References
CeD, ZED1227-TG2
Immunofluorescence staining of transglutaminase 2 (using mab CUB 7402, green fluorescence) and gliadin peptides (mab A161, red fluorescence) in a duodenal biopsy from a CeD patient. The majority of the total TG2 is localized near the basement membrane and in the lamina propria. The luminal surface of the villous epithelium is labeled with equally strong intensity (panel (Localization of ZED1227 by mab A083 immunofluorescence (red) in a duodenal biopsy from a ZED1227-treated patient at low magnification (panel (Comparison of quantified ZED1227-TG2 fluorescence intensities in the villous epithelium and lamina propria in the patient groups who received placebo, 10 mg, 50 mg, or 100 mg of ZED1227. The last oral drug dose was given 24 h before endoscopy and biopsy sampling (panel (Confocal microscopy imaging of total TG2 (mab CUB 7402, in green) and ZED1227 (mab A083, in red) by double immunofluorescence staining (panel (Panel (TG2 activity is demonstrated with 5-biotin pentylamine labeling in a cultured human epithelial organoid. The TG2 inhibitor ZED1227 (0.02 mg/mL) caused a reduction of 5-BP staining intensity in the apical epithelial surface compared to the DMSO control (arrows). Panel (