Variant selection algorithm
truncating mutations, tumor, Cancer
CANCER, TUMOR, DELETION
Stringent selection algorithm was applied to remove a maximum of irrelevant variants. We considered a minimal allelic ratio of 5% and a maximal frequency in the population of 0.1%. Only truncating mutations (frameshift deletion and insertion, stopgain, splicing alteration and hotspot mutations from Cancer Hotspot database) with a minimal coverage of 200 reads were retained for tumor suppressor gene variants. All missense variants known to be hotspot mutations from Cancer Hotspot database and no minimal coverage were retained for oncogenes variants