Assessment of ultra-structure surface morphology alterations
tooth
EROSION
Scanning electron microscopy (SEM) JSM-IT200 InTouchScope™ Scanning Electron Microscope (JSM-IT200 Series, JEOL; Tokyo, Japan) was used for this purpose. Two teeth from each group were sectioned into buccal and lingual specimens using a double-sided fine grit water-cooled diamond disc yielding 4 specimens. One specimen of each tooth was randomly selected for baseline assessment of ultra-structure surface morphology alterations, while the other one was used for post erosion assessment. All specimens were dehydrated at the time of evaluation by passed through series of 50%, 70%, and 95% ethyl alcohol for 10 min each and then in absolute alcohol for two changes of 1-h period each. This was followed by drying in vacuum desiccator for 1 h [