Validation by real ST data with double-blind histopathology annotations
tumor
TUMOR
We next evaluated the performance of SpaCET with real tumor ST data. The generation of an ST profile typically provides a hematoxylin and eosin (H&E)-stained image from the same tissue slide. According to the H&E morphology, pathologists labeled the local tissue density and regions of tumor, stroma, lymphocyte, and macrophage without knowing any deconvolution results. We applied SpaCET to decompose eight tumor ST datasets