The pKa declines approximately 0.03 units per degree Celsius rise in temperature. This can lead to relatively dramatic pH shifts when there are shifts in solution temperature. Sodium chloride concentration may vary from 100 to 200 mM, tris concentration from 5 to 100 mM and pH from 7.2 to 8.0.
The pKa decreases by approximately 0.03 units for every degree Celsius increase in temperature. This can cause substantial pH fluctuations when solution temperatures change. Sodium chloride concentrations vary between 100 and 200 mM, tris concentrations range from 5 to 100 mM, and pH levels are typically within the range of 7.2 to 8.0.