scRNA-Seq and paired single-cell TCR-Seq.
PBMCs were resuscitated and subjected to scRNA-Seq on the 10x Genomics platform. The 10x Genomics single-cell 5′ reagent kit was coupled with TCR V(D)J analyses to profile TCR clonotypes at a single-cell resolution. scRNA-Seq reads were aligned to the hg19 human reference genome using “CellRanger count” (v3.0.2), and scRNA-TCR data were aligned to the vdj-GRCh38 reference genome.