Quantification of intracellular glucose
To detect glucose uptake, we used 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)–2-Deoxyglucose (2-NDBG) a fluorescent analog of glucose (Invitrogen, USA; N13195). 2-NDBG is transported through SGLT-1 and GLUT-2. Increased uptake leads to 2-NDBG accumulation in the cells. Cells infected with SARS-CoV-2 for 96 hr were exposed to 6 mM of 2-NDBG for 24 hr. Cells were then fixed, counterstained with 1 μg/mL Hoechst 33258. Staining intensity was normalized to Hoechst 33258 across multiple fields of view.