Plasma biomarkers of mild cognitive impairment to AD progression
A1AT
ADHESION
The following plasma proteins were measured using multiplex bead assays (Luminex xMAP): acid glycoprotein (AGP), apolipoprotein C3 (ApoC3), pre-albumin, alpha-1 antitrypsin (A1AT), pigment epithelium-derived factor (PEDF), complement component 4 (CC4), intercellular adhesion molecules-1 (ICAM-1), regulated on activation, normal T cell expressed and secreted (RANTES), clusterin and cystatin c. Median fluorescent intensity (MFI) of the xMAP assays were measured using the xPONENT 3.1 (Luminex Corporation) The MFI were exported into Sigma Plot (Systat Software; Version 12.5) for estimation of protein concentrations using a 5-parameter logistic fit.