By 1672, Barthélemy had joined François de Salignac de la Mothe-Fénelon and Claude Trouvé in a mission to the Iroquois on the north shore of Lake Ontario.
By 1672, Barthélemy had collaborated with François de Salignac de la Mothe-Fénelon and Claude Trouvé in a venture to the Iroquois people situated near Lake Ontario.