Cardiometabolic profile
Metabolic Syndrome
BLOOD, METABOLIC SYNDROME
The systolic and diastolic blood pressure were measured on three different days with an automatic monitor (Omron Healthcare Europe B.V. Hoofddorp, The Netherlands), and the average was used in later analyses. We calculated the mean blood pressure as diastolic blood pressure + 1/3(systolic blood pressure − diastolic blood pressure). Blood samples were collected from the antecubital area in the morning after fasting for > 10 h. All samples were centrifuged, and aliquots of serum were stored at − 80 °C until analysis. All participants were requested to abstain from drugs and caffeine and to avoid moderate-intensity physical activity and vigorous-intensity activity for 24 and 48 h before testing, respectively. Serum glucose, total cholesterol, HDL-C and triglycerides were assessed following standard methods using an AU5832 automated analyzer (Beckman Coulter Inc., Brea CA, USA). LDL-C was then estimated [Cardiometabolic risk scores were calculated for each sex based on variables included in the diagnosis of Metabolic Syndrome [