Cell lines and Cell Culture
human breast cancer
MCF10A, the normal breast epithelial cell line, was obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in specific epithelial culture medium (CM-0525, Procell Co., Ltd). Furthermore, the human breast cancer cell line BT549 was purchased from Sailybio Co., Ltd. (Shanghai, China) and cultured in RPMI-1640 medium (meilunbio, China) supplemented with 10% fetal bovine serum (FBS; meilunbio, China) and 100 U/ml penicillin and streptomycin in a humidified atmosphere of 5% CO2 at 37 °C.