The cutoff value and diagnostic power of positive FLD cfDNA
tumor, CRC
TUMOR, RECURRENCE
To assess the clinical utility of peritoneal cfDNA in diagnosis of PM, we identified the cutoff value of FLD MaxAF at best positivity threshold of 6.29% performed by ROC analyses with area under curve (AUC) of 0.951 in the training cohort (Fig. The accuracy of peritoneal cfDNA for detection of PM and prediction of recurrence. To verify the diagnostic power and accuracy of peritoneal cfDNA in detection of PM, we conducted NGS assays in the FLD of randomized CRC patients in a validation cohort of 11 patients. In the validation cohort, 5/6 (83%) patients with PM obtained positive FLD cfDNA, comparing to 0/5 in patients without PM (We investigated the associations between peritoneal cfDNA and clinicopathological features in PM. No significant associations were observed between FLD MaxAF and these clinicopathological parameters including age, gender, alcohol & tobacco, tumor location, CEA, CA199, CA125, PCI scores, T stage, N stage, and histological type. However, CA153 levels was positively correlated with FLD MaxAF (