The overall success of OE-PCR based DNA asseblies relies on several factors, being the most relevant ones the instrinsic features of the DNA sequence to assemble, the sequence and length of the overlapping overhangs, the design of outer primers for the final amplification and the conditions of the PCR reaction.
The overall effectiveness of OE-PCR based DNA assemblies is contingent upon several critical factors, including the inherent characteristics of the DNA sequence being assembled, the length and sequence of the complementary overhangs, the design of the outer primers for the final amplification stage, and the conditions under which the PCR reaction is performed.