Ethics approval and consent to participate
All experiments for all patients were performed in accordance with relevant guidelines and regulations. This preliminary clinical trial was registered on the Iranian Registry of Clinical trial (IRCT20200120046197N1). The study received ethical approval from the Ethics Committee of Shiraz University of Medical Sciences (IR.SUMS.DENTAL.REC.1398.139). a written Informed consent was obtained from all guardians of participants after clearly explaining the aims and probable risks and benefits of the study.