2.5.2. Flow Cytometric Analysis of Circulating Extracellular Vesicles
Three-label flow cytometry analysis was assessed as previously described by Suades et al. [Acquisition was performed at 1 min per sample at a low flow rate. Forward scatter (FSC), side scatter (SSC), and fluorescence data were acquired using settings configured on the logarithmic scale. Gate limits were established following the criteria previously described [Data was analyzed with the BD FACSDiva™ Software (version 6.1.3). The concentration (number of cEVs per µL of plasma) was determined according to Nieuwland’s procedure [