Heteroduplex analysis (HDA) is a method in biochemistry used to detect point mutations in DNA (Deoxyribonucleic acid) since 1992. Heteroduplexes are dsDNA molecules that have one or more mismatched pairs, on the other hand homoduplexes are dsDNA which are perfectly paired. This method of analysis depend up on the fact that heteroduplexes shows reduced mobility relative to the homoduplex DNA.
Heteroduplex analysis (HDA) is a biochemical method used to detect point mutations in DNA (Deoxyribonucleic acid) since 1992. Heteroduplex molecules are formed by dsDNA with mismatched base pairs, whereas homoduplexes have perfectly paired dsDNA. This technique relies on the difference in mobility between heteroduplex and homoduplex DNA.