2.4. Statistical Analysis
The sample size of fifty subjects was calculated using the superiority design method (calculating sample size when the outcome measure is a continuous variable) [Statistical analysis was performed using SPSS software version 22 (SPSS Inc., Chicago, IL, USA). Data were ensured using the normal distribution of variables by the Kolmogorov–Smirnov test, and variables that were non-normally distributed were log-transformed before statistical analysis.The descriptive analysis was expressed as an absolute number and percentage. Continuous variables were expressed using means and standard deviations (SDs) and associated two-tailed 95% confidence intervals (CIs). We applied the means substitution method to handle missing values. The sensitivity analysis in which the missing values were not imputed was performed. Outcomes were compared to the baseline during a study period, and a paired 