NA-UC-MSCs preparation
Bifarma, infectious disease, HIV, Hepatitis B
INFECTIOUS DISEASE, SYPHILIS, HEPATITIS C
Human umbilical cords were obtained from the cesarean section in the maternity ward of a hospital in Jakarta from a qualified donor that passed donor screening and testing of infectious disease (HIV, Hepatitis B, Hepatitis C, Syphilis, CMV IgM) and karyotyping. The human umbilical cord was subsequently processed to yield MSCs in Regenic Laboratory PT. Bifarma Adiluhung, Jakarta, Indonesia, with GMP standard. The MSCs were isolated with Alpha MEM (+ GlutaMAX) + Human Serum + Growth Factors and Vitamin + Antibiotic Antimicotic 1% and then passaged to passage 7 with Alpha MEM (+ GlutaMAX) + Human Platelet Lysate. To positively identify MSCs, the markers CD105, CD73, CD90 (> 95%) and CD45, CD34 CD14, CD19 and HLA-DR (< 2%) were used; and also Adipogenic, Osteogenic, Chondrogenic Differentiation were tested.