Receptor binding assay
To test the ability of UP165 to bind to the melatonin binding site of the melatonin receptor 1 (MT1) and 2 (MT2) melatonin receptors, membrane-bound human MT1 and MT2 melatonin receptors were incubated with UP165 in the presence of [Cell membranes from human recombinant Chinese hamster ovary (CHO)-K1 cells expressing the human melatonin MT1 receptor were harvested and incubated with UP165 at concentrations 0.78, 1.56, 3.12, 6.25, 12.5, 25, 50, 100, 200, and 400 