Born in Maubeuge in 1601, Desubleo probably learned his trade in Flanders, although there is no proof he was trained in the workshop of Abraham Janssens together with his stepbrother Nicolas Régnier. With the latter he moved to Rome, where he is recorded in 1624 and 1625. By the beginning of the 1630s, he was working in Bologna, in the busy workshop of Guido Reni, who was a crucial influence on him and on other artists of his age or slightly younger, including Simone Cantarini and Jean Boulanger.
Desubleo was born in Maubeuge in 1601 and likely learned his craft in Flanders. There is no concrete evidence that he trained with Abraham Janssens alongside his stepbrother Nicolas Régnier. The two relocated to Rome, where Desubleo's work is documented in 1624 and 1625. By the early 1630s, he had joined Guido Reni's workshop in Bologna, which had a profound impact on him and other artists of similar age or slightly younger.