5. Limitations
DISEASES
This study presents some limitations. First, the sample size was small and the intervention period was short. Further studies should be conducted involving large samples and over long periods of time. Second, relevant indicators were not tested for the 4 week intervention outcomes. Moreover, some indicators, such as blood glucose and fasting insulin levels, were not tested. A greater understanding of time-restricted eating patterns is required, including the optimal duration and frequency of fasting, long-term compliance, and their effect on reducing various diseases. Furthermore, in view of the heterogenous findings reported to date, the effects of time-restricted eating in different healthy populations needs to be clarified in future studies.