Data acquisition
Images were acquired with the Hyperion Imaging System (Fluidigm, Standard BioTools) according to the manufacturer’s instructions. After choosing the region of interest (ROI) in the section, the ROI was ablated with a UV laser at 200 Hz. Data were exported as .mcd files and visualized using Fluidigm MCD viewer 1.0.560.6. The minimum and maximum thresholds were adapted for each marker and for each tissue for optimal visualization. Gamma was set to 1.