A major challenge in bisulfite sequencing is the degradation of DNA that takes place concurrently with the conversion. The conditions necessary for complete conversion, such as long incubation times, elevated temperature, and high bisulfite concentration, can lead to the degradation of about 90% of the incubated DNA.
In bisulfite sequencing, a significant challenge is the simultaneous degradation of DNA during the conversion process. The conditions needed for complete conversion, including extended incubation times, elevated temperatures, and high concentrations of bisulfite, can result in about 90% degradation of the incubated DNA.