RNAseq processing and analysis
STERILE
The cryovial tubes were retrieved from --80°C and rapidly thawed in a 37°C water bath for 2–3 min. Thawed cells were gently transferred to a 50 ml conical tube containing 8 ml of warm complete RPMI medium (RPMI-1640 medium with 10% sterile heat-inactivated FBS). The tube was then centrifuged at 300