Differential changes of splicing variants analysis linear over time
Since previous studies suggested that CR exerts part of its beneficial effect by regulating the expression of alternative RNA splice forms, we took advantage of the extreme depth of our paired RNA‐Seq to investigate differential transcript expression and differential transcript usage over time between CR and AL, which can be used as proxy measures for splicing variants. To enhance the reliability of our analysis, we relied on two widely used analytical methods, Kallisto and RSEM (Bray et al., We primarily focused on the 349 splicing variants (from 328 genes) that had been identified in both Kallisto and RSEM either by differential transcript expression (179 splicing variants from 174 genes; Table Significant selected pathways (In further analyses, we focused on the 80 splice variants from 78 genes identified in both differential transcript expression and differential transcript utilization analyses (Table Differentially transcript expression change and/or differentially transcript usage changes of twelve mRNA splicing variants over time between CR and AL. Examples of splicing variants identified as showing significant differential expression changes/usage at Interestingly, our analyses also pointed to differential transcript expression and/or differential transcript usage for some splicing variants at 