2.4. Clinical Parameter Assays
ab181421, TNF-α, ab178013
Serum assay of clinical parameters was collected into labeled Eppendorf tubes at Ibn Al-Haytham Hospital, Clinical Laboratories Department, Jordan.The chemiluminescence immunoassay LIAISON 25OHD assay (DiaSorin, Saluggia, Italy) measured total serum 25OHD. The assay quantifies serum 25OHD and is cross-reactive with 25OHD2 and 25OHD3. Its lower limit was 4 ng/mL. An enzyme immunoassay kit measured serum leptin levels (leptin EIA-5302, DRG Diagnostics, Marburg, Germany). Test sensitivity was 0.1 ng/mL. An enzyme immunoassay kit tested serum PTH levels (PTH Intact EIA-3645, DRG Diagnostics, Marburg, Germany). The sensitivity was 1.57 pg/mL. The calcium-ARSENAZO kit (M11570i-15) and the phosphorus phosphomolybdate/Uv kit (M11508i-18, BioSystems, Barcelona, Spain) were used to measure the levels of calcium and phosphorus (PO4) in serum. Serum IL-6 concentration was measured using the Human ELISA KIT (ab178013, Abcam, Newark, NJ, USA). Using a Human ELISA Kit, serum IL-10 was measured (ab185986, Abcam). Human ELISA KIT assessed serum IL-1β (ab214025, Abcam). The Human ELISA KIT assay measured serum TNF-α (ab181421, Abcam).