Body composition
CREST
During the medical visits, body weight and height were measure barefoot and clothes off. BMI was calculated based on measured height and weight. Waist circumference was measured midway between the iliac crest and the lowest border of the rib cage. Body composition was assessed by bioelectrical impedance using a 4-polar single frequency device (RJL Systems, Model 101A; Detroit, MI, USA). The fat and lean body mass were predicted using the software Bodygram