The orderly innervation by auditory nerve fibers gives the AVCN a tonotopic organization along the dorsoventral axis. Fibers that carry information from the apex of the cochlea that are tuned to low frequencies contact neurons in the ventral part of the AVCN; those that carry information from the base of the cochlea that are tuned to high frequencies contact neurons in the dorsal part of the AVCN.
The AVCN's neural connections, facilitated by auditory nerve fibers, exhibit a specific spatial arrangement along its dorsoventral axis. This results in distinct groups of neurons being activated based on their frequency specificity, with those responding to low frequencies located in the ventral portion and those responding to high frequencies situated in the dorsal portion.