Tumor Biopsy Analyses
tumor, Tumor, breast cancer
TUMOR, TUMOR, BREAST CANCER
mIF was used to analyze changes in the tumor microenvironment (TME) induced by the combined immunotherapy. Samples were available from all 6 patients (five baseline, six surgery; five paired; Correlative analysis of tumor specimens. Representative baseline biopsy (Tumor samples were assessed using RNA-seq to further investigate the TME. Samples were available from all 6 patients (three baseline, six surgery, and three paired). Immune cell types in the resected samples compared with baseline biopsies, assessed by RNA-seq data deconvolution, showed distinct increases in gene expression associated with CD8RNA-seq and DNA sequencing analyses of biopsies. WES of tumor specimens was used to investigate the genetic drivers and potential tumor clonal dynamics with respect to therapy. Known genetic drivers of breast cancer were detected in biopsies of 5 patients, including 