The FIE3 lies within a 201-nucleotide sequence just upstream of the polyadenylation signal and can act autonomously to destabilize a heterologous mRNA. Further deletion constructs identified an essential 68-nucleotide element within the FIE3.
The FIE3 is situated within a 201-nucleotide sequence immediately upstream of the polyadenylation signal and can operate independently to destabilize an extraneous mRNA. Additional deletion constructs revealed an essential 68-nucleotide component within the FIE3.