Statistical analysis
T2D
In our trial tissue sodium, muscle water and muscle fat content in the lower leg were specially measured with a 3.0 T clinical MR system (Magnetom Skyra, Siemens Healthineers, Erlangen, Germany) using a custom-made transit/receive sodium RF birdcage knee coil (32.6 MHz, Stark Contrast, Erlangen, Germany). Reliability as well as accuracy have been shown previously [In brief, patients were in supine position with the thickest part of their left or right calf muscle at the centre of the sodium RF birdcage knee coil with calibration tubes placed in a phantom holder underneath the calf and included in the field of view. Partial volume effects and short relaxation times of sodium ions result in a more than threefold underestimation of measured sodium concentrations and in order to reflect this, the signal intensities are reported in arbitrary units (AU). Proton and sodium images were then acquired (Fig. Sample of Interrater variability calculations showed according to Bland Altman [Statistical analysis was performed using SPSS Statistics 28. The sample size was calculated for the primary endpoint using data acquired during a previous trial, the SD of change in skin sodium content was 4.17 AU in patients with T2D [All analyses of this trial were performed in patients presenting at least a 