Proximate analysis of KDP and WHEY
SEPARATION, ASH, TAG
Proximate analysis was carried out according to the 1990 Association of Official Analytical Chemists methods. Amino acids were analysed in triplicate with a standard hydrochloric acid hydrolysis followed by RP HPLC separation using AccQ Tag derivatization. Cysteine/methionine were analysed using performic acid oxidation (AOAC 994.12). The moisture, ash, fat, and protein contents (g/100 g) in the samples were determined as follows; the protein content was determined using Kjeldahl (AOAC Method 991.20), moisture content was determined by drying at 106 °C for 24 h (ISO R-1442) and ashing was done at 550 °C for 24 h (ISO R-936). The fat content was measured according to Soxhlet method (ISO R 1443) using a Foss Tecator AB Soxtec 2050. Carbohydrates was determined by subtracting the sum of moisture, ash, fat and protein content from 100. The caloric value was calculated as described by Karp et al. [