Experimental design
LIVER
On the study day (Figure Study Design (1) Protocol Study Day: Participants were randomized to either remain fasting or consume a liquid meal. Peripheral blood samples were taken at several timepoints. Transjugular liver biopsies were performed after 60 min, and blood from the liver vein was collected. (2) Liver histology: Two expert pathologists scored biopsies and evaluated NAS scores. (3) Hormones and metabolites: We measured glucose, insulin, and C‐peptide at all timepoints. (4) Immunoassays: We measured glucagon and FGF21 at all timepoints in peripheral blood and in liver vein blood at 60 min. (5) Genotyping: We analyzed the genotypes of PNPLA3, TM6SF2 and HSD17B13. Figure created with 