The regiment was originally formed as a part of the 135th Aviation Regiment with Regimental Headquarters located in Frankfort, Kentucky, 1st Battalion in Missouri and 2nd Battalion as a part of the Colorado Army National Guard as 2nd Battalion 135th Aviation, under the United States Army Regimental System.
The regiment started as a component of the 135th Aviation Regiment, with its headquarters in Frankfort, Kentucky. The 1st Battalion was situated in Missouri and the 2nd Battalion was part of the Colorado Army National Guard under the United States Army Regimental System as the 2nd Battalion 135th Aviation.