Anzolin is most noted for having played most of the 1960s with Italian Serie A giants Juventus, making 305 appearances (230 of which were in the league) with the club between 1961 and 1970. During his time at the club, he won a Serie A championship during the 1966–67 season, as well as a Coppa Italia during the 1964–65 season.
Anzolin is most notable for his prominent association with Juventus in the 1960s, where he made 305 appearances (230 of which were league matches) between 1961 and 1970. His contributions to the club's success during this period are highlighted by his wins in a Serie A championship in 1967 and a Coppa Italia in 1965.