Calcium released to the citric acid–Comparison of both subgroups without (NP) and with salivary pellicle (P)
Irrespective of pellicle formation (P or NP), lowest values of calcium release were observed for positive control, without difference to GSE+NaF. When considering the factor presence of salivary pellicle, comparisons within each group showed significant differences for groups GSE, GSE+NaF, negative control and positive control. For groups GSE, GSE+NaF and negative control, the presence of pellicle (P) significantly reduced calcium release when compared to NP. As for positive control, higher calcium release was observed for P than for NP.