Faecal glycosidic linkage levels and WLZ responses
The same faecal samples used for the DNA- and RNA-level analyses were processed for UHPLC–QqQ-MS-based quantification of 49 glycosidic linkages. This analysis focused on samples collected at the 0- and 3-month timepoints from MDCF-2-treated individuals in the upper and lower quartile of WLZ response. These linkages were measured after their liberation by in vitro hydrolysis of faecal glycans (Supplementary Table 