Hepatitis B virus genotyping results
hepatitis B, ’ blood
HEPATITIS B, VIRUS
Patients’ blood (487 samples in total) positive for HBsAg were obtained from the following regions of the Republic of Kazakhstan: Aktau, Atyrau, Oskemen, Turkestan, Taraz, Chimkent, Almaty, Astana, Kyzylorda, Pavlodar, Aktobe, Kostanay and Karaganda.In total, out of 487 samples, 341 samples were positive for PCR and genotyped (70% of the total number of submitted samples). Comparison and phylogenetic analysis of nucleotide sequences of PCR products of HBV isolates showed that they (n = 341) are represented by genotypes HBV-D (95.9%; n = 327), HBV-A (3.5%; n = 12) and HBV-C (0.6%; n = 2). At the same time, the identity of the nucleotide sequences of Kazakhstani isolates was: HBV-D (95–100%; n = 327); HBV-A (97.2–100%; n = 12) and HBV-C (99%; n = 2) (Table 
Data on genotyping of HBV isolates isolated in the territory of the Republic of Kazakhstan, by regionThe most common HBV genotype in Kazakhstan was the D genotype with a frequency of 95.9% of the total number of sequenced samples. This genotype met and prevailed in all the studied regions. Genotype A was found in 3.5% of cases, and was found in the following regions of the Republic of Kazakhstan: Turkestan, Taraz, Chimkent, Almaty, Kyzylorda and Pavlodar, mostly in the southern regions RK. Genotype C was found only in two cases, and these were samples from Almaty. Previously, the presence of this genotype in the territory of the Republic of Kazakhstan was not reported in the literature. In 21 patients with positive PCR of HBV DNA and HDV RNA, hepatitis B virus genotype D was detected.In current study the most common subgenotype was D1–61.5% (Fig. 
The prevalence of hepatitis B virus subgenotypes in the regions of Kazakhstan