Biospecimen collection and processing
Tumour, tumour
TUMOUR, TUMOUR, ACD
Archival or fresh tumour biopsy specimens were formalin-fixed and paraffin-embedded (FFPE). Paraffin embedding was performed at the study site or at CellCarta. Tumour sectioning was performed by CellCarta, and cut sections were used for sequencing at Personalis or for fluorescence microscopy. Screening peripheral blood samples for sequencing were collected in K2EDTA tubes, processed at Precision for Medicine and sequenced at Personalis. Peripheral blood samples for T cell identification at screening or on-treatment analysis were collected in ACD or CPT tubes and shipped to Precision for Medicine for PBMC isolation and cryopreservation. For clinical cell product manufacture, a whole leukapheresis product was obtained from the patient at the study site and shipped overnight to the study sponsor. Samples from each infusion cell product and final clinical cell manufacture product were cryopreserved. Serum and plasma were collected and cryopreserved until analyses.