Blood sample measurements
Pishgaman sanjesh-
After a 12-h over-night fasting, 20 ml of venous blood was taken from a brachial vein of eligible participants to evaluate serum levels of vitamin D, ALT, aspartate aminotransferase (AST), alkaline phosphatase (ALP), and Gamma glutamyl transferase (GGT). All blood samples were collected in EDTA anticoagulant tubes in the morning. Samples were then centrifuged at 5000 g for 15 min at 4 °C to separate the serum and its aliquots. Samples were stored immediately at − 80 °C.Serum 25(OH)D concentrations were measured by commercial ELISA kits (Pishgaman sanjesh- Iran), using an Awareness/Stat Fax 2100 analyzer [