Validation of the cuproptosis‑related lncRNA signature
cancer
CANCER, PROSTATE
Trizol reagent (ComWin Biotech, Beijing, China) and reversed transcribed into cDNA with the TransScript First-Strand cDNA Synthesis SuperMix kit (TransGen Biotech, Beijing, China) was used to extract total RNA from prostate normal or cancer cell lines RWPE-1, PC3, DU145, VCaP, and LNCaP according to the manufacturer’s description. Real-time quantitative polymerase chain reaction (RT-qPCR) was conducted in triplicate with qPCR SYBR Green SuperMix (TransGen Biotech, Beijing, China). β-Actin was employed as an internal reference gene to normalize relative expressions of lncRNA with the 2