Chromosome combing is also used to study DNA replication, a highly regulated process that is reliant on a specific program of temporal and spatial distribution of activation of origins of replication. Each origin occupies a distinct genetic locus and must fire only once per cell cycle.
Chromosome combing is utilized in addition to studying the process of DNA replication, which involves a highly orchestrated sequence of events involving the activation of replication origins at specific times and locations within the cell. Each origin site is distinct and can only be activated once during each cell division.