The DNA-binding domains of TetR recognize a 15 base pair palindromic sequence  of the TetA operator. These domains mainly consist of a helix-turn-helix (HTH) motif that is common in TetR protein family members (see below). However, the N-terminal residues preceding this motif have also been shown to be important for DNA binding.
The TetR protein's DNA-binding capabilities are centered on recognizing a 15 base pair palindromic sequence within the TetA operator. The primary structure of these domains consists mainly of an HTH motif, which is characteristic of TetR family members (see below). Additionally, the N-terminal residues preceding this motif have been found to play a significant role in DNA binding.