This exchange is utilized to separate proteins that have faintly differing charges. Like all anion exchangers, the resin carries a positive charge that interacts favorably with negative charges. The positive charge of DEAE cellulose is due to a protonated amine group.
The process is used to differentiate proteins based on their subtle charge differences. Similar to other anion exchangers, the resin carries a positive charge that effectively interacts with negative charges. The positive charge in DEAE cellulose stems from a protonated amine group.