Calcium released to the citric acid
dentine mineral loss
To quantify dentine mineral loss, the calcium released to the citric acid after the erosive cycles was measured. For the total calcium release, 1 ml of citric acid after each erosive cycle was mixed per specimen and analyzed with an atomic absorption spectrometer (Aanalyst 400, Perkin-Elmer Analytical Instruments). The measured amount of calcium was normalized to the exposed dentine surface area [