Lipid layer thickness (in ICU) at baseline and consecutive measurements.
The measurement values up to 120 min after application were compared with the baseline using the Wilcoxon test. All p-values were adjusted for 3 comparisons according to the Bonferroni method. An asterisk with the corresponding p-value indicates a significant change compared to baseline measurement. ICU = Interferometric Colour Units; CN = cationic nanoemulsion; F6H8 = perfluorohexyloctane.At baseline, there was no difference of RMS values between the three groups (H (2) = 1.2; p = 0.5). Immediately after application, CN increased RMS (z = -2.6; p = 0.027) from 0.42 ± 0.06 μm to 0.48 ± 0.08 μm (