Reagents
LYSING, BLOOD, FRANKLIN
Blood collection tubes containing EDTA (pink-top, 6mL vacutainer tubes) and butterfly needles were purchased from Becton-Dickinson (Franklin Lakes, NJ, USA). Attune Focus fluid, Performance tracking beads, wash and shut-down solutions, de-bubble buffer, and the 2 lysing buffers, High Yield Lysing buffer™ and Cal-Lyse™ whole-blood lysing solution, were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Monoclonal antibodies described below were obtained from Thermo Fisher Scientific (Waltham, MA, USA), except the CD25-Brilliant Violet421 antibody that was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Customized Bio-Plex Pro™ human cytokine arrays were purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA).