When attempting to identify biological molecules that would disrupt the formation of the F complex, researchers developed a high-throughput fluorescence polarization (FP)-binding assay. In this assay, a small peptide of a known sequence was synthesized and tagged with a fluorescent molecule.
To discover biological molecules that would hinder the formation of the F complex, scientists developed a rapid and sensitive assay using fluorescence polarization. A small peptide with a predetermined sequence was synthesized and attached to a fluorescent tag for analysis.