4.5. Profiling of Phytochemical Composition by UHPLC-MS
SEPARATION
The UHPLC (ExionLC AD, AB SCIEX, Framingham, MA, USA) separation was carried out using an EXIONLC System (Sciex). The mobile phase A was 0.1% formic acid in water and the mobile phase B was acetonitrile. The column temperature was set at 40 °C. The auto-sampler temperature was set at 4 °C and the injection volume was 2 μL. A Sciex QT 6500 + (Sciex Technologies, AB SCIEX, Framingham, MA, USA), was applied for assay development. Typical ion source parameters were: IonSpray Voltage: +5500/−4500 V, Curtain Gas: 35 psi, Temperature: 400 °C, Ion Source Gas 1:60 psi, Ion Source Gas 2:60 psi, DP: ±100 V [