4.6. DNA and RNA Extraction from Frozen Tissue Samples
tumor
STERILE, TUMOR, COLD
All procedures were conducted in an RNA-free environment. The OCT blocks were positioned on cold Petri dishes (placed on dry ice) with the tissue surface facing up. A sterile scalpel blade was used to remove the tumor tissue from the OCT block. If macrodissection was recommended by the pathologist (for tumor enrichment), only the portion delineated by the pathologist on the reference H&E slide was carved out and used for the extraction of genomic material. The frozen tissue was gently separated from the OCT and immediately placed in a pre-chilled Precellys