Evaluation of inflammatory factors
tumor necrosis, Fasting venous blood
TUMOR NECROSIS, -20
Fasting venous blood was harvested before and after treatment from patients in the control and observation groups and tail vein blood from mice in each group, with or without EDTA anticoagulation, centrifuged at 3000 r/min for 10 min, then serum or plasma was separated and stored at -20℃. Serum interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and hypersensitive-C-reactive protein (hs-CRP) levels were estimated by ELISA kits (Nanjing Jiancheng Institute of Biological Engineering, Nanjing, China) [