CHO Umurongo Utugari Utanga Serivisi yihariye
HEK293T (HEK293 yahinduwe) umurongo w'ingirabuzimafatizo ni umurongo w'impyiko w'umuntu ukomoka ku isoro ry'umuntu mu myaka ya za 70.Ikoreshwa muburyo butandukanye bwubushakashatsi kandi nigikoresho cyingenzi mukwiga imvugo ya gene, imiterere ya protein n'imikorere, kwanduza ibimenyetso, no kuvumbura ibiyobyabwenge.Ingirabuzimafatizo ziroroshye kwandura kandi zisanzwe zikoreshwa mukwiga ingaruka ziterwa na genoside zitandukanye, nko gukabya gukabije cyangwa gukomeretsa ingirabuzimafatizo zitandukanye, kuri fenotipi y'utugari.Utugingo ngengabuzima twakoreshejwe kandi mu bushakashatsi bw'ibinyabuzima bikomoka ku ngirabuzimafatizo, ibinyabuzima bya kanseri, na immunologiya.
Ibicuruzwa birambuye
Ibicuruzwa
Umuco Wibanze
Umuco wibanze wimikorere ninzira ikoreshwa mugukura no kubungabunga selile muri vitro kuva selile imwe cyangwa cluster ya selile.Ubu buryo bukoreshwa mukwiga imyitwarire nimiterere ya selile, kandi birashobora gukoreshwa muburyo butandukanye, harimo gupima ibiyobyabwenge, ubushakashatsi mubuvuzi, hamwe nubuvuzi bushingiye ku ngirabuzimafatizo.Umuco wibanze wibanze ukorerwa mubidukikije bigenzurwa, mubisanzwe ku ntebe ya laboratoire, kandi bigashyigikirwa nibikoresho byihariye na reagent.Ingirabuzimafatizo zigumana ubuzima zitanga intungamubiri zikenewe kandi zigakomeza ubushyuhe bukwiye, pH, na ogisijeni.Ingirabuzimafatizo nazo zikurikiranwa ku bimenyetso byose byerekana imihangayiko cyangwa umwanda, kandi umuco ugenzurwa buri gihe niba hari impinduka zikura cyangwa imitekerereze.
Akagari ka muntu
Ingirabuzimafatizo yumuntu nigice cyibanze cyubuzima.Umubiri wumuntu ugizwe na trillioni yingirabuzimafatizo buriwese ufite imiterere numurimo wihariye.Ingirabuzimafatizo zubaka ibinyabuzima byose kandi zifite inshingano zo gukura, metabolism, nibindi bikorwa byingenzi.Ingirabuzimafatizo zigizwe nibice bitandukanye, birimo proteyine, ADN, karubone, lipide, na organelles.
Umuco w'Akagari Yisumbuye
Umuco w'akagari ka kabiri ni inzira yo guhuza ingirabuzimafatizo zitaruye kandi zikurira muri laboratoire mbere.Ingirabuzimafatizo zirashobora gukura zivuye mubisobanuro byama tissue, bitandukanijwe na enzymes, cyangwa bigakoronizwa kuva selile imwe.Umuco w'akagari ka kabiri ukoreshwa mu kwagura imirongo y'utugari, kwiga imyitwarire y'utugari, no guteza imbere ingirabuzimafatizo.Ubwoko bwimikorere isanzwe ikoreshwa mumico ya kabiri ya selile harimo fibroblast, selile endothelia, na selile selile.