Study population and sample size
Patients were selected from the population referred to the Periodontal Clinic at School of Dentistry of Ribeirao Preto – University of Sao Paulo (FORP/USP, Ribeirao Preto, SP, Brazil). Patients who fulfilled the inclusion criteria were invited to participate in the study. All eligible patients were thoroughly informed of the nature, potential risks, and benefits of their participation in the study and signed a Term of Informed Consent. The study protocol was reviewed and approved by the Research Ethics Committee at FORP-USP (protocol number: 68692917.5.0000.5419) and registered at Brazilian Clinical Trials Registry (protocol number: RBR-59v2yb). The study was conducted in accordance with the Helsinki Declaration of 1975, as revised in 2013.The sample size was determined using the software Graphpad Statemate 2.0 (GraphPad Software, Inc., San Diego, CA, USA). The ideal sample size was calculated to ensure an 80% power to recognize a significant difference of 10% in BoP (δ) between the groups analyzed with a confidence interval of 95% (