The NBD peptide consists of the region from T735 to E745 of the IKK-β subunit fused with a sequence derived from the Antennapedia homeodomain that mediates membrane translocation. Furthermore, wild type NBD peptide has been shown to dose-dependently inhibit interaction of IKKB with NEMO compared to mutant controls. Additionally, NF-κB activation was suppressed in HeLa cells after incubation with NBD wild type peptides.
The NBD peptide includes a segment from T735 to E745 of the IKK-β subunit combined with a sequence from the Antennapedia homeodomain, which aids in membrane translocation. Wild-type NBD peptides have been shown to dose-dependently inhibit interactions between IKKB and NEMO compared to mutant controls. NF-κB activation has also been suppressed in HeLa cells after incubation with wild-type NBD peptides.