Genotype analysis
LYSIS
The genetic analysis for this study was performed by Muhdo health Ltd. (Martlesham Heath, Ipswich, England) and Eurofins Global Laboratory (Certification: ISO 17025:2005, ISO 17025:2017). Muhdo supplied the 1,000 SNP custom chip, capable of analysis with a 97–99.9% accuracy level (Phosphate buffered saline was used to prevent any premature cell rupture and cell recovery was accomplished using standard extraction procedures. Recovered cells and assay medium (Cell Lysis Solution Infinium, GoldenGate) were loaded into chip wells for Illumina multiplex sequencing. The custom chips (Illumina® Infimum HumanOmni BeadChip) with bound DNA sequences, containing target SNPs, were scanned using a Microarray Scanner (iScan, Illumina, San Diego, CA, USA). Fluorescence information was collated using Illumina’s standard software (Illumina’s GenomeStudio® software). The iScan Control Software automatically normalized the intensity of the fluorescence data to remove technical variation and generated genotype calls [