CNO activates the excitatory Gq- coupled DREADDs: hM3Dq, hM1Dq and hM5Dq and also the inhibitory hM4Di and hM2Di Gi-coupled DREADDs.
In the context of neuronal activation, CNO triggers the activation of a diverse set of DREADDs, including those that stimulate (Gq-coupled) or inhibit (Gi-coupled) neural activity, such as hM3Dq, hM1Dq, hM5Dq, hM4Di, and hM2Di.