2.3.2. Biochemical Outcomes
muscle damage
BLOOD
Blood samples were collected from the antecubital vein into tubes containing EDTA after a 12 h fast. The samples were centrifuged at 3000 rpm for 20 min in a clinical centrifuge. Plasma was separated and stored at −80 °C until further analysis. The levels of plasma glucose and lipid profile (high-density lipoprotein-cholesterol (HDL-cholesterol); low-density lipoprotein-cholesterol (LDL-cholesterol); triglycerides) were measured by spectrophotometry (Advia 2400, Siemens HealthCare Diagnostics, Deerfield, IL,USA). Serum samples were also analyzed using one-step sandwich immunoassays for creatine kinase activity and myoglobin concentration (AU5800 Plus Biochemical Autoanalyzer, Beckman Coulter Inc., Brea, CA, USA) as markers of muscle damage. These outcomes were assessed in each participant at baseline (week 0) and 24 h after the final training session scheduled in the last week (week 16) of the program.