SAMPLE SIZE CALCULATION
A pilot study with 10 patients was conducted. The means and standard deviations of canine retraction (in mm) for the two, four, six and eight-week reactivation were 0.51±0.43, 0.64±0.25, 0.58±0.41, 0.90±0.41, respectively. The sample size using the means for six months of retraction was calculated using the repeated measure ANOVA in the G*Power v. 3.1.9.6 software, with a 95% CI and 0.8 power. A total sample size of 36 patients was estimated.