Beginning from an interchange with the Big 4 Terminal Railroad line in Beesons, it runs north through Fayette County, then through the Wayne County communities of Milton and Cambridge City, then northwest into Henry County through New Lisbon to New Castle, where it joins a Norfolk Southern line.
The route begins at an intersection with the Big 4 Terminal Railroad near Beesons, heading north through Fayette County. It continues through Wayne County's communities of Milton and Cambridge City before moving northwest into Henry County via New Lisbon, ultimately joining a Norfolk Southern line in New Castle.