Quantification of interleukins and antibodies
SARS-CoV-2 infection
BLOOD, SARS-COV-2 INFECTION
The assessment of the GA/18β drug’s efficacy against SARS-CoV-2 infection involved the examination of its influence on inflammatory and immune responses. Blood samples underwent centrifugation at 4°C and 4500 r.p.m. for 5 minutes to isolate plasma, which was promptly stored at -70°C ± 10°C until processing for the quantification of cytokines and antibodies against SARS‐CoV‐2 (IgM and IgG) at 0, 3, 5, 8, 11, 16 and 30 days.Serum levels of cytokines, namely IL-4, IL-2, IL-1β, TNF-α, IL-17A, IL-6, IL-10, IFN-γ, IL-12, IL-8, and TGF-β1, were quantified utilizing the Immunoassay Legendplex (Biolegend) Kit, adhering to the manufacturer’s guidelines. The concentration of interleukins was determined by establishing a standard curve with an R value > 0.95. Antibodies were detected using an ELISA test, following the method described by Stadlbauer et al. (