It is produced by glutamate formimidoyltransferase and then converted into 5,10-methenyltetrahydrofolate by formiminotransferase cyclodeaminase.
This compound is synthesized through the action of glutamate formimidoyltransferase and then undergoes a conversion to 5,10-methenyltetrahydrofolate via the activity of formiminotransferase cyclodeaminase.