Exosome isolation from ProtheraCytes
ProtheraCytes were seeded in a serum-free medium (DMEM/F12 medium without phenol red, Thermo Fisher) in a bioreactor for extracellular vesicle release by exerting a controlled mechanical stimulation on cells for 30 min to 2 h according to Everzom’s proprietary methodFor miRNA analysis, ProtheraCytes were cultured at the concentration of 2.5 × 10