2.4. Surgical Procedure
Following Phase I therapy, the patients were reevaluated to confirm the inclusion criteria. Extraoral and intraoral areas were disinfected with 5% povidone iodine and 0.2% chlorhexidine mouthwash, respectively. The interdental papilla was retained while a full-thickness mucoperiosteal flap was reflected after the surgical site had been anesthetized with 2% Xylocaine HCl and adrenaline (1:80,000). After flap reflection and exposure of the osseous defect, a thorough debridement was done followed by irrigation with Normal saline. Defect depth was measured from the bottom of the defect to the clinically visible cemento-enamel junction using William’s probe and graft placed after pre-suturing.Randomly selected intrabony defect sites were covered with the AmnioGuard [