Biochemical assays and insulin sensitivity indicators
inflammation
INFLAMMATION, INSULIN SENSITIVITY
Serum glucose was measured by the colorimetric enzymatic method (Randox manual gl2623). Serum insulin concentration was assessed by the immunoezymatic (ELISA) method (Demeditec diagnostics, Germany, no. DE2935). The maximal intra-assay coefficient of variability (CV) was 2.6–1.8%, and the inter-assay CV was 2.9–6.0%. The assay sensitivity was 1.76 µIU/ml. The following formulas calculated insulin sensitivity according to QUICKI and HOMA-IR: QUICKI = 1/(log(fasting insulin [µU/ml] + log(fasting glucose [mg/dl])), HOMA-IR = (fasting serum insulin µU/ml × fasting plasma glucose mmol/l/22.5). The updated computer HOMA2 model was used to determine the β-cell function (HOMA-%B) and insulin sensitivity (HOMA-%S) from paired fasting glucose and insulin level. The HOMA-indexes were obtained by the software HOMA 2 Calculator, version 2.2.3, copyrighted by The University of Oxford. In order to assess if the inflammation status has modified exercise-induced response, the systemic immune-inflammation index (SII) was calculated based on the formula proposed by Chen and co-workers: SII = (P × N)/L, where P, N, and L refer to peripheral platelet, neutrophil, and lymphocyte counts