(2011) developed a much more efficient method for the production of RNAi vectors in which expression of the RNAi can be knocked-in using Cre-recombinase and knocked-out using Flp-recombinase. The novel GRIM Expression System allows for the much faster generation of expression vectors containing artificial RNAi constructs. The authors went on to show that their expression system works quite effectively in human embryonic kidneys (HEK) cells, a common human immortalized cell line in molecular research.
In 2011, researchers developed a new method for producing RNAi vectors that allows for the controlled expression and silencing of genes using Cre-recombinase and Flp-recombinase. This novel GRIM Expression System significantly accelerates the creation of RNAi constructs and has been successfully tested in human embryonic kidney (HEK) cells, a common cell line used in molecular research.