Serum cytokines
BLOOD
IL-12, IFNγ and CXCL12 were measured in patient serum. Blood was drawn once pretreatment (between days −30 and −1) and then again on days +3, +7, +14, +21 and +28 (±3 days) to isolate serum and collect PBMCs. ELISA was performed following manufacturer’s protocol (BD Biosciences). For measuring CXCL10, serum samples were run after 10-fold and 100-fold dilution. Undiluted serum was used for IL-12 and IFNγ measurement. Log range dilution of purified cytokines were used for generating standard curve. Absorbance was measured at 450 nm from 96-well ELISA plates. The raw data was organized using MS Excel and analyzed on GraphPad Prism version 9. The data was interpolated using a standard curve with a second order polynomial (quadratic) equation and plotted with a 95% confidence interval (CI). The correlation coefficient (R