2.3. Materials
BLOOD
Heparin(Changzhou Qianhong, China), UCB collection bags (Shandong Weigao, Weihai, China), 6% hydroxyethyl starch (Hespan, B. BRAUN, Melsungen, Germany), 1% hemolysin, 7-aminoactinomycin D, CD45-fluorescein isothiocyanate monoclonal antibody, CD34-phycoerythrin monoclonal antibody, and IgG1-phycoerythrin monoclonal antibody were purchased from BD Biosciences (New York, USA). Blood gas electrolyte analyzer(BG-800E, Meizhou Kangligao), Medical cryogenic centrifuge (RC 3BP+, Thermo Scientific), automatic blood analyzer (XE-5000, Sysmex), flow cytometer (FACSCalibur, BD), inverted microscope (CK40 and CKX41, OLYMPUS), and Automatic Microbial Detection System (VersaTREK, ThermoFisher) were used in the study.