Tissue harvesting and processing in rabbits
dehydration, overdosage
DEHYDRATION
The rabbits were sacrificed by overdosage with isoflurane 8 weeks after IMRCs injection with anesthesia. After sacrificing, the organs and whole meniscus were placed in 4% ice-cold PFA. Images of the whole meniscus were taken on the same day of harvesting and further completed the dehydration, paraffin embedding, and sectioning of the tissues.