White blood cell counting and leukocyte subtyping
LYSED
The total WBC count was determined in an aliquot of the EDTA blood using a Sysmex hematologic analyzer (Sysmex Austria, Vienna, Austria). For leukocyte subtyping red blood cells were lysed in another aliquot of the EDTA blood, and the remaining leukocytes were stained with fluorescently labelled antibodies against CD3, CD4, CD8, CD14, CD15, and CD19 (all from Thermo Fisher Scientific, Vienna, Austria). Sample acquisition was performed on a Gallios Flow Cytometer (Beckman Coulter, Indianapolis, IN, USA) and data was analyzed using the Kaluza 2.1 software (Beckman Coulter). See Supplementary Figure S