Hematoxylin eosin staining technique
112.41±26.34
The mean of the formed bone in both groups was significantly higher 6 months after the surgery. The bone area between the control (13.90±8.92) and CGF (13.01±6.52) groups was not significantly different before surgery and 6 months after surgery no significant difference was observed between the control (21.82±13.11) and CGF (26.57±15.04) groups. The area of newly-formed bone in the control and CGF groups was not significantly different, although the amount of newly-formed bone was higher in the CGF group (Evaluating the percentage of formed bone (new bone relative to the former bone) 6 months after surgery using the alizarin red method, the percentage of formed bone in the CGF group (112.41±26.34) was significantly higher than in the control group (64.99±24.96) (P<0.05). Furthermore, in the hematoxylin-eosin method, the percentage of formed bone in CGF (96.16±24.49) was significantly higher than in the control group (60.16±16.39) (P<0.05) (The percentage of formed bone, remaining material, and connective tissue in control and intervention groups using two staining techniques.P-value: Paired Sample T Test; * Wilcoxon Test.There were significant differences in the amount of remaining material in the alizarin red staining technique between the control and CGF groups, being zero in CGF and significantly lower than in the control group with 41.66±35.51 (P<0.05). In the hematoxylin-eosin technique, the amount of remaining material showed a significant difference in both groups, such that it was zero in CGF and significantly lower than in the control group with 0.22±0.6 (P<0.05) (There were no significant differences in the amount of connective tissue between the control (2.29±4.75) and the CGF group (2.75±5.09) in the alizarin red method. Furthermore, there were no significant differences in the amount of connective tissue in the control (0.54±0.54) and the CGF group (0.39±0.47) in the hematoxylin-eosin technique (