Soluble factors
BLOOD
Blood samples (10 mL) were drawn in 10 mL red-top tubes to evaluate the serum levels of soluble CEACAM-5, CEACAM-6 and MICA. Blood samples were drawn prior to the first infusion dose on C1D1, 72 h post EOI, 14 days post EOI and prior to C3D1. After drawing, blood was processed with the same procedure described for cytokines analysis. The day of the assay, an aliquot of serum for each time point was defrosted and used at 1:3 dilution. Serum levels of soluble factors were detected through ELISA using the following kits and following manufacturer’s instructions: soluble CEACAM-5: Human Carcinoembryonic Antigen ELISA Kit (Abcam, Cambridge, MA, USA); soluble CEACAM-6: Human CD66c / CEACAM6 (Sandwich ELISA) ELISA Kit (LSBio, Seattle, WA, USA); soluble MICA: MICA Human ELISA Kit (Thermo Fisher Scientific, Waltham, MA, USA).