Concentrations of EVG, TAF and TFV in vaginal fluid
One participant in Group 2 was not included because her pre and post vaginal fluid sample weights were not provided to the PK central laboratory due to a temporary scale malfunction, thus preventing calculation of fluid volume. As shown in Concentrations of EVG, TAF and TFV (ng/mL) in vaginal fluid.*Means and Medians do not include BLQ values.TFV, TAF and EVG in Vaginal Fluid. Black = TFV concentrations in ng/mL; Red = TAF concentrations in ng/mL; Blue = EVG concentrations in ng/mL.Similar to EVG, the median vaginal fluid concentration of the prodrug TAF was highest at 4 hours post-dose (median 2,319,007 ng/mL), decreased by approximately 10-fold at 24 hours post-dose, and was markedly lower by 48 and 72 hours post-dose. By 7 days post-dose, 7 of 16 participants had BLQ TAF levels in vaginal fluid and median TAF levels were markedly lower relative to the 48- and 72-hour post dose measurements (Finally, median vaginal fluid concentration of TFV was highest at 4 hours post-dose (median 227,644 ng/mL), maintaining similar concentrations at 24 hours post-dose. By 48 hours post-dose, the median TFV levels in vaginal fluid were approximately 8-fold lower than the 24-hour post dosing concentrations, showing an additional slight (less than 2-fold) decrease by 72 hours post-dose. At 7 days post-dose, TFV levels in vaginal fluid remained detectable for all participants (