Reference profile of cell types in deconvolution
The reference profile of a cell type in a single scRNA-seq dataset was the average expression profile across all single cells for this cell type in non-log TPM space. The marker genes for a cell type were defined with the following two steps: (1) carrying out differential expression analysis through the R package limmaThe combined reference profile of a cell type was generated by averaging the reference profiles of this cell type in all scRNA-seq datasets collected in Supplementary Table 