Generally, the steps for applying modeling software towards MFA include metabolic reconstruction to compile all desired enzymatic reactions and metabolites, provide experimental information such as the labeling pattern of the substrate, define constraints such as growth equations, and minimizing the error between the experimental and simulated results to obtain final fluxes.
Typically, the steps for utilizing modeling software in MFA involve metabolic reconstruction to compile all necessary enzymatic reactions and metabolites, incorporate experimental data such as substrate labeling patterns, establish limitations like growth equations, and minimizing the discrepancy between experimental and simulated outcomes to derive final fluxes.