Fisherman Island National Wildlife Refuge is located near the United States Fish and Wildlife Service's Eastern Shore of Virginia National Wildlife Refuge, and is cut in half by the presence of U.S. Highway 13 and the Chesapeake Bay Bridge Tunnel.
The location of Fisherman Island National Wildlife Refuge is adjacent to the United States Fish and Wildlife Service's Eastern Shore of Virginia National Wildlife Refuge, with both areas separated by U.S. Highway 13 and the Chesapeake Bay Bridge Tunnel.