Curcumin gel preparation and application
eating hard, PD
The gel preparation was carried out in the Pharmacology Department, Faculty of Pharmacy, Mansoura University. The 2% curcumin gel was prepared by the method of simple dispersion (Table 
Formula to prepare 2%curcumin gel(HPMC = hydroxy propyl methyl cellulose; q.s. =quantity sufficient)0.2 mg of curcumin (Sigma, St. Lo, USA) was transmitted into an HPMC solution where it was further homogenized. After that, this drug solution was transmitted to a solution of carbopol where it was also homogenized. Then triethanolamine was further added in a quantity sufficient (q.s.) to make neutralization of the ph. The added distilled water made q.s. to 100 ml. finally, the gel was kept at ambient temperature. The prepared curcumin gel was verified and evaluated for microbial growth and activity using procedures including an incubation period of 24 h on an agar plate while associated microbial growth was observed. If no microbial growth and activity were noted, the final curcumin gel preparation was then kept in glass containers for storage in the refrigerator (humidity ranging from 80–85%and 2–10 °C temperature) until the time of use to avoid its subsequent damage [Following SRP procedures, only selected periodontal pockets (pockets diagnosed with stage II grade A, PD of 3–5 mm, and CALof 3-4 mm) [This procedure continued until the pocket was filled. Care was taken to apply the gel without traumatizing or damaging the periodontal tissues. After insertion of the curcumin gel, the region was secured with a cotton pack, and the patients were advised not to eat or drink for at least two hours following gel administration. They were also advised not to brush the treated areas or floss /use interproximal cleaning devices for at least twelve hours postoperatively and to avoid eating hard and abrasive foods that could traumatize the gingiva. The treatment was followed-up by weekly recalls after gel application for reinforcement of oral hygiene measurements and subsequent gel reapplication. The curcumin gel was applied weekly for four weeks after completing SRP.