Statistical analysis
Sample size was estimated a priori using G*Power 3.1.9.7 (Dusseldorf, Germany). The following options were selected in the software: test family = f tests; statistical test = ANOVA repeated measures, within-between interaction; type of power analysis = compute required sample size—given α, power, and effect size. Input parameters into the software were as follows: effect size f = 0.4; α error probability = 0.01; power = 0.95; number of groups and measurements = 2; correlation among measures = 0.5; nonsphericity correction = 1.0. The required sample size was 16 subjects per group (total sample size = 32). Data distribution was checked using the Shapiro–Wilk test. Homogeneity of variance within the groups was tested via Levene’s test Analysis of variance (ANOVA) with repeated measures or one-way ANOVA was used to analyze the data obtained. In case of significant changes (