4.3.4. Investigation of Oxidative Stress Parameters
The blood collected for biochemical analysis (in a tube containing coagulation activator) was centrifuged for 10 min at 4 °C and 2500 rpm. The resulting serum was pipetted into Eppendorf-type tubes and stored in a low-temperature freezer at −80 °C until further analysis. The assessment of oxidative damage parameters (concentrations of 8-OHdG, 8-iso-PGF2a, 4-HNE, and 3-NT) was performed using an immunoenzymatically method (ELISA test).