After delivery, Galileo Galilei together with other submarines of Archimede-class was assigned to 12th Squadron of the III Flotilla, later to become 41st and then 44th Submarine Squadron of the VII Submarine Group, based at Taranto. After a period of intensive training and short cruises, the Squadron was sent for training to Tobruk.
After delivery, Galileo Galilei, along with other submarines of the Archimede class, joined the 12th Squadron of the III Flotilla, which later became the 41st and then 44th Submarine Squadron of the VII Submarine Group stationed at Taranto. After a period of training and brief voyages, the squadron was dispatched to Tobruk for further instruction.