rs-fMRI date acquisition
BRAIN
All images were obtained on a Siemens 3.0-Tesla signal scanner (Siemens, Verio, Germany) with a standard head coil, at Nanjing Brain Hospital. During the scanning process, foam pads and earplugs were used to reduce head movement and noise, respectively. Participants were instructed to close their eyes, stay awake, and not engage in any mental exercises. A gradient-recalled echo-planar imaging pulse sequence was used for rs-fMRI date acquisition, with the following settings: repetition time (TR)/echo time (TE) = 3000 ms/40 ms, flip angle = 90°, slice thickness = 4.00 mm, field of view = 240 mm×240 mm, and matrix size = 64 × 64. The scan lasted 5.06 min. A high-resolution T1-weighted magnetization prepared gradient echo image was obtained for each participant, for spatial normalization and localization, with the following parameters: TR = 1900 ms, TE = 2.48 ms, slice thickness = 1.00 mm, gap = 0.5 mm, slice number = 176, and matrix size = 256 × 256. The resting-state scan lasted 4.3 min. Rs-fMRI data were collected from the rMDD and HC groups at baseline. Six-month follow-up rs-fMRI data were collected for only the rMDD group.