Tissue micro array
TMA, Lynch Syndrome, colon biopsies block
BREAST CARCINOMA, LYNCH SYNDROME, OVARY CARCINOMA
A total of 36 Formalin-fixed Paraffin-embedded (FFPE) colon biopsies from Lynch Syndrome participants (18 pre-treatment and 18 post-treatment tissue samples) were collected. Three unstained slides (4 µm thickness) sections were obtained from each sample to perform hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining. A pathologist (L.S.) evaluated H&E slides to determine the quality of the sample, selection of areas for tissue microarray (TMA) construction, and histomorphometry analysis. Two biopsy blocks were excluded due to artifacts and insufficient tissue for analysis. One single TMA block was created using up to two tissue cores (1 mm diameter) from each colon biopsy specimen for further Image Mass Cytometry (IMC) staining. Normal and neoplastic control tissues (appendix, lymph node, tonsil, spleen, placenta, breast carcinoma, and ovary carcinoma) for IMC staining were included in the TMA of colon biopsies block to evaluate the staining quality from each immune marker.