2 September 1642 was the day that every theatre company was outlawed from performing. There is not much else that is mentioned about Beeston's Boys after this time, but it is suspected that the company continued acting in the 1650s since there is a reference of William Beeston training another group of boys to take the stage.
On September 2, 1642, all theatre companies were banned from performing. After this date, information about Beeston's Boys is scarce, but it is believed they continued acting in the 1650s as William Beeston trained another group of boys to perform on stage.