Histology
SE, tumor, Embryonal rabbit kidney tissue
TUMOR
Histological and immunohistochemical analyses of all cases were performed in the central histology lab at BioNTech SE on representative tissue slides of formalin-fixed, paraffin-embedded neoplastic tissues. In brief, slides were stained with hematoxylin and eosin on a Leica ST5020 multistainer and manually stained using a monoclonal mouse anti-human CLDN6 antibody (CLAUDENTIFY6, BioNTech Diagnostics) and a normal control reagent. Embryonal rabbit kidney tissue served as a positive control for each immunohistochemical staining. All samples were analyzed by board-certified pathologists regarding tumor content (hematoxylin and eosin) and CLND6 expression in neoplastic cells. CLDN6 staining positivity was classified as negative (0), weak intensity (1+), medium intensity (2+) and strong intensity (3+). Only membranous staining was considered.