Statistical analysis
Primary endpoint will be the measure of response to PD-1 checkpoint blockade (overall response, CR, and PR) in patients with IFN-γ–related gene expression signature. The expected response rate in patients with IFN-γ–related gene expression signature is 60%, the expected two-sided alpha error is 0.05 and beta error is 0.2, the required number of patients is calculated to be 54 based on the exact binomial test (Casagrande et Pike) and considering the patients excluded from the analysis due to censoring. DEG analysis between responders and non-responders will be performed using DESeq2 package. IFN-γ–related gene expression signature and other genes include in the panel will be considered differentially expressed if log 2-fold change is at least ± 2 and 