Outcomes
Listeria
SECONDARY, DEGENERATE, HAEMOPHILUS
The primary outcome of the study is related to the formation of the embryos, both
on the 5The embryonic classification of Group D5 was performed according to the degree of
blastocoel expansion (1 to 4), 1 - early blastocyst, 2 - blastocyst, 3 -
expanded blastocyst and 4 - hatched blastocyst (To calculate the cleavage rate, the number of degenerate oocytes found after
culture was excluded.The secondary outcome is related to the quality of the culture medium recovered
from the INVOCell™ device. The pH and microbiological analysis were the
parameters evaluated to prove the safety and efficacy of the device in keeping
the culture medium balanced.The pH measurement was performed in both groups immediately after the recovery of
the embryos from the INVOCell™ device, and the IVF plate using a pH meter
device (OHAUS starter3100). The ideal pH value of the culture medium was between
7.26 and 7.33. A 100 µl aliquot of the culture media of the participants
in Group D5 were subjected to analysis by Total Automation WalkAway
(Dade-Behring), identifying fermenting and non-fermenting Gram-Negative
bacteria, Gram-Positive Cocci and Listeria, Anaerobic, Yeasts and fastidious
microorganisms such as Neisseria and Haemophilus.