ALDRICH
PBMCs from patient samples at the time of discontinuation were thawed and incubated in RPMI 1640 medium supplemented with 10% heat-inactivated FBS (GIBCO, New Zealand). 2 mM L-glutamine and 100 U/mL penicillin/streptomycin (complete RPMI, all from Gibco, Carlsbad, CA, USA) plus 200U/ml DNase (Sigma Aldrich, St. Louis, Missouri, US) for 1 hour at 37°C in a humid atmosphere containing 5% CO3×10