Surgical procedures
teeth mesially, tooth
All interventions were performed after administration of an adequate local anesthesiaPreparation of L-PRF (For all groups an envelope flap was performed through sulcular incisions extending two teeth mesially and one tooth distally followed by mucoperiosteal flap reflection. Debridement of the periodontal lesion was then performed as well as planing of the exposed root surfaces. Following copious saline irrigation, reassessment of the bone defect morphology was performed and recorded. Being in a soft membranous form, the folded L-PRF harvest was easily grasped with tissue forceps and placed within the intrabony defect. The supplied PRF compactor was then used to tuck the L-PRF membrane within the defect up to the level of interproximal alveolar crests for L-PRF and L-PRF+CM groups. For L-PRF+CM, CMPrimary closure of the flap was performed with 5–0 Polypropylene sutures using internal vertical mattress design. A demonstration for the surgical protocol for every group is shown in Fig. Presentation of a study patient for every group (