Allelic status determination
tumor, heterozygous loss, number loss
EVENTS, TUMOR, DELETION
Allelic status for enrollment genes was determined by SNiPDx, WGS or local NGS (where available). Enrollment genes were considered to have biallelic LOF if one of the following criteria was met: (1) homozygous deletion; (2) compound heterozygous mutation; (3) mutation and LOH; or (4) mutation and non-overlapping loss. Enrollment genes were considered to have monoallelic loss if the following criteria were met: (1) mutation without LOH or (2) heterozygous loss, considered to have no loss if no mutation or copy number loss was detected. CHIP was determined in cases where the enrollment alteration was detected in PBMCs but determined not to be germline. Subclonal alterations were those where the enrollment alteration was detectable at lower-than-expected VAF, upon adjustment for tumor purity, ploidy and local copy number, or present only in some tumor biopsies. If central results were not available and local testing could detect any of the above events leading to biallelic loss, the gene was considered to have biallelic loss. Allelic status calls were reviewed by an external board-certified molecular pathologist.