Flow Cytometry and Cell Sorting.
PBMCs were thawed at 37C into complete RPMI media with 5% human AB serum (Gemini Bioproducts) in the presence of benzonase [20 mL/10mL], washed with PBS and resuspended in Fcr Block human TruStain FcX™ (1:20) and stained for viability (eFluor 506 viability dye, Invitrogen, 1:1,000). 3 × 10