In CF, genomic DNA is heat-denatured and allowed to renature to a Cot value (Cot = DNA concentration x time x a factor based on the cation concentration of the buffer) at which the majority of repetitive elements have reassociated but single and low-copy elements remain single stranded.
In CF, genomic DNA is denatured by heat and allowed to reanneal at a Cot value (Cot = DNA concentration x time x a factor based on the cation concentration of the buffer) where most repetitive elements have rejoined but single and low-copy elements remain single stranded.