Missense mutations of MEN1 are especially important as they result in a change to crucial amino acids needed in order to bind and interact with other proteins and molecules. As menin is located predominantly in the nucleus, these mutations can impact the stability of the cell and may further affect functional activity or expression levels of the protein.
Mutations in MEN1 that result in missense changes are particularly critical since they affect essential amino acids required for protein-protein interactions. Given menin's predominant location in the nucleus, such mutations can compromise cellular integrity and potentially alter protein activity or expression.