2.3. Materials
STERILITY
Trace element-free sulfuric acid, sodium hydroxide (BDH Inc., Toronto, ON, Canada) and high-purity distilled deionized water filtered through a 0.22 mm Millipore filtration system (Millipore, Bedford, MA, USA) were used throughout the preparation of the oral doses of Zn isotopes. All laboratory work was completed in a clean laboratory under a laminar flow hood. Acid-washed glassware and plastics were used throughout. Isotopes of The isotopes complexes were tested for sterility and pyrogenicity by the pharmacy of INRCA and encapsulated in gelatin. The oral capsules had a final total amount of 2.0 mg of 