2. Materials and Methods
The study was designed to compare the impact of fasting with and without an initiating bout of exercise on appetite hormones (PYY, PP, GLP-1, GIP, insulin, ghrelin, and leptin). To accomplish this purpose, we used a randomized crossover design with conditions counterbalanced. All participants were exposed to two treatment conditions. Both conditions took place on identical days of the week, and the washout between conditions was at least six days (the longest washout was 13 days). Both intervention days were identical, with the exception of the addition of an exercise bout that took place 30 min into the fast and after the standardized meal [Prior to beginning the study, randomizer.org was used to randomly assign the condition order to participant number [