DNA sequencing of baseline TURBT specimens
Archival baseline TURBT tissue underwent tumor-only targeted DNA sequencing using the Illumina NextSeq platform (Caris Life Sciences). An Agilent custom-designed SureSelect XT assay (Caris MI TumorSeek 592-Gene NGS Panel) was used to enrich 591 whole-gene targets. Sequencing and gene variant calling were carried out as previously described; the pipeline automatically filters out known common germline population variants (that is, from databases such as dbSNP) and flags pathogenic mutations that are potentially germline