2.6.1. Resistance Exercise Study
After sampling, 2 mL of blood was injected in K2EDTA-containing Venoject for DNA extraction (TIANamp Genomic DNA) to study the Single-Nucleotide Polymorphism (SNP) in The thermal protocol for PCR operation consisted of step (1) the primary denaturation for 10 min at 94 °C, step (2) second denaturation for 1 min at 94 °C, step (3) annealing for 1 min at 65 °C, step (4) extension for 1 min at 72 °C (steps 2 to 4, 32 cycles), and step (5) final extension for 5 min at 72 °C.In order to verify that PCR was performed in all samples, the internal control was used, in which two primers of mitochondrial genome (namely L strand: 5′-CTCCACCATTAGCACCCAAAGC-3′ and H strand: 5′-CCTATTTGTTTATGGGGTGATG-3′) were used to produce a 250 bp fragment. All samples were run in duplicate with two negative controls.