2.7. Statistical Analysis
Descriptive statistics (means ± standard deviation) were used to summarize all data. The normality of the data was assessed by the Shapiro–Wilk test. A two-way ANOVA repeated measures test was used to determine group X time interactions. One-way ANOVA and Fisher LSD post-hoc tests were used for evaluation of baseline data for the four groups. When a significant difference was detected by ANOVA, mean differences were determined by pairwise comparisons. The sample size needed to detect a statistical difference between study variables with a 95% confidence interval (CI) and equal to or greater than 80% of the power value was calculated. Effect sizes (ES) were reported as partial eta-squared, where ES were considered trivial (<0.2), small (0.2–0.6), moderate (0.6–1.2), large (1.2–2.0) and very large (2.0–4.0). A 