The first half of the 16th century spelled problems in terms of religious service, and it is despite the Medininkai-Bystritsa Augustians receiving new endowments and donations. The discipline within the monastery deteriorated; in 1523 the Canons left Bystritsa, and in 1528 they were removed from Medininkai for "non-compliance with rules of the order"; it was manifested e.g. by drunken episodes and dereliction of duty.
The first half of the 16th century presented challenges in terms of religious service, and despite the Medininkai-Bystritsa Augustians receiving new resources and donations, their discipline within the monastery declined. In 1523, the Canons left Bystritsa, and in 1528 they were removed from Medininkai for "non-compliance with the order's rules"; this was evident through instances of excessive drinking and neglect of duties.