By 1672, Barthélemy had joined François de Salignac de la Mothe-Fénelon and Claude Trouvé in a mission to the Iroquois on the north shore of Lake Ontario.
In 1672, Barthélemy teamed up with François de Salignac de la Mothe-Fénelon and Claude Trouvé on an expedition to the Iroquois tribe along the northern shore of Lake Ontario.