2.3. Blood collection and indicator measurements
TG
Fasting blood samples (5 mL anticoagulant and 5 mL non-anticoagulant) were collected from 8:00 to 9: 00 on the day at the end of each intervention. IL-6, TNF-α, CRP, FIB, CD62P, CD40L, ICAM-1, NO, ET-1, MMP-2 and MMP-9 levels were tested by a professional testing institution. CHO, TG, HDL-C and LDL-C levels were tested by the laboratory of the hospital. IL-6, TNF-α, CD62P, CD40L and ICAM-1 was detected by a bead-based multiple flow cytometry on Luminex 200 system (Luminex Corporation, Austin, TX, USA) with chips (IL-6 and TNF-α: Merck Millipore, Germany; CD62P, CD40L and ICAM-1: R&D, USA). CRP, NO, MMP-2, MMP-9, FIB and ET-1 was separately detected by ELISA kit (CRP, NO, MMP-2, MMP-9: R&D, USA; FIB: NOVUS, USA; ET-1: Elabscience Biotechnology Co. Ltd, Wuhan, China).In addition, a compound index-atherogenic index of plasma (AIP) was calculated by the results of TG and HDL-C levels and the formula: 