2.6. Assessment of Blood Markers
MDD, TG
BLOOD, SENSITIVITY
Fasting blood samples were taken under standard conditions between 8 and 10 am from the right arm 12 h before the first exercise session and 72 h after the last session. Blood samples were transferred to EDTA-containing tubes, centrifuged for 10 min at 3000 rpm, and stored at −70 °C for later use for the following measurements:Plasma CTRP-9 was measured with an ELISA kit (Aviscera Bioscience, Santa Clara, CA, USA). Catalogue No: SK00081-02. Sensitivity: 1 ng/mL. Intra-coefficients of variation (CV) = 4%, inter-CV = 8%.CTRP-2 was measured using ELISA kits obtained from MyBioSource (San Diego, CA, USA) with a minimum detectable dose (MDD) of 0.039 ng/mL and detection range of 0.156–10 ng/mL (intra-assay CV: <8% and inter-assay < 10%).GDF-15 was measured using ELISA kits obtained from Thermo Scientific (Frederick, MD, USA) with a sensitivity of 2 pg/mL and detection range of 1.10–800 pg/mL (intra-assay CV < 10% and inter-assay CV < 12%).Plasma GDF8 was measured with an ELISA kit (R&D Systems, Minneapolis, MN, USA) Catalogue No: DGDF80. The sensitivity was 5.32 pg/mL. Intra-CV = 5.4%, inter-CV = 6%.Plasma total cholesterol (TC) and triglyceride (TG) were measured by enzymatic methods (CHOD-PAP). High-density cholesterol (HDL-C) and low-density cholesterol (LDL-C) were determined using a photometric method (Pars Testee’s Quantitative Detection kit, Tehran, Iran) with a coefficient and sensitivity of 1.8% and 1 mg/dL and 1.2% and 1 mg/dL, respectively.Insulin levels were measured with an ELISA kit (Demeditec, Kiel, Germany) with a sensitivity of 1 ng/mL and between and within CV of 5.1% and 8.4%, respectively. Glucose levels were measured with a colorimetric enzymatic kit (Paracetamol Glucose, Colorimetric Enzymatic kit, Parsazmun, Tehran, Iran) with a sensitivity of 5 mmol/L. HOMA-insulin resistance (HOMA-IR) was calculated from the ratio of insulin to glucose and the HOMA-IR index [HOMA-IR = fasting insulin (mU/L) × glucose (mmol/L)/22.5] [