Primary antibodies
TNF-α
The following primary antibodies were used for western blot analysis: TNF-α (1:500, 52B83; sc -52,746, Santa Cruz), total-nuclear factor-kappa B (NF-κB) (1:1000, #8242; Cell signaling Technology, Danvers, MA, USA), phospho-NF-κB (1:1000, #3033; Cell signaling Technology, Danvers, MA, USA), cyclooxygenase 2 (COX2) (1:1000, #4842S; Cell signaling Technology, Danvers, MA, USA), superoxide dismutase 2 (SOD2) (1:1000, 13141S; Cell signaling Technology, Danvers, MA, USA), heme oxygenase 1 (Hmox1) (1:1000, 70081S; Cell signaling Technology, Danvers, MA, USA), glutathione peroxidase (GPX) (1:1000, B-6; sc -133,160, Santa Cruz), Catalase (1:3000, H-9; sc271803, Santa Cruz), and α-tubulin (1:3000, #2144; Cell signaling Technology, Danvers, MA, USA).