Each subunit has an active site where the ligands are held. The crystallized structure of ACS was determined with CoA and Adenosine- 5′-propylphosphate bound to the enzyme.
The specific components of ACS have a functional site where substrates are held in place. The solid-state structure of ACS was investigated by incorporating CoA and Adenosine-5′-propylphosphate into the enzyme, providing insight into its function.