Additionally, the formation of riboflavin from 6,7-dimethyl-8-ribityllumazine can occur in boiling aqueous solution in the absence riboflavin synthase.
Furthermore, under certain conditions, riboflavin can be synthesized from 6,7-dimethyl-8-ribityllumazine through a chemical reaction that takes place in boiling water, even in the absence of the enzyme riboflavin synthase.