Primary and secondary outcomes.
skin erythema
INFLAMMATION, SECONDARY, INFLAMMATION, SKIN ERYTHEMA
The primary outcome was a composite outcome composed of clinical and molecular measures of inflammation. The clinical components of the primary outcome were skin erythema by CR300 Chroma Meter, skin thickness by Mitutoyo 9 mm dial caliper, and blinded histopathologic assessment of skin tissue biopsies by a broad-certified dermatopathologist. The molecular components of the primary outcome were the significant changes in RNA and protein expression by bulk-RNA-Seq and protein quantification (Olink Inflammation panel proximity extension assay). Primary outcome measures were collected as specified above.As secondary outcomes, we recorded serum levels of the 3 major VitD metabolites (25[OH]D