When attempting to identify biological molecules that would disrupt the formation of the F complex, researchers developed a high-throughput fluorescence polarization (FP)-binding assay. In this assay, a small peptide of a known sequence was synthesized and tagged with a fluorescent molecule.
In an effort to identify molecules that would disrupt the assembly of the F complex, researchers designed a high-throughput assay utilizing fluorescence polarization. A short peptide with a known sequence was created and tagged with a fluorescent marker for detection.