Biopsy and histopathology
PROSTATE
All patients underwent systematic TRUS-guided biopsy after at least 4 weeks of the MRI examination, in which “10 + x” cores were obtained in a double sextant pattern, sampling the lateral and medial portions of the apex, mid, and base of each hemi-gland. After examination of mpMRI, patients with suspicious lesions underwent fusion biopsy by using an ESAOTE Mylab Twice color Doppler ultrasound device (with a 7.5-MHz transrectal end-fire probe), which equipped with a Real-time Virtual Sonography imaging fusion system.All fusion biopsies were performed by a urologist with 7 years of experience in prostate biopsy. Specimens (biopsy or radical prostatectomy) were assigned Gleason score (GS) by an expert genitourinary pathologist with more than 15 years of experience who was blinded to the MRI findings. All GS were assigned in concordance with the 2014 International Society of Urological Pathology consensus guideline [