Exosome validation by transmission electron microscopy (TEM)
TEM
The morphology of the exosome was identified using TEM. Briefly, a suspension of freshly isolated exosomes was dropped onto a formvar carbon-coated copper electron microscopy grid (Plano, Wetzlar Germany). The grid was then negatively stained for 1 min with 2% uranyl acetate solution, washed with PBS, and dried at room temperature. Finally, images were acquired with an EM 900 transmission electron microscope (Zeiss, Germany) at a voltage of 80-90kv.