2.9. Biochemical Analysis
MetaboLead RemL-C, Clinimate
BLOOD
The concentrations of TGs in serum were determined using an enzymatic colorimetric assay (Pure Auto TG-N; Sekisui Medical, Tokyo, Japan). The measurement of phospholipids in serum was based on the choline oxidase DAOS (N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5 dimethoxyaniline sodium salt) method (L-Type Phospholipids; Wako, Osaka, Japan). Quantitative analysis of total cholesterol was performed using an enzymatic colorimetric assay (Cholestest CHO; Sekisui Medical, Tokyo, Japan). The serum concentrations of βLPs were measured using a turbidimetric assay (Clinimate β-L; Sekisui Medical, Tokyo, Japan). A direct method was used to determine the serum concentrations of HDL cholesterol (Cholestest N HDL; Sekisui Medical, Tokyo, Japan). An enzymatic colorimetric assay (Cholestest LDL; Sekisui Medical, Tokyo, Japan) was employed to quantify LDL cholesterol in serum. The above assays were performed on a Labospect 008 automatic biochemical analyzer (Hitachi, Ibaraki, Japan). Non-esterified FFAs in serum were quantitatively determined using an enzymatic assay based on the ACS-ACOD (acyl CoA synthetase–acyl CoA oxidase) method (NEFA-HR (2); Wako, Osaka, Japan). RLP cholesterol levels were measured using a homogeneous assay (MetaboLead RemL-C; Kyowa Medex, Tokyo, Japan). Measurements of FFAs and RLP cholesterol were conducted on a JCA-BM8040 automatic analyzer (JEOL, Tokyo, Japan). Blood glucose was measured based on the kinetic-spectrophotometric method (CicaLiquid Glu J; Kanto Chemical, Tokyo, Japan) by using a JCA-BM9130 automatic analyzer (JEOL, Tokyo, Japan).