Preparation of the BMC
acid-citrate-dextrose
CREST
Prior to bone marrow collection, 1000–1500 IU/mL heparin was used to flush and rinse all the instruments. 1.5 ml acid-citrate-dextrose solution was then added to a 10 ml syringe as an anticoagulant. Subsequently, 8.5 ml bone marrow was slowly aspirated. A total of 30–40 ml bone marrow was extracted by iliac crest aspiration. The bone marrow aspirate was centrifuged using A-BMC (Aeon™) to autologous BMC. After the HTO operation, 4–6 ml bone marrow aspiration concentrate was injected into the knee joint.