Migration assay
A scratch assay was performed to determine if LEAP‐purified pEVs influenced cell migration. Briefly, 12,000 NHDFs per well were seeded on a 96‐well plate. When a confluent monolayer was formed, a uniform scratch was made in each well by AccuWound 96 tool (ACEA Biosciences, USA). The wells were washed to remove cell debris, and pEV treatments (15 μg/mL) were added to the appropriate well. After 24 h of treatment, the wells were stained with Hoechst nuclear stain, imaged, and the number of migrated cells was quantified using ImageJ.