This step is similar to other small self-cleaving ribozymes such as the Hammerhead ribozyme and HDV ribozyme. Both of those ribozymes generate a product, which contain a 2’, 3’ -cyclic phosphate.
This step is comparable to those employed by other compact self-cleaving ribonucleases, such as the Hammerhead ribozyme and HDV ribozyme. These enzymes produce a product featuring a 2’, 3’ -cyclic phosphodiester bond.