Quantification of serum cytokines
inflammation
INFLAMMATION
Serum levels of Th1 inflammation (IFN-γ, TNFa, IP-10, Th-17), Th2 inflammation (IL-4, IL-5, IL-13), and monocyte activation (MCP-1, and IL-6) were measured using Milliplex human cytokine/Chemokine/Growth factor magnetic bead panel A (MilliporeSigma, St. Louis, MO) and the Luminex 200 platform (Luminex Corp, Austin, TX). The samples were processed according to the manufacturer’s instructions and the cytokine concentrations were quantitated by the Luminex xPONENT v3.1 and MILLIPLEX Analyst v5.