Additionally, RNA polymerase contains a core Mg+ ion that assists the enzyme with its catalytic properties. RNA polymerase works by catalyzing the nucleophilic attack of 3’ OH of RNA to the alpha phosphate of a complementary NTP molecule to create a growing strand of RNA from the template strand of DNA. Furthermore, RNA polymerase also displays exonuclease activities, meaning that if improper base pairing is detected, it can cut out the incorrect bases and replace them with the proper, correct one.
Moreover, RNA polymerase contains an essential Mg+ ion that supports its catalytic capabilities. It catalyzes the nucleophilic attack of 3’ OH on an RNA molecule by a complementary NTP to form a new RNA strand from a DNA template. Additionally, RNA polymerase demonstrates exonuclease activities, allowing it to remove and replace incorrect bases if there is improper base pairing.