2.5. Laboratory Procedures
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HEART
All tests took place under controlled environmental conditions (temperature: 19.6 ± 0.3 °C; barometric pressure: 1005.6 ± 1.2 mBar; and relative humidity: 48.4 ± 2.2%). Upon arrival, participants rested for 10 min in a seated position before assessment of blood pressure (Omron 750CP, Kyoto, Japan), body mass (electronic scale, Seca, Hamburg, Germany), and height (Seca stadiometer, Hamburg, Germany). At rest (baseline) and throughout exercise, 20 µL capilliarized fingertip blood samples were collected for the assessment of blood lactate and glucose (Biosen C Line EKF-diagnostic analyzer, Cardiff, UK). Heart rate (HR) data were recorded in 5 s intervals using a short-range telemetric monitor (Polar 810s, Polar T34 strap, Kempele, Finland). For the initial familiarization trial, body composition was also recorded using bioelectrical impedance for the indirect assessment of body fat percentage, fat-free mass, and fat mass (Tanita SC-330ST, Amsterdam, The Netherlands). Breath-by-breath pulmonary gas variables (volume of O