Scanning electron microscopy and quantitation
WED
WED
The samples were collected in glutaraldehyde fixation buffer, dehydrated with graded ethanol, and treated with hexamethyldisilazane (HMDS; Ted Pella, Inc., Redding, CA) and left overnight for drying.For scanning electron microscopy (SEM) quantitation, the blinded samples were imaged following a standard protocol (WED significantly decreased biofilm severity in all burn patients. 