In vivo hydroxychloroquine significantly suppressed IL-6 secretion after TLR7 stimulation, but not following TLR3, TLR9, or RIG-I-like receptor stimulation
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Activation of NFκB signaling via endosomal TLR and RIG-I-like ligands was assessed by measuring downstream IL-6 production (Fig. In vivo HCQ inhibited IMQ-induced IL-6 release, but not TLR3, TLR9, and RIG-I. PBMCs were stimulated with 50 μg/mL poly I:C (TLR3), 1 μg/mL IMQ (TLR7), 2.5 μM CpG A (TLR9), or 1 μg/mL poly I:C/lyovec (RIG-I) at 0, 12, 24, 48, 72, and 92 h after primary HCQ dosing. IFNα release was measured by ELISA. Data is shown as mean + SD as one-sided error bars. Dotted vertical lines indicate HCQ dosing times