Concordance of microbial results
PJIs, PJI
JOINT INFECTION
The pathogens based on their frequency of isolation by culture and detection by mNGS are shown in Correspondence between culture growth and the pathogen detected by mNGS.*The values are given as the detected frequency of a corresponding pathogen in all specimens. mNGS, Metagenomic next-generation sequencing.Including Including Including Including Including Including Including Including Including Pathogens from partial concordant, discordant and negative-culture PJIs in the subset of patients with previous antibiotics therapy.PJI, Periprosthetic joint infection; mNGS, Metagenomic next-generation sequencing.Similarly, in cases without prior antimicrobial therapy, 16 cases (29.1%) tested positive for mNGS but negative for culture, 1 case (1.8%) tested negative for mNGS but positive for culture, 6 cases (10.9%) showed identical negative results, and 32 cases (58.2%) showed both positive culture and mNGS results. Of the 32 double-positive cases, there was complete concordance between culture and mNGS results in 24 cases (75.0%). Eight cases showed partial concordance (5 of 32 [15.6%]) or discordance (3 of 32 [9.4%]) of the microbial findings (Pathogens from partial concordant, discordant and negative-culture PJIs in the subset of patients without previous antibiotics therapy.PJI, Periprosthetic joint infection; mNGS, Metagenomic next-generation sequencing.The complete concordance of positive mNGS and positive culture results was similar in both groups (44 of 55 [80.0%] with previous antimicrobial therapy vs. 24 of 32 [75.0%] without previous antimicrobial therapy, p=0.586). The overall concordance analysis of all cultures from all sources and mNGS results is shown in Distribution of mNGS and culture results in all cases (N=131) and concordance analysis between positive mNGS and positive culture cases (double positive, N=87).