Cell culture
BLOOD
We purified patient PBMCs from blood samples by density centrifugation over Ficoll-Paque Plus (GE Healthcare). We purified healthy donor PBMCs from buffy coats (New York Blood Center) and isolated T cells using a Pan-T cell isolation kit (Miltenyi Biotech). We activated T cells with CD3/CD28 beads (Thermo Fisher) with IL-7 (3,000 IU ml