2.8. Plasma Analysis
BLOOD
Blood collected in EDTA-coated tubes was immediately stored on ice, centrifuged, and plasma was stored at −80 °C until analyses Blood collected in serum tubes was stored at room temperature for at least 30 min to allow coagulation, followed by centrifugation and storage at −80 °C until analyses. Glucose (Horiba, Montpellier, France), FFA (WAKO, Neuss, Germany), and triglycerides (Sigma, St. Louis, MI, USA) levels were measured colorimetrically using a Cobas Pentra C400 analyzer (Horiba, Montpellier, France). All samples from one participant were analyzed within the same run.