Expansion and characterization of BM-MSCs and SHED
Previously isolated BM-MSCs, under ethical approval from the Regional Committee for Medical and Health Research Ethics in Norway (2013/1248 REK Sør-Øst C), were expanded in Minimum Essential Medium Alpha (αMEM, Thermo Fisher, #32561029) supplemented with 5% human platelet lysate (hPL) and 1% antibiotics (penicillin/streptomycin, Sigma-Aldrich, #P4333) and maintained at 37°C in a humidified incubator containing 5% CO