Statistical methods
This study was performed to compare the qualitative and quantitative effects of two remineralizing agents on post-orthodontic white spot lesions comprising different theories. The first remineralizing agent is the self-assembling peptide P11-4 which follows the non-classical theory of remineralization while the second one is the fluoride varnish material that follows the classical theory for the remineralization.The mean and standard deviation values were calculated for each group in each test. Data were explored for normality using the Kolmogorov–Smirnov and Shapiro–Wilk tests, remineralizing process data showed parametric (normal) distribution, while ICDAS data showed non-parametric (not-normal) distribution.An independent sample The Mann–Whitney test was used to evaluate two groups in unrelated samples using non-parametric data. To compare more than two groups in related samples, the Friedman test was utilized. To compare two groups in related samples, the Wilcoxon test was utilized. The significance level was set at 