Vaccinia detection and qualitative assessment of treatment effect in tumor
MPM, tumor, tumors
TUMOR, POSITIVE, INFILTRATE, MALIGNANT PLEURAL MESOTHELIOMA, TUMORS
Resected post-treatment samples from 14 patients were stained by immunohistochemistry with an antibody against Olvi-vec (A27L). Positive cytoplasmic expression was observed in 7 of 14 specimens (representative images shown in Visualization of Olvi-vec within tumor cells and the immune cell infiltrate in malignant pleural mesothelioma (MPM) tumors following Olvi-vec treatment. Resected samples from 13 patients were stained by multiplex immunofluorescence with a panel of antibodies against mesothelin (MSLN), CD3, CD4, CD8, and FoxP3. 4 patients had matched pre- and post-treatment specimens, and 9 patients had post-treatment specimens only. The density of MSLN+ tumor cells was qualitatively observed to decrease and the density of CD3+ immune cells was qualitatively observed to increase from pre-treatment to post-treatment specimens (representative images shown in 