Anti-platelet factor 4/Heparin antibody detection
SECONDARY
The enzyme-linked immunosorbent assay (ELISA) kit for anti-heparin/platelet factor 4 antibodies (Anti-PF4) was purchased from Cloud-Clone Corp. (Wuhan, China). Serum samples were detected strictly according to the manufacturer’s instructions.Standards or samples were added to the microtiter plate wells, which had been precoated with platelet factor 4/Heparin (PF4/H). Then after adding horseradish peroxidase (HRP) conjugated secondary antibodies, TMB substrate solution, and sulfuric acid solution step by step, the wells that contained anti-PF4/H antibodies would change colors to yellow. The concentrations of the anti-PF4/H antibodies were calculated by the optical density (OD) values, which were measured by a spectrophotometer at 450 nm.