Sample size calculation
The sample size was determined using the G*power 3.0.10 software. The required minimum sample size was 24 subjects for each group. This demonstrated that a target significance value of 0.05 would need a sample size of 30 subjects (30 test sides and 30 control sides) to have 85% power to detect a statistical difference between the CGF and control sites. Additionally, it was conducted consistent with previous comparable studies (