Day 3 embryos
The thaw media was pre-warmed in the incubator at 37 °C. The vitrified embryos were removed from the liquid nitrogen and the outer straw was removed immediately. The containing media in the inner straw was gently poured into thaw media 1 which contained 1 M sucrose in PBS. After 60 s in thaw media 1, the embryos were transferred to thaw media 2 which contained 0.5 M sucrose in PBS. Three minutes later, the embryos were put in thaw media 3 containing PBS only for another five minutes