3.3. Design of Weighted Sub-Scores That Contain the CpG Sites of Each Diet and the Calculation of the Total Methylation Score for the Prediction Model
REGRESSIONS
Weighted sub-scores were made for each diet, using the sum of the previously selected CpG sites and multiplying them by the beta coefficients obtained in each of the multiple linear regressions of the MHP diet and the LF diet (To obtain a total score for each individual that would allow to be included as a term for the interaction with the diet variable, the MHP diet sub-score was subtracted from the LF diet sub-score, as shown in A linear mixed effect model was used to predict, based on the total methylation score of each individual, which diet will be the best for the volunteers based on the greatest percentage of BMI loss. Therefore, a linear mixed effect model was designed with the percentage of BMI loss as the dependent variable and total score, diet (MHP/LF), and the interaction term between the total score and the diet as a fixed effect. Moreover, the IDs of the participants were included as a random effect. The model was adjusted for sex and age. 