In his will, Infante Francisco de Paula tried to protect his youngest son's inheritance and placed him under the custody of Queen Isabella II. Less than a year after his second widowhood, Infante Francisco de Paula died of colon cancer on 13 August 1865. He had wished to be buried at the church of San Francisco, but he was buried at the Escorial as was due a person of his rank.
In his will, Infante Francisco de Paula attempted to safeguard his youngest son's inheritance and entrusted him to Queen Isabella II. Less than a year after his second widowhood, Infante Francisco de Paula passed away from colon cancer on 13 August 1865. He had intended to be buried at the church of San Francisco but was instead interred at the Escorial as befitting someone of his status.