The route passes to the east of Prestonburg Community College and narrows to a two-lane unnamed road, curving northeast through woods and running to the east of Levisa Fork. KY 321 passes more rural development and intersects the western terminus of KY 3024.
The route runs east of Prestonburg Community College, then becomes a two-lane road that curves northeast through wooded areas before continuing east of Levisa Fork. As it passes through more rural landscapes, KY 321 meets at the western endpoint of KY 3024.