The NBD peptide consists of the region from T735 to E745 of the IKK-β subunit fused with a sequence derived from the Antennapedia homeodomain that mediates membrane translocation. Furthermore, wild type NBD peptide has been shown to dose-dependently inhibit interaction of IKKB with NEMO compared to mutant controls. Additionally, NF-κB activation was suppressed in HeLa cells after incubation with NBD wild type peptides.
The NBD peptide comprises the segment from T735 to E745 of the IKK-β subunit combined with a sequence derived from the Antennapedia homeodomain responsible for membrane translocation. Additionally, wild-type NBD peptides have been demonstrated to dose-dependently inhibit the interaction between IKKB and NEMO compared to mutant controls. Furthermore, NF-κB activation has been suppressed in HeLa cells following incubation with wild-type NBD peptides.