Some Gram-positive bacteria also encode a soluble diacylglycerol kinase capable of reintroducing DAG into the phospholipid biosynthesis pathway. DAG accumulates in Gram-positive bacteria as a result of the transfer of glycerol-1-phosphate moieties from phosphatidylglycerol to lipotechoic acid.
In Gram-positive bacteria, some species have a mechanism that reintroduces diacylglycerol into their phospholipid synthesis process. This leads to an accumulation of DAG in these bacteria due to the transfer of glycerol-1-phosphate groups from phosphatidylglycerol to lipotechoic acid.