2.3.1. Metabolic Profiling
non-fasting venous blood
Sample Storage and Preparation. 2–3 mL of non-fasting venous blood was drawn into sodium heparin vacutainer tubes, shipped at ambient temperature to the laboratory, and received within 24 h of collection. Plasma was obtained by standard centrifugation procedure, dispensed into small plastic vials, and stored at −80 °C until analysis. Plasma samples were shipped frozen for analysis to Metabolon, Inc. (Durham, NC, USA). A total of 201 plasma samples were analyzed belonging to the treatment groups listed in Metabolomic profiling was performed as described previously [