ADF/cofilin is found in ruffling membranes and at the leading edge of mobile cells. In particular, ADF/cofilin promotes disassembly of the filament at the rear of the brush in Xenopus laevis lamellipodia, a protrusion from fibroblast cells characterized by actin networks.
The role of ADF/cofilin is to break down actin filaments at the back of lamellipodia, which are projections from fibroblast cells that are made up of actin networks.