Rama reported back to his office on February 8, 2016, as the 60-day suspension imposed on him by the Office of the President of the Philippines had ended. Rama ran for a third and final consecutive term in 2016, but was defeated by Osmeña by a margin of 33,894 votes.
Following his 60-day suspension from February 8, 2016, Rama resumed work at his office and then proceeded to run for re-election in 2016. However, he ultimately lost the election to Osmeña with a substantial difference of 33,894 votes.