2.5.1. Platelet Aggregation
platelet aggregation
TRANSMISSION
The study of platelet function was performed by analyzing platelet aggregation induced by different agonists: arachidonic acid (1 mM), collagen (2 and 5 µM), and ADP (5 and 20 µM) by the light transmission technique LTA (“Light Transmission Aggregometry”) in platelet-rich plasma prepared within an interval of 30 min from blood withdrawal at the different time-points of the study. The preparation contained 250,000 platelets/µL of platelet-poor plasma from each individual that was used as a 100% light transmission control. The recording of platelet aggregation was performed for a period of 5 min and was used for the calculation of the maximum aggregation [