DMPA-IM initiation induced an enrichment of T cell gene signatures in the FGT
RECRUITMENT
The use of DMPA-IM has been reported to induce a diverse array of biological effects in the FGT. A closer examination of the 151 DEGs identified in DMPA-IM arm (Supplementary Table 2) showed significant alterations in a handful of genes that function in T cell activation (PRF1, TNFSF18, MAF) and tissue recruitment of immune cells (CXCL5, VCAM1). As a more sensitive approach to identify enriched gene pathways associated with contraceptive initiation, we performed Gene Set Enrichment Analysis (GSEA) using various well-defined gene-set/pathway collection databases from the Molecular Signatures Database (MSigDB) (DMPA-IM initiation induced an enrichment of T cell gene signatures in the cervicovaginal tract (nominal 