4.5. Study Strength and Limitations
breast milk
SECONDARY
This study has several notable strengths. Firstly, the implementation of a standardized CF concept ensured consistency and accuracy in assessing PUFA intake, which allowed for precise calculations during the CF period. Additionally, the inclusion of monthly dietary records throughout the first year of life provided comprehensive and continuous data on dietary intake. However, it is important to acknowledge the limitations of this study. As a secondary outcome analysis of a randomized controlled trial, the study was not specifically powered to detect statistically significant associations between dietary PUFA intake and neurodevelopmental outcomes. While the study provides valuable insights, studies designed specifically for this purpose would be needed to establish conclusive associations. Furthermore, a limitation of the study was the reliance on mean breast milk intake data from previous studies due to the unavailability of exact intake data. Despite these limitations, this study enhances our comprehension of PUFA intake in VLBW infants and sheds light on the potential impact on neurodevelopmental outcomes.