The pKa declines approximately 0.03 units per degree Celsius rise in temperature. This can lead to relatively dramatic pH shifts when there are shifts in solution temperature. Sodium chloride concentration may vary from 100 to 200 mM, tris concentration from 5 to 100 mM and pH from 7.2 to 8.0.
The pKa decreases by approximately 0.03 units for each degree Celsius increase in temperature. This can result in substantial changes in pH when solution temperatures vary. Sodium chloride concentrations may range from 100 to 200 mM, tris concentrations may be between 5 and 100 mM, and pH levels are usually within the range of 7.2 to 8.0.