Multiplex immunofluorescence (IF) analyses
tumour
TUMOUR
For multiplex IF analysis, the Opal chemistry, and multispectral microscopy Vectra/Polaris scanner system (Akoya Biosciences, Waltham, MA) were used; analysis was performed using the inForm software. A total of 21 cases were stained for Multiplex IF Panel 1, 2, 4, and 5 using similar methods to those previously describedAdditional multiplexed IF staining on a subset of available tissue samples for tumour and immune cell markers was also performed in FFPE tumour samples at screening, Day 14, and surgery using NeoGenomics MultiOmyx