In the United States military, the phrase is the motto of the US Army's oldest infantry regiment, the 3rd U.S. Infantry Regiment (The Old Guard), located at Fort Myer, Virginia. "Don't tread on me" is also used in the First Navy Jack of United States Navy.
The phrase "Don't tread on me" is also adopted by the United States Navy's First Navy Jack. It is also the motto of the oldest infantry regiment in the US Army, known as the 3rd U.S. Infantry Regiment (The Old Guard).