Sample size calculation
Sample size calculation was performed using G*Power version 3.1.9.2. Prolactin level in GCF after treatment was the primary outcome. A pilot study was conducted on three patients in each group (included in the study groups afterwards). Mean values were 22, 18.8, 17.5, and 18 for the four groups, respectively. Standard deviation within each group was assumed to be 4. The effect size 