2.1.3. Fluorescence Activated Cell Sorter (FACS) Analysis
FRANKLIN
After 24 h of cocultivation, PBMCs were stained with FITC-labeled anti-human CD304 (clone No. U21-1283), PE-Cy7-labeled anti-human CD123 (clone No. 7G3), APC-labeled anti-human CD86 (clone No. 2331), and PE-labeled anti-human HLA-DR (clone No. G46-6) (BD, Franklin Lakes, NJ, USA) following treatment with Horizon Fixable Viability Stain 780 (BD, Franklin Lakes, NJ, USA) and Human BD Fc Block (BD, Franklin Lakes, NJ, USA) treatment. The cells were then washed twice with Stain Buffer and fixed with Cytofix Buffer (BD, Franklin Lakes, NJ, USA) for FACS analysis. Flow cytometry was used for analysis of surface markers of pDCs by selecting pDCs defined as CD123