It forms the U5 snRNP (small nuclear ribonucleoprotein) by associating with several proteins including Prp8 - the largest and most conserved protein in the spliceosome, Brr2 - a helicase required for spliceosome activation, Snu114, and the 7 Sm proteins. U5 snRNA forms a coaxially-stacked series of helices that project into the active site of the spliceosome.
It constitutes the U5 snRNP (small nuclear ribonucleoprotein) by linking with several proteins including Prp8 - the largest and most preserved protein in the spliceosome, Brr2 - a helicase necessary for spliceosome activation, Snu114, and the 7 Sm proteins. U5 snRNA forms a coaxially-stacked series of helices that protrude into the active site of the spliceosome.