2.6. Blood Collection
BLOOD, FRANKLIN
Blood samples for hormone analysis were collected in lithium–heparin-coated tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) for motilin analysis and ethylenediaminetetraacetic acid (EDTA)-coated tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) for ghrelin and insulin analysis. Blood was supplemented with 500 kIU aprotinin/mL of blood. Blood collected for ghrelin measurements was additionally supplemented with 0.57 mM of phenylmethylsulfonyl fluoride solution (Sigma-Aldrich, Steinheim, Germany). Samples were centrifuged at 4 °C for 10 min at 1400× 