Passive force measurements in isolated cardiomyocytes
Cardiac muscle
OBESE
Given the close relationship between the overall cardiac function in vivo and the viscoelastic composition of individual cardiomyocytes, we conducted experiments on permeabilized cardiomyocytes as a surrogate for diastolic function at the cellular level. Cardiac muscle resists lengthening by exerting an extending force in response to stretch at nominal calcium conditions, that is, FOral GGA treatment reduces myofilament stiffness in obese ZSF1 rats. Myofilament stiffness was measured as increases in passive force (FTreatment of permeabilized cardiomyocytes with recombinant HSPB5 or HSPB1 abrogated the differences in FRecombinant human HSPB5 (0.01 mg/mL) and human HSPB1 (0.01 mg/mL) reduce stiffness of permeabilized cardiomyocytes of obese ZSF1 rats and abrogate the effect of oral GGA treatment. Stiffness was measured as the passive force (F