Urine collection
MSU, STERILE
Urine samples were provided by the hospital staff after collection in the morning. Midstream urine (MSU) samples or catheter specimens were collected using disposable, sterile containers, kept refrigerated for maximum 2 hours and split into 10 aliquots of 1 ml each: 4 were immediately analyzed by the hospital laboratory and with the MBS AST POCT, 3 were frozen at -80°C using sterile glycerol at a final 15% (vol/vol) concentration, while the other 3 were further divided in 100 μl aliquots, frozen at -80°C using sterile glycerol at a final 15% (vol/vol) concentration, and analyzed within 7 days, to assess the impact of sample freezing or in case of the need of further verification analysis.