3.3. Gut Microbiota Analysis
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Sequencing the 16S rRNA gene from stool samples generated 1,383,378 raw sequences. After filtering and cleaning the sequences, 1,001,142 good-quality sequences were obtained. The Good’s coverage obtained in samples was >99%, indicating good sequencing coverage. Raw filtered reads and normalized read counts per group are shown in The α diversity, microbial richness, and diversity were not different for the Chao1 (Intervention groups presented 21 phyla, 35 classes, 77 orders, 134 families, and 302 genera. All groups had eight predominant phylum: Firmicutes (sorghum group: 61.45 ± 4.92%; wheat group: 62.81 ± 7.55%), followed by Bacteroidetes (sorghum group: 29.35 ± 4.67%; wheat group: 26.17 ± 6.92%), Proteobacteria (sorghum group: 3.87 ± 1.09%; wheat group: 4.34 ± 2.18%), Actinobacteria (sorghum group: 2.75 ± 1.18%; wheat group: 3.10 ± 1.02%), and Desulfobacterium (sorghum group: 1.04 ± 0.47%; wheat group: 1.06 ± 0.42%) (