It is the EVH1 domains of VASP/EVL proteins that directly contact the FPPPP motif. The precise architecture of TES:MENA binding was revealed by X-ray crystallography, and showed that the 3rd LIM domain of TES covered up the FPPPP binding site within Menas EVH1 domain. Isothermal titration calorimetry showed that TES has a greater affinity for Mena than its canonical FPPPP ligand, as presented in the focal adhesion protein zyxin.
It is the EVH1 domains of VASP/EVL proteins that directly make contact with the FPPPP motif. The precise structure of TES:MENA binding was clarified by X-ray crystallography, which demonstrated that the 3rd LIM domain of TES covered up the FPPPP binding site within Menas' EVH1 domain. Isothermal titration calorimetry revealed that TES has a greater affinity for Mena than its conventional FPPPP ligand, as seen in the focal adhesion protein zyxin.