Additional tissue immunological analyses
Nivo+CT, tumor
TUMOR
The distribution of baseline PD-L1 expression by immunohistochemistry (IHC) in tumor cells according to MPR and treatment arm is depicted in Extended Data Fig. To assess the impact of adding Ipi to a backbone of Nivo+CT on the tumor microenvironment, we used multiplex immunofluorescence (mIF) staining and flow cytometry of tissues pre-therapy and post-therapy. mIF analyses revealed significantly higher densities of CD3