Instead, the active site is pre-formed and glmS ribozyme activity is triggered by the introduction of a functional group on GlcN6P that is necessary for catalysis. As mentioned above, the amine group on GlcN6P is thought to be the catalytically involved functional group, which is supported by studies that evaluated glmS ribozyme activity using different ligands, under physiologic conditions.
The glmS ribozyme's activity is initiated when a functional group is attached to GlcN6P, which is essential for catalysis. This is consistent with previous explanations, where it was noted that the amine group on GlcN6P plays a key role in the catalytic process, as demonstrated by experiments using different ligands under normal physiological conditions.