi, IDS Borjormi, Tbilisi, Georgia). The dose of 10 mL/kg was decided upon through consultation with the sponsor as a dose that many recreational fitness enthusiasts may commonly consume each day. The mineral composition of the bicarbonate mineral water was as follows: bicarbonate (3700–4900 mg/L, chloride (230–480 mg/L, calcium (13–135 mg/L, magnesium (14–41 mg/L), sodium (940–1900 mg/L, potassium (11–42 mg/L), and other minerals (3.8–7.8 mg/L). Individuals assigned to complete the placebo condition were assigned to ingest bottled spring water with negligible bicarbonate. The composition of the spring water was also evaluated: bicarbonate (3.41–3.87 mg/L, chloride (0.33–0.52 mg/L, calcium (1.13–1.37 mg/L, magnesium (0.29–0.55 mg/L), sodium (0.96–1.49 mg/L, potassium (0.18–0.46 mg/L), and other minerals (0.41–0.96 mg/L). All bottles were identical in size and color with similar labeling. All supplements were stored in identical environmental conditions. Participants were instructed to ingest their doses at similar times of day. On testing days, participants ingested their required daily dose approximately 45–60 min prior to beginning their exercise tests. Participants completed a log to record their compliance with the supplementation protocol and any adverse reactions to their assigned supplement group. All randomization and blinding were completed by the manufacturer prior to shipment. To evaluate the effectiveness of the blinding protocol, participants reported which supplement condition they thought was administered to them following their final study visit.