Pretreatment planning and injection of Ad5-yCD/
cancer, prostate
ADENOVIRUS, CANCER, PROSTATE, BLOOD
Informed consent was obtained from all patients before study-specific procedures were initiated. Pretreatment planning included transrectal ultrasound guided needle biopsy of the prostate and computerized tomography (CT) simulation. Transrectal ultrasound scan (TRUS)-guided biopsy cores (≥ 6 cores) were taken to map the location of the cancer within the prostate. Blood was collected for baseline detection of the Ad5-IL-12 adenovirus by polymerase chain reaction (PCR) and serum cytokines (IL-12, IFNγ and CXCL10) by Enzyme-Linked Immunosorbent Assay (ELISA).Men received a single intraprostatic injection of Ad5-IL-12 adenovirus on day 1. The injection was performed on an outpatient basis as described previously [