The Buffalo Southern Railroad operates the portion from Buffalo to the Erie County line at Gowanda, and the New York and Lake Erie Railroad operates the line south from Gowanda to South Dayton, including an excursion train that runs in the summer.
The Buffalo Southern Railroad is responsible for the segment between Buffalo and the Erie County border at Gowanda, whereas the New York and Lake Erie Railroad handles the southern stretch of track from Gowanda to South Dayton, featuring an excursion service that operates seasonally.