Metabolic analysis of glucose, lactate, and glutamine
IST, SARS-CoV-2 infected
Metabolic analysis of SARS-CoV-2 infected culture medium in the BSL3 facility was done using Accutrend Plus multiparameter meter (Roche Diagnostics). Culture medium was collected every 48 hr and stored at –80 °C prior to analysis. Measurements were carried out using Accutrend Plus Glucose and BM-Lactate Test Strips according to the manufacturer’s instructions. Each measurement was done in 3 technical measurements for each sample, validated throughout the process using calibration medium. Glucose uptake, as well as lactate production, were calculated based on the difference between sample and control medium.Metabolic analysis of SARS-CoV-2 proteins expressing culture medium in the BSL2 facility was done using amperometric glucose, lactate, and glutamine sensor array (IST, Switzerland) as previously described (