IRAK4 detection by flow methods in PBMCs
IRAK4 degradation was evaluated in PBMCs using flow methods. Frozen PBMCs were thawed into RPMI with 10% FBS, and 90 μl of the solution was plated per well. KT-474 was prepared at a 10 μM starting dose, followed by fivefold dilution and a 10-point dose curve and added at a final DMSO concentration of 0.1%. Cells and compound were incubated at 37 °C and 5% COPercent IRAK4 signal was identified in B cells (CD3