2.9. Data Analysis and Statistics
DARLING
The data analysis was conducted using GraphPad version 9.4.0. The normal distribution of the pharmacokinetic data was tested using the D’Agostino–Pearson (omnibus K2) and Anderson–Darling methods. The area under the curve (AUC) from the first to the last sample of the respective intervention phase (AUCThe individual profiles of spermidine and spermine in plasma can be found in Sample 11 of the verum arm was compared with sample 11 of the placebo arm (8 h after first administration), and sample 25 of the verum arm was compared with sample 25 of the placebo arm (the last samples of each intervention phase). The respective samples from the two intervention phases (t = 0 h) were also compared to test the setup and baseline determinations. In addition, a Wilcoxon matched-pairs signed-rank test was performed to detect significant differences between the two intervention arms. All metabolites were plotted together in a forest plot to provide an overview of the changes in sample 11 of the verum arm versus sample 11 of the placebo arm, as well as in sample 25 of the verum arm versus sample 25 of the placebo arm (