Saliva sample collection
PAD, STERILE, -20
Saliva sample collection was accomplished using the swabbing method. It was performed by introducing a cotton pad into the mouth at the orifices of major salivary glands. The subjects were asked to chew the cotton pad to get soaked in the saliva. The saliva-soaked pad was removed and placed in a sterile test tube (sterile Eppendorf tube) and centrifuged at 3000 rounds per minute (RPM) for approximately 10 min and then kept frozen at -20°c till the time of analysis.