Soon thereafter it was shown that phosphate-linked oligomers of the analogues do in fact exhibit hypochromicity in the presence of RNA and DNA in solution (Seita et al. 1972). The preparation of the polymers was later described by Cook et al.
In subsequent research, it became apparent that phosphate-linked oligomer analogues exhibit a decrease in color intensity when combined with RNA and DNA in a liquid environment (Seita et al. 1972). Later studies provided more detail on the synthesis of these polymer compounds by Cook et al.