From their research the "Aschheim-Zondek test" for pregnancy was created, which involved injection of a patient's urine into an immature laboratory mouse. If the rodent displayed an estrous reaction, it represented a positive indication of pregnancy.
Researchers at Aschheim and Zondek created a pregnancy test based on their findings, involving the injection of a patient's urine into a young laboratory mouse. A positive response from the mouse, characterized by an estrous reaction, confirmed pregnancy.