In all, 15 thousand signatures were added against the project. After the approval of the bill, the League Against Mandatory Vaccine was founded on 5 November, in a meeting at the Centro das Classes Operárias presided over by Lauro Sodré and Vicente de Souza and with the presence of two thousand people. The League was formed by a coalition of radical republican politicians, ideological factions within the Brazilian Army, and journalists.
A total of 15,000 signatures opposed the project after its approval.  The League Against Mandatory Vaccination was founded on November 5th at a meeting in Centro das Classes Operárias, led by Lauro Sodré and Vicente de Souza with two thousand attendees. The League included radical republican politicians, factions within the Brazilian Army, and journalists.