Biochemical and hormonal analysis
PARATHYROID, BLOOD
Blood samples were collected from participants in a clinical laboratory while the subjects were fasted for serum 25(OH) vitamin D, calcium, phosphorus, Parathyroid hormone (PTH), alkaline phosphatase (ALP), and creatinine at baseline (two weeks after transplant) and six months after transplant. Serum levels of 25(OH) vitamin D (reference range [RR]: 20–80 ng/ml) were measured by high-performance liquid chromatography (Young Lee 9100, South Korea). An A25 auto-analyzer (Biosystems SA, Barcelona, Spain) was used to measure calcium (RR: 8.5–10.5 mg/dl), phosphorus (RR; 3.7–5.4 mg/dl), and ALP (RR: 44–147 U/L) levels. Based on sandwich technology, PTH levels (RR: 10–65 ng/L) were checked with ELISA kits by MyBioSource company (USA). eGFR was calculated according to The CKD-EPI equation, expressed as GFR = 141 * min(Scr/κ,1)α * max(Scr/κ, 1)-1.209 * 0.993Age * 1.018 [if female] * 1.159 [if black].