4.2. Study Protocol
Two experimental models were used: a single application of various curcumin concentrations before a single UVB exposure and daily application of various curcumin doses for 7 days before a single UVB exposure on the seventh day. The mice were randomly allocated to 11 groups, each containing five mice. Group 1 received no treatment, group 2 received only UVB irradiation, and group 3 received acetone and UVB irradiation. Groups 4–7 received topical curcumin applications with concentrations of 0.1 µM, 1 µM, 10 µM, and 100 µM, respectively, with a dose of 2 µL/cm