4.4. Methodological Considerations and Potential Implications
VASODILATION
Several limitations should be considered when interpreting our findings. First, blood flow or vascular conductance as indexes of vasodilation in the working muscles were not evaluated. However, it may be difficult to precisely and continuously measure blood flow in the working muscle during leg cycling (e.g., at the femoral artery). Further, our exercise mode (leg cycling) may be more practically relevant than a local exercise, such as a handgrip or knee extension exercise, which has been conducted with the measurement of blood flow in previous studies [