Blood collection and biochemical analysis
TG
After an overnight fast, 25 mL of blood was collected in BD Vaccuette® tubes (Greiner Bio-One GmbH, Kremsmünster, Austria) with EDTA or without anticoagulant to obtain plasma and serum, respectively, that were separated by centrifugation and stored at − 80 °C until analysis.Biochemical analysis in serum was performed following reference procedures recommended by the Spanish Society of Clinical Biochemistry and Molecular Pathology. Lipid metabolism biomarkers were determined using a Roche Cobas Integra 400 plus analyser (Roche Diagnostics, Mannheim, Germany). TC, TG and high-density lipoprotein cholesterol (HDL-C) were determined, along with apolipoproteins A1 (Apo A1) and B (Apo B). LDL and VLDL (very low-density lipoprotein) were calculated according to Friedewald formula, and Apo B/Apo A1, LDL/HDL and TC/HDL ratios were calculated [