Pharmacokinetic assessments
BLOOD, SEPARATION
Blood samples for pharmacokinetics analyses were collected predose on days 1, 7, 14, 21, and 28, and 30 min, 1, 2, 4, 6, 10, and 12 h postdose on days 1 and 28. The concentrations of unecritinib and unecritinib M were measured by tandem mass spectrometry hyphenated to liquid chromatography separation systems (HPLC–MS/MS). The pharmacokinetics parameters were calculated by non-compartmental analysis (NCA) model using WinNonLin