METHOD
sunburn, ±, diabetes, allergies, trauma
SKIN, HYPERTHYROIDISM, ALLERGIES, HYPOTHYROIDISM, HYPERTENSION, DISEASES, DIABETES
This clinical trial was approved by the ethics committee of our hospital, and the clinical trial was duly registered.The test was conducted from September 27th 2022 to January 16th 2023, and the panelists include 61 young (18–35 years old) and 67 elderly (45–65 years old) groups, with their skin types covering oily, normal and dry. The oral administration level of HA was designed as 0 mg/day (placebo, 100% Erythritol), 100 mg/day (95% Erythritol, 5% 300 KDa HA), and 200 mg/day (90% Erythritol, 10% 300 KDa HA). The stratified randomization method was employed to divide the panelists into three different administration groups, whose population is as shown in Table Experimental design.Young groupDry0 (placebo)6Young groupDry1008Young groupDry2007Young groupOily0 (placebo)6Young groupOily1006Young groupOily2006Young groupNormal0 (placebo)8Young groupNormal1007Young groupNormal2007Elderly groupDry0 (placebo)9Elderly groupDry1007Elderly groupDry2006Elderly groupOily0 (placebo)6Elderly groupOily1009Elderly groupOily2007Elderly groupNormal0 (placebo)7Elderly groupNormal1009Elderly groupNormal2008Prior to the test, the participants were thoroughly screened by doctors to ensure that they had no skin‐related diseases, normal liver and kidney function, negative results on blood and urine tests, and negative HCG (reproductive age). Additionally, they had no known allergies to the tested product components and were not suffering from uncontrolled diseases such as diabetes, hypertension, hyperthyroidism, or hypothyroidism. The subjects did not use any dietary supplements containing collagen, hyaluronic acid, chondroitin sulfate, or any combination thereof within 3 months before the test, did not undergo any skin surgery or medical beauty procedures within 12 months before the test, and did not experience any skin trauma or sunburn within 3 months before the test.The HA drinks used in the test were a unit dose solid beverages provided by Bloomage Biotech Co., Ltd. The HA ingredient in the formula was a food‐grade material prepared by fermentation. The panelists were required to dissolved one unit dose in 200 mL of water and consumed it every day. Also, they were required to ensure the total amount of daily water intake is 1.2∼1.4 L. During the test, the same facial cream products, that is, the same brand and lot#, were given to the panelists as their only skin care product for daily use. To be noted, this facial cream did not contain any active ingredients such as HA.After oral administration of HA for 2, 4, 8, and 12 weeks, the CorneometerCM825 (Courage & Khazaka, Germany) was used to measure Skin hydration. Ultrasonic skin diagnostic instrument (UC22, Courage&Khazaka, German) was used to measure Skin thickness and density. Visia‐CR (Canfield Scientific, Inc. USA) was used to test Skin appearance. Chromameter CM2600D (KONICA MINOLTA, Japan) was used to detect Skin tone (ITAo). The measurements were carried out in the clinical lab with controlled temperature and humidity of 22 ± 1°C/RH 50% ± 10%. Prior to measurements, the panelists were instructed to sit quietly in the lab for at least 30 min.Skin tone is quantified by ITA