Surprisingly, they isolated a very large multi-sub-unit supra-molecular complex, instead of a small protein. A combination of biochemical, biophysical, immun-ochemical and ultra-structural techniques, followed by molecular biological verification, led to the definition and proof of the cellulosome concept. The birth of the discrete, multi-enzyme cellulosome complex was thus documented.
Remarkably, they discovered a very large multi-sub-unit supra-molecular complex, rather than a small protein. A combination of biochemical, biophysical, immunochemical, and ultra-structural techniques, followed by molecular biological verification, led to the definition and proof of the cellulosome concept. The emergence of the discrete, multi-enzyme cellulosome complex was thus documented.