By expressing a recombinant form of the N-terminal domain I of the protein and demonstrating that digestion of the peptide with either heparanase or chondroitinase did not lead to complete loss of the peptide's activity, it was shown that chondroitin sulfate chains can be added to human perlecan.
Expressing a recombinant form of the N-terminal domain I of the protein, demonstrating that digestion with heparanase or chondroitinase did not result in complete loss of peptide activity, it was shown that chondroitin sulfate chains can be attached to human perlecan.