Gene and protein expression
There were no interactions between group and time for E3 ubiquitin ligases, Atrogin-1 (Atrogin-1 (A) and MuRF1 (B) gene expression, and the ratio of phosphorylated to total protein of mTOR (C), p70S6K (D), S6K (E), and 4EPB1 at pre- (day 14) and post- (day 28) immobilization. Representative western blot bands (G). Boxes represent the 25