After becoming regent, Laura brought into her council ministers who had proven effective and devoted to the House of Este. Count Girolamo Graziani, jurist Bartolomeo Gatti, Cardinal Rinaldo d'Este, Cesare Ignazio d'Este, Marquess of Montecchio, and Laura's confessor, the Jesuit Andrea Garimberti, helped her rule the duchy.
Upon taking office as regent, Laura selected ministers who had shown their worth and commitment to the Este family. Count Girolamo Graziani, jurist Bartolomeo Gatti, Cardinal Rinaldo d'Este, Cesare Ignazio d'Este, Marquess of Montecchio, and her spiritual guide, Jesuit Andrea Garimberti, worked alongside her in administering the duchy.