(2011) developed a much more efficient method for the production of RNAi vectors in which expression of the RNAi can be knocked-in using Cre-recombinase and knocked-out using Flp-recombinase. The novel GRIM Expression System allows for the much faster generation of expression vectors containing artificial RNAi constructs. The authors went on to show that their expression system works quite effectively in human embryonic kidneys (HEK) cells, a common human immortalized cell line in molecular research.
(2011) developed a more efficient method for producing RNAi vectors, enabling expression of RNAi to be turned on using Cre-recombinase and off using Flp-recombinase. The novel GRIM Expression System allows for the rapid creation of expression vectors containing artificial RNAi constructs. The authors demonstrated that their system functions effectively in human embryonic kidney (HEK) cells, a widely used immortalized cell line in molecular research.