Visit 13 (study week 12)
BLOOD
Procedures performed in visit 7 were repeated.The primary functional measure was the distance walked in 6 min or 6-min walk (6 MW) distance at baseline and final visit in all participants in both whey and EAA groups. In addition, body composition was determined at baseline and final visit of participants in both groups by dual-energy X-ray absorptiometry. Blood plasma samples were processed and analyzed commercially, and statistical data analysis performed to examine the differential protein expression in the plasma of subjects taking EAAs vs whey. Blood samples were collected in lavender potassium EDTA tubes, and then spun down to separate out the plasma. Plasma was then aliquoted into flip-top PCR tubes and shipped frozen on dry ice, Washington University (WashU, St. Louis, MO) for SOMALogic analysis.