Allele-specific oligonucleotide polymerase chain reaction for detection of minimal residual disease
SEPARATION
For MRD analyses, DNA was extracted after density gradient separation of lymphocytes from bone marrow (BM) aspirates, which was then stored at −20 °C until analysis. We used patient-specific quantitative allele-specific oligonucleotide PCR (qASO-PCR) assays on immunoglobulin heavy chain (IgH) and kappa/lambda (k/λ) light chain as recently described [