EV miRNA and proteome analysis.
LYSED
The miRNA content within EDC EVs was profiled using multiplex fluorescent oligonucleotide-based miRNA detection (Human v3, NanoString), as previously described (EDC EVs were lysed [8 M urea, 100 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 5% glycerol, and 0.5% n-dodecyl-β-Flow cytometry was performed on EVs that were individually labeled for CD9 (312106, BioLegend), CD63 (353004, BioLegend), or CD81 (349506, BioLegend) using a CytoFLEX S Beckman Coulter flow cytometer (