T cell clone definition
We identified and tracked T cell clones by their β chain sequence (TRB), defined as the nucleotide CDR3 sequence (including the conserved C and F residues) and a deterministic V and J gene alignment. For T cells identified by single-cell sequencing, we similarly defined clones by the TRB sequence to map clones to paired TCR Vβ sequencing. Owing to the higher entropy of the nucleotide CDR3 sequence generation probability distribution