Clinical and immune response monitoring during vaccination and follow-up
CRC
The detailed timeline presentation of clinical treatments, vaccinations, and clinical outcomes is shown in Fig. Clinical outcome and immune response monitoring in the enrolled CRC patients during vaccination and follow-up. During vaccination and follow-up, in vitro IFN-γ ELISpot assay was performed to monitor immune response by using autologous PBMCs stimulated by neoantigen pools or each neoantigen individually. IFN-γ ELISpot assay indicated that our standard vaccination protocol is efficient in the induction of antigen-specific immune response in CRC patients. As shown in Fig. 