2.11. RNA extraction for the rRNA-targeted RT-qPCR
The RNA was extracted from the fecal samples by the Trizol method, using the RNAeasy kit (Qiagen, USA) and NucleoSpin RNA stool kit (Takara Bio Inc.) as per manufacturer guidelines. The extracted RNA was quantified, and quality was assessed by Tape station (Agilent Technologies Inc., USA) and Bioanalyzer (Agilent Technologies Inc., USA).