Molecular response of dermal fibroblasts to LEAP‐purified pEV treatment
The effect of pEV on vital cellular pathways was assessed to determine the mechanisms by which pEVs may induce the observed range of cellular responses in treated cells. After treatment with pEVs, AlphaLISA assays were performed to detect ERK and Akt phosphorylation in NHDFs over the course of 2 h (Figure Measurement of (a) pAkt/Akt and (b) pERK/ERK ratios over time when untreated (black) or treated with pEVs (red). Comparison of (c) Akt and pAkt levels, and (d) ERK and pERK levels in cells after 5 min incubation with clinical pEVs (red) as opposed to untreated cells (white). 