RNA and DNA extraction
MP
RNA and DNA were extracted from tissue samples stored in RNAlater for RNA-seq, WES or Sanger sequencing. Tissue homogenization was performed in TRIzol (15596026; Life Technologies) using a FastPrep-24 5G Sample Preparation System (MP Biomedicals) as per protocol. Genomic DNA was extracted from approximately 25–30 mg of APA or adjacent normal adrenal tissue using a QIAamp DNA Mini Kit (51304; Qiagen) according to the manufacturer’s instructions. If AAG was not available to be used as a control, genomic DNA was extracted from blood using the salt extraction method. Total RNA was extracted from approximately 50–100 mg of AAG or APA tissue using the TRIzol method (15596026; Life Technologies) followed by on-column DNase I treatment (Invitorgen) and purification using Invitrogen’s PureLink RNA Mini Kit (12183018A).