Heteroduplex analysis (HDA) is a method in biochemistry used to detect point mutations in DNA (Deoxyribonucleic acid) since 1992. Heteroduplexes are dsDNA molecules that have one or more mismatched pairs, on the other hand homoduplexes are dsDNA which are perfectly paired. This method of analysis depend up on the fact that heteroduplexes shows reduced mobility relative to the homoduplex DNA.
Heteroduplex analysis (HDA) is a biochemical approach used to detect point mutations in DNA (Deoxyribonucleic acid) starting from 1992. Heteroduplex molecules are dsDNA with mismatched pairs, whereas homoduplexes have perfectly paired dsDNA. This method works on the principle that heteroduplexes move more slowly than homoduplex DNA.