Statistical analyses
The SPSS software version 23.0 was used for statistical analysis. The continuous variables were shown as mean ± standard deviation, and independent-samples t test was used for comparison between two groups. Repeated measures data were analyzed using ANOVA followed by post hoc analysis with Bonferroni adjustment for multiple comparisons. Count data were expressed as frequencies (%) and compared by Pearson's Chi-square test. The power calculator software PASS used for statistical power calculations. A value of 