2.5. Psychometric Questionnaires and Dietary Intake Assessment
depression, eating behavior, mood disturbance
Validated questionnaires were used to elicit data related to participants’ physical activity, mood, sleep habits, and eating behavior at both timepoints. These questionnaires included the International Physical Activity Questionnaire (IPAQ) short form [The MFQ assesses mood disturbance over the past two weeks, with a maximum score of 66 and higher scores indicating greater severity. Scores ≥ 27 are considered indicative of depression [Female participants were additionally asked about the status and typical duration of their menstrual cycle as well as their current use of hormonal contraceptive medication. All questionnaires were administered via paper with the exception of the IPAQ, which was administered using an electronic spreadsheet (Excel, Microsoft, Redmond, WA, USA) on a laboratory computer. Lastly, participants reported all food and drink (excluding water) consumed during the full calendar day prior to the visit using the electronic Automated Self-Administered 24-Hour Dietary Assessment Tool (ASA24, National Institutes of Health, Bethesda, MD, USA). All questionnaires and reports were self-administered, though participants were provided verbal instructions necessary for completing each questionnaire and were encouraged to ask questions and request assistance from the attending investigator as needed. When administering the IPAQ, participants were instructed to report their current habitual levels of physical activity rather than their activity over the past week.