The colour is due to the hydroxyl group (-OH) of cholesterol reacting with the reagents and increasing the conjugation of the un-saturation in the adjacent fused ring. Since this test uses acetic anhydride and sulfuric acid as reagents, caution must be exercised so as not to receive severe burns.
The color is due to the interaction between the hydroxyl group (-OH) of cholesterol and reagents, which increases conjugation in the adjacent fused ring. Given that this test employs acetic anhydride and sulfuric acid as reagents, it's important to be cautious to avoid severe burns.