It is an effective buffer between the pH 5.8 and 7.2. Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd.
This buffer has a wide range of effectiveness between pH 5.8 and 7.2. Bis-tris methane forms strong bonds with Cu and Pb ions, as well as weaker connections to Mg, Ca, Mn, Co, Ni, Zn, and Cd.