Sample size calculation
microfilaraemia
This clinical trial was a proof-of-concept study without predefined statistical hypothesis testing between groups. A total of 42 patients were planned to be recruited to ensure adequate precision of the primary outcome, i.e. microfilarial density reduction, based on the natural variability of microfilaraemia. A total of 12 evaluable patients were planned for each of the three intervention groups, in addition to 6 patients in the control group, thus resulting in an overall treatment allocation ratio of 1:2:2:2. Assuming a 50% proportion of patients achieving a microfilaria reduction below 100/ml, this sample size allowed a precision of the point estimate with a 95% confidence interval ranging from 25–75%.