2.2. Experimental Protocol
SECONDARY
This was a double-blind, placebo-controlled, parallel design study. The randomization was performed by a researcher not involved in laboratory measurements using a block scheme stratified by age and systolic BP (SBP) with a computer program [The primary endpoint was the change in endothelial function, as assessed by brachial FMD. The secondary endpoints were reductions in PWV at rest and BP responses to CPT. Tertiary endpoints were changes in serum glucose, insulin, ARG, CIT, ORN, ADMA, arginase I, and oxidative markers.Participants completed a health-history questionnaire and were familiarized with the protocol. Height and weight were measured using a stadiometer and beam scale (free-standing portable height rod and weigh beam, Detecto, Webb City, MO, USA), and waist circumference was measured using a non-elastic tape. Body mass index was calculated as weight (kg) divided by height squared (m