Transcriptomic analysis
RNA was extracted from cryopreserved PBMCs and sequenced for RNA expression and TCR analysisIndexed libraries were constructed using the SMARTer® Stranded Total RNA-Seq Kit v3 (Takara Bio) following the manufacturer’s instructions. The quantity and quality of the libraries were assessed by Qubit and Agilent 2100 Bioanalyzer, respectively. Libraries’ molar concentrations were validated by qPCR for library pooling. Sequencing was performed on the Illumina HiSeq 4000 platform using PE150 chemistry (Illumina).The raw data were aligned to the hg38 reference genome, and the number of reads per gene was counted using STAR v.2.5.1