3.5. Sequencing Quality and Taxonomic Composition of Gut Microbiota
After filtering the low-quality reads, chimera removal, and denoising, a total of 18,722,472 reads was acquired from 110 samples, with an average of 170,204 reads per sample. More than 97% of the sequencing reads from each sample displayed a Phred quality score of 20. The composition and diversity of gut microbiota of participants before and after intervention were assessed. Bacteria with top 5 and 16 relative abundances at the phylum (