Effects of two-week clenbuterol treatment on glucose homeostasis
VASODILATION
Following two weeks of treatment (day 14), participants arrived at the research unit at 17:00 to first determine endothelial function and blood flow responses using flow-mediated vasodilation of the femoral artery in the leg. Afterwards (~18:00), participants received a standardized meal during which the last capsule of the respective study period was consumed. Participants then stayed overnight in a respiration chamber to determine sleeping metabolic rate and substrate oxidation. The following morning, body composition was determined by means of air displacement plethysmography (BodPod). Afterwards, blood pressure and heart rate were measured, and a muscle biopsy was taken in the overnight fasted state. Subsequently, a two-step hyperinsulinemic-euglycemic clamp with indirect calorimetry was performed for the determination of hepatic and peripheral insulin-stimulated glucose disposal.