Measurement of anti-sporozoite antibody titers
malaria
MALARIA
Nunc MaxiSorp™ 96-wells plates (ThermoFisher) were coated overnight at 4 °C with 100 μl, equivalent to the lysate of 40,000 ELISA analyses were performed using Auditable Data Analysis and Management System for ELISA (ADAMSEL FPL v1.1).A pool of 100 sera from adults living in an area in Tanzania where malaria is highly endemic served as positive control serum. The standard curve was plotted on a logarithmic scale and fitted to a power trend line (R2 > 0.99). Optical density (OD) measurements for each test sample were converted to arbitrary units (AU) relative to the control serum (control serum was set at 100) and normalized for the Xmid of each plate.