In the United States military, the phrase is the motto of the US Army's oldest infantry regiment, the 3rd U.S. Infantry Regiment (The Old Guard), located at Fort Myer, Virginia. "Don't tread on me" is also used in the First Navy Jack of United States Navy.
"Don't tread on me" is a phrase that signifies resistance to oppression or interference. It has been adopted by both the United States Army and the United States Navy.