Biochemical assessment
Data were collected in the morning, after a 12–14 h overnight fasting at the beginning and the end of the trial. Venous blood samples (10 mL) were drawn into tubes containing EDTA or heparin, then immediately centrifuged (Hettich D‐78532) at 3000 r.p.m. for 10 min to separate serum and stored at −80°C until analysis. Serum levels of myogenin, myostatin, and IGF‐1 were determined by enzymatic methods using ELISA kits (Cusabio Ulm) and creatine kinase by auto‐analyzer system (Selectra E, Vitalab). Moreover, Serum LC was measured by an ELISA kit (Cusabio Ulm) and ELISA plate reader (Elx800, Bio‐Tek Instrument Inc.) at a wavelength of 450 nm.