Ahead of the 2007–08 season, Tlustý attended the Maple Leafs' prospect camp tournament, where he played in all four tournament games, scoring three goals and adding one assist for four points. After the prospects' camp, Tlustý then attended the Maple Leafs' training camp and participated in the pre-season action. He played five games in the pre-season, recording one assist and eventually making the final cuts before ultimately being assigned to the AHL again.
Prior to the start of the 2007–08 season, Tlustý participated in various events with the Toronto Maple Leafs organization: a prospect camp tournament, training camp, and pre-season games. He ultimately returned to the American Hockey League after being cut from the final roster.