Assessment of CSCs' and bulk of the tumor chemotherapy response
tumor
TUMOR
Treatments with anti-cancer drugs and sensitivity tests were performed as described previously in.96-well plates are seeded in RPMI-1640 with 10% FBS, penicillin and streptomycin with a minimum of 20,000 individual tumor cells per regimen of bulk tumor cells or CSCs in 5 replicas and incubated at 37°C in a 5% COAfter the 1-h exposure, the treatment media containing the various chemotherapies were removed and replaced with fresh media. MTT assay was performed 24 h following chemotherapy treatment to assess cell survival as previously described.Inhibition of bulk tumor cells and CSCs survival was measured for each concentration (average counts in five replicates ±SE) of a given treatment (for a total of 15–18 different treatments per patient). Survival of tumor cells at each concentration was calculated as compared to control-2 and the overall percent of the bulk of tumor cells and CSCs killed was calculated for each treatment as the primary measures of potential therapy efficacy.