2.3.3. Gut Microbiota Analysis
Microbial DNA was isolated from 250 mg of feces using a QIAamp PowerFecal Pro DNA Kit (QIAGEN, Hilden, Germany). The samples were sent to the Centre d’expertise et de Services Génome Québec (Génome Québec, Montréal, QC, Canada) for 16S rRNA sequencing. The V4 region of the 16S rRNA gene was amplified using primer 515F–806R, reverse-barcoded: GTGCCAGCMGCCGCGGTAA/GGACTACHVGGGTWTCTAAT, according to the manufacturer’s protocols. AmpliconSeq sequencing was performed on the NovaSeq platform (Génome Québec, Montréal, QC, Canada) (detail in 