Study design
GROUP B
A randomized controlled trial was conducted. The primary outcome was the fluorescence intensity (FI) of the common bile duct and liver at three timepoints: before surgical dissection of the cystohepatic triangle, before clipping the cystic duct, and before closure or before CBDE. The second outcome was the cholangiography effect, which was analyzed by the bile duct-to-liver ratio (BLR). Patients were randomly allocated for preoperative injection with one of the four doses of ICG (Group A: 1 µg; Group B: 10 µg; Group C: 25 µg; and Group D: 100 µg). This study was approved by the Research Ethics Committee of the Guangzhou First People’s Hospital (approval NO: B202203101) and complied with the requirements of the Declaration of Helsinki. This study was registered in the Chinese Clinical Trial Registry (ChiCTR No: ChiCTR2200064726).