2.4. Blood Sampling and Analysis
BLOOD
After a 12 h overnight fast, samples (5 mL) were collected from the cubital vein using standard techniques. Blood samples were collected at baseline and 48 h after the last training session. Following completion of blood collection, samples were centrifuged at 3000 rpm for 20 min, and the serum was stored at −70 °C for future analysis of adiponectin, CTRP3, CTRP5, CTRP9, TNF-α, and CRP (ZellBio GmbH, Lonsee, Germany).