4.3. Measurement of Biochemical Parameters
BLOOD
After fasting for 8 to 10 h, blood samples were obtained with vacutainer equipment in tubes (Beckton-Dickinson, NJ, USA) with an EDTA anticoagulant to perform a complete blood count and quantify the percentage of HbA1c. Likewise, samples were taken without an anticoagulant for biochemical tests (glucose, total cholesterol, HDL-cholesterol, triglycerides, uric acid, urea). Blood count was performed with a whole blood sample in a hematology analyzer (Spincell, Spinreact, Girona, Spain). The measurement of the biochemical parameters and the CRP was carried out with serum samples using an autoanalyzer (Selectra Junior, Siemens, Munich, Germany) through colorimetric and turbidimetry techniques, respectively. HbA1c was measured in EDTA anticoagulated blood samples via turbidimetry in the Selectra Junior autoanalyzer.