The first half of the 16th century spelled problems in terms of religious service, and it is despite the Medininkai-Bystritsa Augustians receiving new endowments and donations. The discipline within the monastery deteriorated; in 1523 the Canons left Bystritsa, and in 1528 they were removed from Medininkai for "non-compliance with rules of the order"; it was manifested e.g. by drunken episodes and dereliction of duty.
The first half of the 16th century proved difficult for religious service at the Medininkai-Bystritsa Augustians monastery, despite receiving new endowments and donations. Their discipline deteriorated, leading to their expulsion from Bystritsa in 1523 and ultimately removal from Medininkai in 1528 due to "non-compliance with the order's rules," which manifested as instances of excessive drinking and neglect of duties.