Study design and setting
HEART
The participants performed two different trials in random order and separated by at least 5 days for the following purposes: (1) anthropometric and body composition measurements and completed in random order, no exercise (control, resting group) or (2) SIT performed on a cycle ergometer (Monark 894 E, Monark, Varberg, Sweden). Each trial began at 07:00 a.m. after an overnight fast (10–12 h). They were instructed to avoid caffeine, alcohol, strenuous, and exhaustive physical activity for 2 days before the exercise tests. For the no exercise (control, resting group) and the SIT trial, participants rested in a supine position for 30 min and then either ingested an oral carbohydrate liquid meal (Meal; partial hydrolysate of starch; 75 g glucose in a volume of 250 ml, this is the standard solution used for the oral glucose tolerance test; Dextrosol uva HYCEL; Grupo JAFS). The participants then returned to a supine position just after completing the oral glucose load (75 g) for 1 h. After experimental condition (control or SIT trial), participants remained in rest position 60,120, and 180 min after the ingestion of a 75‐g oral glucose challenge, (Figure Overview of experimental protocol (a), heart rate, and lactate response from eight‐repeated 30‐s “all‐out” intervals (b). Study design (a). Face mask icons represents indirect calorimetry (metabolic cart) assessments. The soda cup icon represents the 75‐g oral glucose dose. The drop icons represent the capillary blood sample assessments. Other assessments were performed only on day 1 (not shown). The study protocol timeline is expressed as hours. BIA, Bioelectrical impedance analysis assessment. Muscle strength (grip strength), anthropometry, and BIA assessments were performed only on day 1. Heart rate (HR ●) and blood lactate (□) concentration relationships to eight‐repeated 30‐s “all‐out” response (b). Data represent mean (standard error). RMR, resting metabolic rate.