Blood sampling and measurements
TG
BLOOD
The subjects attended the two sessions at the laboratory in the morning between 8:00 and 9:00 am, 4 weeks apart. They were asked to consume a standardised meal box by 8:00 pm the night before the test and to not eat or drink anything other than water after that. Blood samples were collected from the subjects before and at 0⋅5, 1, 2 and 4 h after consuming either beverage. Blood glucose concentrations were measured by the hexokinase UV method. Serum insulin and folate concentrations were measured by a chemiluminescent enzyme immunoassay. Serum vitamin C (total ascorbic acid) was measured by high-performance liquid chromatography. TC, high-density lipoprotein cholesterol (HDL-C), triglyceride (TG) and non-esterified fatty acid (NEFA) were measured by enzymatic assays. LDL cholesterol (LDL-C) was calculated using the Friedewald formula. The above biochemistry tests were performed by SRL Inc. (Tokyo).