In 1645, learning that La Tour had taken a journey to Quebec, d'Aulnay again laid siege to the fort; but Madame La Tour directed from the bastions the cannonade on the enemy's ships, and compelled d'Aulnay to retire. By the aid of a treacherous sentry, he was enabled, on his third attack, to enter the fort, but the resistance led by Madame La Tour was so fierce that he proposed terms of capitulation, pledging life and liberty to all in the garrison.
In 1645, after learning that La Tour had traveled to Quebec, d'Aulnay renewed his siege on the fort; however, Madame La Tour directed from the bastions a cannonade against the enemy ships and forced d'Aulnay to withdraw. With the aid of a treacherous sentry, he managed to enter the fort for the third time, but the fierce resistance led by Madame La Tour compelled him to offer terms of surrender, promising life and liberty to all in the garrison.