Characterization of pEV proteome by mass spectrometry
shock
HEAT, SHOCK
To establish the proteome of pEVs isolated by LEAP, purified pEVs were digested and analysed by mass spectrometry. A total of 928 proteins were identified by this process, including expected EV biomarkers such as CD9, Annexins, and heat shock proteins. Extracellular vesicles generally display membrane proteins that mimic the parent cell (French et al., Mass spectrometric analysis revealed the abundance of proteins present in clinical pEVs based on whether they are involved in: (a) molecular function, (b) biological processes and (c) subcellular localisation.