2.2.1. Biochemical and Anthropometric Examination
BLOOD
At the beginning of this study, the BMIs of all subjects were computed. InBody Body Composition Analyzers were used to measure body composition (InBody230, InBody Co., Ltd., Seoul, Republic of Korea). Body weight and other measurements of body composition, such as lean body mass, body fat mass, BMI, water content, and body fat percentage, were taken, with measurements of the subject’s weight being taken to the nearest 0.5 kg.Blood samples were taken before this study began and at its conclusion, eight weeks following the diet, for biochemical measurements. After centrifuging the serum, samples were kept in aliquots at 80 °C until additional analysis. The longest period of storage was 8 months.Biochemical parameters were estimated by spectrophotometric methods using the ARCHITECT c Systems device (Abbott Park, IL, USA) in the Department of Biochemistry of the Institute for Clinical and Experimental Medicine in Prague. Serum levels of myokines (brain-derived neurotrophic factor (BDNF), fatty acid binding protein 3 (FABP3), fractalkine, follistatin-like protein 1 (FSTL1), IL6, musclin, oncostatin M, osteonectin) were analyzed by the multiplex assay MILLIPLEX