4.4. Dual-Color Immunofluorescence Staining
ZED1227-TG2
Dual-color immunofluorescence staining for ZED1227-TG2 and total TG2 was performed with mab A083 and mab CUB7402. Antigen unmasking and blocking of endogenous peroxidase activity were done as described in single-color staining. Primary antibodies and detection reagents were applied to the tissue sections as sequential incubations, employing three washes between the staining rounds. ZED1227-TG2 was detected with red fluorescence (CF594-tyramide) and total TG2 with green fluorescence (CF488-tyramide). Hematoxylin was used to counterstain.