Functional contribution of the interventional product on the fecal microbiome
Shotgun metagenomics data was used to assess microbiome functional changes in response to multi-strain probiotics intake.Microbial functional alpha-diversity showed no change following multi-strain probiotics consumption (Functional alpha-diversity analysis expressed as values and as changes from baseline to the end of multi-strain probiotic intervention at species level. The Gene Set Enrichment Analysis (GSEA) was conducted to evaluate potential differential changes in function analysis between the KEGG groups in the hierarchical levels L1, L2 and L3 used by the KEGG classification (Although functional alpha-diversity did not change, gene set enrichment analysis showed statistically significant changes in certain functional gene abundance. These genes comprised functions involved in metabolism, genetic and environmental information processing and cellular processes.