2.4. Quantitative Real-Time PCR
For plasma samples, small RNA was extracted using the miRNeasy Serum/Plasma Kit according to the manufacturer's instructions, and 5.6 × 10Total RNAs from cultured cells or PBMCs were extracted using TRIzol Reagent and reverse-transcribed into cDNA using HiScript II Q RT SuperMix (Vazyme Biotech, Nanjing, China). For miRNA detection, cDNA was synthesized with 