Instead, the active site is pre-formed and glmS ribozyme activity is triggered by the introduction of a functional group on GlcN6P that is necessary for catalysis. As mentioned above, the amine group on GlcN6P is thought to be the catalytically involved functional group, which is supported by studies that evaluated glmS ribozyme activity using different ligands, under physiologic conditions.
In contrast, the active site is pre-formed and glmS ribozyme activity is initiated by the introduction of a functional group on GlcN6P that is required for catalysis. As previously stated, the amine group on GlcN6P is believed to be the catalytically involved functional group, which is corroborated by studies evaluating glmS ribozyme activity using various ligands under physiological conditions.