4.4.2. Exoquick ULTRA EV Isolation Kit
Small EV isolation with the Exoquick ULTRA EV Isolation Kit for Serum and Plasma (EQULTRA-20A-1, System Biosciences, Palo Alto, CA, USA) was performed according to the manufacturer’s protocol. In brief, sequentially centrifuged plasma (FPS) was filtered through 0.2 μm surfactant-free cellulose acetate filters (16534, Sartorius, Goettingen, GER), 250 μL of plasma was used as sample volume, and 67 μL ExoQuick was added, mixed well by inverting, and incubated at 4 °C for 30 min. Centrifugation of the plasma-ExoQuick samples at 4 °C and 3000× 