neoTCR T cell generation
CD4 and CD8 T cells were positively enriched from healthy donor leukapheresis products by magnetic selection (Miltenyi) and activated for 48 h with CD3 and CD28 stimulation (TransACT, 1:17.5 by volume; Miltenyi) in T cell medium (TexMACS medium, Miltenyi, supplemented with 12.5 ng ml