Thus, at high concentrations, ATP outcompetes the drug. One limitation of traditional coumarins is gyrB ability to confer antibiotic resistance due to mutations and as a result decrease the inhibitor's ability to bind and induce cell death.
High concentrations of ATP can overcome the effects of drugs. A common issue with traditional coumarin compounds is that they can lead to antibiotic resistance, which occurs through mutations, making it harder for the drug to bind and kill cells.