Such differences are likely due to pH changes or differences in primary structure of the luciferase. Modification of the firefly luciferin substrate has led to "red-shifted" emissions (up to emission wavelength of 675 nm).
Such variations are probably caused by pH changes or differences in primary structure of luciferase. Alterations to the firefly luciferin substrate have resulted in "red-shifted" emissions (up to an emission wavelength of 675 nm).