Generation of Feladilimab, and Surface Plasmon Resonance and Biolayer Interferometry
Feladilimab is a humanized variant of the murine mAb obtained from Institut Paoli-Calmettes, INSERM (Daniel Olive, Marseille, France). The murine antibody was generated by immunization of BALB/c mice with recombinant human ICOS-Fc (COS-7 cell-derived) and subsequent hybridoma production. Following clone screening, the selected clone was humanized and grafted onto an IgG4 hinge-stabilized isotype containing two amino acid substitutions (S228P/L235E), dubbed IgG4-PE (To evaluate feladilimab or recombinant human ICOS-L (His-tagged, Sino Biological) affinity for human ICOS, surface plasmon resonance analysis and biolayer interferometry analyses were performed (details in 