Biofilm determination and removal
PLAQUE
Bioclear dual-color disclosing solution was applied to the thoroughly dried teeth using a micro brush applicator (Cotisen, China) and was left for 30 s to set followed by rinsing; as the newer plaque stains were pink and the plaque older than 24 h were stained purple. Then blasting with (aluminum oxide air–water slurry) aluminum oxide powder Jeep sandblasting device was performed until the entire biofilm was removed.