4.1. Limitations
EVENTS, PHYSIOLOGICAL STRESS
While this study concentrated on the distinct serum levels of amino acids following an ultramarathon race and the potential impacts of vitamin D supplementation, it has certain limitations. Despite our meticulously designed procedures, we were unable to analyse the concentrations of methylarginines in the subjects’ urine. These urinary levels could have provided further insight into the efficiency of methylarginine elimination by the kidneys and offered an enhanced understanding of their metabolism and renal function. However, the data we gathered significantly elucidate the range of pathophysiological processes associated with these compounds, particularly in the context of the physiological stress induced by an ultramarathon. This study provides substantial value for understanding physiological responses to extreme endurance events with respect to these compounds and their role in extreme physical exertion.