It is the EVH1 domains of VASP/EVL proteins that directly contact the FPPPP motif. The precise architecture of TES:MENA binding was revealed by X-ray crystallography, and showed that the 3rd LIM domain of TES covered up the FPPPP binding site within Menas EVH1 domain. Isothermal titration calorimetry showed that TES has a greater affinity for Mena than its canonical FPPPP ligand, as presented in the focal adhesion protein zyxin.
It is the EVH1 domains of VASP/EVL proteins that physically connect to the FPPPP motif. Crystallographic analysis revealed that the 3rd LIM domain of TES obstructs the FPPPP binding site within Menas' EVH1 domain. Thermodynamic studies indicated that TES has a higher affinity for Mena than its standard FPPPP ligand, as seen in zyxin.