RNA extraction and quantitative PCR for SARS-CoV-2
SARS-CoV-2 infection
SARS-COV-2 INFECTION
Viral RNA was extracted from 100 µL of nasopharyngeal or oropharyngeal swab, using the automated extractor, EXTRACTA 32 (Loccus, Cotia, Brazil), and magnetic bead extraction kits (MVXA-P016 FAST), and following the manufacturer’s instructions (Loccus, Cotia, Brazil). A negative extraction control (UltraPure DNase/RNase-Free Distilled Water, Thermo Fisher Scientific, Waltham, MA, USA) was added to each extraction run.The diagnosis of SARS-CoV-2 infection and monitoring of VL were performed by real-time reverse transcription-polymerase chain reaction (RT-PCR) using fluorescent probes (Taqman) [