Detection of protein expression in tumor tissues
HCC
MUTANT, EVENT
Immunohistochemical staining was used to detect the expression patterns of GPC3, CK19, vimentin, p53, AFP, EGFR, RRM1, BRCA1, VEGF, TS, Ki 67, ARG and GS in HCC tissues. The staining procedure was performed according to the instructions of the Roche benchmark XT Ventana (Roche Diagnostics, USA) automatic staining instrument, and the paraffin sections were detected by automatic immunohistochemistry. The slices were dewaxed, pretreated with cleaning buffer for 30 min, and then washed with reaction buffer. Subsequently, the following primary antibodies were added: GPC3, CK19, vimentin, mutant p53, AFP, EGFR, VEGF (all sourced from Santa Cruz Biotechnology, USA), RRM1, and BRCA1 (both sourced from Abcam, UK), After incubation for 30 min, the cells were stained with ultraView Universal DAB Kit (Roche Diagnostics, GER).Positive criteria: All immunohistochemical staining results were judged independently by two senior pathologists, and unanimous interpretations are made in the event of a disagreement. The agreement between the two experts on the positive results of immunohistochemical staining was 97.15% (2526/2600). Furthermore, 74 immunohistochemical stained sections with inconsistent judgments were agreed upon after consultation.