Protein array
PMT
The protein was extracted from plasma using a protein extraction buffer (Fullmoon biosystems, Sunnyvale, CA) and protein expression was analysed using antibody microarray analysis (Fullmoon biosystems) according to the manufacturer’s protocol. In brief, 50 μg of the protein sample was labelled and incubated with a coupling mixture on the antibody microarray slide (Fullmoon biosystems) and detected with Cy3-streptavidin (GE Healthcare, Chalfont St. Giles, UK). The slide was rinsed and scanned using GenePix 4100 A (Axon Instrument, USA) at a 10 μm resolution, optimal laser power, and PMT. After obtaining the scanned images, they were grided and quantified with GenePix 7.0 Software (Axon Instrument, USA). The data about protein information was annotated using UniProt DB.