2.4.4. Oxidative Stress and Inflammatory Markers
±, tumor necrosis, TNF-α
TUMOR NECROSIS, OXIDATIVE STRESS
Oxidative stress and inflammatory markers were analyzed by ELISA or colorimetric kits (Cayman Chemical Company, Ann Arbor, MI, USA) and read on a plate reader (Multiskan FC, Thermo Scientific, Beijing, China). All measurements were performed in plasma or serum samples according to the manufacturer’s instructions. Interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), interleukin 1 beta (IL-1β), total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS) and glutathione peroxidase activity (GPx) were analyzed at rest (pre-TTE). Changes (Δ: Post-Pre) in these markers after TTE were also determined (TACTTE, SODTTE, CATTTE, TBARSTTE, GPxTTE). All samples were analyzed in duplicate. Coefficient of variation of duplicates were: IL-6: 5.2 ± 5.0%, TNF-α: 21.5 ± 18.4%, IL-1β: 21.9 ± 26.8%, TAC: 9.8 ± 7.2%, SOD: 5.6 ± 7.9%, CAT: 4.4 ± 3.6%, TBARS: 3.1 ± 3.7%, and GPx: 4.4 ± 3.4%.