They have diploid chromosome numbers of 30, 32, 34, and 52. Males produce the most sperm in autumn and early winter, suggesting that mating typically occurs around that time, and pregnant females have been caught between February and April. Historically, evidence of hybridization between the Yucatan yellow bat and the Black-winged little yellow bat has been observed.
The species exhibit a diploid chromosome number of 30, 32, 34, and 52. Males produce the most sperm in autumn and early winter, indicating that mating likely occurs during this period. Pregnant females have been captured between February and April.  Hybridization between the Yucatan yellow bat and the Black-winged little yellow bat has been documented historically.