Barbiturase acts as a catalyst in the second step of oxidative pyrimidine degradation, promoting the ring-opening hydrolysis of barbituric acid to ureidomalonic acid. Although grouped into the naturally existing amidohydrolases, it demonstrates more homology with cyanuric acid amidohydrolase.
In the context of oxidative pyrimidine degradation, barbiturase serves as a catalyst in the second step, enabling the conversion of barbituric acid into ureidomalonic acid through hydrolysis. Its enzymatic characteristics are more similar to those of cyanuric acid amidohydrolase.