Target engagement assessment
colon cancer
DNA DAMAGE, COLON CANCER
Paired biopsies from 2 patients were available to assess target engagement before and after treatment with TAK-164. Since the payload of TAK-164 is an alkylating agent, we utilized the DNA damage marker, γH2AX, as a surrogate marker of target engagement. Analysis of γH2AX staining from a patient with colon cancer receiving 0.19 mg/kg demonstrated target engagement with limited γH2AX staining present at baseline, and clear induction of γH2AX staining present following TAK-164 treatment (Fig. Biopsy imaging for γH2AX staining before baseline (