Mitochondrial acetoacetyl-CoA thiolase, also known as thiolase II, the enzyme responsible for catalyzing the synthesis of acetoacetyl-CoA within ketogenesis as mentioned, is also involved within acetoacetyl-CoA cleavage in ketolysis. It is observed to play a role within cleavage of acetyl-CoA from acetoacetyl-CoA and 2-methylacetoacetyl-CoA.
In the context of ketogenesis, mitochondrial acetoacetyl-CoA thiolase II is essential for generating acetoacetyl-CoA. Furthermore, it plays a role in the degradation of acetyl-CoA from acetoacetyl-CoA and 2-methylacetoacetyl-CoA.