Luminex
Gingival Crevicular Fluid (GCF) was collected using PerioPaper (Oraflow) from six sites with pocket probing depth ≥ 4 mm (3 maxillary sites and 3 mandibular sites), at baseline, 1 day, 3 days, and 7 days after treatment. The GCF strips were eluted and analysed by performing a multiple bead immunoassay technique (Luminex, Biotechne; R&D Systems). Eight GCF markers were assessed in total: IL-6, Insulin, VEGF, Angiogenin, C-Reactive Protein/CRP, IL-10, Leptin/OB, and Vitamin D.