The FIE3 lies within a 201-nucleotide sequence just upstream of the polyadenylation signal and can act autonomously to destabilize a heterologous mRNA. Further deletion constructs identified an essential 68-nucleotide element within the FIE3.
The FIE3 is situated in a specific 201-basepair region preceding the polyadenylation marker and has the ability to function autonomously to disrupt the stability of a foreign mRNA, highlighting the importance of its 68-basepair core element.