In 1856 he founded an ophthalmology hospital in Wiesbaden, and was its director until his death in 1879. At Wiesbaden, he worked closely with his brother, Hermann Pagenstecher (1844–1932), who took control of the eye hospital following his death.
In 1856, he established an ophthalmology hospital in Wiesbaden and served as its director until his death in 1879. At Wiesbaden, he collaborated closely with his brother, Hermann Pagenstecher (born in 1844 and died in 1932), who took charge of the eye hospital after his passing.