4.4. Limitation
Our research results were observed only at the macroscopic level, focusing on the actual changes in various cognitive functions of E-athletes. The findings confirmed that acute aerobic exercise contributes to the improvement of certain cognitive functions in E-athletes, providing a basis for further research. However, 1 limitation of our study is that we did not further explain the cellular and molecular biological mechanisms at the micro level. Exercise can significantly increase the levels of biologically active molecules associated with improvements in cognitive function, including insulin-like growth factor 1, growth hormone, vascular endothelial growth factor, and brain-derived neurotrophic factor. Subsequent research could delve into the changes in the content of these relevant biologically active molecules, further elucidating their mechanisms at the cellular and molecular levels. Additionally, near-infrared spectroscopy could be employed to further observe the brain imaging results of E-athletes, thereby investigating the activating effects of acute aerobic exercise on different brain regions of E-athletes.