Cell cycle analysis
EVENTS
Detached cells were fixed and permeabilized in 70% ethanol. Fixed cells were first treated with RNAse A (150 μg/mL) and then cellular DNA was stained using propidium iodide (8 μg/mL; Sigma, St Louis, MO, USA). Samples were analysed on the BD Accuri flow cytometer (BD Biosciences, Stockholm, Sweden) and 30,000 gated events (single cells) were collected for analysis using FlowJo V10 (FlowJo; LLC, Ashland, OR, USA), as previously described [