Cuffe proceeded B.A. 13 June 1580, and was elected fellow of his college 30 May 1583, but a severe remark about the practical jokes which the founder of Trinity, Sir Thomas Pope, was fond of playing on his friends, led to his expulsion from the college. In 1586, Sir Henry Savile offered him a tutorship at Merton, and there Cuffe pursued his Greek studies with conspicuous success.
Cuffe was admitted to his college on June 13, 1580, and became a fellow of the college on May 30, 1583. However, a harsh remark about Sir Thomas Pope's pranks led to his expulsion from the college. In 1586, Sir Henry Savile offered Cuffe a position as tutor at Merton, where he excelled in Greek studies.