Coadministration of probiotics modulated the fecal microbiome composition but not the overall microbiota diversity.
This study analyzed the microbial metagenomes of 96 fecal samples (Microbial diversity and differentially abundant species-level genome bins (SGBs) between groups. (A) Shannon and Simpson diversity indices of the gut microbiota of the placebo (Pla) and probiotic (Pro) groups at month zero (0M) and after the 3-month intervention (3M). (B) Principal-component analysis (PCA) score plots for four subgroups. Symbols representing each subgroup are shown in different colors. (C) Significantly different SGBs between the probiotic and placebo groups at different time points. A Quality control information for the metagenomic data set. Download Analysis of similarity (ANOSIM) to evaluate differences in gut microbiota structures. Download Next, postintervention changes in the gut microbiota composition at a finer taxonomic level were analyzed. Responsive species-level genome bins (SGBs) (SGBs that were not significantly different between the two groups at day 0 but became significantly different between time points or different treatments only after the intervention) were identified. One responsive SGB that was differentially abundant between month 3 and the baseline was identified in each group: Significantly differential species-level genome bins (SGBs). Download 