Blood sampling and biochemical measurement
7-milliliter venous blood sample
At the beginning and the end of the intervention, after 10–12 h of fasting, a 7-milliliter venous blood sample was collected from each individual and centrifuged (2000 g for 10 min) to isolate the serum. The samples were kept at -80° C until biochemical analysis.In the present study, the primary outcome was the determination of variations in the serum ghrelin concentration from the baseline to the end of the intervention. Serum ghrelin concentration was measured by ELISA kits (Crystal day, China, cat. No: E3091Hu) before and after the intervention.