MN 310 travels north through the city as 5th Avenue NW. It passes just west of the Roseau County Fairgrounds before leaving the city and continuing north through rural northern Minnesota. A few miles north of Roseau, the route crosses the Roseau River, then bends slightly to the east to avoid crossing it twice.
North of the Roseau River, the highway crosses the Sprague Creek before entering the Lost River State Forest. MN 310 treks through the forest for a few miles, then curves to the northeast just south of the Canadian border. The route then reaches the border and continues into Manitoba as PR 310.