2.3.7. Inflammatory Factors and Blood Cell Count
At baseline and immediately after the constant load exercise, 5 mL of venous blood was collected from the anterior elbow vein of each subject using standard venipuncture technology. Plasma was loaded into the green blood vessel of a sodium heparin anticoagulant for the blood cell count test. Serum loaded in the red blood collection vessel of a procoagulant was centrifuged at 4000 r/min for 10 min to remove the supernatant. The tubes were stored in a refrigerator at −80 °C until further analysis. The levels of IL-1β, IL-4, IL-6, IL-8, IL-10, TGF-β1, and IgA were determined using an ELISA kit provided by Shanghai Jianglai Biotechnology Co., Ltd. (Shanghai, China) and Wuhan Eliot Biotechnology Co., Ltd. (Hubei, China). The blood count indicators—white blood cell (WBC), red blood cell (RBC), haemoglobin level (Hb), hematocrit (HCT), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)—were measured using an automatic haematology analyser (BC-2800Vet, Mindray Medical International Ltd, China).