Limitations
MINOR
Despite the dietary counselling and adaptations made to dietary energy content in case volunteers lost weight in the first week, body weight was reduced by, on average, ~0.8–1.2 kg in both diets. However, body weight was reduced to a similar extent in both diets. The small difference of 0.4 kg between the interventions is expected to lead to a very minor decrease in liver fat of <0.3%, which is much smaller than the differences found in the present study [In the present study, a clear distinction in the glucose pattern could especially be made upon lunch and also after dinner as measured by CGM. The specific menus used on day 14 of the interventions clearly differed in GI for lunch (33 vs. 57), dinner (43 vs. 63), and after-dinner snack (33 vs. 64). The GI of the breakfast meals provided to the volunteers on day 14 were, however, less distinctive (45 vs. 52), which could explain why the expected differences in glycemic response are not seen after breakfast. We recognize that the sample size in this study was limited, which could potentially explain the lack of findings with respect to exploratory outcomes such as de novo lipogenesis and liver glycogen content. The analysis of specific liver fat composition requires high-quality spectra to allow for the calculation of percentage PUFA and MUFA. In the current study, several measurements of IHL composition had to be excluded due to a low signal-to-noise ratio, especially in volunteers with very low liver fat content. In populations with higher liver fat content, a bigger signal is available, thus leading to better quality. 