2.2. Participants
A total of 62 subjects of both sexes, aged between 40 and 64 years, were included. In order to participate in the study, they had to meet all the following selection criteria: age between 40 and 65 years; Body Mass Index (BMI) between 25–30 kg/mIn order to participate in the study, all subjects followed some hygienic-dietary recommendations that were explained by the investigators prior to the beginning of the study: not to start or modify any hormonal treatment during the study if it was not duly justified; not to significantly modify dietary and physical activity habits; not to take or follow any treatment that could affect the study parameters. Lifestyle questionnaires (GPAQ or Global Physical Activity Questionnaire) [To carry out the blood extractions, the following instructions were set: subjects had to come after fasting for a minimum of 12 h, only allowing the intake of water in the 3 h prior to the extraction and they could not perform moderate-intense exercise at least 24 h prior to blood collection.