Although Hormone-Sensitive Lipase (HSL) is able to catalyze hydrolysis of triglycerides and diglycerides, another enzyme found in adipose tissue, Adipose triglyceride lipase (ATGL), has a higher affinity for triglycerides than HSL, and ATGL predominantly acts as the enzyme for triglyceride-specific hydrolysis in the adipocyte. Hormone-sensitive lipase, which has 11-fold greater affinity for diglycerides than triglycerides, predominantly cleaves these diglycerides, forming 2-monoglyceride and a free fatty acid.
Hormone-Sensitive Lipase (HSL) can hydrolyze both triglycerides and diglycerides, but Adipose Triglyceride Lipase (ATGL), an enzyme found in adipose tissue, has a stronger affinity for triglycerides. ATGL primarily catalyzes triglyceride-specific hydrolysis within adipocytes. In contrast, Hormone-Sensitive Lipase (HSL) predominantly cleaves diglycerides into 2-monoglyceride and a free fatty acid.