2.5. Lipidomic Pattern Analysis
The lipid separations were carried out on an AcquityTM UPLCThe MS analysis was performed with ESI-MS/MS operating in positive ionization mode. The next parameters were established: source temperature: 120 °C; nebulization NNormalization factors were calculated for each lipid (in each sample) by dividing its intensity by the recorded intensity of an appropriate internal standard in the same sample. Any remaining sample injection variable response null values in the corrected data set were replaced with missing values before averaging to form the final data set used for statistical analyses of the study samples [Lipid classification was carried out according to the all-encompassing categorization system put forth by Fahy et al. [