It was found that one compound molecule binds to a single enzyme molecule. It was shown that 1-deoxynojirimycin (DNJ) would bind the strongest of the sugars tested and blocked the active site of the enzyme almost entirely. The studies enhanced knowledge of the mechanism by which α-glucosidase binds to imino sugars.
One molecule binds to one enzyme, 1-deoxynojirimycin (DNJ) was found to bind most strongly among tested sugars and blocked the active site of the enzyme almost completely. The studies advanced understanding of how α-glucosidase interacts with imino sugars.