Kinetic Profile of FCH absorption
Briefly, fasted volunteers received 12 g of FCH, and the absorption profile was monitored during a 240 min time period by measuring hydroxyproline blood levels. Analyses showed that following ingestion of FCH, circulating concentration of hydroxyproline continuously increases to reach a maximum of 117.7 µM at 140 min post ingestion (+ 87.5% compared to basal level), before returning to almost basal level by the end of the kinetics (Fig. 
Evolution of the concentration of circulating hydroxyproline in blood. Values are presented as mean ± SEM. The differences were considered statistically significant at p < 0.05 with * for p < 0.05; ** for p < 0.01; *** for p < 0.001; **** for p < 0.0001 and ns for p > 0.05 [Based on these results, both naïve and enriched serum with FCH metabolites for the ex vivo cultures were subsequently collected before ingestion (naïve serum) and at 140 min post-ingestion (enriched serum).