Materials and methods
tooth pics, gingivitis
MINOR, GINGIVITIS, PLAQUE
The experimental gingivitis model [The participants were bachelor- and master students at the Faculty of Dentistry, University of Oslo. These took special interest in the study and its carry-through since clinical research design is one of their subjects. Inclusion criteria comprised test persons 18 years or older, having at least three of the following teeth in contralateral quadrants; canine, premolars and 1Twenty-five individuals volunteered for the study. Prior to baseline, three withdrew for personal reasons resulting in 22 participants signing the informed consent form. The participants were given a minor economic compensation (100 EUR) for the inconvenience. All information, administration and data collection were performed at the Department of Periodontology, Institute of Clinical Dentistry, University of Oslo, Norway.At baseline, all participants received professional dental cleaning with rubber cup, pumice paste and dental floss, leaving the plaque score for every participant at zero. Individual fitted trays had been produced to fit the test- and control quadrants. Together with these, the participants received the test dentifrice (Pdx), and control, (Sensodyne Repair and Protect™, GlaxoSmithKlein, United Kingdom) and were instructed to apply the test dentifrice in one quadrant and the control dentifrice in the contralateral. Morning and evening, when the participants normally performed their oral hygiene routines, they first rinsed with water for 30 s. After placing the individual fitted tray, with its contents of test and control toothpaste, they upheld their regular oral hygiene regime in the opposing jaw where no registrations were done. After 1 min, the participants rinsed their mouth with water for 30 s. Then they removed the individual fitted tray and rinsed their mouth immediately with water for another 30 s. No dental cleaning, tooth pics or other, was allowed in these two, test and control, quadrants during the 21 days the experiment lasted. They repeated this procedure during the following 21 days. At day 7 and 14, the participants received a text message from the project manager with a short questionnaire about their adherence the protocol. All participants responded in kind, and no reminders had to be given. The use of individually fitted trays with their contents of test and control dentifrices secured that individual brushing technique, different biofilm qualities and abrasive qualities of the dentifrices were of no concern.At day 21, one researcher (HRP) personally interviewed the participants about their adherence to the protocol, as well as complaints and subjective side effects. Plaque—(PlI) and Gingival Index (GI) [