Biochemical measurement
STERILE, CAVITY
Salivary samples of all the patients were collected at baseline and four weeks after the intervention to assess IL-8 and IL-1β levels. All the salivary samples were collected between 9 a.m. and 12 p.m. The patients were asked to refrain from brushing 12 h before sampling and refrain from eating, drinking, and chewing gum two hours before the sampling. To investigate the inflammatory interleukin levels, the unstimulated saliva of the patients was collected before any action. The patients were asked to avoid swallowing their saliva for five minutes, and then the accumulated saliva in the oral cavity floor was passively drooled into sterile disposable test tubes [