Measurement of blood parameters and vitamin D
CLOT
Following an overnight fasting, venous blood drawing took place between 6:30 and 9:00 in the morning. Two EDTA-stabilized blood collection tubes containing 8 mL each (Vacuette, Greiner bio-one, Kremsmünster, Austria) were kept for 30 min at room temperature and then transported to the Laboratories of the Department of Nutritional Sciences of the University of Vienna for harvesting PBMCs that were further subjected to the CBMN assay as described above. Further, Serum Sep Clot Activator collection tubes (Vacuette, Greiner bio-one, Kremsmünster, Austria) were filled with 8 mL venous blood that was used to assess vitamin D3 serum levels. After 30 min at room temperature, the clotted blood was centrifuged at 3000 rpm for 10 min at room temperature. In order to analyze 25(OH)D2 and 25(OH)D3, the Access 25(OH) Vitamin D Total assay (Beckman Coulter Austria, Vienna) was used. Right after blood drawing, the blood collection tubes were transported into a routine medical laboratory (Dr, Claudia Vidotto study lab GMBH, 1230 Vienna) to measure vitamin D serum levels, plasma hs-C-reactive protein (hs-CRP), hs-Troponin and general blood parameters including erythrocytes, hematocrit and hemoglobin status.