4.4. Laboratory Assessments
All blood samples were collected between 8 and 10 a.m. after an overnight fast and stored at −80 °C until analysis. Commercial enzyme-linked immunosorbent assays (ELISA) were used to measure serum concentrations (pg/mL) of IL-6, IL-10, IL-1RA, CCL-2 (all BioVendor, Brno, Czech Republic), and HMGB-1 (ng/mL) (IBL International GmbH, Hamburg, Germany) according to manufacturer’s instructions. IL-6 to IL-10 was also expressed as ratio, reflecting cytokine balance. The omega-3 plasma index was measured in phospholipid fractions by gas chromatography as described elsewhere [