Surprisingly, they isolated a very large multi-sub-unit supra-molecular complex, instead of a small protein. A combination of biochemical, biophysical, immun-ochemical and ultra-structural techniques, followed by molecular biological verification, led to the definition and proof of the cellulosome concept. The birth of the discrete, multi-enzyme cellulosome complex was thus documented.
In an unexpected finding, researchers discovered a large, multi-component supra-molecular structure, rather than a small protein. Through the use of various biochemical, biophysical, and immunological techniques, combined with molecular biological verification, the concept of the cellulosome was defined and proven. The emergence of the cellulosome complex as a distinct, multi-enzyme entity was thus recorded in the context of understanding cellular biology.