Post-hoc correlative analyses
In a post-hoc analysis prompted by clinical observations during the trial, cytokines in plasma were analyzed by the FCCC High Throughput Screening Facility utilizing the Human Chemokine Panel 40-plex (Bio-Plex Pro, Bio-Rad cat # 171AK99MR2) following manufacturer’s protocols and plates were read on a Bio-Plex 100/200 (Bio-Rad Laboratories, Hercules CA). Plasma underwent cryopreservation at −80 °C via standard operating procedures and was later used for batch cytokine analysis. The instrument was driven with Bio-Plex Manager (version 6.1.0727) software and data was analyzed and exported utilizing Bio-Plex Data Pro software (version 1.2.03).