2.3. Blood Samples and Analysis
BLOOD
Blood samples were obtained from the antecubital vein after a 12-h fast (both before and after 8 weeks of exercise) and collected into vacutainer tubes with EDTA under the same conditions and time periods. The collected blood was centrifuged at 3000 rpm for 10 min and stored in a deep freezer at −70 °C. The total cholesterol levels and the respective fractions were analyzed using enzymatic colorimetric assays (Modular Analytics Co., Manchester, UK).