It has also been argued that the removal of the varnish has left the painting tonally out of balance, since Titian is likely to have added some subtle glazes to the paint surface in order to tone down some of the more jarring colors.
The removal of varnish is argued to have negatively impacted the painting's color balance, as it likely involved the use of glazing techniques by Titian to soften some of the more jarring hues.