The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose. Once the fusion protein is obtained in purified form, the protein of interest is often cleaved from MBP with a specific protease and can then be separated from MBP by affinity chromatography.
The fusion protein adheres to amylose columns while other proteins pass through. The MBP-protein fusion can be purified by eluting the column using maltose. After obtaining the fusion protein in its pure form, the target protein is typically removed from MBP with a specific protease and then separated from MBP via affinity chromatography.