In addition, there are a number of genes which contain multiple promoter and enhancer regions each regulated by a different HNF. Furthermore, efficient expression of these genes require synergistic activation by multiple HNFs. Hence hepatocyte nuclear factors function to ensure liver specific expression of certain genes.
Furthermore, there are several genes that have multiple regulatory elements, each controlled by a different HNF. For these genes to be expressed effectively, they require simultaneous stimulation from multiple HNFs. As a result, hepatocyte nuclear factors play a vital role in ensuring the liver-specific expression of certain genes.