The regiment was originally formed as a part of the 135th Aviation Regiment with Regimental Headquarters located in Frankfort, Kentucky, 1st Battalion in Missouri and 2nd Battalion as a part of the Colorado Army National Guard as 2nd Battalion 135th Aviation, under the United States Army Regimental System.
The regiment was constituted in August 1985 with the activation of the 35th Infantry Division as an Attack Battalion flying AH-1S Cobra's.