Correlative Tissue and Blood Biospecimens
PD, tumor
TUMOR
Metastatic tumor biospecimens were collected during preregistration, end of cycle 1, and PD. Two tissue cores were formalin fixed and paraffin embedded while 1 core was immediately frozen at −70 °F. Formalin-fixed and paraffin-embedded tissues were sectioned at 5 microns and immunohistochemistry (IHC) staining performed using the ERα antibody, prediluted, clone SP1 (Ventana) and total AURKA antibody, diluted 1:100, clone JLM28 (Leica Microsystems).