NY 65 passes into Livingston County in the town of Lima upon crossing over Honeoye Creek in the center of the community; however, it remains in the county for just  before following Honeoye Creek into Monroe County and the village of Honeoye Falls in the town of Mendon situated on the county's southern edge.
NY 65 begins its journey in the town of Lima, Livingston County, after crossing over Honeoye Creek. It remains within the county until it enters Monroe County and flows into the village of Honeoye Falls in the town of Mendon, which is situated on the southern edge of the county.