Born in Maubeuge in 1601, Desubleo probably learned his trade in Flanders, although there is no proof he was trained in the workshop of Abraham Janssens together with his stepbrother Nicolas Régnier. With the latter he moved to Rome, where he is recorded in 1624 and 1625. By the beginning of the 1630s, he was working in Bologna, in the busy workshop of Guido Reni, who was a crucial influence on him and on other artists of his age or slightly younger, including Simone Cantarini and Jean Boulanger.
Born in Maubeuge in 1601, Desubleo likely acquired his profession in Flanders, although there is no evidence he was trained in the workshop of Abraham Janssens alongside his stepbrother Nicolas Régnier. Along with the latter, he relocated to Rome, where he is documented in 1624 and 1625. By the early 1630s, he was working in Bologna, in the bustling workshop of Guido Reni, who had a significant impact on him and other artists of his era or slightly younger, including Simone Cantarini and Jean Boulanger.