Cross-reactive IgG antibodies against non-vaccine VLPs
Plant-produced VLPs of GII.4 OC08154 (2008), GII.4 Washington (2018), and GII.6 Maryland (2018) (Icon Genetics GmbH) were coated onto separate 96-polystyrene plates (Nunc MaxiSorp) by adding 100 µl per well of a coating solution (4 µg/ml). Measurement was performed as described for IgG ELISA in (The evolutionary history of VP1 protruding domains of norovirus VLPs used in ELISA assays was inferred using MEGA with the Neighbor-Joining method on an alignment generated with MUSCLE (