Body composition
acromion, hormonal-induced fluctuations, Rosenfeld et al.
Body composition was measured seven days after menstruation in both the pre- and post-intervention periods to avoid potential confounding issues due to hormonal-induced fluctuations in extracellular water (Rosenfeld et al. Each subject was scanned by a certified technician, and computer algorithms distinguished bone and soft tissue, edge detection, and regional demarcations (software version APEX 5.6.0.7, Hologic Horizon A, Bedford, MA). For each scan, subjects wore sport clothes and were asked to remove all materials that could attenuate the X-ray beam (e.g., jewelry items, underwear containing wire, etc.). Calibration of the densitometer was checked daily against a standard calibration block supplied by the manufacturer. The abdominal region was delineated by an upper horizontal border located at half of the distance between acromion and external border of the iliac crests, a lower border determined by the external end of iliac crests and the lateral borders extending to the edge of the abdominal soft tissue. All trunk tissue within this standardized region was selected for analysis. To determine intertester reliability, two different observers manually selected the area for each subject with a coefficient of variation = 0.263%.