NanoString nCounter® system assays
Breast Cancer
BREAST CANCER
Gene expression profiling was performed using the nCounter Breast Cancer 360 panel (NanoString Technologies, Seattle, WA, USA), according to the manufacturer’s protocol. Briefly, 100 ng of FFPE total RNA sample was hybridized with probes for 21 h at 65 °C; subsequently, the complexes were processed using NanoString PrepStation. Purified target-probe complexes were eluted and immobilized on the nCounter cartridge, which was transferred onto the nCounter Digital Analyzer for image capture (555 FOVs) and data acquisition.