Oral glucose tolerance test and biochemical measurements
BLOOD, INFLAMMATION
For the OGTT, overnight fasted subjects ingested a standardised glucose solution (75 g). Blood was drawn from an intravenous catheter at baseline and 15, 30, 45, 60, 90 and 120 min after ingestion. Both blood serum and plasma were aliquoted and stored at −80 °C. From these aliquots we measured glucose and C-peptide, which was performed by the Endocrinology department of the Amsterdam UMC. In addition, blood samples collected at baseline and follow-up were used to measure fasted glucose, insulin, HbA1c and the clinical safety parameters for renal/liver function and inflammation, all measured by the Central Diagnostics Laboratory of the Amsterdam UMC. Results were reported in the electronic medical record software from EPIC (versions August 2019-November 2020).