SAMPLE SIZE AND ELIGIBILITY CRITERIA
teeth showing enamel defects, caries lesions, orthodontic
Ten orthodontic patients scheduled for extraction of the four first premolars (n=40) as part of their treatment plan were included. To be included, patients were required to be in good health, not be taking any medications, and not receiving any other medical treatment. Patients with teeth showing enamel defects or caries lesions (white spots or cavitation) observed under clinical analysis were excluded from the present study. The sample size calculation was based on the reduction in the enamel mineral loss in the 1% TiFAll clinical examinations and procedures were done by a non-single-blind calibrated operator at the orthodontic clinic of 