4.3. Qualitative Analysis of the Histological Bone Biopsy Samples Was Performed
dehydration
DEHYDRATION, DECALCIFICATION
A standardized protocol for histological preparation of mineralized bone samples was applied to the samples, which included the following: fixation in a 4% formaldehyde solution (BioGnost Ltd., Zagreb, Croatia), dehydration in increasing alcohol concentrations (75%, 85%, 95%, and finally 100%), decalcification with ethyldiaminetetraacetic acid (EDTA, Osteomoll