Spontaneous isopeptide bond formation has been exploited in the development a peptide tag called SpyTag. SpyTag can spontaneously and irreversibly react with its binding partner (a protein termed SpyCatcher) through a covalent isopeptide bond.
Spontaneous isopeptide bond formation has been utilized in the development of a peptide tag called SpyTag. SpyCatcher, a protein that binds to SpyTag through a covalent isopeptide bond, is used to create a stable and irreversible connection.