2.5. Fasting Serum Lipid Profile & Biomarkers
inflammation
INFLAMMATION
The fasting serum lipid profile, adipokines, and inflammatory markers were measured in fasting plasma by the DORI Metabolic Core Laboratory. The fasting serum lipid profile included triglycerides, total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, and the total cholesterol:HDL cholesterol ratio. Serum lipids were assayed using FujiFilm kits utilizing the microplate method (FUJIFILM Wako Diagnostics, Mountain View, CA, USA). The intra- and inter-assay coefficient of variation (CV) for the HDL-Cholesterol E assay is 8.23% and 3.71%, respectively; the intra- and inter-CV for the Cholesterol E assay is 8.80% and 7.75%, respectively; and the intra- and inter-assay CV for the L-Type Triglyceride M assay is 8.23% and 3.71%, respectively. Adipokines, including Monocyte Chemoattractant Protein-1 (MCP-1) and leptin, as well as markers of low-grade chronic systemic inflammation as assessed by concentrations of interleukin-6 (IL-6), and TNF-Alpha were measured in fasting plasma using the Millipore Magpix Metabolic Panel kit at the DORI Core Laboratory (intra-assay and inter-assay CV of 4.53% and 6.96%, respectively). High-sensitivity C-reactive protein (CRP) was assayed separately using the Millipore ELISA kits, with an intra- and inter-assay CV of 4.60% and 6.00%, respectively.