Immunogenicity endpoints
POLIO
Titers of type-specific NAb on Days 1 and 29 post-vaccination (serum samples collected prior to vaccination and challenge, respectively) measured by standard micro-neutralization assay methods Intestinal immunogenicity was measured as poliovirus fecal neutralization and fecal IgA in samples obtained at screening before vaccination, and then on Days 8, 29 (prior to challenge), 36, 43, 50, and 57 using standardized methods. Fecal neutralizing activity was measured by limiting dilution inhibition of luciferase-expressing wild-type-derived polio pseudoviruses and expressed as the titer needed to achieve 60% neutralization (titers >2 were considered detectable) Responses of type-specific poliovirus antibody-secreting cells (ASC) measured by a standard method 