Automated method of PRP preparation
STERILE
After the evaluation of patients’ blood vessels and build‐up of venous access, the automated blood cell separator (Fresenius COM.TEC, Germany) and the Plt‐5d (Platelet collection) procedure were used to prepare 30 ml of PRP by professionals of the transfusion department. PRP was divided into three sterile tubes (NEST 15 ml centrifuge tube) on average, frozen and stored at ‐40°C. PRP samples prepared by automated blood cell separator (automated method) were analyzed by automatic blood cell analyzer (BC‐7500). Platelet count in PRP was determined and compared with the whole blood samples of each patient (Table Platelet concentration in whole blood and PRP (prepared by automated method).