Limited features are significantly altered in treatment or constipation-associated phenotypes in this study
bloating, pain
REGRESSION
Via a discovery-driven MAS, we aimed to determine if the abundance of specific taxonomies or pathways was altered at baseline versus the endpoint as a function of treatment, bloating, pain, and WBMs. Adjusting for age and baseline WBMs, we used logistic regression to evaluate the association between baseline abundance of each microbiome taxonomic group across phylogenies as well as pathways identified in our sequencing data. We identified substantial heterogeneity in our cohort in these microbial feature abundances, so we only analyzed pathways and taxa that occurred in ten or more samples, yielding a total of 185 taxa and 2213 pathways. Within these, we identified one statistically significant relationship after correcting for multiple hypothesis testing: a decreased abundance of We additionally computed beta diversity between individuals in the treatment group at all phylogenetic levels (using MetaPhlAn3 output) and did not observe clear stratification by treatment status (Supplementary Fig. 