Genotyping and genetic risk score
MINOR
Study participants were genotyped for three variants; the patatin‐like phospholipase domain‐containing protein 3 rs738409 C > G (PNPLA3 I148M), the transmembrane 6, superfamily member 2 rs58542926 C > T (TM6SF2 E167K), and the splice variant hydroxysteroid 17‐beta dehydrogenase 13 rs72613567 T > TA (HSD17B13) variant, using TaqMan 5′‐nuclease assays in duplicate (QuantStudio 3; Thermo Fisher, Waltham, MA) (Gellert‐Kristensen, et al., The three genotypes were coded as 0 for noncarriers, 1 for heterozygous, and 2 for homozygous for the risk‐increasing allele. The risk‐increasing allele for PNPLA3 and TM6SF2 was the minor allele, while the risk‐increasing allele for HSD17B13 was the major allele.