4.3. Experimental Design and Blood Sample Collection
A crossover design was considered for the clinical trial, so that each subject serves as his/her own matched control, thus blocking the variability due to the subjects. This design allows for a smaller number of participants in the study than in a parallel one while ensuring the same statistical power. All 11 participants were cited on two different days, one for FDJ consumption and another one for SDJ, in different time periods separated by 15 days. Each day, fasting blood samples were obtained from each participant. They were then given 400 g of the juice to be ingested with no meal. After 4 h, during which they were not allowed to eat anything, a second blood draw was performed. Two blood samples from each participant were collected into BDVacutainer SSTII Advance Tubes (REF 367953), which were allowed to coagulate for 30 min and after centrifuged at 20 °C and 1500× 