Statistical Analysis
According to the Fleming single-stage design, assuming that the expected percentage of patients with ΔKi67 ≥25% would be at least 40% for each treatment group and the minimum acceptable rate 10%, a total of 12 patients per treatment group provided 90% power with a two-sided α of 10%. Differences among the three treatment groups, pretreatment and posttreatment were evaluated using the Kruskal–Wallis test. If the result of the Kruskal–Wallis was found to be significant, All tests were two sided, and significance was set at the 5% level of significance. 