1. Introduction
death, hyperlipidemia, CVD, human disease, deaths
CARDIOVASCULAR DISEASE, CARDIOVASCULAR DISEASES, CVD, HYPERLIPIDEMIA, INTERACTION
These two authors contributed equally to this work.Prolonged postprandial hyperlipidemia may cause the development of cardiovascular diseases. This study explored whether postprandial triglyceride-rich lipoprotein (TRL) clearance responsiveness to Cardiovascular disease (CVD) is a leading cause of death globally, responsible for 32% of all-cause deaths, with 17.9 million people dying from CVD in 2019, according to the World Health Organization [There is compelling evidence to suggest that specific foods or components can positively impact the magnitude and duration of circulating TRLs, ultimately reducing the risk of CVD [The emergence of 16S rRNA sequencing and metagenomics techniques has provided novel opportunities to investigate the mutual interplay of gut microbiota, diet, and human disease [Therefore, we hypothesized in this study that the effect of PR on postprandial TRL clearance might be related to changes in the blood microbiome. To test this hypothesis, we conducted an 8-week randomized clinical trial (RCT) comparing PR to a placebo and evaluated postprandial TRL clearance and blood microbiota profiles at baseline and endpoint. In addition, the Qualitative Interaction Trees (QUINT) algorithm was utilized to differentiate between responders and non-responders based on pre-treatment characteristics. At the same time, linear discriminant analysis (LDA) coupled with effect size (LEfSe) was used to identify differentially abundant blood microbiota in responders versus non-responders. Finally, a machine learning algorithm was employed to elucidate the contribution of background blood microbiota to TRL clearance responsiveness.