PCR analysis
recrudescence, parasitemia
RECURRENCE, PARASITEMIA, REINFECTION
DNA extraction was performed from DBS using QIAGEN kits (QIAGEN Inc., Hilden, Germany), according to the manufacturer’s instructions. The analyses were performed at the Parasitology Unit of the Institut Pasteur de Madagascar. For participants with recurrent parasitemia after day 7, paired polymerase chain reaction (PCR) blots (from day 0 and the day of parasitemia recurrence) were analyzed for parasite merozoite surface proteins (MSP-1 and MSP-2) and glutamate rich protein (GLURP) to distinguish between reinfection and recrudescence, as described elsewhere [