In vitro drug susceptibility assays
parasitemia
LYSED, PARASITEMIA
Circulating breakthrough parasites were re-cultured and susceptibility to mefloquine in serial dilutions was determined at 0.83% parasitemia and 3% hematocrit. After incubation for 72 h at 37 °C, parasites were lysed and relative parasitemia was determined by measuring DNA bound SYBR green fluorescent signal.