Intervention
MINST
STERILE, CAVITY, HIV PERIODONTITIS
Patients with stage III and IV periodontitis were allocated by the same examiner (RP) to 2 groups: The first group received MINST only (MINST group), and the second group received MINST and single-time subgingival application of collagen hydrogels with PACNs (MINST + PACNs group). Non–commercially available type I collagen hydrogels were manufactured for the research purposes of the current study.Prior to the clinical trial, patients received intraoral cavity preparation (emergency dental treatment, hopeless teeth extraction). Standardisation of the clinical trial was achieved by professional oral hygiene and individual oral hygiene instructions for each patient. During the 8-week term of the trial, the patients were informed not to use other chemical control materials.The clinical trial protocol included several appointments. At baseline, all patients received a periodontal examination, and saliva samples were collected. Samples of unstimulated saliva were collected with 3 sterile cotton rolls deposited for 5 minutes in the oral cavity and stored in sterile tubes at −80 °C. Both treatment groups underwent a full-mouth MINST procedure by a single periodontist (EA). The procedure was performed in a sterile field. Under local anesthesia, the participants underwent MINST with thin and delicate tips together with ultrasonic (Acteon Satelec Suprasson P5 Booster) and hand instruments (Gracey micro-curettes [Hu-Friedy] and mini-curettes [LM-Dental]) and magnification of 4.5×. During the MINST procedure, the periodontist was not informed about the patients’ group assignment (MINST or MINST + PACNs). After the procedure, patients’ group allocation was revealed to the periodontist (EA) by the examiner (RP) and, accordingly, each patient either received adjunctive treatment (the MINST + PACNs group received collagen hydrogel chips with PACNs placed subgingivally in periodontal pockets with PPD ≥4 mm) or had the procedure ended without adjunctive therapy (MINST group). Postoperative care instructions were given.Patients returned for a follow-up appointment 8 weeks after baseline. Periodontal reevaluation of encoded patients was performed by a single examiner (EA). Unstimulated saliva samples were collected in the same manner as mentioned earlier.