In suspension-cultured maize cells, dimerisation of ferulic acid esterified to polysaccharides occurs mostly in the protoplasm, but may occur in the cell walls when peroxide levels increase due to pathogenesis. In suspension-cultured wheat cells, only the 8,5'-diferulic acid is formed intraprotoplasmically with the other dimers being formed in the cell wall.
In plant cells grown in a liquid environment, ferulic acid molecules linked with polysaccharides primarily occur inside the cell's interior space, but can also happen in the cell walls when oxygen-containing compounds increase due to disease. In wheat cells of the same type, only one specific dimer is produced within the cytoplasm, while others are created in the cell wall.