Additionally, RNA polymerase contains a core Mg+ ion that assists the enzyme with its catalytic properties. RNA polymerase works by catalyzing the nucleophilic attack of 3’ OH of RNA to the alpha phosphate of a complementary NTP molecule to create a growing strand of RNA from the template strand of DNA. Furthermore, RNA polymerase also displays exonuclease activities, meaning that if improper base pairing is detected, it can cut out the incorrect bases and replace them with the proper, correct one.
Additionally, RNA polymerase includes a central Mg+ ion that aids in its catalytic functions. RNA polymerase operates by facilitating the nucleophilic attack of 3’ OH on an RNA molecule to the alpha phosphate of a complementary NTP to generate a growing RNA strand from the template DNA strand. Furthermore, RNA polymerase exhibits exonuclease activities, indicating it can remove incorrect bases and replace them with correct ones if there is improper base pairing.